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公开(公告)号：US07741036B2
公开(公告)日：2010-06-22
申请号：US11331978
申请日：2006-01-13
CPC分类号： C12Q1/689 , C12Q1/686 , C12Q2600/156 , C12Q2565/627
摘要： Method for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) which is then matched against a database of base composition signatures, by which the bioagent is identified.
摘要翻译： 通过核酸扩增和分子量测定的组合来检测和识别未知生物标签的方法，所述引物与衍生自生物制剂的核酸的保守序列区域杂交的引物以及独特的括号可变序列区域 识别生物制剂。 结果是“基本组成签名”（BCS），然后与基础组合签名的数据库进行匹配，通过该数据库识别生物标签。
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公开(公告)号：US07095017B2
公开(公告)日：2006-08-22
申请号：US10939753
申请日：2004-09-13
IPC分类号： H01J49/00
CPC分类号： H01J49/04
摘要： The present disclosure is related to improved systems and methods for delivering samples for high-throughput mass spectrometric analysis to an atmospheric-pressure ionization source. In an exemplary embodiment, the system includes a solvent reservoir for storing a solvent solution, a first valve which is coupled to the solvent reservoir, first and second pumps for delivering solvent solution and which are coupled to the first valve and which the delivery flow rate of the first pump is greater than the delivery flow rate of the second pump, an injection system having a sample injector and an second valve which is coupled to the first valve and which is capable of being coupled to can be couple to an electrospray ionization source. In another embodiment, the system can also include an atmospheric-pressure ionization chamber, an atmospheric-pressure ionization sprayer and a nebulizer gas source and a voltage supply source. In yet another embodiment, the system may further include a puffer valve that is coupled to the nebulizer gas source and the atmospheric-pressure ionization sprayer and a gas puffer that is coupled to the puffer valve. A distal end of the gas puffer may be located within the atmospheric-pressure ionization chamber and aligned with the distal end of the atmospheric-pressure ionization sprayer and the puffer valve may control the delivery of the nebulizer gas to the atmospheric-pressure ionization sprayer and the gas puffer.
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公开(公告)号：US06787315B2
公开(公告)日：2004-09-07
申请号：US10209692
申请日：2002-07-31
IPC分类号： C12Q168
CPC分类号： G01N33/6842 , C12Q1/6811 , G01N33/566 , G01N33/6848 , G01N2500/02
摘要： Methods of identifying ligand binding sites in a target molecule such as a polynucleotide or polypeptide, as well as methods for determining whether a particular site in a target molecule is at or near the ligand binding site, are provided. Ligand binding affinities corresponding to both the target molecule and a modified version thereof are compared, preferably using mass spectral analysis. The modified molecules, or test molecules, incorporate a modification capable of modulating ligand binding affinity when located at or near the ligand binding sites. Binding site information is derived from the known position of the modification.
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公开(公告)号：US06730485B2
公开(公告)日：2004-05-04
申请号：US10210684
申请日：2002-07-31
IPC分类号： C12Q168
CPC分类号： G01N33/6842 , C12Q1/6811 , G01N33/566 , G01N33/6848 , G01N2500/02
摘要： Methods of identifying ligand binding sites in a target molecule such as a polynucleotide or polypeptide, as well as methods for determining whether a particular site in a target molecule is at or near the ligand binding site, are provided. Ligand binding affinities corresponding to both the target molecule and a modified version thereof are compared, preferably using mass spectral analysis. The modified molecules, or test molecules, incorporate a modification capable of modulating ligand binding affinity when located at or near the ligand binding sites. Binding site information is derived from the known position of the modification.
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公开(公告)号：US06730484B2
公开(公告)日：2004-05-04
申请号：US10210650
申请日：2002-07-31
IPC分类号： C12Q168
CPC分类号： G01N33/6842 , C12Q1/6811 , G01N33/566 , G01N33/6848 , G01N2500/02
摘要： Methods of identifying ligand binding sites in a target molecule such as a polynucleotide or polypeptide, as well as methods for determining whether a particular site in a target molecule is at or near the ligand binding site, are provided. Ligand binding affinities corresponding to both the target molecule and a modified version thereof are compared, preferably using mass spectral analysis. The modified molecules, or test molecules, incorporate a modification capable of modulating ligand binding affinity when located at or near the ligand binding sites. Binding site information is derived from the known position of the modification.
摘要翻译： 确定靶分子如多核苷酸或多肽中的配体结合位点的方法以及用于确定靶分子中特定位点是否在配体结合位点处或附近的方法。 比较对应于靶分子及其修饰形式的配体结合亲和力，优选使用质谱分析。 当位于配体结合位点处或附近时，修饰的分子或测试分子掺入能够调节配体结合亲和力的修饰。 绑定站点信息来自修改的已知位置。
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公开(公告)号：US06653067B1
公开(公告)日：2003-11-25
申请号：US09791147
申请日：2001-02-22
IPC分类号： C12Q170
CPC分类号： G01N33/6842 , C12Q1/6811 , G01N33/566 , G01N33/6848 , G01N2500/02
摘要： Methods of identifying ligand binding sites in a target molecule such as a polynucleotide or polypeptide, as well as methods for determining whether a particular site in a target molecule is at or near the ligand binding site, are provided. Ligand binding affinities corresponding to both the target molecule and a modified version thereof are compared, preferably using mass spectral analysis. The modified molecules, or test molecules, incorporate a modification capable of modulating ligand binding affinity when located at or near the ligand binding sites. Binding site information is derived from the known position of the modification.
