公开(公告)号：US07871789B2

公开(公告)日：2011-01-18

申请号：US12084003

申请日：2006-10-27

申请人： Satoshi Yonehara ,  Norio Imamura

发明人： Satoshi Yonehara ,  Norio Imamura

IPC分类号： C12Q1/37

CPC分类号： C12Q1/37 ,  G01N33/68 ,  G01N2333/76 ,  G01N2400/00

摘要： An albumin denaturing agent for digesting an albumin by a protease efficiently is provided. The albumin denaturing agent contains quaternary ammonium having a hydrocarbon group with a carbon number of 12 or more, or a salt of the quaternary ammonium. The albumin in a sample is digested by the protease in the presence of the albumin denaturing agent, a glycated part of the thus obtained albumin digestion product and a FAOD effect a reaction, and a redox reaction between the glycated part and the FAOD is measured, thereby determining a ratio (GA (%)) of the glycated albumin of the glycated albumin with respect to the albumin.

摘要翻译： 提供了通过蛋白酶有效地消化白蛋白的白蛋白变性剂。 白蛋白变性剂含有碳数为12以上的烃基的季铵或季铵盐。 在白蛋白变性剂存在下，将蛋白质消化，由此得到的白蛋白消化产物的糖化部分和FAOD进行反应，测定糖化部分与FAOD之间的氧化还原反应， 从而确定糖化白蛋白的糖化白蛋白相对于白蛋白的比率（GA（％））。

公开(公告)号：US20100190194A1

公开(公告)日：2010-07-29

申请号：US12376914

申请日：2007-08-10

申请人： Satoshi Yonehara ,  Toshihiro Imai

发明人： Satoshi Yonehara ,  Toshihiro Imai

IPC分类号： C12Q1/34 ,  C07K14/435 ,  G01N33/72 ,  C12Q1/00 ,  C12Q1/02

CPC分类号： G01N33/723 ,  G01N27/447 ,  G01N2030/027 ,  G01N2333/906 ,  G01N2800/042

摘要： A new method of diagnosing postprandial hyperglycemia by indirectly measuring a blood glucose level is provided. Postprandial hyperglycemia is detected by measuring a glycation degree of lysine in hemoglobin, in which a side chain amino group of lysine is glycated (GHbLys %). Measurement of GHbLys % can be performed by cleaving hemoglobin by protease, treating a glycated part of a lysine residue in the obtained cleavage product of hemoglobin with fructosyl amino acid oxidase, and measuring a redox reaction between the glycated part and fructosyl amino acid oxidase.

摘要翻译： 提供了一种通过间接测量血糖水平来诊断餐后高血糖的新方法。 通过测定血红蛋白中的赖氨酸的糖基化程度，其中赖氨酸的侧链氨基被糖化（GHbLys％）来检测餐后高血糖。 GHbLys％的测定可以通过蛋白酶切割血红蛋白，用获得的果糖基氨基酸氧化酶处理得到的血红蛋白裂解产物的赖氨酸残基的糖基化部分，测定糖基化部分和果糖基氨基酸氧化酶之间的氧化还原反应。

公开(公告)号：US20100032297A1

公开(公告)日：2010-02-11

申请号：US12514301

申请日：2008-04-23

申请人： Koji Sugiyama ,  Satoshi Yonehara ,  Yusuke Nakayama

发明人： Koji Sugiyama ,  Satoshi Yonehara ,  Yusuke Nakayama

IPC分类号： G01N27/447

CPC分类号： G01N27/44791 ,  B01L3/50273 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2400/0421 ,  C07K1/26

摘要： An electrophoresis chip that enables an apparatus to be small, analysis time to be short and glycosylated hemoglobin to be analyzed highly accurately is provided. The electrophoresis chip includes an upper substrate 4, a lower substrate 1, a first introduction reservoir 2a, a first recovery reservoir 2b and a capillary channel for sample analysis 3x; the first introduction reservoir 2a and the first recovery reservoir 2b are formed in the lower substrate 1; and the first introduction reservoir 2a and the first recovery reservoir 2b are in communication with each other via the capillary channel for sample analysis 3x.

