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公开(公告)号：US5403714A
公开(公告)日：1995-04-04
申请号：US5086
申请日：1993-01-15
IPC分类号： G01N33/569 , G01N33/574 , G01N33/536 , G01N33/558
CPC分类号： G01N33/569 , G01N33/56966 , G01N33/574 , Y10S436/80 , Y10S436/805 , Y10S436/81 , Y10S436/813 , Y10S436/824
摘要： A biologic sample such as feces, sputum, cervical tissue, pleural fluids, exudates, cytologic specimens, or the like, is tested for the presence or absence of: ova; parasites; microorganisms; inflammatory, neoplastic tissue cells; or other target materials which are indicative of infestation, disease or infection. The sample is mixed with a buffer fluid and placed in a transparent tube which contains a volume-constricting cylindrical insert for gravimetric separation of components of the sample. The mixture is centrifuged, and the annular space between the insert and tube bore is examined under magnification for the presence of the target materials.
摘要翻译： 对生物样品如粪便，痰液，宫颈组织，胸膜液，渗出液，细胞学标本等进行测试，以确定是否存在：ova; 寄生虫 微生物 炎性，肿瘤组织细胞; 或指示感染，疾病或感染的其他目标材料。 将样品与缓冲液混合并置于含有体积收缩的圆柱形插入物的透明管中，用于重量分离样品组分。 将混合物离心，并且在放大下检查插入件和管孔之间的环形空间以存在目标材料。
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公开(公告)号：US5132087A
公开(公告)日：1992-07-21
申请号：US421639
申请日：1989-10-16
CPC分类号： B01L3/5021 , G01N15/05 , G01N33/491
摘要： Blood constituents are separated by centrifugation in a transparent tube which contains a float for physically elongating certain of the constituents. The centrifuged sample in the tube is positioned on a layer measuring device with which red cell and buffy coat constituent band heights can be measured in ambient light. The buffy coat constituent measurements are made under optical magnification. The measuring device has a mathematically derived red cell layer nomogram printed thereon, and a separate scale for measuring buffy coat constituent bands. Conversion tables are provided for converting the measured buffy coat constituent band lengths into constituent cell counts. The measuring device includes tube-engaging portions for properly positioning the tube during the measurements, and the red cell scale automatically compensates for the presence of the float and densification of red cells needed for proper banding.
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公开(公告)号：US4952054A
公开(公告)日：1990-08-28
申请号：US303120
申请日：1989-01-30
IPC分类号： G01N15/05
CPC分类号： G01N15/05
摘要： The method of correcting readings of cell counts in a centrifuged blood sample contained in a transparent tube which also contains a transparent cylindrical float. Light absorption measurements are made at different locations in the tube. A beam of light is directed through the tube and the float and a light absorption reading is taken. A light beam is also directed through the sample above the float and another absorption reading is taken. The ratio of the two readings is determined and compared to a target ratio that is precalculated from an ideal tube and float. Any deviations from the target ratio are proportional to correction factors which are applied to the cell readings taken. A colorant is added to the sample to impart the light absorption capability thereto.
摘要翻译： 在包含透明圆柱形浮子的透明管中的离心血样中校正细胞计数读数的方法。 光吸收测量在管中的不同位置进行。 光束被引导通过管和浮子并获得光吸收读数。 光束也被引导通过浮子上方的样品，并且进行另一吸收读数。 确定两个读数的比例，并与从理想管和浮子预先计算的目标比率进行比较。 与目标比率的任何偏差都与校正因子成比例，这些校正因子应用于所采集的细胞读数。 向样品中加入着色剂以赋予其光吸收能力。
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公开(公告)号：US4875364A
公开(公告)日：1989-10-24
申请号：US333265
申请日：1989-04-05
CPC分类号： B01L3/50215 , G01N33/491 , G01N9/12
摘要： A whole blood sample is placed in a tube such as a capillary tube, with a plastic float. The float is axially elongated and has a specific gravity which causes it to float in the packed red blood cells when the sample is centrifuged in the tube. The hemoglobin concentration of the packed red blood cells is measured by measuring the depth that the float sinks into the red cell layer, and then the hemoglobin concentration of the blood is calculated. The hemoglobin can be measured in this manner because virtually the only red cell component which contributes density to the red cells is the hemoglobin. The hemoglobin concentration of the whole blood is calculated by multiplying the mean corpuscular hemoglobin concentration of the packed red blood cells by the packed cell volume % (hematocrit) of the whole blood. All of the necessary calculations may be performed by a preprogrammed microprocessor.
