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    LINKER COMPOSITION COMPRISING SILICA-FORMING PEPTIDES
    51.
    发明申请
    LINKER COMPOSITION COMPRISING SILICA-FORMING PEPTIDES 审中-公开

    公开(公告)号：US20200261593A1

    公开(公告)日：2020-08-20

    申请号：US16793081

    申请日：2020-02-18

    申请人： Korea University Research and Business Foundation, Sejong Campus

    发明人： Seung Pil PACK ,  Mohamed Abdeltawab Abdallah ABDELHAMID ,  Ki Baek YEO ,  Mi Ran KI

    IPC分类号： A61K47/65 ,  A61K47/64

    摘要： The present disclosure relates to the use of silica-forming peptides (SFPs) Volp1 and Salp1 for protein fusion tags for protein purification, silica formation and self-encapsulation, and controlled release of biomolecules. After preparing a fusion protein containing the Volp1 or Salp1 peptide at the C-terminal of the protein, the adsorption conditions for silica (SiO2) surface were optimized. As a result, a high-purity fusion protein could be purified without an additional tag for purification. The fusion protein containing the silica-forming peptide was self-encapsulated and stably immobilized in the silica matrix through reaction with a silica precursor. The Volp1 and Salp1 peptides showed stronger adsorption ability for silica than the previously known R5 peptide. Using these characteristics, a silica complex wherein two proteins are encapsulated was prepared by mixing the R5 fusion protein with the Volp1 or Salp1 fusion protein. The resulting controlled release system allows the release of the R5 fusion protein in the first step and release of the Salp1 fusion protein in the second step based on the difference in adsorption affinity.

    High-speed and low-power pipelined ADC using dynamic reference voltage and 2-stage sample-and-hold
    53.
    发明授权
    High-speed and low-power pipelined ADC using dynamic reference voltage and 2-stage sample-and-hold 有权

    公开(公告)号：US10411722B2

    公开(公告)日：2019-09-10

    申请号：US16168336

    申请日：2018-10-23

    申请人： Korea University Research and Business Foundation, Sejong Campus

    发明人： Hayun Cecillia Chung

    IPC分类号： H03M1/12 ,  H03M1/16 ,  H03M1/46

    摘要： Disclosed is a high-speed and low-power pipelined analog-digital converter (ADC) using a dynamic reference voltage and a 2-stage S/H. The pipelined ADC includes a 2-stage sample-and-hold (S/H) configured to secure a conversion time corresponding to a clock cycle per stage and to apply only a buffer to an input signal path, a reference voltage generator configured to receive the output of the D flip-flop of a previous stage as an input signal and to generate a required reference voltage during a half cycle of a sample frequency, and a comparator configured to include a linear transconductor (LT), a rail-to-rail latch (R2R) and a D flip-flop and to generate the output of the ADC and input to the reference voltage generator of a next stage for generating a reference voltage using the output of the D flip-flop.

    Method for preparing DNA oligomer into which single nucleotide is incorporated using terminal deoxynucelotidyl transferase
    58.
    发明授权
    Method for preparing DNA oligomer into which single nucleotide is incorporated using terminal deoxynucelotidyl transferase 有权

    公开(公告)号：US11584952B2

    公开(公告)日：2023-02-21

    申请号：US16758523

    申请日：2018-10-12

    申请人： Korea University Research and Business Foundation, Sejong Campus

    发明人： Seung Pil Pack ,  Eui Kyoung Jang

    IPC分类号： C12Q1/6806

    摘要： The present invention relates to a method for preparing a DNA oligomer into which a single nucleotide is incorporated using a terminal deoxynucleotidyl transferase. According to the present invention, by using a base hydrolysis reaction or a ribose-borate complex formation method, single incorporation of normal and modified nucleotides in a TdT enzyme-based DNA oligomer modification method can be facilitated. In addition, the method simultaneously provides the usability of TdT and the quantitativeness of modification group incorporation, thereby being effectively usable in the development of a quantitative detection technique or in a crosslinking reaction, both of which require quantitativeness and, according to the present invention, a DNA oligomer, into which a single nucleotide which is a product of a TdT reaction is incorporated, has an exposed a 3′ hydroxyl terminus, which is an enzyme recognition site, such that an additional enzymatic technique such as primer extension or ligation can be introduced.