摘要翻译： 提供能够使装置小型，分析时间短并能够高精度地分析糖基化血红蛋白的电泳芯片。 电泳芯片包括上基板4，下基板1，第一引入槽2a，第一回收槽2b和用于样品分析的毛细通道3x; 第一引入槽2a和第一回收槽2b形成在下基板1中; 并且第一引入槽2a和第一回收槽2b经由用于样品分析3x的毛细通道彼此连通。

公开(公告)号：US20100032294A1

公开(公告)日：2010-02-11

申请号：US12514293

申请日：2008-04-23

申请人： Yusuke Nakayama ,  Satoshi Yonehara

发明人： Yusuke Nakayama ,  Satoshi Yonehara

IPC分类号： G01N27/447 ,  C09K3/00

CPC分类号： G01N27/44747

摘要： The present invention provides a method for analyzing hemoglobin by capillary electrophoresis, that allows the apparatus to be smaller in size, allows a highly precise analysis to be obtained, and allows the analysis to be performed in a short period of time. The analytical method of the present invention are methods for analyzing hemoglobin by capillary electrophoresis, comprising: a sample-providing step of providing a sample containing hemoglobin; a capillary tube-providing step of providing a capillary tube containing a buffer solution; and an electrophoresis step of carrying out electrophoresis of the sample, by introducing the sample into the buffer solution in the capillary tube, and applying a voltage across both ends of the capillary tube; wherein the electrophoresis is carried out following at least one of modes (A) and (B) below: (A) the electrophoresis is carried out with a surfactant (a) added to the buffer solution, the surfactant (a) being a non-ionic surfactant having an alkyl group as a hydrophobic portion and a sugar as a hydrophilic portion; and (B) the electrophoresis is carried out with a surfactant (b) added to the sample, the surfactant (b) being a betaine-type amphoteric surfactant.

摘要翻译： 本发明提供了一种通过毛细管电泳分析血红蛋白的方法，其允许装置尺寸更小，可以获得高精度分析，并允许在短时间内进行分析。 本发明的分析方法是通过毛细管电泳分析血红蛋白的方法，包括：提供含有血红蛋白的样品的样品提供步骤; 毛细管提供步骤，提供含有缓冲溶液的毛细管; 以及电泳步骤，通过将样品引入毛细管中的缓冲溶液中并在毛细管的两端施加电压，进行样品的电泳; 其中所述电泳按照以下模式（A）和（B）中的至少一种进行：（A）用添加到缓冲溶液中的表面活性剂（a）进行电泳，所述表面活性剂（a） 具有作为疏水部分的烷基和作为亲水部分的糖的离子表面活性剂; （B）用表面活性剂（b）进行电泳，表面活性剂（b）为甜菜碱型两性表面活性剂。

公开(公告)号：US20090215026A1

公开(公告)日：2009-08-27

申请号：US12416507

申请日：2009-04-01

申请人： Kaori ISHIMARU ,  Satoshi YONEHARA

发明人： Kaori ISHIMARU ,  Satoshi YONEHARA

IPC分类号： C12Q1/00 ,  C12N9/48

CPC分类号： C07D257/04 ,  C07B63/04

摘要： A method for storing a tetrazolium compound stably is provided. The tetrazolium compound is stored in the presence of sodium azide. The tetrazolium compound (A) and the sodium azide (B) are present at a ratio (A:B) in the range from 1:0.02 to 1:6.2. Furthermore, when the tetrazolium compound is stored as a solution, the concentration of the sodium azide is in the range from 0.08 to 3.2 mmol/L and the concentration of the tetrazolium compound is in the range from 0.5 to 8 mmol/L. As the tetrazolium compound, it is preferable to use 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt.

