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    Direct RT-PCR on oligonucleotide-immobilized PCR microplates
    43.
    发明授权
    Direct RT-PCR on oligonucleotide-immobilized PCR microplates 有权
    对寡核苷酸固定的PCR微孔板进行直接RT-PCR

    公开(公告)号：US06844158B1

    公开(公告)日：2005-01-18

    申请号：US10048800

    申请日：1998-12-22

    申请人： Masato Mitsuhashi

    发明人： Masato Mitsuhashi

    IPC分类号： C12N1/06 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6837 ,  C12N1/06 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2565/501 ,  C12Q2521/107

    摘要： The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which oligonucleotides are securely immobilized and which can be subjected to thermal cycles of PCR. RT-PCR is preferably conducted in solid-phase. Capturing of mRNA and RT-PCR can be conducted in the same plates. The cDNA synthesized from the mRNA captured on the microplates can be used more than once. Further, in combination with the microplates, a filter plate is used for the preparation of cell lysates wherein target cells are placed on the filter plate, and a lysis buffer is passed through the cell layer on the filter to transfer cell lysate directly to the microplate via well-to-well communication.

    摘要翻译： 通过使用由例如聚丙烯制成的寡核苷酸固定的微板，其中寡核苷酸被可靠地固定并且可以进行PCR的热循环来简化逆转录聚合酶链反应（RT-PCR）的整个过程。 RT-PCR优选在固相中进行。 捕获mRNA和RT-PCR可以在相同的平板上进行。 从微孔板上捕获的mRNA合成的cDNA可以多次使用。 此外，与微板相结合，过滤板用于制备细胞裂解物，其中将靶细胞置于滤板上，使裂解缓冲液通过过滤器上的细胞层，将细胞裂解液直接转移至微板 通过良好的沟通。

    Method for quantifying total mRNA with poly(A)-complementary oligonucleotide-immobilized microtiter plate
    44.
    发明授权
    Method for quantifying total mRNA with poly(A)-complementary oligonucleotide-immobilized microtiter plate 失效
    用聚（A） - 互补寡核苷酸固定化微量滴定板定量总mRNA的方法

    公开(公告)号：US5976797A

    公开(公告)日：1999-11-02

    申请号：US772150

    申请日：1996-12-20

    申请人： Masato Mitsuhashi

    发明人： Masato Mitsuhashi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6846 ,  C12Q1/6851

    摘要： A method for quantifying total mRNA in a biological sample containing RNA such as crude cell lysates containing cytosolic mRNA, which method comprises the steps of: (a) incubating the sample with an oligo-(dT)- or poly-U-immobilized microtiter plate; (b) washing non-hybridized components from the microtiter plate; (c) labeling the hybridized mRNA with a photometric nucleic-acid dye; (d) measuring the amount of label captured on the microtiter plate; (e) heat-denaturing the labeled mRNA; (f) washing the denatured mRNA from the microtiter plate; and (g) measuring the amount of label remaining on the microtiter plate; and (h) correlating the amount of the measured label (captured label minus remaining label) with the quantity of total mRNA present in the sample, thereby easily measuring the total mRNA without the influence of rRNA or tRNA and without radioactive dyes, which method can be adapted to chemosensitivity tests.

    摘要翻译： 一种用于定量含有RNA的生物样品中含有胞质mRNA的粗细胞裂解物的总mRNA的方法，该方法包括以下步骤：（a）将样品与寡固体（dT）或聚-U-固定的微量滴定板 ; （b）从微量滴定板洗涤非杂交组分; （c）用光度核酸染料标记杂交的mRNA; （d）测量在微量滴定板上捕获的标签的量; （e）对标记的mRNA进行热变性; （f）从微量滴定板洗涤变性的mRNA; 和（g）测量微量滴定板上剩余的标签的量; 和（h）将测量的标记物（捕获的标记物减去剩余标签）的量与样品中存在的总mRNA的量相关联，由此容易地测量总mRNA，而不受rRNA或tRNA的影响，而不使用放射性染料，哪种方法可以 适应化学敏感性测试。

    Fungal detection system based on rRNA probes
    45.
    发明授权
    Fungal detection system based on rRNA probes 失效
    基于rRNA探针的真菌检测系统

