公开(公告)号：US20150097939A9

公开(公告)日：2015-04-09

申请号：US13919666

申请日：2013-06-17

申请人： Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

发明人： Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

IPC分类号： G02B21/36

CPC分类号： G02B21/365 ,  G02B21/244

摘要： A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect includes the steps of: disposing lenslets within a biologic sample, which lenslets have a height and a refractive index, which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; imaging a portion of the sample including lenslets using transmittance at one or more wavelengths; determining an average light transmittance intensity of the sample at the wavelengths; determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.

摘要翻译： 提供了用于聚焦用于对生物样品进行成像的装置的方法和装置。 方法方面包括以下步骤：在生物样品中设置小透镜，哪些小透镜具有高度和折射率，其折射率与样品的折射率不同，其中成像装置和样品中的一个或两个可相对定位 因此成像装置的焦点位置可以沿着小透镜的高度移动; 使用一个或多个波长的透射率成像包括小透镜的样品的一部分; 确定所述样品在所述波长处的平均光透射强度; 确定每个小透镜在该波长处的区域的平均光透射强度; 以及使用样品的平均光透射强度和小透镜区域的平均透光强度来确定成像装置的焦点位置。

公开(公告)号：US08994930B2

公开(公告)日：2015-03-31

申请号：US13959294

申请日：2013-08-05

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N15/14 ,  G01N21/64 ,  G01N15/00

CPC分类号： G01N33/49 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/48 ,  G01N33/5091 ,  G01N2015/008 ,  G01N2015/1477 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2201/062

摘要： A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.

摘要翻译： 提供了一种用于分析血液样本的方法，其包括以下步骤：提供具有每个第一和第二成分中的一个或多个的血液样品; 将着色剂与样品混合，该着色剂可操作以使第一组分和第二组分发光和吸收光; 照亮样品的至少一部分; e）对样品的一部分进行成像; 确定每个第一组分和第二组分的荧光值; 确定每个第一组分和第二组分的光密度值; 以及使用所确定的荧光和光密度值来识别第一成分和第二成分。

公开(公告)号：US20140368631A1

公开(公告)日：2014-12-18

申请号：US13919666

申请日：2013-06-17

申请人： Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

发明人： Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

IPC分类号： G02B21/36

CPC分类号： G02B21/365 ,  G02B21/244

摘要： A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect includes the steps of: disposing lenslets within a biologic sample, which lenslets have a height and a refractive index, which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; imaging a portion of the sample including lenslets using transmittance at one or more wavelengths; determining an average light transmittance intensity of the sample at the wavelengths; determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.

摘要翻译： 提供了用于聚焦用于对生物样品进行成像的装置的方法和装置。 方法方面包括以下步骤：在生物样品中设置小透镜，哪些小透镜具有高度和折射率，其折射率与样品的折射率不同，其中成像装置和样品中的一个或两个可相对定位 因此成像装置的焦点位置可以沿着小透镜的高度移动; 使用一个或多个波长的透射率成像包括小透镜的样品的一部分; 确定所述样品在所述波长处的平均光透射强度; 确定每个小透镜在该波长处的区域的平均光透射强度; 以及使用样品的平均光透射强度和小透镜区域的平均透光强度来确定成像装置的焦点位置。

公开(公告)号：US08753890B2

公开(公告)日：2014-06-17

申请号：US13314959

申请日：2011-12-08

申请人： Niten V. Lalpuria ,  Darryn W. Unfricht ,  Igor Nikonorov ,  Benjamin Ports ,  Douglas R. Olson

发明人： Niten V. Lalpuria ,  Darryn W. Unfricht ,  Igor Nikonorov ,  Benjamin Ports ,  Douglas R. Olson

IPC分类号： G01N33/48 ,  G01N21/05 ,  B01L3/00 ,  G01N33/487 ,  G01N21/03 ,  G01N15/14

CPC分类号： B01L3/5027 ,  B01F11/0071 ,  B01F13/0059 ,  B01L3/502707 ,  B01L2200/14 ,  B01L2300/0816 ,  B01L2300/161 ,  B01L2300/165 ,  G01N15/1463 ,  G01N15/1484 ,  G01N21/0303 ,  G01N21/05 ,  G01N33/48 ,  G01N33/487 ,  Y10T436/2575

摘要： An apparatus and method for analyzing a biological fluid sample is provided. The method includes the steps of: a) providing an analysis cartridge having a channel and an analysis chamber, wherein the channel is in fluid communication with the analysis chamber and includes at least one hydrophobic interior wall surface; b) admixing one or more anti-adsorption agents with fluid sample disposed within the channel, wherein the anti-adsorption agents are operable to inhibit adsorption of fluid sample onto the interior wall surface of the channel; c) moving the fluid sample into the analysis chamber; and d) analyzing the sample within the analysis chamber.

