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    Method and apparatus for determining the hematocrit of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
    5.
    发明授权
    Method and apparatus for determining the hematocrit of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells 有权
    利用红细胞内含有的血红蛋白的内在色素沉淀来测定血液样品的血细胞比容的方法和装置

    公开(公告)号：US08133738B2

    公开(公告)日：2012-03-13

    申请号：US13116837

    申请日：2011-05-26

    申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn Unfricht ,  Niten V. Lalpuria

    发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn Unfricht ,  Niten V. Lalpuria

    IPC分类号： G01N33/48 ,  G01N33/86

    CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

    摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.

    摘要翻译： 提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到适于静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和 高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。

    METHOD AND APPARATUS FOR DETERMINING THE HEMATOCRIT OF A BLOOD SAMPLE UTILIZING THE INTRINSIC PIGMENTATION OF HEMOGLOBIN CONTAINED WITHIN THE RED BLOOD CELLS
    6.
    发明申请
    METHOD AND APPARATUS FOR DETERMINING THE HEMATOCRIT OF A BLOOD SAMPLE UTILIZING THE INTRINSIC PIGMENTATION OF HEMOGLOBIN CONTAINED WITHIN THE RED BLOOD CELLS 有权
    用于确定使用红细胞中含有的HEMOGLOBIN的内在色素的血液样品的HEMATOCRIT的方法和装置

    公开(公告)号：US20110230740A1

    公开(公告)日：2011-09-22

    申请号：US13116837

    申请日：2011-05-26

    申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

    发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

    IPC分类号： A61B5/1455

    CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

    摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.

    摘要翻译： 提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到适于静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和 高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。

    Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching
    9.
    发明授权
    Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching 有权
    使用荧光猝灭和/或漂白分析单个细胞或微粒的方法和装置

    公开(公告)号：US08269954B2

    公开(公告)日：2012-09-18

    申请号：US13330253

    申请日：2011-12-19

    申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

    发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

    IPC分类号： G01N33/48

    CPC分类号： G01N33/49 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/48 ,  G01N33/5091 ,  G01N2015/008 ,  G01N2015/1477 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2201/062

    摘要： A method for analyzing a blood sample residing within an analysis chamber, which sample has one or more first and second constituents, which constituents are different from one another. The method includes the steps of: a) imaging the sample containing the first and second constituents, which sample is mixed with a colorant that causes the first and second constituents to fluoresce upon exposure to light, and which colorant is also operative to absorb light, the imaging produces image signals representative of fluorescent emissions from the sample and optical density of the sample; b) determining one or more values representative of fluorescent emission from the first and second constituents; c) determining one or more values representative of optical density for the first and second constituents; and d) distinguishing the first constituents from the second constituents using the fluorescent emission representative values and the optical density representative values.

    摘要翻译： 一种用于分析驻留在分析室内的血液样本的方法，该样品具有一个或多个第一和第二组分，该组分彼此不同。 该方法包括以下步骤：a）对包含第一和第二成分的样品进行成像，该样品与着色剂混合，使得第一和第二组分在暴露于光时发荧光，并且哪种着色剂也可操作地吸收光， 成像产生代表样品的荧光发射和样品的光密度的图像信号; b）确定代表来自第一和第二组分的荧光发射的一个或多个值; c）确定表示第一和第二组分的光密度的一个或多个值; 以及d）使用荧光发射代表值和光密度代表值来区分第一组分与第二组分。