公开(公告)号：US20110036974A1

公开(公告)日：2011-02-17

申请号：US12857161

申请日：2010-08-16

Applicant: Jochen Franzen ,  Claus Köster

Inventor： Jochen Franzen ,  Claus Köster

IPC: H01J49/04

CPC classification number: H01J49/062 ,  H01J49/0445

Abstract: Charged droplets are guided along a defined path from a droplet source to a droplet sink. A focusing pseudopotential distribution generated by audio frequencies on electrodes of a guiding device guide the charged droplets from the droplet source to the droplet sink with low loss. The droplets can be driven along the droplet guide by a gas flow, an axial electric field or a combination of both. For example, charged droplets from a spray capillary of an electrospray ion source at atmospheric pressure may be introduced into the inlet capillary leading to the vacuum system of ion analyzers, a procedure similar to that used up to now in nanoelectrospraying, but with substantially higher flow rates. In the guiding device, the droplets can be manipulated in different ways, for example evaporated down to a desired size. The introduction of small droplets into gas-aspirating capillaries is of interest because it is possible to keep the droplets on axis by Bernoulli focusing and to guide them in large quantities and with low loss through the capillary. The ability to guide the droplets makes it also possible to install a segmented inlet capillary with intermediate pumping, which allows pumping capacity to be saved. Advantageously, the sensitivity of ion analyzers such as mass spectrometers or ion mobility spectrometers by at least one order of magnitude.

Abstract translation: 带电液滴沿着从液滴源到液滴槽的限定路径被引导。 由引导装置的电极上的音频产生的聚焦伪势分布将带电液滴从液滴源引导到具有低损耗的液滴槽。 液滴可以通过气流，轴向电场或两者的组合沿液滴引导件驱动。 例如，在大气压下来自电喷雾离子源的喷雾毛细管的带电液滴可以引入入口毛细管中，导入离子分析仪的真空系统，该方法与迄今为止在纳电电喷雾中使用的方法类似，但具有显着更高的流动 价格。 在引导装置中，液滴可以以不同的方式进行操作，例如蒸发到所需尺寸。 将小液滴引入吸气毛细管是令人感兴趣的，因为可以通过伯努利聚焦将液滴保持在轴上，并且通过毛细管大量引导并以低损耗引导。 引导液滴的能力使得还可以安装具有中间泵送的分段入口毛细管，这允许节省泵送能力。 有利地，诸如质谱仪或离子迁移谱仪的离子分析仪的灵敏度至少有一个数量级。

公开(公告)号：US20100224775A1

公开(公告)日：2010-09-09

申请号：US12716813

申请日：2010-03-03

Applicant: Andreas Haase ,  Jens Höhndorf ,  Jochen Franzen

Inventor： Andreas Haase ,  Jens Höhndorf ,  Jochen Franzen

IPC: H01J49/26 ,  H01J49/02

CPC classification number: H01J49/164 ,  H01J49/0045

Abstract: Mass spectrometry with lasers generates ions from analyte molecules by matrix assisted laser desorption for a variety of different mass spectrometric analysis procedures. The mass spectrometers with laser systems supply laser light pulses having at least two different pulse durations, and mass spectrometric measuring techniques use the laser light pulses of different durations. The duration of the laser light pulses allows the characteristics of the ionization of the analyte molecules, particularly the occurrence of the ISD (in-source decay) and PSD (post-source decay) types of fragmentation, whose fragment ion spectra supply different kinds of information, to be adapted to the analytic procedure.

Abstract translation: 使用激光的质谱法通过基质辅助激光解吸从分析物分子产生离子，用于各种不同的质谱分析程序。 具有激光系统的质谱仪提供具有至少两个不同脉冲持续时间的激光脉冲，并且质谱测量技术使用不同持续时间的激光脉冲。 激光脉冲的持续时间允许分析物分子的电离特性，特别是ISD（源内衰变）和PSD（后源衰变）类型的断裂的出现，其碎片离子谱提供不同种类的 信息，以适应分析程序。

公开(公告)号：US07595486B2

公开(公告)日：2009-09-29

申请号：US11691166

申请日：2007-03-26

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: H01J49/04

CPC classification number: H01J49/065 ,  H01J49/063

Abstract: In a multipole rod ion guide system operated with RF voltages to collect or transmit ions, the inhomogeneity of the electric RF fields is increased in front of the ion guide rods by forming the rod surfaces from a plurality of spaced electrodes. The inhomogeneous fields produced by the plurality of electrodes increases the mass range over which the ions are guided effectively while still maintaining a pseudopotential minimum which is as well defined as possible close to the axis. Particularly favorable ion guides of this type make it possible to apply an axial DC field to the guide system for the active transport of the ions.

