公开(公告)号：US20070038419A1

公开(公告)日：2007-02-15

申请号：US11501232

申请日：2006-08-09

申请人： Yoshihiro Usuda ,  Shintaro Iwatani ,  Kazuhiko Matsui

发明人： Yoshihiro Usuda ,  Shintaro Iwatani ,  Kazuhiko Matsui

IPC分类号： G06F17/10

CPC分类号： G16B5/00

摘要： A method for simulating a substance production process using cells based on a set of differential equations that represent intracellular metabolites and gene expression comprising including a specific growth rate of cells, expressed as a differential equation, in the set of differential equations; assigning values for parameters in the set of differential equations, wherein at least one of said parameters is represented as a growth rate factor; incorporating in the set of differential equations a formation rate for formation of a cell component, wherein said formation rate is represented as a growth rate factor; incorporating in the set of differential equations an inflow rate of a metabolite taken from outside of the cells and/or an outflow rate of a metabolite excreted out of the cells, wherein said inflow/outflow rates are represented as a growth rate factor; solving the set of differential equations; and generating data representative of the substance-production process.

摘要翻译： 一种基于代表细胞内代谢物和基因表达的微分方程组，使用细胞模拟物质生产过程的方法，其包括在微分方程组中包含表示为微分方程的细胞的比生长速率; 为所述微分方程组中的参数分配值，其中所述参数中的至少一个被表示为增长率因子; 在微分方程组中并入形成细胞成分的形成速率，其中所述形成速率表示为生长速率因子; 在微分方程组中引入从细胞外部获取的代谢物的流入速率和/或从细胞排出的代谢物的流出速率，其中所述流入/流出速率表示为生长速率因子; 求解微分方程组; 并产生代表物质生产过程的数据。

公开(公告)号：US06210951B1

公开(公告)日：2001-04-03

申请号：US09550338

申请日：2000-04-14

申请人： Hisashi Kawasaki ,  Yoshihiro Usuda ,  Yasuhiro Mihara ,  Osamu Kurahashi

发明人： Hisashi Kawasaki ,  Yoshihiro Usuda ,  Yasuhiro Mihara ,  Osamu Kurahashi

IPC分类号： C12N120

CPC分类号： C12N9/93

摘要： A protein which has a GMP synthetase activity, a DNA which codes for the protein and a transformant which is introduced with the DNA in a form that allows expression of the protein encoded by the DNA.

摘要翻译： 具有GMP合成酶活性的蛋白质，编码该蛋白质的DNA和以允许由DNA编码的蛋白质表达的形式引入DNA的转化体。

公开(公告)号：US08551741B2

公开(公告)日：2013-10-08

申请号：US12479010

申请日：2009-06-05

申请人： Yoshihiro Usuda ,  Seizaburo Shiraga ,  Kazuhiko Matsui ,  Shigeo Suzuki

发明人： Yoshihiro Usuda ,  Seizaburo Shiraga ,  Kazuhiko Matsui ,  Shigeo Suzuki

IPC分类号： C12P13/04

CPC分类号： C12N1/20 ,  C12N1/38 ,  C12P13/04

摘要： An L-amino acid is produced by culturing a bacterium of the Enterobacteriaceae family which has an L-amino acid-producing ability in a medium containing fatty acids as the carbon source, particularly fatty acids which have been subjected to emulsification or homogenization, to thereby produce and accumulate the L-amino acid in a culture medium; and collecting the L-amino acid from the culture medium.

摘要翻译： 通过在含有脂肪酸作为碳源的培养基中，特别是经过乳化或均化的脂肪酸培养具有L-氨基酸生产能力的肠杆菌科的细菌来生产L-氨基酸，由此 在培养基中产生并积累L-氨基酸; 并从培养基中收集L-氨基酸。

公开(公告)号：US08510053B2

公开(公告)日：2013-08-13

申请号：US10933280

申请日：2004-09-03

申请人： Yosuke Nishio ,  Eiichiro Kimura ,  Yoshihiro Usuda ,  Kazuho Ikeo ,  Yoji Nakamura ,  Takashi Gojobori ,  Yutaka Kawarabayashi ,  Yumi Hino ,  Eiichi Hori ,  Jun Yamazaki

