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47 条记录 (耗时 0.04 秒)

    ALLELIC DISCRIMINATION ANALYSIS USING AN EFFICIENCY RELATED VALUE (EFR)
    41.
    发明申请
    ALLELIC DISCRIMINATION ANALYSIS USING AN EFFICIENCY RELATED VALUE (EFR) 审中-公开
    使用效率相关价值（EFR）的ALLELIC DISCRIMINATION ANALYSIS

    公开(公告)号：US20120156682A1

    公开(公告)日：2012-06-21

    申请号：US13315133

    申请日：2011-12-08

    申请人： Eric B. Shain

    发明人： Eric B. Shain

    IPC分类号： C12Q1/68 ,  G06F19/00 ,  C12M1/34

    CPC分类号： G16B30/00 ,  C12Q1/6858 ,  G16B40/00 ,  C12Q2537/165

    摘要： In various embodiments this invention provides novel methods for discriminating two or more different target nucleic acids. In certain embodiments the methods comprise providing data amplification reactions comprising reagents to amplify two or more different target nucleic acids where the data comprise signals comprising an amplitude measurement representing the degree of amplification of each target nucleic acid in the amplification reaction and the time point in the amplification reaction at which the amplitude is measured; determining an efficiency related transform of the data, determining an efficiency related value for each target nucleic acid that is the maximum magnitude of the efficiency related transform; and outputting the efficiency related values in the amplification reaction for each target nucleic acid, where the relative amplitudes of the efficiency related values for each target nucleic acid is an indicator of the presence of each of said nucleic acids in said sample.

    摘要翻译： 在各种实施方案中，本发明提供用于区分两种或更多种不同靶核酸的新方法。 在某些实施方案中，所述方法包括提供包含用于扩增两种或更多种不同靶核酸的试剂的数据扩增反应，其中所述数据包括包括表示扩增反应中每个靶核酸的扩增程度的振幅测量的信号，以及 测量振幅的扩增反应; 确定所述数据的效率相关变换，确定作为所述效率相关变换的最大幅度的每个靶核酸的效率相关值; 并输出针对每个靶核酸的扩增反应中的效率相关值，其中每个靶核酸的效率相关值的相对幅度是所述样品中每种所述核酸的存在的指标。

    Diagnostic assay for a sample of biological fluid
    44.
    发明授权
    Diagnostic assay for a sample of biological fluid 失效
    生物流体样本的诊断测定

    公开(公告)号：US06312888B1

    公开(公告)日：2001-11-06

    申请号：US09688636

    申请日：2000-10-16

    申请人： Sie Ting Wong ,  Robert G. Hiltibran ,  Tung-Ming Huang ,  Brenda B. Calfin ,  Mark R. Pope ,  Thomas G. Schapira ,  Eric B. Shain ,  Douglas F. Young

    发明人： Sie Ting Wong ,  Robert G. Hiltibran ,  Tung-Ming Huang ,  Brenda B. Calfin ,  Mark R. Pope ,  Thomas G. Schapira ,  Eric B. Shain ,  Douglas F. Young

    IPC分类号： C12Q100

    CPC分类号： G01N33/558 ,  G01N33/525

    摘要： An article and a method for monitoring the concentration of an analyte, e. g., glucose, in blood. In one aspect, the invention involves an article comprising a multiple-layer element. In one embodiment, the article comprises: a multiple-layer element comprising: (a) a base layer (b) a cover layer, the cover layer having a first opening for venting the multiple-layer element and a second opening for receiving a biological sample; and (c) a core layer having a first major surface and a second major surface, the core layer disposed between the base layer and the cover layer, the core layer comprising a sample introduction chamber and a optical reading chamber, the first major surface of the core layer in face-to-face contact with the base layer, the second major surface of the core layer in face-to-face contact with the cover layer. The multiple-layer element of this invention allows the use of samples of blood or other biological fluids, such as interstitial fluid, to provide extremely sensitive assay results. The device has been shown to provide accurate and reproducible results with samples having volumes ranging from about 5 &mgr;L to about 20 &mgr;L. In another aspect, the invention involves a method for using the aforementioned article.

    摘要翻译： 用于监测分析物浓度的文章和方法， 例如血糖中的葡萄糖。 一方面，本发明涉及包含多层元件的制品。 在一个实施例中，所述制品包括：多层元件，包括：（a）基层（b）覆盖层，所述覆盖层具有用于排出所述多层元件的第一开口和用于接收生物 样品; 和（c）具有第一主表面和第二主表面的芯层，所述芯层设置在所述基底层和所述覆盖层之间，所述芯层包括样品引入室和光学读取室，所述芯层的第一主表面 芯层与基层面对面接触，芯层的第二主表面与覆盖层面对面接触。本发明的多层元件允许使用血液样品 或其他生物流体，例如间质液，以提供极敏感的测定结果。 该装置已被证明可以提供具有约5至20微米的体积的样品的准确和可重复的结果。 另一方面，本发明涉及使用上述制品的方法。

    Device and method for amplifying and detecting target nucleic acids
    47.
    发明授权
    Device and method for amplifying and detecting target nucleic acids 失效
    用于扩增和检测靶核酸的装置和方法

    公开(公告)号：US5645801A

    公开(公告)日：1997-07-08

    申请号：US516728

    申请日：1995-08-18

    申请人： Stanley R. Bouma ,  Ronald A. Coules ,  Julian Gordon ,  Eric B. Shain ,  Natalie A. Solomon ,  Peter Zaun

    发明人： Stanley R. Bouma ,  Ronald A. Coules ,  Julian Gordon ,  Eric B. Shain ,  Natalie A. Solomon ,  Peter Zaun

    IPC分类号： B01L7/00 ,  C12Q1/68 ,  C12M1/40 ,  C12P19/34

    CPC分类号： B01L7/52 ,  Y10S435/81

    摘要： Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then sealably mated with a detection chamber to form a sealed reaction/detection unit that is virtually irreversibly closed. One or more heating elements of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Kits include the reaction/detection units and reagents for amplification.

    摘要翻译： 提供了用于扩增和检测核酸的方法，装置，装置和试剂盒。 该装置是与反应/检测单元结合操作的热循环装置。 将样品装载到装置的反应室中，然后与检测室密封地配合，以形成几乎不可逆地封闭的密封的反应/检测单元。 热循环装置的一个或多个加热元件对反应/检测装置施加所需的温度以放大样品中的靶核酸。 然后将反应混合物转移到检测室，并将扩增的靶核酸固定在检测室中的载体上。 与该装置相关的检测系统检测并分析固定的扩增的核酸靶。 试剂盒包括用于扩增的反应/检测单元和试剂。