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72 条记录 (耗时 0.068 秒)

    Methods and Assays for Capture of Nucleic Acids
    41.
    发明申请
    Methods and Assays for Capture of Nucleic Acids 审中-公开
    捕获核酸的方法和测定

    公开(公告)号：US20090246788A1

    公开(公告)日：2009-10-01

    申请号：US12408485

    申请日：2009-03-20

    申请人： Thomas Albert ,  Jigar Patel ,  Victor Lyamichev

    发明人： Thomas Albert ,  Jigar Patel ,  Victor Lyamichev

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6837 ,  C12Q2525/301 ,  C12Q2521/501 ,  C12Q2521/301 ,  C12Q2561/109

    摘要： The present disclosure provides methods and systems for sequence specific nucleic acid target capture comprising enzymatic reactions. The present disclosure relates to a plurality of oligonucleotide probes for capture and subsequent detection of target nucleic acid sequences, using flap endonucleases, ligases, and/or additional enzymes, proteins or compounds, on substrates, for example microarray slides, and in solution formats.

    摘要翻译： 本公开提供了包含酶反应的序列特异性核酸靶捕获的方法和系统。 本公开涉及用于捕获和随后检测靶核酸序列的多个寡核苷酸探针，其在底物（例如微阵列载玻片）上以溶液形式，使用翼内切核酸酶，连接酶和/或其它酶，蛋白质或化合物。

    Detection of allelic expression imbalance
    42.
    发明申请
    Detection of allelic expression imbalance 审中-公开
    检测等位基因表达不平衡

    公开(公告)号：US20060199202A1

    公开(公告)日：2006-09-07

    申请号：US11349872

    申请日：2006-02-08

    申请人： Victor Lyamichev ,  Hatim Allawi

    发明人： Victor Lyamichev ,  Hatim Allawi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2561/109 ,  C12Q2545/114

    摘要： The present invention provides compositions and methods for the detection and characterization of allelic expression imbalance from a heterozygous gene locus. More particularly, the present invention provides compositions, kits, and methods for the determination of allelic expression imbalance by the comparison of expression levels from each of two alleles of a given gene locus through the use of an invasive cleavage structure assay (e.g. the INVADER assay).

    摘要翻译： 本发明提供了用于检测和表征杂合基因座的等位基因表达不平衡的组合物和方法。 更具体地，本发明通过使用侵入性切割结构测定法（例如INVADER测定法）来比较来自给定基因位点的两个等位基因中的每一个的表达水平来确定等位基因表达失衡的组合物，试剂盒和方法 ）。

    Detection of nuceic acids by multiple sequential invasive cleavages
    44.
    发明申请
    Detection of nuceic acids by multiple sequential invasive cleavages 有权
    通过多次连续侵入性裂解检测酸性酸

    公开(公告)号：US20050003432A1

    公开(公告)日：2005-01-06

    申请号：US10897793

    申请日：2004-07-23

    申请人： Jeff Hall ,  Victor Lyamichev ,  Andrea Mast ,  Mary Brow ,  Robert Kwiatkowski ,  Stephanie Vavra

    发明人： Jeff Hall ,  Victor Lyamichev ,  Andrea Mast ,  Mary Brow ,  Robert Kwiatkowski ,  Stephanie Vavra

    IPC分类号： C12N9/12 ,  C12N9/22 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12N9/1252 ,  C12N9/22 ,  C12Q1/6823 ,  C12Q1/683 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q1/706 ,  Y10S435/81 ,  C12Q2561/109

    摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.

    摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中各种病毒的核酸的敏感和具体方法。

    Nucleic acid cleavage assays
    45.
    发明申请
    Nucleic acid cleavage assays 审中-公开
    核酸切割测定

    公开(公告)号：US20070292856A1

    公开(公告)日：2007-12-20

    申请号：US11489665

    申请日：2006-07-19

    申请人： Victor Lyamichev ,  Michael Kaiser ,  Natalie Lyamichev ,  Jeff Hall ,  James Prudent ,  Mary Brow

    发明人： Victor Lyamichev ,  Michael Kaiser ,  Natalie Lyamichev ,  Jeff Hall ,  James Prudent ,  Mary Brow

    IPC分类号： C12Q1/68

    CPC分类号： C12N9/22 ,  C12Q1/6813 ,  C12Q2521/301

    摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

    摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。