摘要翻译： 提供稳定地存储四唑化合物的方法。 四唑化合物在叠氮化钠存在下储存。 四氮唑化合物（A）和叠氮化钠（B）的比例（A:B）为1:0.02至1:6.2。 此外，当四唑鎓化合物作为溶液储存时，叠氮化钠的浓度在0.08-3.2mmol / L的范围内，四唑鎓化合物的浓度在0.5-8mmol / L的范围内。 作为四唑鎓化合物，优选使用2-（4-碘苯基）-3-（2,4-二硝基苯基）-5-（2,4-二磺基苯基）-2H-四唑鎓盐。

公开(公告)号：US07432072B2

公开(公告)日：2008-10-07

申请号：US10514382

申请日：2003-05-02

申请人： Satoshi Yonehara ,  Tsuguki Komori

发明人： Satoshi Yonehara ,  Tsuguki Komori

IPC分类号： C12Q1/26 ,  C07D257/00

CPC分类号： C12Q1/26

摘要： The present invention provides a method of preventing erroneous color development of N-(carboxymethylaminocarbonyl)-4,4′-bis(dimethylamino)diphenylamine sodium salt as a color-developing substrate, thereby improving the accuracy of measurement utilizing a redox reaction performed using the color-developing substrate. A tetrazolium compound, sodium azide, and the color-developing substrate are added to a sample in the presence of a surfactant. A reaction between an oxidizing substance derived from an analyte in the sample and the color-developing substrate, which develops color by oxidation, is caused by an oxidoreductase. By measuring the color developed, the amount of the oxidizing substance is determined. The concentrations of the respective components in the reaction solution are set so that 0.01 to 1 mmol of the tetrazolium compound, 0.003 to 0.5 mmol of the sodium azide, and 0.006 to 0.4 mmol of the surfactant are present per μmol of the color-developing substrate, and the pH of the reaction solution is set in the range from 6 to 9.

摘要翻译： 本发明提供了防止N-（羧甲基氨基羰基）-4,4'-双（二甲基氨基）二苯胺钠盐作为显色底物错误显色的方法，从而通过使用 彩色显影基板。 在表面活性剂存在下，将四唑化合物，叠氮化钠和显色底物加入到样品中。 衍生自样品中的分析物的氧化物质与由氧化形成颜色的显色底物之间的反应是由氧化还原酶引起的。 通过测量显色，确定氧化物质的量。 将反应溶液中各成分的浓度设定为使每一个显色基质的每一个都存在0.01-1mmol四唑化合物，0.003-0.5mmol叠氮化钠和0.006-0.4mmol表面活性剂 ，反应溶液的pH设定在6〜9的范围内。

公开(公告)号：US07381539B2

公开(公告)日：2008-06-03

申请号：US10515715

申请日：2003-04-28

申请人： Satoshi Yonehara ,  Kaori Ishimaru

发明人： Satoshi Yonehara ,  Kaori Ishimaru

IPC分类号： C12Q1/26

CPC分类号： C12Q1/26 ,  G01N33/52 ,  G01N2400/00

摘要： The present invention provides a highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a formazan compound is added to a sample prior to a redox reaction so as to eliminate the influence of any reducing substance in the sample. Thereafter, a reducing substance or an oxidizing substance derived from the analyte is formed, and the amount of the formed substance is measured by the redox reaction. The amount of the analyte is determined from the amount of the formed substance thus measured. As the formazan compound, for example, 1-(4-iodophenyl)-3-(2,4-disulfophenyl)-5-(2,4-dinitrophenyl) formazan can be used.

摘要翻译： 本发明提供了使用氧化还原反应测量样品中分析物的高度可靠的方法。 在该方法中，在氧化还原反应之前，将样品化合物加入到样品中，以消除样品中任何还原物质的影响。 此后，形成来自分析物的还原物质或氧化物质，通过氧化还原反应测定形成物质的量。 分析物的量由所测量的形成物质的量确定。 作为甲an化合物，例如可以使用1-（4-碘苯基）-3-（2,4-二磺基苯基）-5-（2,4-二硝基苯基）甲。。