发明人： Yosuke Nishio ,  Eiichiro Kimura ,  Yoshihiro Usuda ,  Kazuho Ikeo ,  Yoji Nakamura ,  Takashi Gojobori ,  Yutaka Kawarabayashi ,  Yumi Hino ,  Eiichi Hori ,  Jun Yamazaki

IPC分类号： G01N33/50

CPC分类号： C12N9/88 ,  C12N9/00 ,  C12N9/0016 ,  C12N9/1205 ,  C12N15/1034 ,  C12N15/1089

摘要： The present invention relates to a method for modifying a property of a protein. The present invention also relates to a method for producing a protein which has a modified property, and a method for producing a microorganism which has a modified property. The present invention is useful in the field of microbial industrial production and the like.

摘要翻译： 本发明涉及一种改变蛋白质性质的方法。 本发明还涉及具有改性特性的蛋白质的制造方法，以及具有改性的微生物的制造方法。 本发明在微生物工业生产等领域中是有用的。

公开(公告)号：US08367371B2

公开(公告)日：2013-02-05

申请号：US12420934

申请日：2009-04-09

申请人： Yoshinori Tajima ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui

发明人： Yoshinori Tajima ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P1/00

CPC分类号： C12N9/88 ,  C12P13/08 ,  C12P13/227

摘要： A bacterium which belongs to the Enterobacteriaceae family and has an ability to produce an L-amino acid such as L-lysine, L-threonine and L-tryptophan and is modified to enhance glutamic acid decarboxylase activity is cultured in a medium to produce and accumulate the L-amino acid in the medium or cells of the bacterium. Then, the L-amino acid is collected from the medium or the cells.

摘要翻译： 属于肠杆菌科的具有生产L-赖氨酸，L-苏氨酸和L-色氨酸的L-氨基酸的能力并经过修饰以增强谷氨酸脱羧酶活性的细菌在培养基中培养以产生和积累 细菌的培养基或细胞中的L-氨基酸。 然后，从培养基或细胞收集L-氨基酸。

公开(公告)号：US08354254B2

公开(公告)日：2013-01-15

申请号：US12842355

申请日：2010-07-23

申请人： Shigeo Suzuki ,  Yoshihiro Usuda ,  Shuhei Hashiro

发明人： Shigeo Suzuki ,  Yoshihiro Usuda ,  Shuhei Hashiro

IPC分类号： C12P13/04

CPC分类号： C12P13/04 ,  C12P13/08 ,  C12P13/14

摘要： An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.

摘要翻译： 通过在含有促进L-氨基酸的生成和积累的微藻的加工物的培养基中培养具有L-氨基酸生产能力的细菌来生产L-氨基酸。 通过破坏微藻的培养和/或提取微藻的培养物，或分馏微藻的培养物或破坏的培养物来生产该过程产物。 经加工的产品含有由微藻生产的有机物质，破碎的微藻培养物的水解产物和/或微藻培养物的提取物或分馏产物的混合物。 经加工的产品还可以含有淀粉或脂肪和油的水解产物的糖化产物。 培养细菌以在培养物中产生和积累L-氨基酸，并从培养物中收集L-氨基酸。

公开(公告)号：US20120315678A1

公开(公告)日：2012-12-13

申请号：US13474879

申请日：2012-05-18

申请人： Shuhei Hashiro ,  Yoshihiro Usuda

发明人： Shuhei Hashiro ,  Yoshihiro Usuda

IPC分类号： C12N1/12 ,  C12P13/04 ,  C13K1/00

CPC分类号： C12R1/89 ,  C12N1/12 ,  C12P13/04 ,  C12P19/02 ,  C12P19/04

摘要： Glucose is produced by hydrolyzing starch contained in a microalga which belongs to the genus Desmodesmus and accumulates 30% or more of starch in algae bodies based on dry weight of the algae bodies when it is cultured under suitable conditions.

