利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

47 条记录 (耗时 0.031 秒)

    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine
    41.
    发明授权
    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 失效
    棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法

    公开(公告)号：US06908754B2

    公开(公告)日：2005-06-21

    申请号：US10284334

    申请日：2002-10-31

    申请人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    发明人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    IPC分类号： C12N1/21 ,  C12N9/00 ,  C12P13/10 ,  C12N9/10 ,  C07K17/00 ,  C12N9/12

    CPC分类号： C12N9/93 ,  C12P13/10

    摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

    摘要翻译： 编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽 序列表所示的SEQ ID NO：2中的50〜393的酸值，（b）至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代， 一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质，其具有氨基酸序列SEQ ID NO：3的氨基甲酰磷酸合成酶的大亚基，（c ）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，（d）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，包括取代，缺失，插入，添加， 或反转一个或几个氨基酸，并可构成 具有氨基酸 - 磷酸合成酶活性的蛋白质，其具有SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。

    Dna encoding sucrose pts enzyme II
    42.
    发明授权
    Dna encoding sucrose pts enzyme II 失效
    Dna编码蔗糖酶Ⅱ

    公开(公告)号：US06893852B1

    公开(公告)日：2005-05-17

    申请号：US10019284

    申请日：2000-06-30

    申请人： Masako Izui ,  Masakazu Sugimoto ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

    发明人： Masako Izui ,  Masakazu Sugimoto ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

    IPC分类号： C12N9/12 ,  C12N15/54 ,  C07H21/04

    CPC分类号： C12N9/1205 ,  C12Y207/01069

    摘要： A gene encoding a protein constituting sucrose PTS of coryneform bacterium is provided by amplifying a region existing downstream from sucrase gene in coryneform bacterium by the cassette-ligation mediated PCR to obtain DNA encoding sucrose PTS enzyme II, which is a protein defined in the following (A) or (B): (A) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing; (B) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing including substitution, deletion, insertion, addition or inversion of one or several amino acids, and an activity for binding to sucrose.

    摘要翻译： 通过盒式连接介导的PCR扩增棒状杆菌型细菌的蔗糖酶基因下游的区域，得到编码构成棒状细菌的蔗糖PTS的蛋白质的基因，得到编码下述定义的蛋白质的蔗糖PTS酶II的DNA（ A）或（B）：（A）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质; （B）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或转化，以及与蔗糖结合的活性。

    Temperature sensitive plasmid for coryneform bacteria
    43.
    发明授权
    Temperature sensitive plasmid for coryneform bacteria 有权
    棒状细菌的温度敏感质粒

    公开(公告)号：US06303383B1

    公开(公告)日：2001-10-16

    申请号：US09521668

    申请日：2000-03-08

    申请人： Jun Nakamura ,  Sohei Kanno ,  Eiichiro Kimura ,  Kazuhiko Matsui ,  Tsuyoshi Nakamatsu

    发明人： Jun Nakamura ,  Sohei Kanno ,  Eiichiro Kimura ,  Kazuhiko Matsui ,  Tsuyoshi Nakamatsu

    IPC分类号： C12N1574

    CPC分类号： C12N15/77

    摘要： A coryneform bacterium in which a DNA fragment is incorporated into its chromosome is prepared by (a) obtaining a recombinant plasmid through ligation of a DNA fragment having a sequence homologous to a gene present on a chromosome of a coryneform bacterium to a plasmid that has a wild-type replication control region segment of a particular nucleotide sequence including a mutation and is autonomously replicable in a coryneform bacterium cell at a culture temperature lower than 31° C. but not autonomously replicable in the cell at a temperature of 31° C. or higher, (b) introducing the recombinant plasmid into the coryneform bacterium cell, (c) culturing the bacterium at a temperature of 31° C. or higher, (d) causing homologous recombination between the DNA fragment and the gene present on the chromosome of the coryneform bacterium and having a sequence homologous to the DNA fragment, and (e) selecting a coryneform bacterium in which the DNA fragment is incorporated into its chromosome. According to the present invention, there is provided a method for efficiently modifying genetic traits of a host in a short period of time by obtaining a temperature sensitive plasmid from a plasmid not exhibiting homology with already reported temperature sensitive plasmids or not exhibiting incompatibility therewith.

