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41.发明授权Process for efficiently producing transglutaminase through DNA recombination 失效通过DNA重组有效生产转谷氨酰胺酶的方法
公开(公告)号：US5827712A
公开(公告)日：1998-10-27
申请号：US649193
申请日：1996-05-17
CPC分类号： C12N9/1044 , C07K14/245 , C12N15/67 , Y10S530/825
摘要： The present invention relates to a process for producing a transglutaminase, which comprises incubating Escherichia coli expressing genes encoding a heat shock protein (DnaJ) and a transglutaminase. The transglutaminase is produced in large quantities, at low cost and has the appropriate stereostructure to render the transglutaminase biologically active. The transglutaminase so produced is useful in the food industry.
摘要翻译： 本发明涉及一种转谷氨酰胺酶的制备方法，其包括将表达编码热休克蛋白（DnaJ）和转谷氨酰胺酶的基因表达的大肠杆菌。 转谷氨酰胺酶以低成本大量生产并具有合适的立体结构以使转谷氨酰胺酶具有生物活性。 如此生产的转谷氨酰胺酶在食品工业中很有用。
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公开(公告)号：US09062291B2
公开(公告)日：2015-06-23
申请号：US12541220
申请日：2009-08-14
申请人： Keiichi Yokoyama , Mototaka Suzuki , Tatsuki Kashiwagi , Eiichiro Suzuki , Masayo Date , Seiichi Taguchi
发明人： Keiichi Yokoyama , Mototaka Suzuki , Tatsuki Kashiwagi , Eiichiro Suzuki , Masayo Date , Seiichi Taguchi
IPC分类号： C12N9/10
CPC分类号： C12N9/1044 , C12Y203/02013
摘要： A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).
摘要翻译： 突变以具有改善的耐热性和/或pH稳定性的转谷氨酰胺酶蛋白。 在能够形成二硫键的半胱氨酸残基（SS键）上引入WT转谷氨酰胺酶的突变。
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43.发明授权Selective molecular excitation method and isotope separation method using the same, isotope analysis method, selective molecular excitation apparatus and isotope separation apparatus 有权选择性分子激发法和同位素分离法使用同位素分析方法，选择性分子激发装置和同位素分离装置公开(公告)号：US08247762B2
公开(公告)日：2012-08-21
申请号：US12923444
申请日：2010-09-22
申请人： Keiichi Yokoyama , Leo Matsuoka , Hiroshi Akagi , Tatsuya Kasajima , Masaaki Tsubouchi , Atsushi Yokoyama
发明人： Keiichi Yokoyama , Leo Matsuoka , Hiroshi Akagi , Tatsuya Kasajima , Masaaki Tsubouchi , Atsushi Yokoyama
IPC分类号： B01D59/00
CPC分类号： B01D59/34
摘要： Molecules of a specific species can be selectively excited among molecules of a plurality of species that show only a slight difference of mass. Energy levels can be displayed on a graph where the horizontal axis indicates excitation energy. Assume an instance where an electromagnetic wave showing a comb-shaped spectrum having a plurality of narrow bands as indicated by P1 through P14 and tuning with excitation energies corresponding to the rotational levels of molecule X is irradiated onto the molecule X. The molecule X can sequentially make transitions to higher energy levels by using an electromagnetic wave showing such a comb-shaped spectrum. The energy levels of molecule Y are not synchronized with the comb-shaped spectrum. The two ranges of Y4 through Y7 and Y12 through Y15 operate as gates and the molecule Y cannot make transition from a rotational level to another when a gate is found between them.
摘要翻译： 可以在仅显示轻微质量差异的多种物种的分子中选择性地激发特定物种的分子。 能量水平可以显示在横轴表示激发能量的图形上。 假设示出具有如P1至P14所示的多个窄带的梳状光谱的电磁波和对应于分子X的旋转电平的激发能量的调谐的情况。分子X可以顺序地 通过使用显示这样的梳状光谱的电磁波使过渡到更高的能级。 分子Y的能级与梳状光谱不同步。 Y4至Y7和Y12至Y15的两个范围作为门进行操作，并且当在它们之间发现栅极时，分子Y不能从旋转电平转变为另一个。
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公开(公告)号：US08005904B2
公开(公告)日：2011-08-23
申请号：US11917673
申请日：2006-06-29
申请人： Keiichi Yokoyama , Yasuhiko Mori , Makoto Fukuda , Yoshiaki Hara , Rajesh Ramanathan , Christopher C. Yu , Fumiaki Yagi
发明人： Keiichi Yokoyama , Yasuhiko Mori , Makoto Fukuda , Yoshiaki Hara , Rajesh Ramanathan , Christopher C. Yu , Fumiaki Yagi
IPC分类号： G06F15/173 , H04L29/06 , H04L9/32
CPC分类号： G06F21/6245 , G06Q10/10
摘要： To provide a user with better experience of cooperation between an electronic business card processing program and a communication program when exchanging electronic business cards. Electronic business cards are stored in a DB server, which is one of storages for a communication program, as well as an electronic business card local file. Thus, even if a PC is a company's PC, for example, the electronic business cards can be viewed by accessing the DB server from another PC or the like outside an office. Further, the electronic business card processing program operates with the communication program as backend, however, the electronic business card processing program may not be installed on a PC in some cases. Even in such a case, the communication program alone can display received electronic business cards that are stored in the communication program local file on the screen of a display.