    公开(公告)号：US5580971A

    公开(公告)日：1996-12-03

    申请号：US379081

    申请日：1995-01-26

    申请人： Masato Mitsuhashi

    发明人： Masato Mitsuhashi

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6895

    摘要： The invention includes methods for the detection of a particular genus or species of fungus in a biological sample. Some of these methods make use of a solid support-polynucleotide structure. This structure includes a solid support having immobilized thereto a polynucleotide probe that is complementary to a sequence of ribosomal RNA (rRNA) specific to the particular species of fungus. An rRNA sequence from the particular species of fungus is hybridized to the first probe, and a second polynucleotide probe is also hybridized to the rRNA.

    摘要翻译： 本发明包括用于检测生物样品中特定属或种真菌的方法。 这些方法中的一些使用固体支持物 - 多核苷酸结构。 该结构包括其固定有固体支持物的多核苷酸探针，其与特定的真菌种特异性的核糖体RNA（rRNA）序列互补。 来自特定真菌种类的rRNA序列与第一个探针杂交，第二个多核苷酸探针也与rRNA杂交。

    Liquid ejecting apparatus
    48.
    发明授权
    Liquid ejecting apparatus 有权
    液体喷射装置

    公开(公告)号：US08100492B2

    公开(公告)日：2012-01-24

    申请号：US12411571

    申请日：2009-03-26

    申请人： Masato Mitsuhashi ,  Makoto Kawamoto ,  Koji Higuchi

    发明人： Masato Mitsuhashi ,  Makoto Kawamoto ,  Koji Higuchi

    IPC分类号： B41J29/38

    CPC分类号： B41J3/4075 ,  B41J2/16526 ,  B41J2/16585 ,  B41J11/0085 ,  B41J2002/16591 ,  B41J2002/1742

    摘要： A liquid ejecting apparatus includes a liquid ejecting head disposed on a transport path of a target to eject liquid from nozzles at a nozzle forming surface, a target transport unit transporting the target on the basis of a driving force of a first driving source such that the target passes through a position opposite the nozzle forming surface on the transport path, a liquid receptor transport unit transporting a liquid receptor for receiving the liquid ejected from the nozzles as a waste liquid on the basis of a driving force of a second driving source such that the liquid receptor passes through the position opposite the nozzle forming surface on the transport path, and a control unit controlling a driving state of the second driving source in order to change a transport velocity of the liquid receptor by the liquid receptor transport unit while the liquid receptor is transported.

    摘要翻译： 一种液体喷射装置，包括：设置在目标物的输送路径上的液体喷射头，用于从喷嘴形成面喷嘴排出液体，目标传送单元，基于第一驱动源的驱动力传送目标物， 目标通过与传送路径上的喷嘴形成表面相对的位置，液体接收器传送单元，其基于第二驱动源的驱动力，传送液体接收器，用于接收从喷嘴喷射的液体作为废液，使得 液体受体通过与传送路径上的喷嘴形成表面相对的位置，以及控制单元，其控制第二驱动源的驱动状态，以便改变液体受体传送单元的液体受体的输送速度，同时液体 受体被运送。

    PUMA, A PRO-APOPTOTIC GENE, AS A NOVEL MOLECULAR BIOMARKER FOR TNFalpha-INDUCED HUMAN ISLET DAMAGE
    49.
    发明申请
    PUMA, A PRO-APOPTOTIC GENE, AS A NOVEL MOLECULAR BIOMARKER FOR TNFalpha-INDUCED HUMAN ISLET DAMAGE 有权
    PUMA，一种PRO-APOPTOTIC基因，作为TNFα诱导的人类免疫缺陷病毒的新型分子生物标志物

    公开(公告)号：US20110318751A1

    公开(公告)日：2011-12-29

    申请号：US13163326

    申请日：2011-06-17

    申请人： Yoko MULLEN ,  Masato Mitsuhashi ,  Keiko Omori

    发明人： Yoko MULLEN ,  Masato Mitsuhashi ,  Keiko Omori

    IPC分类号： C12Q1/68 ,  G01N33/566 ,  C12N5/071

    CPC分类号： C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/507 ,  G01N2333/4748 ,  G01N2333/525