摘要翻译： 提供了用于分析生物流体样品的装置和方法。 该方法包括以下步骤：a）提供具有通道和分析室的分析盒，其中所述通道与分析室流体连通并且包括至少一个疏水性内壁表面; b）将一种或多种抗吸附剂与设置在通道内的流体样品混合，其中所述抗吸附剂可操作以抑制流体样品吸附到通道的内壁表面上; c）将流体样品移入分析室; 和d）分析分析室内的样品。

公开(公告)号：US20120195489A1

公开(公告)日：2012-08-02

申请号：US13418007

申请日：2012-03-12

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

IPC分类号： G06K9/46

CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

摘要： A method and apparatus for determining the hematocrit of a blood sample disposed within an analysis chamber, the method comprising the steps of: a) imaging at least a portion of the sample that contains one or more red blood cells contacting the interior surfaces of the chamber and one or more areas void of red blood cells; b) determining a representative optical density value for a plurality of image units optically aligned with portions of the red blood cells that are in contact the interior surfaces, and assigning an optical density first boundary value to those image units; c) determining a representative optical density value of a plurality of image units optically aligned with the one or more regions of the sample devoid of red blood cells, and assigning a second optical density boundary value to those image units; and d) determining the hematocrit of the sample.

摘要翻译： 一种用于确定设置在分析室内的血液样本的血细胞比容的方法和装置，所述方法包括以下步骤：a）成像至少一部分所述样品，所述样品包含接触所述腔室内表面的一个或多个红细胞 和一个或多个无红细胞的区域; b）确定与内表面接触的红细胞的光学对准的多个图像单元的代表性光密度值，以及向这些图像单元分配光密度第一边界值; c）确定与没有红细胞的样品的一个或多个区域光学对准的多个图像单元的代表性光密度值，以及向所述图像单元分配第二光密度边界值; 和d）确定样品的血细胞比容。

公开(公告)号：US08045165B2

公开(公告)日：2011-10-25

申请号：US12408479

申请日：2009-03-20

申请人： Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

发明人： Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

IPC分类号： G01N21/00

CPC分类号： G02B21/241

摘要： A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect of the disclosure includes the steps of: 1) disposing lenslets within a field of a biologic sample, which lenslets have a height, and have a refractive index and which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; 2) imaging at least a portion of the sample including a plurality of lenslets using transmittance at one or more predetermined wavelengths; 3) determining an average light transmittance intensity of the sample at the wavelengths; 4) determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and 5) determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.

摘要翻译： 提供了用于聚焦用于对生物样品进行成像的装置的方法和装置。 本公开的方法方面包括以下步骤：1）在生物样品的场内设置小透镜，哪些小透镜具有高度，并且具有折射率并且折射率不同于样品的折射率，其中一个或两个 成像装置和样品相对定位，使得成像装置的焦点位置可以沿着小透镜的高度移动; 2）使用一个或多个预定波长的透射率对包含多个小透镜的样品的至少一部分进行成像; 3）确定样品在波长处的平均光透射强度; 4）确定每个小透镜在该波长处的区域的平均透光强度; 以及5）使用样品的平均光透射强度和小透镜区域的平均光透射强度来确定成像装置的焦点位置。

公开(公告)号：US20090239257A1

公开(公告)日：2009-09-24

申请号：US12408448

申请日：2009-03-20

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

IPC分类号： C12Q1/04 ,  C12M1/34

CPC分类号： G01N33/49 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/48 ,  G01N33/5091 ,  G01N2015/008 ,  G01N2015/1477 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2201/062

摘要： A method for analyzing a blood sample is provided that includes the steps of: a) providing a blood sample having one or more first constituents and one or more second constituents, which second constituents are different from the first constituents; b) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; c) admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce upon exposure to predetermined first wavelengths of light, and which colorant is operative to absorb light at one or more predetermined second wavelengths of light; d) illuminating at least a portion of the sample containing the first constituents and the second constituents at the first wavelengths and at the second wavelengths; e) imaging the at least a portion of the sample, including producing image signals indicative of fluorescent emissions from the first constituents and the second constituents and the optical density of the first constituents and the second constituents; f) determining a fluorescence value for each the first constituents and second constituents using the image signals; g) determining an optical density value for each of the first constituents and second constituents, which optical density is a function of the colorant absorbed by the constituents, using the image signals; and h) identifying the first constituents and the second constituents using the determined fluorescence and optical density values.