公开(公告)号：US07354732B2

公开(公告)日：2008-04-08

申请号：US10517853

申请日：2003-04-28

申请人： Satoshi Yonehara ,  Kaori Ishimaru ,  Kaoru Hirai

发明人： Satoshi Yonehara ,  Kaori Ishimaru ,  Kaoru Hirai

IPC分类号： C12Q1/26 ,  C12Q1/28

CPC分类号： C12Q1/28 ,  C12Q1/26 ,  G01N33/72

摘要： A method for measuring an analyte in a sample by using a redox reaction is provided, which gives values with excellent reliability. Prior to the redox reaction, at least one of a sulfonic acid compound and a nitro compound is added to the sample to eliminate the influence of hemoglobin and any hemoglobin degradation products as reducing substances contained in the sample. Subsequently, a reducing or oxidizing substance derived from the analyte is caused to generate, and the amount thereof is measured by the redox reaction. The amount of the analyte is determined from the measurement value. The sulfonic acid compound may be sodium lauryl sulfate, and the nitro compound may be 4-nitrophenol, etc.

摘要翻译： 提供了通过使用氧化还原反应来测量样品中的分析物的方法，其给出具有优异可靠性的值。 在氧化还原反应之前，将至少一种磺酸化合物和硝基化合物加入到样品中以消除血红蛋白和任何血红蛋白降解产物作为样品中所含的还原物质的影响。 随后，产生来自分析物的还原性或氧化性物质，并且通过氧化还原反应测量其量。 从测量值确定分析物的量。 磺酸化合物可以是十二烷基硫酸钠，硝基化合物可以是4-硝基苯酚等。

公开(公告)号：US07235378B2

公开(公告)日：2007-06-26

申请号：US10332790

申请日：2001-07-12

申请人： Satoshi Yonehara

发明人： Satoshi Yonehara

IPC分类号： C12Q1/54

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N33/723 ,  G01N33/725

摘要： A method of determining glycated hemoglobin is provided, by which a ratio of the glycated hemoglobin in a sample can be determined accurately and easily. The ratio of glycated hemoglobin can be determined by degrading a glycated hemoglobin in a whole blood sample selectively with a protease to give a glycated hemoglobin degradation product; causing a redox reaction between a glycation site of the glycated hemoglobin degradation product and a fructosyl amino acid oxidoreductase; and determining this redox reaction. Further, as shown in FIG. 1, in a whole blood sample, there is a correlation between the ratio of the glycated hemoglobin determined by this method and an HbA1c concentration. Thus, without determining the glycated α-amino group as a characteristic structure of HbA1c, an amount of HbA1c can be determined accurately and easily from the determined ratio of the glycated hemoglobin.

摘要翻译： 提供了测定糖化血红蛋白的方法，通过该方法可以准确且容易地测定样品中糖化血红蛋白的比例。 糖化血红蛋白的比例可以通过用蛋白酶选择性降解全血样品中的糖化血红蛋白来得到糖化血红蛋白降解产物; 引起糖化血红蛋白降解产物的糖化位点与果糖基氨基酸氧化还原酶之间的氧化还原反应; 并确定这种氧化还原反应。 此外，如图1所示。 如图1所示，在全血样品中，通过该方法测定的糖化血红蛋白的比例与HbA1c浓度存在相关性。 因此，在不确定糖化α-氨基作为HbA1c的特征结构的情况下，可以从测定的糖化血红蛋白的比例准确且容易地测定HbA1c的量。

公开(公告)号：US20060172367A1

公开(公告)日：2006-08-03

申请号：US10528992

申请日：2003-09-16

申请人： Nobuyuki Yoshida ,  Yoshiki Tani ,  Satoshi Yonehara

发明人： Nobuyuki Yoshida ,  Yoshiki Tani ,  Satoshi Yonehara

IPC分类号： C12Q1/30 ,  C07H21/04 ,  C12P21/06 ,  C12N9/06 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N9/0022 ,  C12Q1/26

摘要： The present invention provides a fructosylamine oxidase which is obtainable by culturing Fusarium proliferatum, and purifying two types of fructosylamine oxidase (FAO) with different substrate specificities from the culture, and which is useful in the measurement of amadori compounds.

摘要翻译： 本发明提供了可通过培养镰刀菌（Fusarium proliferatum）获得的果糖胺氧化酶，并从培养物中纯化具有不同底物特异性的两种类型的果糖胺氧化酶（FAO），其可用于测量阿马多利化合物。