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209 条记录 (耗时 0.03 秒)

    Process for detection of blood-borne parasites
    1.
    发明授权
    Process for detection of blood-borne parasites 失效
    检测血源性寄生虫的过程

    公开(公告)号：US4190328A

    公开(公告)日：1980-02-26

    申请号：US965591

    申请日：1978-12-01

    申请人： Robert A. Levine ,  James V. Massey, III ,  Stephen C. Wardlaw

    发明人： Robert A. Levine ,  James V. Massey, III ,  Stephen C. Wardlaw

    IPC分类号： C12Q1/04 ,  G01N33/487

    CPC分类号： C12Q1/04 ,  Y10S359/90 ,  Y10S436/80 ,  Y10S436/811

    摘要： A process for detecting parasites which are found in the blood of a host. A sample of blood is drawn into a capillary tube which contains a generally cylindrical mass having a specific gravity such that it will float in one of the cell layers when the sample is separated by centrifugation. The specific gravity of the mass is selected so as to cause the cylindrical mass to combine with the bore wall of the capillary tube to form a thin annular space in the capillary tube into which the parasites will be crowded, thus increasing the concentration of parasites in a restricted area of the centrifuged blood sample and rendering the parasites highly visible. A stain may be used to differentially color the parasites. One specific parasite which can be detected in this manner quickly and inexpensively is heartworm.

    摘要翻译： 用于检测在宿主血液中发现的寄生虫的过程。 将血液样品吸入毛细管中，该毛细管含有通常为圆柱形的质量，其具有比重，使得当样品通过离心分离时，其将漂浮在细胞层之一中。 选择质量的比重以使圆柱形物质与毛细管的孔壁结合，以在毛细管中形成薄的环形空间，寄生虫将被挤入其中，从而增加寄生虫的浓度 离心血液样本的限制区域并使寄生虫高度可见。 可以使用污渍来差别地染色寄生虫。 一种可以以这种方式迅速而廉价地检测到的特定寄生虫是心丝虫。

    Material layer volume determination
    3.
    发明授权
    Material layer volume determination 失效
    材料层体积确定

    公开(公告)号：US4137755A

    公开(公告)日：1979-02-06

    申请号：US862543

    申请日：1977-12-20

    申请人： Stephen C. Wardlaw ,  Robert A. Levine ,  James V. Massey, III

    发明人： Stephen C. Wardlaw ,  Robert A. Levine ,  James V. Massey, III

    IPC分类号： B01L3/14 ,  G01N11/00

    CPC分类号： B01L3/50215

    摘要： A technique for generally determining the volume of a layer of a constituent material in a centrifugally separated mixture of material. A centrifuge tube is used to hold the material mixture and an appropriately shaped body is disposed in the tube in the zone occupied by the constituent material whose volume is to be measured. The body reduces the available volume within the tube which may be occupied by the constituent material, and thus expands the axial extent of the constituent material to make visual measurement of the constituent material more accurate.

    摘要翻译： 用于通常确定离心分离的材料混合物中的构成材料层的体积的技术。 使用离心管来保持材料混合物，并且在由待测量的构成材料占据的区域中的管中设置适当的成形体。 本体减少了可能被构成材料占据的管内的可用体积，从而扩大了构成材料的轴向范围，使组成材料的视觉测量更准确。

    Method and apparatus for determining the hematocrit of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
    8.
    发明授权
    Method and apparatus for determining the hematocrit of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells 有权
    利用红细胞内含有的血红蛋白的内在色素沉淀来测定血液样品的血细胞比容的方法和装置

    公开(公告)号：US08133738B2

    公开(公告)日：2012-03-13

    申请号：US13116837

    申请日：2011-05-26

    申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn Unfricht ,  Niten V. Lalpuria

    发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn Unfricht ,  Niten V. Lalpuria

    IPC分类号： G01N33/48 ,  G01N33/86

    CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

    摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.

    摘要翻译： 提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到适于静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和 高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。

    METHOD AND APPARATUS FOR DETERMINING AT LEAST ONE HEMOGLOBIN RELATED PARAMETER OF A WHOLE BLOOD SAMPLE
    9.
    发明申请
    METHOD AND APPARATUS FOR DETERMINING AT LEAST ONE HEMOGLOBIN RELATED PARAMETER OF A WHOLE BLOOD SAMPLE 有权
    用于确定至少一种HEMOGLOBIN相关参数的全血样品的方法和装置

    公开(公告)号：US20110256573A1

    公开(公告)日：2011-10-20

    申请号：US13051705

    申请日：2011-03-18

    申请人： Graham Davis ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten Lalpuria ,  Paul G. Mitsis

    发明人： Graham Davis ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten Lalpuria ,  Paul G. Mitsis

    IPC分类号： C12Q1/02 ,  C12M1/34

    CPC分类号： G01N33/726 ,  G01N15/1475 ,  G01N2015/0073

    摘要： A method and apparatus for determining at least one hemoglobin related parameter of a whole blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; b) imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; c) determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces; and d) determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.

    摘要翻译： 提供了一种用于确定全血样品的至少一种血红蛋白相关参数的方法和装置。 该方法包括以下步骤：a）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板的内表面和第二面板的内表面限定，并且所述室具有 在所述面板的内表面之间延伸的高度，其中所述腔被配置为在进入所述腔室之后的预定量的时间内将所述样品的氧合状态增加到基本上氧合状态; b）对与内表面接触的至少一个红细胞进行成像，并产生图像信号; c）确定接触两个内表面的成像红细胞的至少一部分的光密度; 以及d）使用确定的光密度和氧合血红蛋白的摩尔消光系数来确定与内表面接触的红细胞的至少一种血红蛋白相关参数。

    METHOD AND APPARATUS FOR DETERMINING THE HEMATOCRIT OF A BLOOD SAMPLE UTILIZING THE INTRINSIC PIGMENTATION OF HEMOGLOBIN CONTAINED WITHIN THE RED BLOOD CELLS
    10.
    发明申请
    METHOD AND APPARATUS FOR DETERMINING THE HEMATOCRIT OF A BLOOD SAMPLE UTILIZING THE INTRINSIC PIGMENTATION OF HEMOGLOBIN CONTAINED WITHIN THE RED BLOOD CELLS 有权
    用于确定使用红细胞中含有的HEMOGLOBIN的内在色素的血液样品的HEMATOCRIT的方法和装置

    公开(公告)号：US20110230740A1

    公开(公告)日：2011-09-22

    申请号：US13116837

    申请日：2011-05-26

    申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

    发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

    IPC分类号： A61B5/1455

    CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

    摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.

    摘要翻译： 提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到适于静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和 高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。