摘要翻译： 提供了一种用于分析血液样本的方法，其包括以下步骤：a）提供具有一个或多个第一成分和一个或多个第二成分的血液样品，所述第二成分与第一成分不同; b）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板和第二面板限定，两个面板都是透明的; c）将着色剂与所述样品混合，所述着色剂可操作以使得所述第一组分和第二组分在暴露于预定的第一波长的光时发荧光，并且所述着色剂可操作地吸收一个或多个预定的第二波长的光的光; d）以第一波长和第二波长照射含有第一组分和第二组分的样品的至少一部分; e）对样品的至少一部分进行成像，包括产生指示来自第一组分和第二组分的荧光发射的图像信号和第一组分和第二组分的光密度; f）使用图像信号确定每个第一成分和第二成分的荧光值; g）使用所述图像信号确定所述第一成分和第二成分中的每一个的光密度值，所述光密度值是由所述成分吸收的着色剂的函数; 和h）使用所确定的荧光和光密度值来识别第一组分和第二组分。

公开(公告)号：US09291617B2

公开(公告)日：2016-03-22

申请号：US14673294

申请日：2015-03-30

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N21/64

CPC分类号： G01N33/49 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/48 ,  G01N33/5091 ,  G01N2015/008 ,  G01N2015/1477 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2201/062

摘要： A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.

公开(公告)号：US08481282B2

公开(公告)日：2013-07-09

申请号：US13190242

申请日：2011-07-25

申请人： Robert A. Levine ,  Darryn W. Unfricht ,  Stephen C. Wardlaw ,  Benjamin Ports

发明人： Robert A. Levine ,  Darryn W. Unfricht ,  Stephen C. Wardlaw ,  Benjamin Ports

IPC分类号： C12Q1/04

CPC分类号： G01N21/23 ,  G01N21/6458

摘要： An apparatus and method for detecting the presence of anisotropic crystals within a biologic fluid sample is provided. The method includes the steps of: a) disposing the sample within a sample chamber in a sample layer having a height not greater than about fifteen microns (15μ); b) disposing the sample layer within the sample chamber between a polarizing filter and an analyzing filter; c) disposing the polarizing filter, sample chamber, and analyzing filter in a configuration relative to a light source such that polarized light passes through the sample, and subsequently impinges on the analyzing filter; and d) wherein polar orientations of the polarizing filter and the analyzing filter are such that the polarized light will not pass through the analyzing filter, and light passing through an anisotropic crystal disposed within the sample will pass through the analyzing filter and appear as a point of light.

摘要翻译： 提供了一种用于检测生物液体样品中各向异性晶体的存在的装置和方法。 该方法包括以下步骤：a）将样品置于具有不大于约15微米（15mu）的高度的样品层中的样品室内; b）将样品层置于样品室内，在偏振滤光片和分析滤光片之间; c）将偏振滤光器，样品室和分析滤光片相对于光源配置成使得偏振光通过样品，随后撞击在分析滤光片上; 和d）其中偏振滤光器和分析滤光器的极性取向使得偏振光不会通过分析滤光器，并且穿过设置在样品内的各向异性晶体的光将通过分析滤光器并显现为点 的光。

公开(公告)号：US08472693B2

公开(公告)日：2013-06-25

申请号：US13051705

申请日：2011-03-18

申请人： Graham Davis ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten Lalpuria ,  Paul G. Mitsis

发明人： Graham Davis ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten Lalpuria ,  Paul G. Mitsis

IPC分类号： G06K9/00

CPC分类号： G01N33/726 ,  G01N15/1475 ,  G01N2015/0073

摘要： A method and apparatus for determining at least one hemoglobin related parameter of a whole blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; b) imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; c) determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces; and d) determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.

摘要翻译： 提供了一种用于确定全血样品的至少一种血红蛋白相关参数的方法和装置。 该方法包括以下步骤：a）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板的内表面和第二面板的内表面限定，并且所述室具有 在所述面板的内表面之间延伸的高度，其中所述腔被配置为在进入所述腔室之后的预定量的时间内将所述样品的氧合状态增加到基本上氧合状态; b）对与内表面接触的至少一个红细胞进行成像，并产生图像信号; c）确定接触两个内表面的成像红细胞的至少一部分的光密度; 以及d）使用确定的光密度和氧合血红蛋白的摩尔消光系数来确定与内表面接触的红细胞的至少一种血红蛋白相关参数。