公开(公告)号：US07943314B2

公开(公告)日：2011-05-17

申请号：US11993986

申请日：2006-07-03

Applicant: Stuart Wilson ,  Sharon Banin

Inventor： Stuart Wilson ,  Sharon Banin

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/25 ,  C12Q1/68 ,  C12Q1/6844 ,  Y02A50/52 ,  C12Q2563/143 ,  C12Q2561/125 ,  C12Q2521/543

Abstract: A method of detecting a molecule associated with viability of one or more cells or organisms in a sample comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase. The method has numerous applications, in particular in monitoring viability of cells, toxicology testing and determining whether there is contamination in a sample or on a surface. Kits are also provided for carrying out the methods.

Abstract translation: 检测与样品中一种或多种细胞或生物体的活力相关的分子的方法包括使样品与酶接触的初始步骤，该酶能够在或从其中的核酸分子中加入或除去化学部分 与一种或多种细胞或生物的活力相关的分子的存在。 由此产生新的可检测的核酸分子。 下一步涉及通过检测仅在与一种或多种细胞或生物体的活力相关的分子的存在下产生的新的核酸分子来检测与一种或多种细胞或生物体的存活力相关的分子的存在。 与活力相关的最优选的分子是ATP，尽管也可以检测到NAD。 用于该方法的优选酶是连接酶。 该方法具有许多应用，特别是在监测细胞的活力，毒理学测试和确定样品或表面是否存在污染。 还提供工具包来执行这些方法。

公开(公告)号：US20090098539A1

公开(公告)日：2009-04-16

申请号：US11914697

申请日：2006-05-18

Applicant: Stuart Wilson ,  John Christopher Stanley ,  Sharon Banin

Inventor： Stuart Wilson ,  John Christopher Stanley ,  Sharon Banin

IPC: C12Q1/68

CPC classification number: G01N33/57434 ,  C12Q1/42 ,  C12Q1/682 ,  G01N33/573 ,  C12Q2563/179 ,  C12Q2521/525

Abstract: An improved method of detecting an enzyme in a sample, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, thereby conferring the nucleic acid molecule with the ability to be extended to generate a novel detectable nucleic acid molecule, comprises the steps of allowing the sample to be tested for the presence of the enzyme to interact with the nucleic acid molecule; and testing for interaction of the enzyme with the nucleic acid molecule by detecting the novel nucleic acid molecule generated only in the presence of the enzyme. The preferred enzyme is a phosphatase. The methods have a number of applications, for example in enhancing the sensitivity of immunoassays, for detecting pathogen associated phosphatase, for diagnosing certain conditions and for detecting specific contaminants in a sample.

Abstract translation: 一种检测样品中酶的改进方法，该酶能够向或从核酸分子添加或除去化学部分，从而赋予具有延伸以产生新的可检测核酸分子的能力的核酸分子 包括以下步骤：使样品测试酶的存在与核酸分子相互作用; 并通过检测仅在酶存在下产生的新的核酸分子来测试酶与核酸分子的相互作用。 优选的酶是磷酸酶。 该方法具有许多应用，例如增强免疫测定的灵敏度，用于检测病原体相关磷酸酶，用于诊断某些条件并检测样品中的特定污染物。

公开(公告)号：US20090047132A1

公开(公告)日：2009-02-19

申请号：US11839629

申请日：2007-08-16

Applicant: Shawn P. Riley ,  Kazim Ozbaysal ,  Paul Stuart Wilson ,  David Edwin Budiner

Inventor： Shawn P. Riley ,  Kazim Ozbaysal ,  Paul Stuart Wilson ,  David Edwin Budiner

IPC: B64C11/20 ,  B23P6/00

CPC classification number: F01D5/225 ,  B23P6/005 ,  F01D5/005 ,  F01D5/16 ,  F05D2230/80 ,  Y10T29/49318 ,  Y10T29/49336

Abstract: A durable blade, a method of manufacturing it and a method of repairing blades are described and claimed. Durable blade includes an airfoil having a pressure side and a suction side, a midspan shroud located on the airfoil, at least one recess on the midspan shroud and a wear pad attached to the recess of the midspan shroud.

Abstract translation: 描述和要求保护耐用的刀片，其制造方法和修理刀片的方法。 耐用刀片包括具有压力侧和吸力侧的翼型件，位于翼型上的中跨护罩，中跨护罩上的至少一个凹部和附接到中跨护罩的凹部的耐磨垫。

公开(公告)号：US20050221349A1

公开(公告)日：2005-10-06

申请号：US10998503

申请日：2004-11-29

Applicant: Stuart Wilson ,  Christopher Stanley

Inventor： Stuart Wilson ,  Christopher Stanley

IPC: C12Q1/68 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543

CPC classification number: C12Q1/6804 ,  G01N33/54306

Abstract: Methods and kits for detecting target molecules or for monitoring interactions between target molecules. In particular, the invention relates to methods based on the use of “two-component conjugate” systems comprising a catalytic label and a substrate label.

