公开(公告)号：US09873118B2

公开(公告)日：2018-01-23

申请号：US13341618

申请日：2011-12-30

申请人： John A. Verrant ,  Niten V. Lalpuria ,  Igor Nikonorov ,  Darryn W. Unfricht ,  Benjamin Ports ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Robert Holt ,  Kyle Hukari

发明人： John A. Verrant ,  Niten V. Lalpuria ,  Igor Nikonorov ,  Darryn W. Unfricht ,  Benjamin Ports ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Robert Holt ,  Kyle Hukari

IPC分类号： A61J1/06 ,  B01L3/00

CPC分类号： B01L3/502707 ,  B01L3/5027 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0861

摘要： A biological fluid analysis cartridge is provided. In certain embodiments, the cartridge includes a base plate extending between a sample handling portion and an analysis chamber portion. A handling upper panel is attached to the base plate within the sample handling portion. A collection port is at least partially formed with the handling upper panel. An initial channel and a secondary channel are formed between the handling upper panel and the base plate. The collection port and initial and secondary channels are in fluid communication with one another. A chamber upper panel is attached to the base plate within the analysis chamber portion. At least one analysis chamber is formed between the chamber upper panel and the base plate. The secondary channel and the analysis chamber are in fluid communication with one another.

公开(公告)号：US20160320369A1

公开(公告)日：2016-11-03

申请号：US15207074

申请日：2016-07-11

申请人： Joshua D. Levine ,  Robert A. Levine ,  Stephen C. Wardlaw ,  Craig Stout ,  David A. Clipper

发明人： Joshua D. Levine ,  Robert A. Levine ,  Stephen C. Wardlaw ,  Craig Stout ,  David A. Clipper

IPC分类号： G01N33/49 ,  G06T7/00 ,  B01L3/00 ,  B01L9/06 ,  G01N15/04 ,  G01N15/05

CPC分类号： G01N33/491 ,  B01L3/50215 ,  B01L9/065 ,  B01L2300/027 ,  B01L2300/0609 ,  B01L2300/0654 ,  B01L2300/0838 ,  G01N15/042 ,  G01N15/05 ,  G01N2015/045 ,  G01N2800/22 ,  G06T7/0012 ,  G06T2207/30024

摘要： A method and device for analyzing a hematologic sample centrifuged within a capillary tube is provided. The device includes a tube holder, a sample imaging device, a processor, and a sample data display. The sample imaging device is operable to create a digital image of the sample within a region of the tube. The region is defined by substantially all of the radial width and axial length of the sample residing within the internal cavity of the tube in the region where the float resides after centrifugation. The sample imaging device is operable to produce signals representative of the image. The processor is adapted to produce information relating to bands of interest within the image based on the signals from the sample imaging device. The sample data display is adapted to display the results therefrom and/or a digital image of the sample within the region.

摘要翻译： 提供了用于分析在毛细管内离心的血液样品的方法和装置。 该装置包括管座，样品成像装置，处理器和样品数据显示器。 样品成​​像装置可操作以在管的区域内产生样品的数字图像。 该区域由离心后浮子所在的区域中位于管内腔中的样品的基本上所有的径向宽度和轴向长度限定。 样品成​​像装置可操作以产生表示图像的信号。 处理器适于基于来自样本成像装置的信号产生与图像内的感兴趣的频带相关的信息。 样本数据显示器适于在该区域内显示样本的结果和/或数字图像。

公开(公告)号：US08774487B2

公开(公告)日：2014-07-08

申请号：US13016392

申请日：2011-01-28

申请人： Joshua D. Levine ,  Robert A. Levine ,  Stephen C. Wardlaw ,  Craig Stout ,  David A. Clipper

发明人： Joshua D. Levine ,  Robert A. Levine ,  Stephen C. Wardlaw ,  Craig Stout ,  David A. Clipper

IPC分类号： G06K9/00

CPC分类号： G01N33/491 ,  B01L3/50215 ,  B01L9/065 ,  B01L2300/027 ,  B01L2300/0609 ,  B01L2300/0654 ,  B01L2300/0838 ,  G01N15/042 ,  G01N15/05 ,  G01N2015/045 ,  G01N2800/22 ,  G06T7/0012 ,  G06T2207/30024

摘要： An apparatus for and method of analyzing hematologic samples deposited within a capillary tube is provided. The method includes the steps of: a) imaging a region of sample centrifuged within a capillary tube using a first analysis device, which region is defined by substantially all of the radial width and axial length of the sample residing within the internal cavity of the tube where the float resides after centrifugation, and producing signals representative of the image; b) communicating the signals representative of the image to a second analysis device independent of, and remotely located from, the first analysis device; c) processing the signals representative of the image using the second analysis device and producing analysis data based on the signals; and d) displaying the image of the region of the sample using the second analysis device.