Abstract translation: 在用RF电压操作以收集或传输离子的多极杆离子导向系统中，通过从多个间隔开的电极形成杆表面，在离子导杆的前方增加了电RF场的不均匀性。 由多个电极产生的不均匀场增加离子被有效引导的质量范围，同时仍保持尽可能靠近轴线限定的赝势最小值。 这种特别有利的离子导向器使得可以将轴向DC场施加到导向系统以有源输送离子。

公开(公告)号：US07399640B2

公开(公告)日：2008-07-15

申请号：US10828153

申请日：2004-04-20

Applicant: Martin Schürenberg ,  Jochen Franzen

Inventor： Martin Schürenberg ,  Jochen Franzen

IPC: G01N24/00 ,  B01J19/08 ,  H01J49/40

CPC classification number: H01J49/0418 ,  B01L3/5085 ,  G01N1/40 ,  Y10T436/11 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention relates to sample support plates with hydrophilic anchors in a strongly hydrophobic environment for mass spectroscopic analysis of biosubstances with ionization by matrix-assisted laser desorption and ionization (MALDI), procedures for manufacturing these sample supports, and connected procedures for loading these sample supports with biomolecular samples. The invention provides areas with affinity adsorbents adjacent to the hydrophilic anchors for purifying biosubstances and, if wanted, for performing an affinity selection of biosubstances, whereby the finally prepared matrix sample crystals with the biosubstances for the MALDI analysis are well localized on the hydrophilic anchors.

Abstract translation: 本发明涉及在强疏水环境中具有亲水性锚定物的样品支撑板，用于通过基质辅助激光解吸和电离（MALDI）进行离子化生物物质的质谱分析，制备这些样品载体的方法以及用于加载这些样品载体的连接方法 与生物分子样品。 本发明提供了亲水性吸附剂的邻近亲水性锚定物的区域，用于纯化生物条件，并且如果需要，进行生物物质的亲和选择，由此最终制备的具有用于MALDI分析的生物基质的基质样品晶体很好地定位在亲水性锚定体上。

公开(公告)号：US20070272848A1

公开(公告)日：2007-11-29

申请号：US11695796

申请日：2007-04-03

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: B01D59/44

CPC classification number: H01J49/04 ,  H01J49/004 ,  H01J49/401 ,  H01J49/4225

Abstract: In a time-of-flight mass spectrometer with orthogonal ion injection performed by a pulser to which the ions are fed by an RF ion guide, compensation is provided for mass discrimination that occurs when the ions are injected into the pulser. This is accomplished by designing at least a part of the ion guide as an ion storage device, by emptying the filled ion storage device mass-selectively in ion groups, group-by-group, and by serially feeding the ion groups to the pulser with correct timing, using the mass selectivity of the pulser filling process to compensate for the mass discrimination.

Abstract translation: 在由离子由RF离子导向器馈送的脉冲发生器进行的具有正交离子注入的飞行时间质谱仪中，提供了当离子注入脉冲发生器时发生的质量鉴别的补偿。 这是通过将离子导向器的至少一部分设计为离子存储装置来实现的，通过以离子基团的形式大量选择性地排空填充的离子存储装置，并且通过将离子基团串联馈送到脉冲发生器， 正确的时间，使用脉冲发生器填充过程的质量选择性来补偿质量鉴别。

公开(公告)号：US07196326B2

公开(公告)日：2007-03-27

申请号：US11149952

申请日：2005-06-10

Applicant: Jochen Franzen ,  Evgenij Nikolaev

Inventor： Jochen Franzen ,  Evgenij Nikolaev

IPC: B01D59/44 ,  H01J49/42 ,  H01J49/04

CPC classification number: H01J49/062 ,  H01J49/0077 ,  H01J49/0095

Abstract: The invention relates to a reaction cell for reactions between different types of ion species and a related mass spectrometer to analyze the ion products. The invention consists in an RF-operated straight ion guide with a side inlet, particularly suitable for reactions between positive and negative ion species, one ion species being fed in through the side inlet. Particularly favorable is an ion guide made up of a set of coaxial apertured diaphragms with a slight axial potential gradient. The reactions can be used for a fragmentation of multiply charged protein or peptide ions by electron transfer, or for the removal of excess charges of multiply charged biopolymer ions, for example.

Abstract translation: 本发明涉及用于不同类型离子种类之间的反应的反应池和相关的质谱仪来分析离子产物。 本发明包括具有侧入口的RF操作的直离子导向器，特别适用于正离子和负离子之间的反应，一个离子种类通过侧入口进料。 特别有利的是由具有轻微轴向电位梯度的一组同轴孔径隔膜组成的离子导向器。 该反应可以用于例如通过电子转移分离多电荷蛋白质或肽离子，或用于除去多电荷生物聚合物离子的过量电荷。

公开(公告)号：US07164125B2

公开(公告)日：2007-01-16

申请号：US11090316

申请日：2005-03-25

Applicant: Gerhard Weiss ,  Jochen Franzen ,  Carsten Stoermer

Inventor： Gerhard Weiss ,  Jochen Franzen ,  Carsten Stoermer

IPC: H01J49/42

CPC classification number: H01J49/04 ,  H01J49/443

Abstract: The invention relates to two-dimensional quadrupole systems along whose axis an axial DC field is superimposed. The invention involves coating the hyperbolic or cylindrical surfaces of quadrupole systems with thin insulating layers and metal films thereupon and generating axial potential gradients or saddle ramps using appropriate electrical supply of DC potentials and superimposed RF voltages to the metal films. Systems of this type can be used in a plurality of ways, ranging from mass filters with high transmission to fragmentation cells with extremely low ion losses.