摘要翻译： 将全血样品放置在具有塑料浮子的诸如毛细管的管中。 浮子轴向伸长并具有比重，当样品在管中离心时，该比例使其浮动在填充的红细胞中。 通过测量浮子沉入红细胞层的深度来测量填充的红细胞的血红蛋白浓度，然后计算血液的血红蛋白浓度。 可以以这种方式测量血红蛋白，因为实际上仅对红细胞贡献密度的红细胞成分是血红蛋白。 通过将填充的红细胞的平均红细胞血红蛋白浓度乘以全血的填充细胞体积％（血细胞比容）来计算全血的血红蛋白浓度。 所有必要的计算都可以由预编程的微处理器执行。
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45.发明授权Method of enhancing separation of abnormally light red cells from granulocytes in a centrifuged blood sample 失效在离心血液样品中增强异常轻红细胞与粒细胞分离的方法
公开(公告)号：US4594165A
公开(公告)日：1986-06-10
申请号：US671934
申请日：1984-11-16
CPC分类号： G01N33/491
摘要： In order to provide for a sharply defined line of demarcation between red cells and granulocytes in a centrifuged blood sample, the blood sample is drawn into a capillary tube. The bottom of the capillary tube is capped and the blood sample is centrifuged for a time sufficient to layer the blood sample in its constituent blood cell types. The capillary tube is then removed from the centrifuge and the top of the capillary tube is capped. The tube is then placed in the centrifuge inverted from its original orientation whereupon the sample is centrifuged again. Abnormally light red cells are thus trapped towards the bottom of the red cell column whereupon they cannot float up into the granulocyte band.
摘要翻译： 为了在离心的血液样品中提供红细胞和粒细胞之间明确界定的界线，将血液样品吸入毛细管。 将毛细管的底部封盖，并将血液样品离心一段足以使血液样品以其组成型血细胞类型分层的时间。 然后将毛细管从离心机中取出，并将毛细管的顶部盖上。 然后将管放置在从其原始方向反转的离心机中，然后将样品再次离心。 因此，红细胞异常被捕获到红细胞柱的底部，因此它们不能漂浮到粒细胞带中。
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公开(公告)号：US4567754A
公开(公告)日：1986-02-04
申请号：US717361
申请日：1985-03-29
CPC分类号： G01N15/05 , B01L3/50215
摘要： A complex liquid, such as a biological liquid, is stratified, as by centrifugation in a tube, into constituent layers. The most dense or heaviest constituent layer is measured volumetrically by physically expanding the entire layer with a cylindrical float which is inserted in the tube and which occupies the portion of the tube also occupied by the constituent layer being measured. The axial extent of the expanded layer is measured to provide an indication of the volume of the layer being measured. The end of the tube proximate to the layer being measured contains a plug of a more dense material which is immiscible with the subject liquid and into which the float will settle during centrifugation. The float thus settles into the plug of dense material and "floats" therein so as to extend through the entirety of the layer being measured.
摘要翻译： 诸如生物液体的复杂液体通过在管中离心而分层成组成层。 通过用插入管中的圆柱形浮子物理地扩展整个层并且占据被测量的构成层占据管的部分的体积来测量最密集或最重的构成层。 测量膨胀层的轴向范围以提供被测量层的体积的指示。 靠近所测量的层的管的端部包含与主体液体不混溶的更致密材料的塞子，并且浮子将在离心期间沉降到该塞子中。 因此，浮子沉积在致密材料的塞子中并在其中“漂浮”，以便延伸穿过被测量的整个层。
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公开(公告)号：US4190328A
公开(公告)日：1980-02-26
申请号：US965591
申请日：1978-12-01
IPC分类号： C12Q1/04 , G01N33/487
CPC分类号： C12Q1/04 , Y10S359/90 , Y10S436/80 , Y10S436/811
摘要： A process for detecting parasites which are found in the blood of a host. A sample of blood is drawn into a capillary tube which contains a generally cylindrical mass having a specific gravity such that it will float in one of the cell layers when the sample is separated by centrifugation. The specific gravity of the mass is selected so as to cause the cylindrical mass to combine with the bore wall of the capillary tube to form a thin annular space in the capillary tube into which the parasites will be crowded, thus increasing the concentration of parasites in a restricted area of the centrifuged blood sample and rendering the parasites highly visible. A stain may be used to differentially color the parasites. One specific parasite which can be detected in this manner quickly and inexpensively is heartworm.