摘要翻译： 为了在交换电子名片时为用户提供更好的电子名片处理程序与通信程序之间的合作经验。 电子名片存储在DB服务器中，DB服务器是用于通信程序的存储器之一，以及电子名片本地文件。 因此，即使PC是公司的PC，例如，可以通过从办公室外的其他PC等访问DB服务器来查看电子名片。 此外，电子名片处理程序以通信程序作为后端进行操作，但是在某些情况下，电子名片处理程序可能不会安装在PC上。 即使在这种情况下，仅通信程序也可以在显示器的屏幕上显示存储在通信程序本地文件中的所接收的电子名片。
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公开(公告)号：US07972829B2
公开(公告)日：2011-07-05
申请号：US12714151
申请日：2010-02-26
IPC分类号： C12N9/10 , C12N1/20 , C12N15/00 , C12P21/04 , A61K38/00 , C07H21/04 , C07H21/02 , C07K1/00 , C12Q1/68 , C12Q1/00
CPC分类号： C12N9/1044 , C07K2319/02 , C07K2319/036 , C12N9/52 , C12N15/625 , C12Y203/02013
摘要： The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium.According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).An intended foreign protein, in particular, transglutaminase, is produced by using an expression construct wherein the gene sequence of the intended foreign protein containing the pro-structure part, in particular, pro-transglutaminase gene sequence, is ligated to the downstream of a sequence encoding the signal peptide region from a coryneform bacterium, introducing this expressional genetic construct into a coryneform bacterium, culturing the thus transformed coryneform bacterium, and treating the extracellularly released protein with a protease, etc. to cleave and eliminate the pro-part.
摘要翻译： 本发明涉及通过棒状细菌分泌外源蛋白质，特别是转谷氨酰胺酶的方法。 根据本发明，提供了通过制备棒状细菌以产生工业上有用的外源蛋白质，特别是转谷氨酰胺酶并有效地将细胞外释放产物（即，通过转运谷氨酰胺酶）分泌外源蛋白质，特别是转谷氨酰胺酶的方法， 分泌生产）。 通过使用表达构建体产生预期的外源蛋白质，特别是转谷氨酰胺酶，其中含有前结构部分，特别是原转谷氨酰胺酶基因序列的预期外源蛋白的基因序列连接到序列的下游 编码来自棒状细菌的信号肽区域，将该表达遗传构建体引入棒状杆菌型细菌，培养由此转化的棒状杆菌型细菌，用蛋白酶等处理细胞外释放的蛋白质以切割和消除前体部分。
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公开(公告)号：US20110137007A1
公开(公告)日：2011-06-09
申请号：US12983500
申请日：2011-01-03
CPC分类号： C12P13/008 , C08G73/22 , C12N9/16 , C12N9/88
摘要： Provided is a method for efficiently producing a 3-amino-4-hydroxybenzoic acid-type compound by culturing a coryneform bacterium that has a gene encoding a mutated aspartokinase not subject to feedback inhibition, and that is transformed with a recombinant vector containing a DNA encoding a protein having an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde.