摘要翻译： 提供了一种用于分析沉积在毛细管内的血液样品的方法和方法。 该方法包括以下步骤：a）使用第一分析装置对在毛细管内离心的样品的区域进行成像，该区域由位于管内腔内的样品的基本上所有的径向宽度和轴向长度限定 离心后浮子位于其中，并产生代表图像的信号; b）将表示所述图像的信号传送到与所述第一分析装置无关并且远离所述第一分析装置的第二分析装置; c）使用第二分析装置处理表示图像的信号，并基于该信号产生分析数据; 以及d）使用所述第二分析装置显示所述样品的区域的图像。

公开(公告)号：US20130029373A1

公开(公告)日：2013-01-31

申请号：US13645075

申请日：2012-10-04

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Niten V. Lalpuria ,  Darryn W. Unfricht

IPC分类号： G01N21/64 ,  C12M1/34

CPC分类号： G01N33/49 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/48 ,  G01N33/5091 ,  G01N2015/008 ,  G01N2015/1477 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2201/062

摘要： A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values.

摘要翻译： 提供了一种用于分析血液样本的方法，其包括以下步骤：提供具有每个第一和第二成分中的一个或多个的血液样品; 将着色剂与样品混合，该着色剂可操作以使第一组分和第二组分发光和吸收光; 照亮样品的至少一部分; e）对样品的一部分进行成像; 确定每个第一组分和第二组分的荧光值; 确定每个第一组分和第二组分的光密度值; 以及使用所确定的荧光和光密度值来识别第一成分和第二成分。

公开(公告)号：US08133738B2

公开(公告)日：2012-03-13

申请号：US13116837

申请日：2011-05-26

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn Unfricht ,  Niten V. Lalpuria

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn Unfricht ,  Niten V. Lalpuria

IPC分类号： G01N33/48 ,  G01N33/86

CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.

摘要翻译： 提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到适于静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和 高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。

公开(公告)号：US20110256573A1

公开(公告)日：2011-10-20

申请号：US13051705

申请日：2011-03-18

申请人： Graham Davis ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten Lalpuria ,  Paul G. Mitsis

发明人： Graham Davis ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten Lalpuria ,  Paul G. Mitsis

IPC分类号： C12Q1/02 ,  C12M1/34

CPC分类号： G01N33/726 ,  G01N15/1475 ,  G01N2015/0073

摘要： A method and apparatus for determining at least one hemoglobin related parameter of a whole blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; b) imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; c) determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces; and d) determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.

摘要翻译： 提供了一种用于确定全血样品的至少一种血红蛋白相关参数的方法和装置。 该方法包括以下步骤：a）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板的内表面和第二面板的内表面限定，并且所述室具有 在所述面板的内表面之间延伸的高度，其中所述腔被配置为在进入所述腔室之后的预定量的时间内将所述样品的氧合状态增加到基本上氧合状态; b）对与内表面接触的至少一个红细胞进行成像，并产生图像信号; c）确定接触两个内表面的成像红细胞的至少一部分的光密度; 以及d）使用确定的光密度和氧合血红蛋白的摩尔消光系数来确定与内表面接触的红细胞的至少一种血红蛋白相关参数。

公开(公告)号：US20110230740A1

公开(公告)日：2011-09-22

申请号：US13116837

申请日：2011-05-26

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

IPC分类号： A61B5/1455

CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.

