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1.发明授权Process for efficiently producing transglutaminase through DNA recombination 失效通过DNA重组有效生产转谷氨酰胺酶的方法
公开(公告)号：US5827712A
公开(公告)日：1998-10-27
申请号：US649193
申请日：1996-05-17
CPC分类号： C12N9/1044 , C07K14/245 , C12N15/67 , Y10S530/825
摘要： The present invention relates to a process for producing a transglutaminase, which comprises incubating Escherichia coli expressing genes encoding a heat shock protein (DnaJ) and a transglutaminase. The transglutaminase is produced in large quantities, at low cost and has the appropriate stereostructure to render the transglutaminase biologically active. The transglutaminase so produced is useful in the food industry.
摘要翻译： 本发明涉及一种转谷氨酰胺酶的制备方法，其包括将表达编码热休克蛋白（DnaJ）和转谷氨酰胺酶的基因表达的大肠杆菌。 转谷氨酰胺酶以低成本大量生产并具有合适的立体结构以使转谷氨酰胺酶具有生物活性。 如此生产的转谷氨酰胺酶在食品工业中很有用。
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公开(公告)号：US07439038B2
公开(公告)日：2008-10-21
申请号：US10716480
申请日：2003-11-20
申请人： Yoshiya Gunji , Hisashi Yasueda
发明人： Yoshiya Gunji , Hisashi Yasueda
摘要： A DNA encoding for a mutant of LysE protein, or a homologous protein thereof, of a coryneform bacterium, wherein the mutant, when introduced into a methanol-assimilating bacterium imparts resistance to L-lysine analogue. The DNA encoding for a mutant of LysE protein, or a homologous protein thereof, is introduced into a methanol-assimilating bacterium to improve L-lysine and L-arginine productivity of the methanol-assimilating bacterium.
摘要翻译： 编码棒状细菌的LysE蛋白或其同源蛋白质的突变体的DNA，其中所述突变体在被引入甲醇同化细菌中时赋予对L-赖氨酸类似物的抗性。 将编码LysE蛋白或其同源蛋白质的突变体的DNA引入甲醇同化细菌中以改善甲醇同化细菌的L-赖氨酸和L-精氨酸生产率。
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公开(公告)号：US07217543B2
公开(公告)日：2007-05-15
申请号：US10716473
申请日：2003-11-20
申请人： Yoshiya Gunji , Hisashi Yasueda
发明人： Yoshiya Gunji , Hisashi Yasueda
IPC分类号： C12P13/04
摘要： The present invention describes a method for producing an L-amino acid comprising culturing a microorganism having an ability to produce an L-amino acid in a medium, whereby the L-amino acid accumulates in the medium, and collecting the L-amino acid from the medium, whereby said microorganism comprises a methanol-utilizing bacterium having the Entner-Doudoroff pathway in which 6-phosphogluconate dehydratase activity and/or 2-keto-3-deoxy-6-phosphogluconate aldolase activity is enhanced.
摘要翻译： 本发明描述了L-氨基酸的制造方法，其特征在于，在培养基中培养具有产生L-氨基酸的能力的微生物，由此L-氨基酸积累在培养基中，从L- 所述微生物包含具有Ent-Doudoroff途径的甲醇利用细菌，其中6-磷酸葡萄糖酸脱水酶活性和/或2-酮-3-脱氧-6-磷酸葡糖酸醛缩酶活性增强。
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4.发明授权Method for producing L-lysine or L-arginine by using methanol assimilating bacterium 有权通过使用甲醇同化细菌生产L-赖氨酸或L-精氨酸的方法公开(公告)号：US07169587B2
公开(公告)日：2007-01-30
申请号：US10166142
申请日：2002-06-11
申请人： Yoshiya Gunji , Hisashi Yasueda
发明人： Yoshiya Gunji , Hisashi Yasueda
CPC分类号： C07K14/195
摘要： A DNA encoding a variant of a protein, having a loop region and six hydrophobic helixes and involved in excretion of L-lysine to outside of a cell, wherein the DNA encodes a mutant protein not containing the loop region that is contained in a wild-type protein and facilitates excretion of L-lysine, L-arginine or both of these L-amino acids to outside of a cell of a methanol assimilating bacterium when the DNA is introduced into the bacterium, specifically lysE24, is introduced into a methanol assimilating bacterium such as Methylophilus bacteria to improve L-amino acid productivity, especially L-lysine and L-arginine productivities.