摘要翻译： 提供了通过培养具有编码不经反馈抑制的突变型天冬氨酸激酶的基因的棒状细菌，并且用含有编码的DNA的重组载体转化的有效产生3-氨基-4-羟基苯甲酸型化合物的方法 具有从磷酸二羟丙酮和天冬氨酸半醛形成3-氨基-4-羟基苯甲酸的活性的蛋白质。
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公开(公告)号：US20100297729A1
公开(公告)日：2010-11-25
申请号：US12714151
申请日：2010-02-26
CPC分类号： C12N9/1044 , C07K2319/02 , C07K2319/036 , C12N9/52 , C12N15/625 , C12Y203/02013
摘要： The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium.According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).An intended foreign protein, in particular, transglutaminase, is produced by using an expression construct wherein the gene sequence of the intended foreign protein containing the pro-structure part, in particular, pro-transglutaminase gene sequence, is ligated to the downstream of a sequence encoding the signal peptide region from a coryneform bacterium, introducing this expressional genetic construct into a coryneform bacterium, culturing the thus transformed coryneform bacterium, and treating the extracellularly released protein with a protease, etc. to cleave and eliminate the pro-part.
摘要翻译： 本发明涉及通过棒状细菌分泌外源蛋白质，特别是转谷氨酰胺酶的方法。 根据本发明，提供了通过制备棒状细菌以产生工业上有用的外源蛋白质，特别是转谷氨酰胺酶并有效地释放产物的方法（即，通过使细胞外有效释放外源蛋白质，特别是转谷氨酰胺酶） 分泌生产）。 通过使用表达构建体产生预期的外源蛋白质，特别是转谷氨酰胺酶，其中含有前结构部分，特别是原转谷氨酰胺酶基因序列的预期外源蛋白的基因序列连接到序列的下游 编码来自棒状细菌的信号肽区域，将该表达遗传构建体引入棒状杆菌型细菌，培养由此转化的棒状杆菌型细菌，用蛋白酶等处理细胞外释放的蛋白质以切割和消除前体部分。
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公开(公告)号：US07704707B2
公开(公告)日：2010-04-27
申请号：US11218780
申请日：2005-09-06
CPC分类号： C12N9/1044 , C12N9/6489
摘要： The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.
摘要翻译： 本发明提供来自放线菌的中性金属蛋白酶，其选择性地切割微生物转谷氨酰胺酶的前体结构部分和编码所述中性金属蛋白酶的基因。 具有前体结构部分切割的活性微生物转谷氨酰胺酶可以通过培养从引入放线菌中产生中性金属蛋白酶的本发明的编码中性金属蛋白酶的基因的微生物获得，其中通过从放线菌产生中性金属蛋白酶， 微生物抗转谷氨酰胺酶。
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公开(公告)号：US07553650B2
公开(公告)日：2009-06-30
申请号：US11505438
申请日：2006-08-17
申请人： Tatsuki Kashiwagi , Nobuhisa Shimba , Kohki Ishikawa , Ei-ichiro Suzuki , Keiichi Yokoyama , Kazuo Hirayama
发明人： Tatsuki Kashiwagi , Nobuhisa Shimba , Kohki Ishikawa , Ei-ichiro Suzuki , Keiichi Yokoyama , Kazuo Hirayama
CPC分类号： C12N9/1044 , C07K14/195 , C07K2299/00 , C12N15/102 , C12Y203/02013
摘要： The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.
摘要翻译： 本发明涉及基于来自链霉菌（MTG）的MTG的三维结构和由此制备的突变体MTG来设计和制备突变转谷氨酰胺酶的方法。 本发明提供了一种基于三维结构修饰MTG的方法，并且通过该方法改善了在基板上具有反应性的转谷氨酰胺酶。 在本发明中，基于通过MTG晶体的X射线晶体结构分析获得的三维结构外推基质的MTG的结合位点，通过置换，插入或缺失氨基酸来设计和生产突变型转谷氨酰胺酶 位于转谷氨酰胺酶底物结合位点的残基。
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公开(公告)号：US06833258B2
公开(公告)日：2004-12-21
申请号：US09892864
申请日：2001-06-28
申请人： Keiichi Yokoyama , Kunio Ono , Daisuke Ejima
发明人： Keiichi Yokoyama , Kunio Ono , Daisuke Ejima
IPC分类号： C12N910
CPC分类号： C12N9/1044 , C12Y203/02013
摘要： The present invention relates to a high-yield process for producing high-activity, high-purity transglutaminase by refolding denatured microorganism-derived transglutaminase by reacting denatured transglutaminase in an aqueous medium under acidic conditions and reconstituting a higher-order enzymatically active state in an aqueous medium at a neutral pH.
摘要翻译： 本发明涉及一种生产高活性，高纯度转谷氨酰胺酶的高产率方法，其通过在酸性条件下将变性转谷氨酰胺酶在水性介质中反应并重新折叠变性微生物转谷氨酰胺酶，并在水性中重新构建高级酶活性状态 培养基处于中性pH值。
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