摘要翻译： 葡萄糖通过水解属于脱虫纲的微藻中含有的淀粉而产生，并且在适当条件下培养时，基于藻体的干重，在藻体中累积30％或更多的淀粉。

公开(公告)号：US20120219995A1

公开(公告)日：2012-08-30

申请号：US13404148

申请日：2012-02-24

申请人： Hidetaka Doi ,  Yoshihiro Usuda

发明人： Hidetaka Doi ,  Yoshihiro Usuda

IPC分类号： C12P13/04 ,  C12P13/22 ,  C12P13/12 ,  C12P13/08

CPC分类号： C12P13/08 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/78 ,  C12N9/80 ,  C12P13/12 ,  C12P13/227 ,  C12Y203/01109 ,  C12Y206/01081 ,  C12Y305/01096 ,  C12Y305/03023

摘要： A method for producing an L-amino acid which includes the steps of culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid producing ability in a medium to produce and accumulate an L-amino acid in the medium, and collecting the L-amino acid from the medium, wherein the bacterium has been modified so that an activity or activities of one or two or more enzymes of the arginine succinyltransferase pathway, such as arginine succinyltransferase, succinylarginine dihydrolase, succinylornithine aminotransferase, succinylglutamate-semialdehyde dehydrogenase, and succinylglutamate desuccinylase, is/are decreased.

摘要翻译： 一种L-氨基酸的制造方法，其特征在于，在培养基中培养属于肠杆菌科并具有L-氨基酸生产能力的细菌的步骤，在培养基中生成并积累L-氨基酸，并收集 来自培养基的L-氨基酸，其中所述细菌已被修饰，使得精氨酸琥珀酰转移酶途径的一种或两种或多种酶的活性或活性，例如精氨酸琥珀酰转移酶，琥珀酰精氨酸二水解酶，琥珀酰鸟氨酸氨基转移酶，琥珀酰谷氨酸 - 半醛脱氢酶和 琥珀酰谷氨酸脱琥珀酰基酶降低。

公开(公告)号：US07809511B2

公开(公告)日：2010-10-05

申请号：US11337580

申请日：2006-01-24

申请人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Takuji Ueda ,  Yuichiro Tsuji

发明人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Takuji Ueda ,  Yuichiro Tsuji

IPC分类号： G06F19/00

CPC分类号： C12N9/0006 ,  C12P13/08

摘要： A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.

摘要翻译： 影响使用细胞的物质生产的代谢通量是通过以下方法确定的：1）根据基质通过所需生产物质的生物化学反应的公式产生化学计量矩阵，2）从所有代谢通量中选择相同数量的独立代谢通量， 作为自由通量的化学计量矩阵的自由度，3）产生足够数量的自由通量的随机组合用于统计分析，并且基于化学计量矩阵计算每个创建的组合的代谢通量分布，4）获得包括 通过多变量统计分析从计算的代谢通量分布中显示与物质生产相关的最小数量的自由通量，以及5）基于所获得的回归方程中的系数确定影响物质生产的至少一种代谢通量。

公开(公告)号：US20100093044A1

公开(公告)日：2010-04-15

申请号：US12635323

申请日：2009-12-10

申请人： Masaru Terashita ,  Yoshihiro Usuda ,  Kazuhiko Matsui

发明人： Masaru Terashita ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/22 ,  C12P13/08 ,  C12P13/06

CPC分类号： C12N9/0008 ,  C12P13/06 ,  C12P13/08 ,  C12P13/222 ,  C12P13/227

摘要： A microorganism is provided which has an ability to produce an L-amino acid such as L-lysine, L-tryptophan, L-phenylalanine, L-valine, L-leucine, L-isoleucine and L-serine, and has been modified to increase the activity of pyruvate synthase or pyruvate:NADP+ oxidoreductase. This microorganism is cultured in a medium containing ethanol or an aliphatic acid as the carbon source to produce and accumulate the L-amino acid in the medium or cells, and the L-amino acid is collected from the medium or the cells.

摘要翻译： 提供了具有产生L-赖氨酸，L-色氨酸，L-苯丙氨酸，L-缬氨酸，L-亮氨酸，L-异亮氨酸，L-丝氨酸等L-氨基酸的能力的微生物， 增加丙酮酸合酶或丙酮酸：NADP +氧化还原酶的活性。 将该微生物在含有乙醇或脂肪酸作为碳源的培养基中培养，以在培养基或细胞中产生并积累L-氨基酸，从培养基或细胞收集L-氨基酸。