摘要翻译： 编码蛋白质变体的DNA，具有环状区域和6个疏水性螺旋并涉及将L-赖氨酸排泄至细胞外，其中所述DNA编码不含有野生型的环状区域的突变型蛋白质， 当将DNA引入细菌中时，促进L-赖氨酸，L-精氨酸或这两种L-氨基酸的排泄到甲醇同化细菌的细胞外，特别是lysE24引入甲醇同化细菌 如甲状腺杆菌，提高L-氨基酸的生产力，特别是L-赖氨酸和L-精氨酸的生产力。
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公开(公告)号：US07163810B2
公开(公告)日：2007-01-16
申请号：US10234329
申请日：2002-09-05
申请人： Hisashi Yasueda , Ryo Takeshita
发明人： Hisashi Yasueda , Ryo Takeshita
CPC分类号： C12N9/0004 , C12P13/08
摘要： In a method for producing a target substance by using a microorganism comprising culturing a microorganism having an ability to produce the target substance in a medium to produce and accumulate the target substance in the medium or cells of the microorganism and collecting the target substance from the medium or the cells of the microorganism, there are used, as the microorganism, a microorganism to which a methanol dehydrogenase gene is introduced, of which activities of hexulose phosphate synthase and phosphohexuloisomerase are enhanced and which is modified so that an ability to utilize methanol should be imparted or enhanced, and there is used a medium containing methanol as a carbon source.
摘要翻译： 在通过使用微生物制造目标物质的方法中，包括在培养基中培养具有产生目标物质的能力的微生物，以在所述微生物的培养基或细胞中产生并积累所述目标物质并从所述培养基中收集所述目标物质 或微生物的细胞，使用引入了甲醇脱氢酶基因的微生物作为微生物，其中己糖磷酸合成酶和磷酸己糖异构酶的活性被增强，并且被修饰，使得使用甲醇的能力应该是 赋予或增强，并且使用含有甲醇作为碳源的介质。
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公开(公告)号：US07160704B2
公开(公告)日：2007-01-09
申请号：US10792647
申请日：2004-03-04
申请人： Ryo Takeshita , Hisashi Yasueda
发明人： Ryo Takeshita , Hisashi Yasueda
CPC分类号： C12N9/0006 , C12N9/1051 , C12N9/90 , C12P13/08
摘要： The present invention discloses a method for producing a target substance using a coryneform bacterium comprising culturing a coryneform bacterium having an ability to produce the target substance in a medium, resulting in accumulation of the target substance in the medium or cells of the bacterium, and collecting the target substance from the medium or the cells of the bacterium. Also disclosed is a coryneform bacterium which is introduced with a methanol dehydrogenase gene and which has enhanced activities of hexulose phosphate synthase and phosphohexuloisomerase, and to which an ability to utilize methanol is imparted or which has enhanced ability to utilize methanol, and the medium contains methanol as a carbon source.
摘要翻译： 本发明公开了一种使用棒状细菌生产目标物质的方法，包括培养在培养基中具有产生目标物质的能力的棒状细菌，导致目标物质在细菌的培养基或细胞中的积聚，并收集 来自培养基的目标物质或细菌的细胞。 还公开了一种引入了甲醇脱氢酶基因并且具有增强的己糖磷酸合成酶和磷酸己糖异构酶的活性的棒状细菌，并且其赋予使用甲醇的能力或具有增强的利用甲醇的能力，并且所述培养基含有甲醇 作为碳源。
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公开(公告)号：US20050176121A1
公开(公告)日：2005-08-11
申请号：US10791853
申请日：2004-03-04
申请人： Ryo Takeshita , Hisashi Yasueda , Yoshiya Gunji
发明人： Ryo Takeshita , Hisashi Yasueda , Yoshiya Gunji
CPC分类号： C12P7/04
摘要： The present invention describes a recombinant of a microorganism that does not inherently utilize an alkane, and an alcohol, whereby the recombinant has acquired an ability to convert the alkane into the alcohol due to transformation with a DNA encoding a methane oxygenase. The present invention describes a method for producing alcohol by culturing the recombinant, and allowing the obtained culture, cells isolated from the culture or processed product of the cells to exist with the alkane to produce the alcohol.
