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公开(公告)号：US07429455B2
公开(公告)日：2008-09-30
申请号：US11515854
申请日：2006-09-05
申请人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
发明人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
CPC分类号： C12Q1/6832 , C12Q1/701 , C12Q2565/133 , C12Q2521/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US07067643B2
公开(公告)日：2006-06-27
申请号：US09941193
申请日：2001-08-28
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N9/1252 , C12N15/10 , C12Q1/68 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , C12Q1/6853 , C12Q1/686 , C12Q1/689 , C12Q1/705 , Y10T436/143333 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
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公开(公告)号：US07011944B2
公开(公告)日：2006-03-14
申请号：US09982667
申请日：2001-10-18
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US06780585B1
公开(公告)日：2004-08-24
申请号：US09676768
申请日：2000-10-02
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2565/133 , C12Q2565/501
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US06759226B1
公开(公告)日：2004-07-06
申请号：US09577304
申请日：2000-05-24
申请人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
发明人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
IPC分类号： C12N922
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.
摘要翻译： 本发明涉及设计用于直接检测，表征和定量核酸，特别是RNA的新型酶。 本发明提供了识别在靶RNA序列上形成的特异性核酸切割结构并以位点特异性方式切割核酸切割结构以产生非靶切割产物的酶。 本发明提供具有改进的能够特异性切割包含DNA和RNA核酸链的复合物的DNA成员的酶的酶。
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公开(公告)号：US06635463B2
公开(公告)日：2003-10-21
申请号：US09758282
申请日：2001-01-11
申请人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
发明人： Wu-Po Ma , Victor I. Lyamichev , Michael W. Kaiser , Natalie E. Lyamicheva , Hatim Taysir Allawi , James J. Schaefer , Bruce P. Neri
IPC分类号： C12N922
CPC分类号： C12Q1/6823 , C12N9/1252 , C12N9/22 , C12Q1/683 , Y10T436/143333 , C12Q2561/109
摘要： The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.
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公开(公告)号：US6090606A
公开(公告)日：2000-07-18
申请号：US758314
申请日：1996-12-02
CPC分类号： C12Q1/6823 , C12N9/22 , C12Q1/683
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to improved cleavage means for the detection and characterization of nucleic acid sequences. Structure-specific nucleases derived from a variety of thermostabe organisms are provided. These structure-specific nucleases are used to cleave target-dependent cleavage structures, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及用于检测和表征核酸序列的改进的切割装置。 提供了衍生自各种恒温器生物体的结构特异性核酸酶。 这些结构特异性核酸酶用于切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US5985557A
公开(公告)日：1999-11-16
申请号：US756386
申请日：1996-11-26
IPC分类号： G01N33/50 , C12N1/21 , C12N9/12 , C12N9/16 , C12N9/22 , C12N15/09 , C12Q1/68 , G01N33/566 , C07H21/02
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US5871911A
公开(公告)日：1999-02-16
申请号：US385629
申请日：1995-02-09
IPC分类号： C07H21/00 , C07K14/47 , C12N9/12 , C12N15/09 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , G01N33/53 , C07H21/04 , C12N15/00 , C12P19/34
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C12N15/10 , C12N9/1252 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/6853 , C12Q1/686 , C12Q1/705
摘要： A method of cleaving a target nucleic acid molecule is disclosed. A cleavage structure is formed that comprises the target nucleic acid and a pilot nucleic acid. A first region of the target nucleic acid is annealed to the pilot nucleic acid to form a duplex structure. A second region of the target nucleic acid contiguous to the duplex is not annealed to the pilot nucleic acid, thus forming a junction site between the duplex region and the non-annealed region. The cleavage structure is exposed to a cleavage agent capable of preferentially cleaving the cleavage structure at a target site in a manner independent of the sequence of the cleavage structure. The cleavage structure and the cleavage agent are incubated under conditions wherein cleavage can occur.
摘要翻译： 公开了切割靶核酸分子的方法。 形成包含靶核酸和导向核酸的切割结构。 靶核酸的第一区域与引导核酸退火以形成双链体结构。 与双链体相邻的靶核酸的第二区域不与引导核酸退火，从而在双相区域和非退火区域之间形成连接位点。 裂解结构暴露于能够以与切割结构的序列无关的方式在靶位点优先切割切割结构的切割剂。 裂解结构和切割剂在可以发生切割的条件下孵育。
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