公开(公告)号：US11821035B1

公开(公告)日：2023-11-21

申请号：US17162991

申请日：2021-01-29

Applicant: 10x Genomics, Inc.

Inventor： Zachary Bent ,  Marlon Stoeckius

IPC: C12Q1/6876

CPC classification number: C12Q1/6876

Abstract: Provided herein are methods of detecting target nucleic acids and uses of the same.

公开(公告)号：US20230340576A1

公开(公告)日：2023-10-26

申请号：US18302421

申请日：2023-04-18

Applicant: 10x Genomics, Inc.

Inventor： Layla Katiraee ,  Michael Schnall-Levin ,  Christina Galonska ,  Marlon Stoeckius

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/154

Abstract: Provided herein are methods of identifying a methylation status of an analyte in a biological sample. Also provided herein are methods that combine identifying the methylation status with spatial technology to identify the location of a methylation status in a biological sample.

公开(公告)号：US11702698B2

公开(公告)日：2023-07-18

申请号：US17573126

申请日：2022-01-11

Applicant: 10x Genomics, Inc.

Inventor： Marlon Stoeckius

IPC: C12Q1/6841 ,  C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6876 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12Q2521/327 ,  C12Q2521/501 ,  C12Q2523/109 ,  C12Q2525/161 ,  C12Q2537/163

Abstract: Methods for enhancing specificity of an analyte binding moiety or probe oligonucleotide to an analyte are provided herein. For example, methods provided herein include blocking a capture binding domain, thereby preventing hybridization to the capture domain of the capture probe affixed to a substrate. Further methods include releasing the block from the capture binding domain, thereby allowing the capture binding domain to specifically bind to the capture domain of the capture probe on the substrate.

公开(公告)号：US20230160008A1

公开(公告)日：2023-05-25

申请号：US18100133

申请日：2023-01-23

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

CPC classification number: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20230159995A1

公开(公告)日：2023-05-25

申请号：US17869636

申请日：2022-07-20

Applicant: 10x Genomics, Inc.

Inventor： Eswar Prasad Ramachandran Iyer ,  Tarjei Sigurd Mikkelsen ,  Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Marlon Stoeckius ,  James Michael Chell ,  Cedric Uytingco

IPC: C12Q1/6841 ,  C12N15/10 ,  C12Q1/6804 ,  G01N33/53

CPC classification number: C12Q1/6841 ,  C12N15/1065 ,  C12Q1/6804 ,  G01N33/5308

Abstract: This disclosure relates to methods for spatial profiling of analytes present in a biological sample. Also provided are methods for using spatially barcoded substrates to detect a biological analyte in a cell culture, an organism, and organoid. Also provided are methods for using spatially barcoded substrates to detect the temporal profile of a biological analyte.

公开(公告)号：US20230143569A1

公开(公告)日：2023-05-11

申请号：US17869675

申请日：2022-07-20

Applicant: 10x Genomics, Inc.

Inventor： Eswar Prasad Ramachandran Iyer ,  Tarjei Sigurd Mikkelsen ,  Augusto Manuel Tentori ,  Rajiv Bharadwaj ,  Marlon Stoeckius ,  James Michael Chell ,  Cedric Uytingco

IPC: C12N15/10 ,  C12Q1/6841 ,  G01N33/53 ,  C12Q1/6804

CPC classification number: C12N15/1065 ,  C12Q1/6804 ,  C12Q1/6841 ,  G01N33/5308

Abstract: This disclosure relates to methods for spatial profiling of analytes present in a biological sample. Also provided are methods for using spatially barcoded substrates to detect a biological analyte in a cell culture, an organism, and organoid. Also provided are methods for using spatially barcoded substrates to detect the temporal profile of a biological analyte.

公开(公告)号：US20230033960A1

公开(公告)日：2023-02-02

申请号：US17963749

申请日：2022-10-11

Applicant: 10x Genomics, Inc.

Inventor： Caroline Julie Gallant ,  Marlon Stoeckius ,  Katherine Pfeiffer

IPC: C12N15/10 ,  C12Q1/6837 ,  C12Q1/6874

Abstract: Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

公开(公告)号：US11505828B2

公开(公告)日：2022-11-22

申请号：US17220534

申请日：2021-04-01

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Layla Katiraee

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20220282329A1

公开(公告)日：2022-09-08

申请号：US17707189

申请日：2022-03-29

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20220127672A1

公开(公告)日：2022-04-28

申请号：US17573126

申请日：2022-01-11

Applicant: 10x Genomics, Inc.

Inventor： Marlon Stoeckius

IPC: C12Q1/6876 ,  C12Q1/6874

Abstract: Methods for enhancing specificity of an analyte binding moiety or probe oligonucleotide to an analyte are provided herein. For example, methods provided herein include blocking a capture binding domain, thereby preventing hybridization to the capture domain of the capture probe affixed to a substrate. Further methods include releasing the block from the capture binding domain, thereby allowing the capture binding domain to specifically bind to the capture domain of the capture probe on the substrate.