Abstract translation: 用于检测靶分子或用于监测靶分子之间的相互作用的方法和试剂盒。 特别地，本发明涉及基于使用包含催化标记和底物标记的“双组分缀合物”系统的方法。

公开(公告)号：US20050181544A1

公开(公告)日：2005-08-18

申请号：US11014439

申请日：2004-12-16

Applicant: Belgacem Haba ,  Masud Beroz ,  Ronald Green ,  Ilyas Mohammed ,  Stuart Wilson ,  Wael Zohni ,  Yoichi Kubota ,  Jesse Thompson

Inventor： Belgacem Haba ,  Masud Beroz ,  Ronald Green ,  Ilyas Mohammed ,  Stuart Wilson ,  Wael Zohni ,  Yoichi Kubota ,  Jesse Thompson

IPC: H01L21/44

CPC classification number: H01L23/49811 ,  G01R1/06744 ,  G01R1/0735 ,  H01L21/563 ,  H01L23/4985 ,  H01L24/12 ,  H01L24/16 ,  H01L24/48 ,  H01L24/73 ,  H01L2224/0401 ,  H01L2224/05599 ,  H01L2224/0603 ,  H01L2224/131 ,  H01L2224/13147 ,  H01L2224/13187 ,  H01L2224/13188 ,  H01L2224/1319 ,  H01L2224/1329 ,  H01L2224/133 ,  H01L2224/1357 ,  H01L2224/136 ,  H01L2224/1403 ,  H01L2224/16 ,  H01L2224/17 ,  H01L2224/1703 ,  H01L2224/48091 ,  H01L2224/73203 ,  H01L2224/73204 ,  H01L2224/73257 ,  H01L2224/73265 ,  H01L2224/83194 ,  H01L2224/85399 ,  H01L2924/00013 ,  H01L2924/00014 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01027 ,  H01L2924/01028 ,  H01L2924/01029 ,  H01L2924/01033 ,  H01L2924/01074 ,  H01L2924/01078 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/01084 ,  H01L2924/01322 ,  H01L2924/014 ,  H01L2924/09701 ,  H01L2924/12042 ,  H01L2924/14 ,  H01L2924/181 ,  H01L2924/19041 ,  H01L2924/3011 ,  H01L2224/13099 ,  H01L2224/45099 ,  H01L2924/00 ,  H01L2924/00012

Abstract: A microelectronic package includes a microelectronic element having faces and contacts and a flexible substrate spaced from and overlying a first face of the microelectronic element. The package also includes a plurality of conductive posts extending from the flexible substrate and projecting away from the first face of the microelectronic element, wherein at least some of the conductive posts are electrically interconnected with the microelectronic element, and a plurality of support elements supporting the flexible substrate over the microelectronic element. The conductive posts are offset from the support elements to facilitate flexure of the substrate and movement of the posts relative to the microelectronic element.

Abstract translation: 微电子封装包括具有面和触点的微电子元件和与微电子元件的第一面间隔开并且覆盖其上的柔性基板。 该封装还包括从柔性基板延伸并从远离微电子元件的第一面突出的多个导电柱，其中至少一些导电柱与微电子元件电互连，以及多个支撑元件 微电子元件上的柔性衬底。 导电柱从支撑元件偏移以便于基板的挠曲和柱相对于微电子元件的移动。

公开(公告)号：US20050166530A1

公开(公告)日：2005-08-04

申请号：US10761649

申请日：2004-01-14

Applicant: Stuart Wilson

Inventor： Stuart Wilson

IPC: E04C3/292 ,  E04C3/30

CPC classification number: E04C3/292

Abstract: A structural beam has a thin metal web with a plurality of holes located along its top and bottom edges. One or more rectangular flange pieces are located on each side of the web overlying its top margin and one or more rectangular flange pieces are located on each side of the web overlying its bottom margin. The flange pieces contain holes which are aligned with the holes in the web piece and fasteners extend through the aligned holes to fasten the flange pieces to the web.

Abstract translation: 结构梁具有薄金属腹板，沿着其顶部和底部边缘设有多个孔。 一个或多个矩形凸缘片位于幅材的上边缘的每一侧上，并且一个或多个矩形凸缘片位于幅材的每一侧上，覆盖其底部边缘。 凸缘件包含与网片中的孔对准的孔，并且紧固件延伸穿过对准的孔，以将凸缘件紧固到网。

公开(公告)号：US06913372B2

公开(公告)日：2005-07-05

申请号：US10221939

申请日：2001-03-19

Applicant: Michael Allcock ,  Stuart Wilson

Inventor： Michael Allcock ,  Stuart Wilson

IPC: F21S8/04 ,  F21V5/02 ,  F21V5/00

CPC classification number: F21S8/04 ,  F21V5/02 ,  F21Y2103/00 ,  F21Y2113/00

Abstract: The assembly has two lamps, such as fluorescent tubes. A cover is a co-extrusion having a baffle between the lamps and shroud portions outermost of the cover. The baffle blocks at least some of the light output of the lamps from crossing the region between the lamps. This helps reduce glare, which is further reduced by the configuration of the shrouds.