摘要翻译： 本发明描述了一种不固有地利用烷烃和醇的微生物重组体，由此由于用编码甲烷加氧酶的DNA转化，重组体已经获得了将烷烃转化为醇的能力。 本发明描述了通过培养重组体来制备醇的方法，并且使所得培养物从细胞的培养物或加工产物中分离的细胞与烷烃一起存在以产生醇。
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8.发明申请Method for producing L-lysine or L-arginine by using methanol-assimilating bacterium 失效使用甲醇同化细菌生产L-赖氨酸或L-精氨酸的方法公开(公告)号：US20050003495A1
公开(公告)日：2005-01-06
申请号：US10716470
申请日：2003-11-20
申请人： Yoshiya Gunji , Hisashi Yasueda
发明人： Yoshiya Gunji , Hisashi Yasueda
摘要： A DNA encoding a variant of a protein, the protein having a loop region and six hydrophobic helixes and involved in secretion of L-lysine to the outside of a cell, wherein the DNA encodes a variant of a protein not containing the loop region and facilitates secretion of L-lysine, L-arginine or both of these L-amino acids to the outside of a cell of a methanol-assimilating bacterium when the DNA is introduced into the bacterium, specifically lysE24, is introduced into a Methylobacillus bacteria to improve L-amino acid productivity, especially L-lysine and L-arginine productivities.
摘要翻译： 编码蛋白质变体的DNA，具有环状区域的蛋白质和六个疏水性螺旋并涉及将L-赖氨酸分泌到细胞外，其中所述DNA编码不含环状区域的蛋白质的变体，并且促进 将DNA引入细菌（特别是lysE24）中时，将L-赖氨酸，L-精氨酸或这两种L-氨基酸分泌到甲醇同化细菌的细胞外部，引入到甲基芽孢杆菌属细菌中以改善L - 氨基酸生产率，特别是L-赖氨酸和L-精氨酸生产力。
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9.发明申请MICROORGANISM PRODUCING AN AMINO ACID OF THE L-GLUTAMIC ACID FAMILY AND A METHOD FOR PRODUCING THE AMINO ACID 有权生产L-谷氨酸家族的氨基酸的微生物和生产氨基酸的方法公开(公告)号：US20100062497A1
公开(公告)日：2010-03-11
申请号：US12555124
申请日：2009-09-08
申请人： Seizaburo Shiraga , Noriko Murayama , Hiroshi Izui , Hisao Ito , Hisashi Yasueda , Yoshihiro Usuda , Kazuhiko Matsui
发明人： Seizaburo Shiraga , Noriko Murayama , Hiroshi Izui , Hisao Ito , Hisashi Yasueda , Yoshihiro Usuda , Kazuhiko Matsui
CPC分类号： C12P13/14 , C12N9/0008 , C12P13/10 , C12P13/24
摘要： A microorganism is cultured in a medium, and is able to produce one or two or more kinds of L-amino acids including L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline and L-arginine, and is modified to increase α-ketoglutarate synthase activity. The L-amino acids are collected from the medium or the cells.
摘要翻译： 在培养基中培养微生物，能够生成L-谷氨酸，L-谷氨酰胺，L-脯氨酸，L-鸟氨酸，L-瓜氨酸和L-精氨酸等一种或两种以上的L-氨基酸， 并被修饰以增加α-酮戊二酸合酶活性。 从培养基或细胞收集L-氨基酸。
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10.发明授权公开(公告)号：US07211421B2
公开(公告)日：2007-05-01
申请号：US11073741
申请日：2005-03-08
摘要： An Escherichia coli mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed with a chromosomal gene library of Bacillus methanolicus, and a transformant strain which can grow on a minimal medium is selected. Recombinant DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase (named dapB) is obtained from the transformant.
摘要翻译： 用甲醇梭菌的染色体基因文库转化二氢吡啶二羧酸合酶或二氢吡啶二羧酸还原酶缺陷的大肠杆菌突变菌株，选择能在最小培养基上生长的转化株。 从转化体获得编码二氢吡啶二酸合酶或二氢吡啶二羧酸还原酶（命名为dapB）的重组DNA。
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