摘要:
The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.
摘要:
The present invention relates to a method for designing and preparing mutant transglutaminases on the basis of the three-dimensional structure of MTG derived from Streptoverticillium mobaraense (MTG), and the mutant MTG thus prepared. The present invention provides a method for modifying MTG on the basis of the three-dimensional structure, and transglutaminase having reactivity on the substrate improved by the method. In the present invention, the binding site of MTG for the substrate is extrapolated based on the three-dimensional structure obtained by X-ray crystal structure analysis of MTG crystals, and the mutant transglutaminases are designed and produced by replacing, inserting or deleting amino acid residues positioned at the substrate-binding site of the transglutaminase.
摘要:
O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genus Escherichia which harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.
摘要翻译:O-乙酰丝氨酸,L-半胱氨酸和从其衍生的亚硫酸化合物可以使用属于埃希氏杆菌属的细菌产生,其含有突变反馈抗性丝氨酸乙酰转移酶,其中对应于野生型的89至96位的氨基酸序列 丝氨酸乙酰转移酶被SEQ ID NO:4至9所示的氨基酸序列中的任何一个替代,并且细菌中L-半胱氨酸的反馈抑制被脱敏。
摘要:
O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genus Escherichia which harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.
摘要翻译:O-乙酰丝氨酸,L-半胱氨酸和从其衍生的亚硫酸化合物可以使用属于埃希氏杆菌属的细菌产生,其含有突变反馈抗性丝氨酸乙酰转移酶,其中对应于野生型的89至96位的氨基酸序列 丝氨酸乙酰转移酶被SEQ ID NO:4至9所示的氨基酸序列中的任何一个替代,并且细菌中L-半胱氨酸的反馈抑制被脱敏。
摘要:
A D-aminotransferase can be modified so as to efficiently produce (2R, 4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R, 4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.
摘要翻译:可以修饰D-氨基转移酶,以便通过用氨基酸至少在4-(吲哚-3-基甲基)-4-羟基-2-氧代戊二酸代替氨基酸,有效地产生具有高甜度强度的(2R,4R) 在SEQ ID NO:2所示的野生型D-氨基转移酶的氨基酸序列中有效产生(2R,4R) - 单抗的位置(位置100,180〜183,243和244)之一。
摘要:
A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R,4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.
摘要翻译:可以修饰D-氨基转移酶,以便通过用氨基酸至少在4-(吲哚-3-基甲基)-4-羟基-2-氧代戊二酸代替氨基酸,有效地产生具有高甜度强度的(2R,4R) 在SEQ ID NO:2所示的野生型D-氨基转移酶的氨基酸序列中有效产生(2R,4R) - 单抗的位置(位置100,180〜183,243和244)之一。
摘要:
A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by mutating the amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:4.
摘要翻译:可以修饰D-氨基转移酶,以便通过将氨基酸序列的突变来有效地从4-(吲哚-3-基甲基)-4-羟基-2-氧代戊二酸生成具有高甜度强度的(2R,4R) SEQ ID NO:4所示的野生型D-氨基转移酶。
摘要:
A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R,4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.
摘要翻译:可以修饰D-氨基转移酶,以便通过用氨基酸至少在4-(吲哚-3-基甲基)-4-羟基-2-氧代戊二酸代替氨基酸,有效地产生具有高甜度强度的(2R,4R) 在SEQ ID NO:2所示的野生型D-氨基转移酶的氨基酸序列中有效产生(2R,4R) - 单抗的位置(位置100,180〜183,243和244)之一。
摘要:
A D-aminotransferase can be modified so as to efficiently produce (2R, 4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183,243 and 244) involved in efficiently producing the (2R, 4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.
摘要翻译:可以修饰D-氨基转移酶,以便通过用氨基酸至少在4-(吲哚-3-基甲基)-4-羟基-2-氧代戊二酸代替氨基酸,有效地产生具有高甜度强度的(2R,4R) 在SEQ ID NO:2中表示的野生型D-转氨酶的氨基酸序列中有效地产生(2R,4R) - 单抗的位置之一(位置100,180至183,243和244)。
摘要:
The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II). (I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2. (II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208
摘要翻译:本发明的目的在于提供优异的肽合成蛋白质和有效生产肽的方法。 在下列(I)和(II)所示的至少一种蛋白质存在下,使胺组分和羧基组分反应合成肽。 (I)具有包含SEQ ID NO:2的氨基酸序列中的任何突变1至68和突变239至290和324至377的一个或多个突变的氨基酸序列的突变蛋白。 (II)具有包含SEQ ID NO:208的氨基酸序列中的任何突变L1至L335和M1至M642的一个或多个突变的氨基酸序列的突变蛋白