摘要翻译： 用于检测在宿主血液中发现的寄生虫的过程。 将血液样品吸入毛细管中，该毛细管含有通常为圆柱形的质量，其具有比重，使得当样品通过离心分离时，其将漂浮在细胞层之一中。 选择质量的比重以使圆柱形物质与毛细管的孔壁结合，以在毛细管中形成薄的环形空间，寄生虫将被挤入其中，从而增加寄生虫的浓度 离心血液样本的限制区域并使寄生虫高度可见。 可以使用污渍来差别地染色寄生虫。 一种可以以这种方式迅速而廉价地检测到的特定寄生虫是心丝虫。
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48.发明授权Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching 有权使用荧光猝灭和/或漂白分析单个细胞或微粒的方法和装置公开(公告)号：US08994930B2
公开(公告)日：2015-03-31
申请号：US13959294
申请日：2013-08-05
CPC分类号： G01N33/49 , G01N15/1463 , G01N15/1475 , G01N21/6428 , G01N21/6456 , G01N33/48 , G01N33/5091 , G01N2015/008 , G01N2015/1477 , G01N2021/6432 , G01N2021/6439 , G01N2201/062
摘要： A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.
摘要翻译： 提供了一种用于分析血液样本的方法，其包括以下步骤：提供具有每个第一和第二成分中的一个或多个的血液样品; 将着色剂与样品混合，该着色剂可操作以使第一组分和第二组分发光和吸收光; 照亮样品的至少一部分; e）对样品的一部分进行成像; 确定每个第一组分和第二组分的荧光值; 确定每个第一组分和第二组分的光密度值; 以及使用所确定的荧光和光密度值来识别第一成分和第二成分。
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49.发明授权Method for serologic agglutination and other immunoassays performed in a thin film fluid sample 有权在薄膜液体样品中进行血清学凝集和其他免疫测定的方法公开(公告)号：US08569076B2
公开(公告)日：2013-10-29
申请号：US12417353
申请日：2009-04-02
IPC分类号： G01N33/543
CPC分类号： G01N33/48 , G01N21/6428 , G01N33/5304 , G01N33/54373 , G01N33/54386
摘要： A method and system for performing a serological agglutination assay in a liquid sample. The system provides a simple method for creating an in-situ sample/reagent admixture within a sample analysis chamber without the use of any precision fluid-handling components.
摘要翻译： 用于在液体样品中进行血清学凝集测定的方法和系统。 该系统提供了在样品分析室内创建原位样品/试剂外加剂的简单方法，而不使用任何精密的流体处理组件。
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50.发明授权Virtual separation of bound and free label in a ligand assay for performing immunoassays of biological fluids, including whole blood 有权用于进行生物液体包括全血的免疫测定的配体测定中的结合和游离标记的虚拟分离公开(公告)号：US08319954B2
公开(公告)日：2012-11-27
申请号：US13204416
申请日：2011-08-05
IPC分类号： G01N33/48
CPC分类号： G01N33/48 , G01N21/6428 , G01N33/5304 , G01N33/54373 , G01N33/54386
摘要： Detection and characterization of immunologically detected substances are performed electronically on human and animal biological fluids such as whole blood, serum, plasma, urine, milk, pleural and peritoneal fluids, and semen, which fluids are contained in a thin chamber forming a quiescent fluid sample, which chamber has at least two parallel planar walls, at least one of which is transparent.
摘要翻译： 免疫检测物质的检测和表征以人和动物生物流体如全血，血清，血浆，尿，牛奶，胸膜和腹膜液以及精液电子方式进行，这些流体包含在形成静态流体样品的薄室中 该室具有至少两个平行的平面壁，其中至少一个是透明的。
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