Abstract translation: 本发明涉及二维四极杆系统，其轴上的轴向DC场被叠加。 本发明涉及使用薄的绝缘层和金属膜涂覆四极杆系统的双曲面或圆柱形表面，并使用合适的DC电势和叠加的RF电压产生轴向电位梯度或鞍形斜坡。 这种类型的系统可以以多种方式使用，从具有高透射率的质量过滤器到具有极低离子损失的碎裂单元。

公开(公告)号：US20060226357A1

公开(公告)日：2006-10-12

申请号：US11243510

申请日：2005-10-04

Applicant: Jochen Franzen ,  Evgenij Nikolaev

Inventor： Jochen Franzen ,  Evgenij Nikolaev

IPC: G01N23/00

CPC classification number: H01J49/38

Abstract: The invention relates to measuring methods and corresponding measuring cells for ion cyclotron resonance mass spectrometers (FTMS). The invention provides measuring methods with measuring cells, the ends of which each incorporate a large number of trapping electrodes, DC voltages of opposite polarities being applied across adjacent electrodes. For orbiting ions this builds up a repelling pseudopotential, which holds the ions in the measuring cell by reflection. This facilitates measurement of the image currents without the disturbing influence of RF voltages

Abstract translation: 本发明涉及用于离子回旋共振质谱仪（FTMS）的测量方法和相应的测量单元。 本发明提供了测量单元的测量方法，其测量单元的端部各自包含大量的捕获电极，相邻极性的直流电压被施加在相邻的电极之间。 对于轨道离子，这建立了排斥赝势，通过反射将离子保持在测量池中。 这有助于图像电流的测量，而不受RF电压的干扰影响

公开(公告)号：US06995366B2

公开(公告)日：2006-02-07

申请号：US10861232

申请日：2004-06-04

Applicant: Jochen Franzen

Inventor： Jochen Franzen

IPC: H01J39/34

CPC classification number: H01J49/4225 ,  H01J49/0054

Abstract: The invention relates to a method and device for the fragmentation of macromolecules in linear quadrupole RF ion traps according to Wolfgang Paul. The invention consists in fragmenting the ions by the capture of low energy electrons (ECD), injected into the linear RF ion trap. One way of doing this is to inject low energy electrons through the gap between the pole rods. Another possibility is to inject the electrons through an opening in one of the pole rods carrying a RF voltage, the electron source being kept at the highest positive potential which is achieved on the center axis of the ion trap during the RF period. Both methods can be improved by pulse-shaped RF voltages, offering longer periods for electron capture. The electron beam can be guided by a magnetic field.

Abstract translation: 本发明涉及根据沃尔夫冈·保罗（Wolfgang Paul）在线性四极杆射频离子阱中分解大分子的方法和装置。 本发明通过捕获注入到线性RF离子阱中的低能电子（ECD）来分离离子。 这样做的一个方法是通过极杆之间的间隙注入低能电子。 另一种可能性是通过承载RF电压的极杆之一中的开口注入电子，电子源保持在RF周期内在离子阱的中心轴上实现的最高正电位。 两种方法都可以通过脉冲形RF电压来提高，为电子捕获提供更长的时间。 电子束可以由磁场引导。

公开(公告)号：US20050274887A1

公开(公告)日：2005-12-15

申请号：US11090316

申请日：2005-03-25

Applicant: Gerhard Weiss ,  Jochen Franzen ,  Carsten Stoemer

Inventor： Gerhard Weiss ,  Jochen Franzen ,  Carsten Stoemer

IPC: B01D59/44 ,  G21K1/093 ,  H01J37/141 ,  H01J49/04 ,  H01J49/06 ,  H01J49/42 ,  H01J49/44

CPC classification number: H01J49/04 ,  H01J49/443

Abstract: The invention relates to two-dimensional quadrupole systems along whose axis an axial DC field is superimposed. The invention involves coating the hyperbolic or cylindrical surfaces of quadrupole systems with thin insulating layers and metal films thereupon and generating axial potential gradients or saddle ramps using appropriate electrical supply of DC potentials and superimposed RF voltages to the metal films. Systems of this type can be used in a plurality of ways, ranging from mass filters with high transmission to fragmentation cells with extremely low ion losses.

Abstract translation: 本发明涉及二维四极杆系统，其轴上的轴向DC场被叠加。 本发明涉及使用薄的绝缘层和金属膜涂覆四极杆系统的双曲面或圆柱形表面，并使用合适的DC电势和叠加的RF电压产生轴向电位梯度或鞍形斜坡。 这种类型的系统可以以多种方式使用，从具有高透射率的质量过滤器到具有极低离子损失的碎裂单元。