公开(公告)号：US20200003743A1

公开(公告)日：2020-01-02

申请号：US16476906

申请日：2018-01-11

Applicant: Keygene N.V.

Inventor： Marco Gerardus Maria van Schriek ,  Rudolf Laurentius van Bavel

IPC: G01N33/00 ,  G06T19/00

Abstract: A system for monitoring plants comprises an input unit, an output unit, and a processor that provides (201) image data (107) for a plurality of plants, each image data being associated with an individual plant. It associates (204) the image data (107) of each plant with corresponding plant characteristic data (109). It selects (206) a subset of the plants based on the plant characteristic data (109). It generates (207) a plurality of computer graphics objects corresponding to the selected plants. It applies (208) the image data (107) of each selected plant in form of a computer graphics texture to the corresponding computer graphics object. It determines (209) a position of each computer graphics object in a three-dimensional space of a computer graphics scene. It creates (210) a computer graphics rendering of the scene. It displays (211) the computer graphics rendering.

公开(公告)号：US20190153534A1

公开(公告)日：2019-05-23

申请号：US16267123

申请日：2019-02-04

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Adrianus Johannes Van Tunen

IPC: C12Q1/6874 ,  G16B30/00 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6858 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6844 ,  C12Q1/6827

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

公开(公告)号：US20190024107A1

公开(公告)日：2019-01-24

申请号：US16069970

申请日：2017-01-12

Applicant: KEYGENE N.V.

Inventor： Jesse David Munkvold ,  Anne Deslattes Mays

IPC: C12N15/82

Abstract: The present invention relates to a method for modifying lateral budding in a plant comprising modifying the expression or function of a protein comprising the sequence shown as SEQ ID NO: 3, 4 or 5 or a sequence which has at least 70% sequence identity thereto. The invention further relates to plants, plant propagation material, harvested leaf and processed leaf obtainable by such methods.

公开(公告)号：US10095832B2

公开(公告)日：2018-10-09

申请号：US15862956

申请日：2018-01-05

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Henricus Johannes Adam Van Der Poel

IPC: C12P19/34 ,  G06F19/22 ,  C40B30/04 ,  C12Q1/6827 ,  C12N15/10 ,  C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6883 ,  C12Q1/6858 ,  C40B30/10

Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.

公开(公告)号：US20180025111A1

公开(公告)日：2018-01-25

申请号：US15729328

申请日：2017-10-10

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Henricus Johannes Adam VAN DER POEL

IPC: G06F19/22 ,  C12Q1/68

CPC classification number: G06F19/22 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156 ,  C40B30/04 ,  C40B30/10 ,  C12Q2539/107 ,  C12Q2531/113 ,  C12Q2525/191

Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.

公开(公告)号：US09777324B2

公开(公告)日：2017-10-03

申请号：US15136224

申请日：2016-04-22

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Anker Preben Sørensen ,  Marco Gerardus Maria Van Schriek

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2565/301 ,  C12Q2535/138 ,  C12Q2535/122 ,  C12Q2527/107

Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.

公开(公告)号：US09745627B2

公开(公告)日：2017-08-29

申请号：US15434801

申请日：2017-02-16

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia van Eijk ,  Adrianus Johannes van Tunen

IPC: C12P19/34 ,  C12Q1/68 ,  G06F19/22

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2600/13 ,  G06F19/22 ,  C12Q2525/155 ,  C12Q2563/179 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

公开(公告)号：US20170240916A1

公开(公告)日：2017-08-24

申请号：US15448979

申请日：2017-03-03

Applicant: KEYGENE N.V.

Inventor： Paul BUNDOCK

IPC: C12N15/82 ,  C12N9/10

CPC classification number: C12N15/8275 ,  C12N9/1092 ,  C12Y205/01019

Abstract: The present invention relates to a new method for improving glyphosate resistance of a plant. The method encompasses providing one or more specific mutations in a specific nucleotide sequence, in a said plant. In comparison to a plant not manipulated according to the method, the plant obtained by the method displays (improved) glyphosate resistance. Also provided are a (transgenic) plant including a seed thereof and a plant product that can be obtained by the method according to the invention.

公开(公告)号：US20170166962A1

公开(公告)日：2017-06-15

申请号：US15434788

申请日：2017-02-16

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia van EIJK ,  Adrianus Johannes van TUNEN

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2600/13 ,  G16B30/00 ,  C12Q2525/155 ,  C12Q2563/179 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

公开(公告)号：US09657335B2

公开(公告)日：2017-05-23

申请号：US15288253

申请日：2016-10-07

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Adrianus Johannes Van Tunen

IPC: C12Q1/68 ,  G06F19/22

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2600/13 ,  G06F19/22 ,  C12Q2525/155 ,  C12Q2563/179 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.