公开(公告)号：US07799557B2

公开(公告)日：2010-09-21

申请号：US11080705

申请日：2005-03-15

申请人： Kwang-wook Oh ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park ,  Yoon-kyoung Cho

发明人： Kwang-wook Oh ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park ,  Yoon-kyoung Cho

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： G01N21/6486 ,  B01L3/50851 ,  B01L7/52 ,  B01L2300/0627 ,  B01L2300/18 ,  G01N21/0332

摘要： Provided are a DNA PCR module and a multiple PCR system using the same. More particularly, provided are a DNA PCR module with a combined PCR thermal cycler and PCR product detector, and a multiple PCR system using the same.

摘要翻译： 提供了DNA PCR模块和使用其的多重PCR系统。 更具体地，提供了具有组合的PCR热循环仪和PCR产物检测器的DNA PCR模块，以及使用其的多重PCR系统。

公开(公告)号：US07698072B2

公开(公告)日：2010-04-13

申请号：US11217694

申请日：2005-09-01

申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

IPC分类号： G06F19/00

CPC分类号： C12Q1/6851

摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。

公开(公告)号：US07452669B2

公开(公告)日：2008-11-18

申请号：US10931522

申请日：2004-09-01

申请人： Sang-hyo Kim ,  Jung-im Han ,  Chin-sung Park ,  Jin-tae Kim

发明人： Sang-hyo Kim ,  Jung-im Han ,  Chin-sung Park ,  Jin-tae Kim

IPC分类号： C12Q1/68

CPC分类号： B01L3/502707 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2300/16 ,  G01N27/021

摘要： A micro PCR device comprising an amplification chamber is provided. The amplification chamber has an inner surface coated with a polycationic polymer or a polyanionic polymer and includes electrodes.

摘要翻译： 提供了包括放大室的微型PCR装置。 扩增室具有涂覆有聚阳离子聚合物或聚阴离子聚合物的内表面并且包括电极。

公开(公告)号：US07364857B2

公开(公告)日：2008-04-29

申请号：US11257833

申请日：2005-10-25

申请人： Yoon-kyoung Cho ,  Sook-young Kim ,  Jin-tae Kim ,  Kyu-sang Lee

发明人： Yoon-kyoung Cho ,  Sook-young Kim ,  Jin-tae Kim ,  Kyu-sang Lee

IPC分类号： C12Q1/68

CPC分类号： C12N15/1006 ,  C12Q1/6806 ,  C12Q1/70 ,  Y10S977/924 ,  C12Q2563/155 ,  C12Q2523/308

摘要： Provided is a method of purifying a target substance using silver nanoparticles. The method includes: mixing a sample containing molecules having a thiol group with the silver nanoparticles to obtain a complex of the molecules having the thiol group with the silver nanoparticles; and isolating and removing the complex from the mixture. By using the purification method, PCR amplifiable DNAs can be rapidly purified, and thus, the method can be very efficiently applied to lab-on-chip (LOC).

摘要翻译： 提供了使用银纳米粒子来净化目标物质的方法。 该方法包括：将含有硫醇基的分子的样品与银纳米颗粒混合，得到具有硫醇基的分子与银纳米颗粒的络合物; 并从混合物中分离并除去络合物。 通过使用纯化方法，可以快速纯化PCR扩增的DNA，因此该方法可以非常有效地应用于片上实验（LOC）。

公开(公告)号：US20060258012A1

公开(公告)日：2006-11-16

申请号：US11335414

申请日：2006-01-19

申请人： Ji-yeon Yang ,  Yoon-kyoung Cho ,  Jin-tae Kim ,  Sook-young Kim ,  Young-sun Lee

发明人： Ji-yeon Yang ,  Yoon-kyoung Cho ,  Jin-tae Kim ,  Sook-young Kim ,  Young-sun Lee

IPC分类号： G01N33/50

CPC分类号： C12N1/066 ,  C12M47/06 ,  C12M47/20 ,  Y10T436/25

摘要： Provided is a cell lysis method including: preparing a cell sample to be lysed; heating the cell sample; and cooling the cell sample by causing an endothermic reaction near the cell sample. According to the method, cell lysis can be simply and conveniently performed without regard to location and without additional devices since a separate energy source is not required and the apparatus is portable. In particular, when cell lysis is performed in a biochip using a small amount of sample, a greater cell lysis effect can be obtained. In addition, cell lysis efficiency is significantly improved, compared to when only heating is performed.