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公开(公告)号：US09550990B2
公开(公告)日：2017-01-24
申请号：US11721326
申请日：2005-12-12
IPC分类号： C12N15/11 , C12N15/113
CPC分类号： C12N15/113 , C12N15/11 , C12N15/111 , C12N2310/11 , C12N2310/14 , C12N2310/315 , C12N2310/321 , C12N2310/3341 , C12N2310/341 , C12N2310/346 , C12N2320/11 , C12N2320/12 , C12N2330/10 , Y10T436/143333 , C12N2310/3525
摘要： Methods are provided for the identification of compounds that selectively modulate epigenetic changes in gene expression. Compounds, compositions, kits or assays devices, and methods are provided for modulating the expression, endogenous levels or the function of small non-coding RNAs cognate to or transcribed by heterochromatic regions subject to epigenetic regulation (i.e., promoters, enhancers, centromeres, telomeres, origins of DNA replication, imprinted loci, or loci marked by dosage-compensation), and for modulating the formation or function of heterochromatin in cells, tissues or animals.
摘要翻译： 提供了用于鉴定选择性调节基因表达的表观遗传变化的化合物的方法。 提供化合物，组合物，试剂盒或测定装置和方法用于调节受表观遗传调节的异染色体区域（即启动子，增强子，着丝粒，端粒）相关或转录的小非编码RNA的表达，内源水平或功能 ，DNA复制起点，印迹位点或由剂量补偿标记的基因座），并调节细胞，组织或动物中异染色质的形成或功能。
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48.发明授权Compositions comprising alternating 2′-modified nucleosides for use in gene modulation 有权包含交替的2'-修饰核苷的组合物用于基因调控公开(公告)号：US09150605B2
公开(公告)日：2015-10-06
申请号：US10860265
申请日：2004-06-03
申请人： Charles Allerson , Balkrishen Bhat , Anne B. Eldrup , Muthiah Manoharan , Richard H. Griffey , Brenda F. Baker , Eric E. Swayze
发明人： Charles Allerson , Balkrishen Bhat , Anne B. Eldrup , Muthiah Manoharan , Richard H. Griffey , Brenda F. Baker , Eric E. Swayze
IPC分类号： C07H21/02 , C07H21/00 , C07H21/04 , C12N15/11 , C12N15/113
CPC分类号： C12N15/113 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/322 , C12N2310/323 , C12N2320/30 , C12N2320/51
摘要： The present invention provides compositions comprising at least one oligomeric compound comprising an alternating motif and further include a region that is complementary to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.
摘要翻译： 本发明提供包含至少一种包含交替基序的低聚化合物并且还包括与核酸靶互补的区域的组合物。 该组合物可用于靶向选定的核酸分子并调节一个或多个基因的表达。 在优选的实施方案中，本发明的组合物与靶RNA的一部分杂交导致靶RNA的正常功能丧失。 本发明还提供了调节基因表达的方法。
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49.发明授权Oligomeric compounds and compositions for use in modulation of small non-coding RNAs 有权用于调节小非编码RNA的低聚化合物和组合物公开(公告)号：US08697663B2
公开(公告)日：2014-04-15
申请号：US12345725
申请日：2008-12-30
CPC分类号： C12N15/113 , C12N15/111 , C12N2310/11 , C12N2310/113 , C12N2310/14 , C12N2310/141 , C12N2310/31 , C12N2310/315 , C12N2310/317 , C12N2310/3181 , C12N2310/32 , C12N2310/321 , C12N2310/3231 , C12N2310/3341 , C12N2310/341 , C12N2310/346 , C12N2310/351 , C12N2310/3515 , C12N2320/11 , C12N2320/30 , C12N2320/50 , C12N2310/3525
摘要： Compounds, compositions and methods are provided for modulating the expression and function of small non-coding RNAs. The compositions comprise oligomeric compounds, targeted to small non-coding RNAs. Methods of using these compounds for modulation of small non-coding RNAs as well as downstream targets of these RNAs and for diagnosis and treatment of disease associated with small non-coding RNAs are also provided.
摘要翻译： 提供化合物，组合物和方法用于调节小非编码RNA的表达和功能。 组合物包含靶向小的非编码RNA的寡聚化合物。 还提供了使用这些化合物调节小非编码RNA以及这些RNA的下游靶标以及用于诊断和治疗与小非编码RNA相关的疾病的方法。
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公开(公告)号：US08394947B2
公开(公告)日：2013-03-12
申请号：US11569939
申请日：2004-03-03
申请人： Balkrishen Bhat , Eric E. Swayze , Thazha P. Prakash , Charles Allerson , Prasad Dande , Richard H. Griffey
发明人： Balkrishen Bhat , Eric E. Swayze , Thazha P. Prakash , Charles Allerson , Prasad Dande , Richard H. Griffey
CPC分类号： C12N15/113 , C12N15/111 , C12N15/1137 , C12N2310/14 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/3231 , C12N2320/50 , C12Y301/03048 , C12N2310/3525 , C12N2310/3521
摘要： The present invention provides oligomeric compounds having sufficient complementarity to hybridize to a nucleic acid target and methods for their use in modulating gene expression. In one embodiment the oligomeric compounds comprise double stranded constructs wherein one of the strands capable of hybridizing to a nucleic acid target, and has a plurality of modified ribofuranosyl nucleosides at defined locations. The presence of modifications at such defined positions greatly enhances the properties of the corresponding compositions.
摘要翻译： 本发明提供具有与核酸靶杂交的足够互补性的低聚化合物及其用于调节基因表达的方法。 在一个实施方案中，寡聚化合物包含双链构建体，其中能够与核酸靶杂交的一条链，并且在限定位置具有多个经修饰的呋喃核糖核苷。 在这样的定义的位置存在的修饰大大增强了相应组合物的性质。
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