摘要翻译： 提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到适于静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和 高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。

公开(公告)号：US20110201045A1

公开(公告)日：2011-08-18

申请号：US13016347

申请日：2011-01-28

申请人： Joshua D. Levine ,  Robert A. Levine ,  Stephen C. Wardlaw ,  Craig Stout ,  David A. Clipper

发明人： Joshua D. Levine ,  Robert A. Levine ,  Stephen C. Wardlaw ,  Craig Stout ,  David A. Clipper

IPC分类号： C12Q1/02 ,  C12M1/34

CPC分类号： G01N33/491 ,  B01L3/50215 ,  B01L9/065 ,  B01L2300/027 ,  B01L2300/0609 ,  B01L2300/0654 ,  B01L2300/0838 ,  G01N15/042 ,  G01N15/05 ,  G01N2015/045 ,  G01N2800/22 ,  G06T7/0012 ,  G06T2207/30024

摘要： A method and device for analyzing a hematologic sample centrifuged within a capillary tube is provided. The device includes a tube holder, a sample imaging device, a processor, and a sample data display. The sample imaging device is operable to create a digital image of the sample within a region of the tube. The region is defined by substantially all of the radial width and axial length of the sample residing within the internal cavity of the tube in the region where the float resides after centrifugation. The sample imaging device is operable to produce signals representative of the image. The processor is adapted to produce information relating to bands of interest within the image based on the signals from the sample imaging device. The sample data display is adapted to display the results therefrom and/or a digital image of the sample within the region.

摘要翻译： 提供了用于分析在毛细管内离心的血液样品的方法和装置。 该装置包括管座，样品成像装置，处理器和样品数据显示器。 样品成​​像装置可操作以在管的区域内产生样品的数字图像。 该区域由离心后浮子所在的区域中位于管内腔中的样品的基本上所有的径向宽度和轴向长度限定。 样品成​​像装置可操作以产生表示图像的信号。 处理器适于基于来自样本成像装置的信号产生与图像内的感兴趣的频带相关的信息。 样本数据显示器适于在该区域内显示样本的结果和/或数字图像。

公开(公告)号：US20090251683A1

公开(公告)日：2009-10-08

申请号：US12417246

申请日：2009-04-02

申请人： Stephen C. Wardlaw ,  Robert A. Levine

发明人： Stephen C. Wardlaw ,  Robert A. Levine

IPC分类号： G01N33/48

CPC分类号： G01N33/48 ,  G01N21/6428 ,  G01N33/5304 ,  G01N33/54373 ,  G01N33/54386

摘要： Detection and characterization of immunologically detected substances are performed electronically on human and animal biological fluids such as whole blood, serum, plasma, urine, milk, pleural and peritoneal fluids, and semen, which fluids are contained in a thin chamber forming a quiescent fluid sample, which chamber has at least two parallel planar walls, at least one of which is transparent.

摘要翻译： 免疫检测物质的检测和表征以人和动物生物流体如全血，血清，血浆，尿，牛奶，胸膜和腹膜液以及精液电子方式进行，这些流体包含在形成静态流体样品的薄室中 该室具有至少两个平行的平面壁，其中至少一个是透明的。

公开(公告)号：US20090162940A1

公开(公告)日：2009-06-25

申请号：US11569640

申请日：2005-05-17

申请人： Stephen C. Wardlaw ,  Robert A. Levine

发明人： Stephen C. Wardlaw ,  Robert A. Levine

IPC分类号： G01N1/10 ,  B01L3/00

CPC分类号： B01L3/5021 ,  A61B5/150022 ,  A61B5/150213 ,  A61B5/150343 ,  A61B5/150351 ,  A61B5/150755 ,  B01L2300/0838 ,  B01L2400/0406 ,  Y10T436/2575

摘要： A method and apparatus for analyzing a fluid sample. More particularly, this relates to a specimen analysis tube (2) for containing the specimen (12) sample and to a method of drawing the specimen sample (12) into the tube (2) by capillary action. When the sample (12) is drawn into the tube (2), air is expelled from the tube (2) in the direction which is counter to the flow of the sample (12) into the tube (2). The tube (2) can be U-shaped or it can be linear and can be disposed in a closed-ended holder (22) that is operative to vent air from the tube (2) in a direction which is counter to the direction of flow of the sample into the tube (2).

摘要翻译： 一种用于分析流体样品的方法和装置。 更具体地，涉及用于容纳样本（12）样本的样本分析管（2）和通过毛细管作用将样本样品（12）拉入管（2）的方法。 当样品（12）被吸入管（2）中时，空气从与样品（12）的流动相反的方向从管（2）排出到管（2）中。 管（2）可以是U形的，或者它可以是线性的，并且可以设置在闭合端的保持器（22）中，该保持器可操作以沿着与管（2）的方向相反的方向从管（2）排出空气 样品流入管（2）。