Abstract translation: 该组件有两个灯，如荧光灯管。 盖是共挤出件，其在灯和护套外壳之间具有挡板。 挡板阻挡灯的至少一些光输出从灯之间的区域穿过。 这有助于减少眩光，这进一步减少了护罩的配置。

公开(公告)号：US06789325B2

公开(公告)日：2004-09-14

申请号：US09954485

申请日：2001-09-17

Applicant: Leslie Peter Stuart Wilson

Inventor： Leslie Peter Stuart Wilson

IPC: B67B748

CPC classification number: B67B7/34

Abstract: A can opener has a first and a second handle member pivotably connected to be moveable between an open disposition and a closed disposition. They can be squeezed together in one hand of the operator to rotate a drive wheel. A cutting wheel is mounted onto the first handle member. A radially facing aperture is formed through a part of said first handle member so that the drive wheel may partly protrude to engage the rim of the can and press it against the cutting wheel. The drive wheel is mounted rotatably onto a rivet which is held slideably in a slot in the main plate of the first handle member to be moveable towards and away from the cutting wheel.

Abstract translation: 开罐器具有第一和第二手柄构件，该第一和第二手柄构件可枢转地连接以在打开配置和封闭配置之间移动。 它们可以在操作者的一只手中挤压在一起以旋转驱动轮。 切割轮安装在第一手柄部件上。 径向面对的孔通过所述第一把手构件的一部分形成，使得驱动轮可以部分地突出以接合罐的边缘并将其压靠在切割轮上。 驱动轮可旋转地安装在铆钉上，该铆钉可滑动地保持在第一手柄构件的主板中的狭槽中，以能够朝向和远离切割轮移动。

公开(公告)号：US5660524A

公开(公告)日：1997-08-26

申请号：US912440

申请日：1992-07-13

Applicant: Ching-Pang Lee ,  Anne Marie Isburgh ,  Donald George La Chapelle ,  Paul Stuart Wilson

Inventor： Ching-Pang Lee ,  Anne Marie Isburgh ,  Donald George La Chapelle ,  Paul Stuart Wilson

IPC: F01D5/18

CPC classification number: F01D5/186 ,  F01D5/182 ,  F01D5/187 ,  F05D2260/201 ,  F05D2260/202 ,  Y02T50/676

Abstract: An airfoil blade, such as a jet engine turbine rotor blade. An internal serpentine coolant circuit has a last downstream passageway bounded by four monolithic inner walls which are monolithic with at least a portion of the outer walls. Two of the inner walls are spaced from the outer walls and contain air impingement orifices creating two impingement chambers. Some coolant in the serpentine circuit exits the airfoil blade through a coolant exit in the blade tip. The remaining coolant in the circuit passes through the impingement orifices and exits the blade through film cooling holes in the outer walls.

Abstract translation: 翼型叶片，例如喷气发动机涡轮转子叶片。 内部蛇形冷却剂回路具有由四个整体内壁限定的最后一个下游通道，其与外壁的至少一部分是整体的。 两个内壁与外壁间隔开并且包含产生两个冲击室的空气冲击孔。 蛇形回路中的一些冷却剂通过叶片尖端中的冷却剂出口离开翼型叶片。 电路中剩余的冷却剂通过冲击孔，并通过外壁上的薄膜冷却孔离开叶片。

公开(公告)号：US5653110A

公开(公告)日：1997-08-05

申请号：US733892

申请日：1991-07-22

Applicant: Ching-Pang Lee ,  Nesim Abuaf ,  Paul Stuart Wilson

Inventor： Ching-Pang Lee ,  Nesim Abuaf ,  Paul Stuart Wilson

IPC: F01D5/18 ,  F02C1/00 ,  B63H1/14

CPC classification number: F01D5/186 ,  F05D2260/202 ,  F05D2260/2212 ,  Y02T50/676

Abstract: A jet engine component, such as an aircraft gas turbine engine rotor blade or a scramjet engine fuel injector. The component has a wall portion including a first surface exposable to a cooler, higher static pressure fluid and a second surface exposable to a hotter, lower static pressure gas flow flowing across the second surface. The component further includes a generally straight film coolant passageway having an inlet on the first surface and an outlet on the second surface. The second surface has a seamless groove which is open substantially entirely along its longitudinal dimension extending from the outlet along the lower static pressure gas flow for improved film cooling of the second surface.

Abstract translation: 喷气发动机部件，例如飞机燃气涡轮发动机转子叶片或冲击式发动机燃料喷射器。 该部件具有壁部分，其包括可暴露于冷却器的第一表面，较高的静压流体和可暴露于流过第二表面的较热的较低静态气流的第二表面。 该部件还包括大致直的薄膜冷却剂通道，其具有在第一表面上的入口和在第二表面上的出口。 第二表面具有无缝槽，其基本上完全沿着其沿着下部静压气流从出口延伸的纵向尺寸敞开，以改善第二表面的膜冷却。