利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

52 条记录 (耗时 0.026 秒)

    Ready-to-use whole blood collection vessel
    41.
    发明授权
    Ready-to-use whole blood collection vessel 有权
    即用型全血采集器

    公开(公告)号：US07863040B2

    公开(公告)日：2011-01-04

    申请号：US12327854

    申请日：2008-12-04

    申请人： Werner Naser ,  Thomas Duelffer ,  Rupert Herrmann ,  Uwe Kobold ,  Herbert von der Eltz

    发明人： Werner Naser ,  Thomas Duelffer ,  Rupert Herrmann ,  Uwe Kobold ,  Herbert von der Eltz

    IPC分类号： C12M1/33 ,  C12M3/08

    CPC分类号： G01N33/5002

    摘要： The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.

    摘要翻译： 本发明涉及一种用于收集和处理全血样本的取样管。 采样管含有用于全血差异溶血的试剂，其中所述用于差异溶血的试剂包括用于差异溶血的化学药剂和抗凝血剂，并且其中所述采样管是即用型和一次性采样管。 它还涉及在液相色谱法的全血样品的处理中使用所述采样管以及在基于液相色谱的分析中使用在这种采样管中处理的血液样品。

    Process for detecting nucleic acids by mass determination
    43.
    发明授权
    Process for detecting nucleic acids by mass determination 有权
    通过质量测定检测核酸的方法

    公开(公告)号：US06235476B1

    公开(公告)日：2001-05-22

    申请号：US09242536

    申请日：1999-03-17

    申请人： Frank Bergmann ,  Rupert Herrmann ,  Uwe Kobold

    发明人： Frank Bergmann ,  Rupert Herrmann ,  Uwe Kobold

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6872 ,  C12Q1/6827 ,  Y10T436/143333

    摘要： A method for detecting nucleic acids by binding a probe P to a partial sequence S contained in the nucleic acid to produce a binding product B1, degrading the nucleic acid to produce a binding product B2 containing a partial nucleic acid F of a defined length and detecting the binding product B2 or the partial nucleic acid F a part based on its mass is particularly suitable for the parallel detection of nucleic acid of different sequences.

    摘要翻译： 通过将探针P与核酸中所含的部分序列S结合以产生结合产物B1来检测核酸的方法，使核酸降解以产生含有限定长度的部分核酸F的结合产物B2并检测 结合产物B2或部分核酸F基于其质量的部分特别适用于不同序列的核酸的平行检测。

    Agent for the detection of substances with hydrolase activity
    44.
    发明授权
    Agent for the detection of substances with hydrolase activity 失效
    用于检测具有水解酶活性的物质的试剂

    公开(公告)号：US5260428A

    公开(公告)日：1993-11-09

    申请号：US988975

    申请日：1993-01-29

    申请人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

    发明人： Rupert Herrmann ,  Hans-Joachim Guder ,  Werner Guthlein ,  Manfred Kuhr ,  Johann Berger ,  Harvey Buck

    IPC分类号： C12Q1/34 ,  G01N33/58 ,  C07H15/24 ,  C07C43/00 ,  C07C63/36 ,  C07C303/00 ,  C07H15/00

    CPC分类号： C12Q1/34 ,  G01N33/581 ,  C12Q2334/30 ,  G01N2333/924

    摘要： A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidising agent, and the evaluating the resultant colour intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical,R.sup.3, R.sup.4, R.sup.5, R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, andX is a glycosyl, phosphate or acyl residue. Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.

    摘要翻译： 一种用于通过将样品与水解酶底物和氧化剂混合来检测样品中水解酶活性的物质并评估所得颜色强度的方法，其中作为水解酶底物，使用至少一种式 其中R 1是氢或烷氧基，R 2是氢或卤素，氨基或烷氧基或芳烷氧基，可以相同或不同的R 3，R 4，R 5，R 6是氢，卤素， 羧基，氨基甲酰基，磺基，氨基烷基，烷氧基，芳烷氧基，烷基羰基或烷氧基羰基，X是糖基，磷酸酯或酰基残基。 任选地，R 2和R 3，R 3和R 4，R 4和R 5以及R 5和R 6可以连接形成环系。 R 1和R 2中的至少一个必须是氢，R 1，R 2和R 3中的至少一个不能是氢。 X是β-半乳糖基的化合物是新的。

    USE OF A BIS-MALEIC ANHYDRIDE CROSS-LINKING AGENT FOR FIXATION OF A CELL OR TISSUE SAMPLE
    46.
    发明申请
    USE OF A BIS-MALEIC ANHYDRIDE CROSS-LINKING AGENT FOR FIXATION OF A CELL OR TISSUE SAMPLE 失效
    用于固定细胞或组织样品的双相无定形交联剂交联剂

    公开(公告)号：US20120149019A1

    公开(公告)日：2012-06-14

    申请号：US13400862

    申请日：2012-02-21

    申请人： Hans-Peter Josel ,  Michael Gerg ,  Rupert Herrmann ,  Rosa Isabel Saez Diaz

    发明人： Hans-Peter Josel ,  Michael Gerg ,  Rupert Herrmann ,  Rosa Isabel Saez Diaz

    IPC分类号： C12Q1/68 ,  C07D407/12 ,  C07D407/06 ,  G01N1/36 ,  G01N33/567

    CPC分类号： G01N1/30

    摘要： The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like.

    摘要翻译： 本公开涉及新颖的双马来酸酐，以及令人惊奇的发现，即双马来酸酐交联剂可用于保存/固定细胞或组织样品。 各种双马来酸酐交联剂扫描用于需要固定细胞或组织样品的方法。 这些试剂和方法在需要除去固定剂以便于与其它试剂分析的方法中特别有用。 本发明的试剂和方法使得可以使用诸如免疫组织化学，荧光原位杂交，RT-PCR等的分析方法更容易地可靠地测定各种蛋白质，核酸等。