Methods of producing L-lysine and L-glutamic acid by fermentation
    3.
    发明授权
    Methods of producing L-lysine and L-glutamic acid by fermentation 失效
    通过发酵生产L-赖氨酸和L-谷氨酸的方法

    公开(公告)号:US5846790A

    公开(公告)日:1998-12-08

    申请号:US776597

    申请日:1997-02-18

    摘要: A mutant strain having an ability to produce L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to produce and accumulate L-glutamic acid in the medium. A mutant strain having an ability to produce L-lysine and L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent and giving L-lysine productivity to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to simultaneously produce and accumulate L-lysine and L-glutamic acid in the medium.

    摘要翻译: PCT No.PCT / JP95 / 01586 Sec。 371日期1997年2月18日 102(e)1997年2月18日PCT PCT 1995年8月9日PCT公布。 出版物WO96 / 06180 日期:1996年2月29日在具有生物素活性抑制剂的情况下,在含有过量生物素的培养基中,不存在生成L-谷氨酸的能力的突变株是通过赋予生物素作用抑制 代谢产L-谷氨酸棒状细菌。 将该菌株在液体培养基中培养以在培养基中产生并积累L-谷氨酸。 通过赋予生物素活性抑制剂的温度敏感度,获得在含有过量生物素的培养基中不存在任何生物素活性抑制剂时能够产生L-赖氨酸和L-谷氨酸的突变株 并向L-谷氨酸棒状杆菌产生细菌提供L-赖氨酸生产力。 该菌株在液体培养基中培养,以在培养基中同时产生和积累L-赖氨酸和L-谷氨酸。

    Stress-resistant microorganism and method of producing fermentative product
    7.
    发明授权
    Stress-resistant microorganism and method of producing fermentative product 有权
    耐应力微生物和发酵产物的生产方法

    公开(公告)号:US06338956B1

    公开(公告)日:2002-01-15

    申请号:US09634632

    申请日:2000-08-08

    IPC分类号: C12P1304

    摘要: A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a &sgr; factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.

    摘要翻译: 利用微生物通过在培养基中培养微生物发酵生产有用物质如氨基酸,使发酵产物在培养基中产生和积累,收集发酵产物,其中所用的微生物通过引入进行修饰 编码热休克蛋白的基因中的至少一种和编码特异性用于热休克蛋白基因的西格玛因子的基因以增强细胞中的热休克蛋白的表达量,由此允许微生物添加 否则会抑制微生物的生长和/或发酵产物的产生。

    Stress-resistant microorganism and method of producing fermentative
product
    10.
    发明授权
    Stress-resistant microorganism and method of producing fermentative product 失效
    耐应力微生物和发酵产物的生产方法

    公开(公告)号:US6156532A

    公开(公告)日:2000-12-05

    申请号:US894223

    申请日:1997-08-25

    摘要: A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a .sigma. factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.

    摘要翻译: PCT No.PCT / JP96 / 00287 Sec。 371日期1997年8月25日第 102(e)日期1997年8月25日PCT提交1996年2月9日PCT公布。 公开号WO96 / 26289 日期1996年8月29日利用微生物通过在培养基中培养微生物发酵生产有用物质如氨基酸,以使发酵产物在培养基中产生并积累,并收集发酵产物,其中微生物为 通过引入编码热休克蛋白的基因和编码特异性用于热休克蛋白基因的σ因子的基因中的至少一种来修饰,以增强细胞中热休克蛋白的表达量,由此微生物 被允许增加抗应力,否则会抑制微生物的生长和/或发酵产物的产生。