公开(公告)号：US20060078931A1

公开(公告)日：2006-04-13

申请号：US11245348

申请日：2005-10-06

申请人： Kwang-wook Oh ,  Yu-jin Seo ,  Gyeong-sik Ok ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park

发明人： Kwang-wook Oh ,  Yu-jin Seo ,  Gyeong-sik Ok ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/502715 ,  B01L9/527 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2400/065

摘要： Provided is a microchip unit, including a microchip on which a plurality of micro-channels are formed, a housing disposed below the microchip to fix the microchip; and at least two injecting and sealing elements having through-holes corresponding to inlets of the microchip. The injecting and sealing elements are vertically fixed on the top of the housing and slide in a horizontal direction from a first location to a second location and vice versa. The through-holes are aligned with inlets of the microchip so that a reaction solution can be injected through the through-holes when the injecting and sealing elements are placed at the first location. The inlets of the microchip are sealed by elastic members formed on bottom surfaces of the injecting and sealing elements when the injecting and sealing elements are placed at the second location.

摘要翻译： 提供一种微芯片单元，其包括形成有多个微通道的微芯片，设置在微芯片下面以固定微芯片的壳体; 以及至少两个具有对应于微芯片的入口的通孔的注入和密封元件。 注射和密封元件垂直地固定在壳体的顶部上，并且在从第一位置到第二位置的水平方向上滑动，反之亦然。 通孔与微芯片的入口对准，使得当注射和密封元件放置在第一位置时，反应溶液可以通过通孔注入。 当注射和密封元件放置在第二位置时，微芯片的入口由形成在注射和密封元件的底表面上的弹性构件密封。

公开(公告)号：US20050140978A1

公开(公告)日：2005-06-30

申请号：US10933084

申请日：2004-09-02

申请人： Su-hyeon Kim ,  Jin-tae Kim

发明人： Su-hyeon Kim ,  Jin-tae Kim

IPC分类号： C12M1/00 ,  C12M1/34 ,  G01N21/03 ,  G01N21/64 ,  G01N21/78 ,  G01N21/88

CPC分类号： G01N21/645 ,  G01N2021/6482

摘要： An ultra small fluorescence detector capable of detecting in real time reaction undergoing in a micro chamber having a predetermined volume and disposed on a microfluid chip is provided. The fluorescence detector for detecting in real time PCR amplification undergoing in the microfluid chip having a micro chamber with a predetermined volume includes a light source generating an excitation beam, a first optical system capable of irradiating the excitation beam having a predetermined spot size to the micro chamber, a first detector, and a second optical system reflecting a fluorescent beam derived from the excitation beam having the predetermined spot size in the micro chamber to the first detector. Accordingly, the fluorescence detector is designed such that light emitted by a light source is focused between a first mirror and an objective lens. Therefore, the spot size of an excitation beam transmitted by the objective lens is largely formed so that the excitation beam can be irradiated on the whole micro chamber of the microfluid chip, thereby detecting a fluorescent beam on a broader area.

摘要翻译： 提供了能够实时检测具有预定体积的微室中并设置在微流体芯片上的超小荧光检测器。 用于实时检测在具有预定体积的微室的微流体芯片中进行PCR扩增的荧光检测器包括产生激发光束的光源，能够将具有预定光斑尺寸的激发光束照射到微细的第一光学系统 第一检测器和第二光学系统，其将来自具有在微室中的预定光斑尺寸的激发光束的荧光束反射到第一检测器。 因此，荧光检测器被设计成使得由光源发射的光聚焦在第一反射镜和物镜之间。 因此，由物镜透射的激发光束的光斑尺寸大大地形成为使得激发光束可以照射在微流体芯片的整个微室上，从而在更广泛的区域上检测荧光束。