    摘要翻译： 通过（a）通过将具有与存在于棒状杆菌型细菌的染色体上的基因同源的序列的DNA片段连接到具有下列基因的质粒来获得重组质粒来制备将DNA片段并入其染色体中的棒状细菌： 包含突变的特定核苷酸序列的野生型复制控制区段，并且在棒状细菌细胞中，在低于31℃的培养温度下是可自主复制的，但在31℃的温度下不能在细胞中自主复制，或 （b）将重组质粒引入棒状细菌细胞中，（c）在31℃或更高的温度下培养细菌，（d）使DNA片段与存在于染色体上的基因之间的同源重组 所述棒状细菌具有与所述DNA片段同源的序列，和（e）选择其中所述DNA片段并入其染色体中的棒状细菌 e。 根据本发明，提供了一种通过从未报道的温度敏感性质粒不显示同源性的质粒或不表现出不相容性的质粒获得温度敏感性质粒来在短时间内高效地修饰宿主遗传性状的方法。

    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine
    44.
    发明授权
    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
    棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法

    公开(公告)号：US06255086B1

    公开(公告)日：2001-07-03

    申请号：US09629616

    申请日：2000-07-31

    申请人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    发明人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    IPC分类号： C12P1310

    CPC分类号： C12P13/10 ,  C12N9/93

    摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

    摘要翻译： 编码以下（a）或（b）中定义的多肽的DNA片段，以及以下（c）或（d）:( a）定义的多肽，其至少具有氨基酸的氨基酸序列 序列表所示的SEQ ID NO：2中的酸值为50〜393，（b）至少具有序列表所示的SEQ ID No：2的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代， 一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰基 - 磷酸合成酶活性的蛋白质，其具有氨基酸 - 磷酸合成酶的大亚基，其氨基酸序列至少包含氨基酸数55至 SEQ ID NO：3的1113，（c）具有至少包含序列表所示的SEQ ID NO：3的氨基酸编号55至1113的氨基酸序列的多肽，（d）具有氨基酸的多肽 序列至少包含氨基酸序号55至1113 o f序列号3所示，包括一个或多个氨基酸的取代，缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的氨基甲酰磷酸合成酶的小亚基的蛋白质， SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列。

    Methods of producing L-lysine and L-glutamic acid by fermentation
    46.
    发明授权
    Methods of producing L-lysine and L-glutamic acid by fermentation 失效
    通过发酵生产L-赖氨酸和L-谷氨酸的方法

    公开(公告)号：US5846790A

    公开(公告)日：1998-12-08

    申请号：US776597

    申请日：1997-02-18

    申请人： Eiichiro Kimura ,  Yoko Asakura ,  Akinori Uehara ,  Sumio Inoue ,  Yoshio Kawahara ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    发明人： Eiichiro Kimura ,  Yoko Asakura ,  Akinori Uehara ,  Sumio Inoue ,  Yoshio Kawahara ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    IPC分类号： C12N1/21 ,  C12N15/09 ,  C12P13/08 ,  C12P13/14

    CPC分类号： C12P13/14 ,  C12P13/08 ,  Y10S435/84 ,  Y10S435/843

    摘要： A mutant strain having an ability to produce L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to produce and accumulate L-glutamic acid in the medium. A mutant strain having an ability to produce L-lysine and L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent and giving L-lysine productivity to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to simultaneously produce and accumulate L-lysine and L-glutamic acid in the medium.

    摘要翻译： PCT No.PCT / JP95 / 01586 Sec。 371日期1997年2月18日 102（e）1997年2月18日PCT PCT 1995年8月9日PCT公布。 出版物WO96 / 06180 日期：1996年2月29日在具有生物素活性抑制剂的情况下，在含有过量生物素的培养基中，不存在生成L-谷氨酸的能力的突变株是通过赋予生物素作用抑制 代谢产L-谷氨酸棒状细菌。 将该菌株在液体培养基中培养以在培养基中产生并积累L-谷氨酸。 通过赋予生物素活性抑制剂的温度敏感度，获得在含有过量生物素的培养基中不存在任何生物素活性抑制剂时能够产生L-赖氨酸和L-谷氨酸的突变株 并向L-谷氨酸棒状杆菌产生细菌提供L-赖氨酸生产力。 该菌株在液体培养基中培养，以在培养基中同时产生和积累L-赖氨酸和L-谷氨酸。