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公开(公告)号：US09353405B2
公开(公告)日：2016-05-31
申请号：US13436174
申请日：2012-03-30
申请人： Elazar Rabbani , James J. Donegan , Dakai Liu
发明人： Elazar Rabbani , James J. Donegan , Dakai Liu
CPC分类号： C12Q1/6818 , C12Q1/6844 , C12Q1/6846 , C12Q1/6853 , C12Q1/6876 , C12Q1/707 , C12Q1/708 , C12Q2565/101 , C12Q2533/101
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided. Paneling and multiplex analyzes of more than one nucleic acid analyte using one sample are also provided.
摘要翻译： 本发明提供用于实时核酸检测过程的组合物。 这种实时核酸检测方法是用连接于核酸引物，核苷酸，核酸探针或核酸结合剂的能量转移元件进行的。 实时核酸检测允许定性或定量检测或测定样品中感兴趣的单链或双链核酸。 本发明提供了其它方法，包括从被分析物或分析物库中除去一部分均聚物序列，例如聚A序列或尾部的方法。 还描述和提供了用于进行这种去除方法的组合物。 还提供了使用一个样品的多于一种核酸分析物的镶板和多重分析。
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52.发明授权Method for treating inflammation by administering a compound which binds LDL-receptor-related protein (LRP) ligand binding domain 有权通过施用结合LDL-受体相关蛋白（LRP）配体结合结构域的化合物来治疗炎症的方法公开(公告)号：US09046537B2
公开(公告)日：2015-06-02
申请号：US12221863
申请日：2008-08-07
申请人： Elazar Rabbani , Xiaofeng Li , Dakai Liu , Yazhou Zhang , Richard Jin , Riddhi Bhattacharyya , Wei Cheng , James J. Donegan
发明人： Elazar Rabbani , Xiaofeng Li , Dakai Liu , Yazhou Zhang , Richard Jin , Riddhi Bhattacharyya , Wei Cheng , James J. Donegan
IPC分类号： A01N43/00 , A61K31/33 , A01N61/00 , A61K31/00 , A61K31/535 , A01N43/02 , A61K31/335 , A01N43/16 , A61K31/35 , G01N33/92 , A61K31/015 , A61K31/395 , G01N33/50 , G01N33/564 , A61K38/00
CPC分类号： A61K31/538 , A61K31/015 , A61K31/185 , A61K31/192 , A61K31/235 , A61K31/395 , A61K31/535 , A61K38/00 , G01N33/5011 , G01N33/5041 , G01N33/564 , G01N33/92 , G01N2333/705 , G01N2800/24
摘要： The present invention relates to the field of therapeutic methods, compositions and uses thereof, that affect, directly or indirectly, the behavior of LRP receptors. These compositions and methods result in the treatment of inflammatory, immunological and metabolic conditions. More particularly, the methods and compositions of the invention are directed to the identification of small molecules, drugs and/or pharmacological agents that affect the Wnt pathway by affecting normal complex formation among various signaling receptors, the LRP5 and LRP6 receptor, and related ligands.
