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    SEMI-SYNTHETIC TEREPHTHALIC ACID VIA MICROORGANISMS THAT PRODUCE MUCONIC ACID
    42.
    发明申请
    SEMI-SYNTHETIC TEREPHTHALIC ACID VIA MICROORGANISMS THAT PRODUCE MUCONIC ACID 审中-公开
    通过生产肌酸的微生物的半合成的正十二酸

    公开(公告)号：US20140302573A1

    公开(公告)日：2014-10-09

    申请号：US14308292

    申请日：2014-06-18

    Applicant: Genomatica, Inc.

    Inventor： Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun

    IPC: C12P7/44

    CPC classification number: C12P7/44 ,  C12N9/88 ,  C12Y402/01002

    Abstract: The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-CoA hydrolase, a beta-ketoadipyl-CoA transferase, a beta-ketoadipyl-CoA ligase, a 2-fumarylacetate reductase, a 2-fumarylacetate dehydrogenase, a trans-3-hydroxy-4-hexendioate dehydratase, a 2-fumarylacetate aminotransferase, a 2-fumarylacetate aminating oxidoreductase, a trans-3-amino-4-hexenoate deaminase, a beta-ketoadipate enol-lactone hydrolase, a muconolactone isomerase, a muconate cycloisomerase, a beta-ketoadipyl-CoA dehydrogenase, a 3-hydroxyadipyl-CoA dehydratase, a 2,3-dehydroadipyl-CoA transferase, a 2,3-dehydroadipyl-CoA hydrolase, a 2,3-dehydroadipyl-CoA ligase, a muconate reductase, a 2-maleylacetate reductase, a 2-maleylacetate dehydrogenase, a cis-3-hydroxy-4-hexendioate dehydratase, a 2-maleylacetate aminoatransferase, a 2-maleylacetate aminating oxidoreductase, a cis-3-amino-4-hexendioate deaminase, and a muconate cis/trans isomerase. Other muconate pathway enzymes also are provided. Additionally provided are methods of producing muconate.

    Abstract translation: 本发明提供了一种非天然存在的微生物，其具有一种具有至少一种外源核酸的粘蛋白途径的微生物生物，所述外源核酸编码以足够量表达的粘蛋白途径酶以产生粘蛋白。 所述粘蛋白途径包括选自β-酮硫解酶，β-酮己二酰辅酶A水解酶，β-酮己二酰辅酶A转移酶，β-酮己二酰辅酶A连接酶，2-富马酰乙酸酯还原酶，2- 富马酰乙酸脱氢酶，反式-3-羟基-4-己二酸脱水酶，2-富马酸乙酯氨基转移酶，2-富马酸乙酯胺化氧化还原酶，反式-3-氨基-4-己烯酸脱氨酶，β-酮己二酸烯醇内酯水解酶， 粘蛋白内酯异构酶，粘蛋白环异构酶，β-酮己二酰辅酶A脱氢酶，3-羟基己二酰辅酶A脱水酶，2,3-脱氢阿迪普基-CoA转移酶，2,3-脱氢阿迪普基-CoA水解酶，2,3-脱氢阿迪普酯 - CoA连接酶，粘蛋白还原酶，2-马来酰乙酸酯还原酶，2-马来酰乙酸酯脱氢酶，顺式-3-羟基-4-己二酸脱水酶，2-马来酰乙酸氨基转移酶，2-马来酰乙酸胺胺化氧化还原酶，顺式-3-氨基 -4-十六酸脱氨酶和粘蛋白顺式/反式异构酶。 还提供了其它粘蛋白途径酶。 另外提供了生产粘蛋白的方法。

    METHODS FOR INCREASING PRODUCT YIELDS
    43.
    发明申请
    METHODS FOR INCREASING PRODUCT YIELDS 审中-公开
    增加产品线的方法

    公开(公告)号：US20140011249A1

    公开(公告)日：2014-01-09

    申请号：US13889056

    申请日：2013-05-07

    Applicant: Genomatica, Inc.

    Inventor： Anthony P. BURGARD ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

    IPC: C12P7/42 ,  C12P7/04 ,  C12P7/18

    CPC classification number: C12P7/42 ,  C12N9/0008 ,  C12N9/0095 ,  C12P7/04 ,  C12P7/16 ,  C12P7/18 ,  C12P7/52 ,  Y02E50/10

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

    Abstract translation: 非天然存在的微生物体包括具有还原性TCA或Wood-Ljungdahl途径的微生物生物体，其中至少一种编码这些途径酶的外源核酸以足够的量表达以增强通过乙酰辅酶A的碳通量。 通过乙酰辅酶A增强碳通量的方法包括在条件和足够的时间段内培养这些非天然存在的微生物，以产生具有乙酰辅酶A作为结构单元的产物。 另一种非天然存在的微生物生物体包括至少一种编码以足够量表达的酶的外源核酸，以增强在一氧化碳或氢气存在下还原当量的可用性，从而通过碳水化合物 - 碳原料。 在一氧化碳或氢气存在下增强还原当量的可用性的方法包括在足够的时间内培养该生物体以产生产物。

    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID
    44.
    发明申请
    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID 审中-公开
    丙二酸，十六烷二胺和6-氨基酸的生物合成的微生物和方法

    公开(公告)号：US20130303723A1

    公开(公告)日：2013-11-14

    申请号：US13730612

    申请日：2012-12-28

    Applicant: Genomatica, Inc.

    Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

    IPC: C12P17/12 ,  C12P13/00

    CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

    Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

    Abstract translation: 本发明提供了具有6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径的非天然存在的微生物生物。 微生物生物体含有至少一种编码各自的6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径中的酶的外源核酸。 本发明另外提供了制备6-氨基己酸，己内酰胺，己二胺或乙酰丙酸的方法。 该方法可以包括培养6-氨基己酸，己内酰胺或六亚甲基二胺生产微生物，其中微生物生物体表达至少一种编码6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径酶的外源核酸，其量足以产生 各自的产物在条件下和足够的时间内产生6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸。

    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL
    48.
    发明申请
    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL 有权

    公开(公告)号：US20220348932A1

    公开(公告)日：2022-11-03

    申请号：US17516424

    申请日：2021-11-01

    Applicant: Genomatica, Inc.

    Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Stefan Andrae

    IPC: C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12N9/04 ,  C12N9/10 ,  C12P7/04

    Abstract: The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

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