    摘要： p53-upregulated modulator of apoptosis (PUMA) is a biomarker associated with islet cell health. If PUMA is low, islet cells are typically healthy. If PUMA is high, islet cells are typically unhealthy or dying. PUMA may be measured by either measuring its nucleic or amino acid. PUMA mRNA may be induced by TNF-α stimulation in a time- and dose-dependent manner and β cell apoptosis is induced through a mitochondrial pathway. TNF-α significantly inhibited glucose-induced preproinsulin precursor mRNA synthesis. Such β cell stress signaling in human islets indicates overall state of islet health and, ultimately, the risk of onset and/or degree of severity of both type 1 and type 2 diabetes mellitus.

    摘要翻译： p53上调凋亡调节剂（PUMA）是与胰岛细胞健康相关的生物标志物。 如果PUMA低，胰岛细胞通常是健康的。 如果PUMA高，胰岛细胞通常不健康或死亡。 PUMA可以通过测量其核酸或氨基酸来测量。 PUMA mRNA可能以时间和剂量依赖性方式诱导TNF-α刺激， 通过线粒体途径诱导细胞凋亡。 TNF-α显着抑制葡萄糖诱导的前胰岛素前体mRNA合成。 这样的 人胰岛中的细胞应激信号表示胰岛健康的总体状态，最终表明1型和2型糖尿病的发病风险和/或严重程度。

    Method for predicting immune response to neoplastic disease based on mRNA expression profile in neoplastic cells and stimulated leukocytes
    50.
    发明授权
    Method for predicting immune response to neoplastic disease based on mRNA expression profile in neoplastic cells and stimulated leukocytes 有权
    基于肿瘤细胞和刺激白细胞中mRNA表达谱预测肿瘤疾病免疫应答的方法

    公开(公告)号：US07741023B2

    公开(公告)日：2010-06-22

    申请号：US11917151

    申请日：2006-06-08

    申请人： Masato Mitsuhashi

    发明人： Masato Mitsuhashi

    IPC分类号： C12Q1/68 ,  G01N33/48 ,  G01N33/574

    CPC分类号： G01N33/505 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158 ,  G01N33/574

    摘要： Tumor necrosis factor (TNF) is capable of inducing apoptosis by interacting with specific TNF receptors on the surface of cancer cells. Because multiple members of TNF ligand and receptor are present within each superfamily, over 300 different ligand-receptor combinations exist. Activated blood leukocytes produce TNF as part of the immune response to cancer, as well as producing chemokines to attract other leukocytes to the site. A method is disclosed of detecting significant induction of a variety of TNF superfamily subtype and chemokine mRNAs in blood leukocytes when whole blood is exposed to heat-aggregated IgG or anti-T cell receptor antibodies as a model of immune system interactions. Substantial individual-to-individual variation is observed in TNF subtypes and chemokines induced. Since peripheral blood leukocytes are the supply of anti-cancer immune cells, the quantitation of ex vivo inducibility of appropriate TNF ligands and chemokines in blood will be useful in individualized cancer immunotherapy. If the tumor mass is small, such as with early invisible metastatic lesions, appropriate TNF assaults may be sufficient to prevent relapse.

    摘要翻译： 肿瘤坏死因子（TNF）能够通过与癌细胞表面的特异性TNF受体相互作用诱导细胞凋亡。 由于TNF配体和受体的多个成员存在于每个超家族中，所以存在超过300种不同的配体 - 受体组合。 活化的血液白细胞产生TNF作为对癌症的免疫应答的一部分，以及产生趋化因子以吸引其他白细胞到该部位。 公开了当全血暴露于作为免疫系统相互作用的模型的热聚集的IgG或抗T细胞受体抗体时，检测血白细胞中各种TNF超家族亚型和趋化因子mRNA的显着诱导的方法。 在TNF亚型和趋化因子诱导中观察到显着的个体与个体差异。 由于外周血白细胞是抗癌免疫细胞的供应，所以在个体化的癌症免疫治疗中定量适当的TNF配体和趋化因子在血液中的离体诱导性将是有用的。 如果肿瘤质量小，如早期隐形转移性病变，适当的TNF攻击可能足以防止复发。