摘要翻译： 本发明涉及直接或间接影响LRP受体的行为的治疗方法，组合物和用途领域。 这些组合物和方法导致治疗炎症，免疫和代谢疾病。 更具体地，本发明的方法和组合物涉及通过影响各种信号传导受体（LRP5和LRP6受体）和相关配体之间的正常复合物形成来鉴定影响Wnt途径的小分子，药物和/或药理学试剂。
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53.发明申请公开(公告)号：US20060024735A1
公开(公告)日：2006-02-02
申请号：US11236151
申请日：2005-09-27
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
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54.发明申请公开(公告)号：US20060029968A1
公开(公告)日：2006-02-09
申请号：US11235516
申请日：2005-09-26
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
摘要翻译： 本发明提供用于实时核酸检测过程的组合物。 这种实时核酸检测方法是用连接于核酸引物，核苷酸，核酸探针或核酸结合剂的能量转移元件进行的。 实时核酸检测允许定性或定量检测或测定样品中感兴趣的单链或双链核酸。 本发明提供了其它方法，包括从被分析物或分析物库中除去一部分均聚物序列，例如聚A序列或尾部的方法。 还描述和提供了用于进行这种去除方法的组合物。
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公开(公告)号：US20050137388A1
公开(公告)日：2005-06-23
申请号：US10096076
申请日：2002-03-12
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
摘要翻译： 本发明提供用于实时核酸检测过程的组合物。 这种实时核酸检测方法是用连接于核酸引物，核苷酸，核酸探针或核酸结合剂的能量转移元件进行的。 实时核酸检测允许定性或定量检测或测定样品中感兴趣的单链或双链核酸。 本发明提供了其它方法，包括从被分析物或分析物库中除去一部分均聚物序列，例如聚A序列或尾部的方法。 还描述和提供了用于进行这种去除方法的组合物。
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公开(公告)号：US08445664B2
公开(公告)日：2013-05-21
申请号：US11256592
申请日：2005-10-20
IPC分类号： C07H21/04
CPC分类号： C12Q1/6844 , C12Q1/6853 , C12Q2531/119 , C12Q2525/301 , C12Q2525/161
摘要： This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic acid construct is utilized to carry out the amplification process. In non-linear amplification, a first initial primer or nucleic acid construct is employed with a subsequent initial primer or nucleic acid construct. In other non-linear amplification processes provided by this invention, a first initial primer or nucleic acid construct is deployed with a second initial primer or nucleic acid construct to amplify the specific nucleic acid sequence of interest and its complement that are provided. A singular primer or a singular nucleic acid construct capable of non-linear amplification can also be used to carry out non-linear amplification in accordance with this invention. Post-termination labeling process for nucleic acid sequencing is also disclosed in this invention that is based upon the detection of tagged molecules that are covalently bound to chemically reactive groups provided for chain terminators. A process for producing nucleic acid sequences having decreased thermodynamic stability to complementary sequences is also provided and achieved by this invention. Unique nucleic acid polymers are also disclosed and provided in addition to other novel compositions, kits and the like.
摘要翻译： 本发明提供用于扩增感兴趣的核酸序列的新方法，包括线性和非线性扩增。 在线性扩增中，使用单个初始引物或核酸构建体进行扩增过程。 在非线性扩增中，使用第一初始引物或核酸构建体与随后的初始引物或核酸构建体。 在本发明提供的其它非线性扩增方法中，用第二初始引物或核酸构建物展开第一个初始引物或核酸构建体，以扩增所提供的特异性核酸序列及其互补序列。 也可以使用能够进行非线性扩增的单一引物或单个核酸构建体来进行本发明的非线性扩增。 用于核酸测序的终止后标记方法也在本发明中公开，其基于共价结合到为链终止剂提供的化学反应性基团的标记分子的检测。 通过本发明提供并实现了对互补序列产生具有降低的热力学稳定性的核酸序列的方法。 除了其它新型组合物，试剂盒等之外，还公开并提供了独特的核酸聚合物。
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57.发明申请Processes for incorporating nucleic acid sequences into an analyte or library of analytes 有权将核酸序列并入分析物或分析物库中的方法公开(公告)号：US20060035264A1
公开(公告)日：2006-02-16
申请号：US11237466
申请日：2005-09-27
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
摘要翻译： 本发明提供用于实时核酸检测过程的组合物。 这种实时核酸检测方法是用连接于核酸引物，核苷酸，核酸探针或核酸结合剂的能量转移元件进行的。 实时核酸检测允许定性或定量检测或测定样品中感兴趣的单链或双链核酸。 本发明提供了其它方法，包括从被分析物或分析物库中除去一部分均聚物序列，例如聚A序列或尾部的方法。 还描述和提供了用于进行这种去除方法的组合物。
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58.发明申请Site- or sequence-specific process for cleaving analytes and library of analytes 有权用于切割分析物和分析物库的位点或序列特异性方法公开(公告)号：US20060024738A1
公开(公告)日：2006-02-02
申请号：US11237467
申请日：2005-09-27
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
摘要翻译： 本发明提供用于实时核酸检测过程的组合物。 这种实时核酸检测方法是用连接于核酸引物，核苷酸，核酸探针或核酸结合剂的能量转移元件进行的。 实时核酸检测允许定性或定量检测或测定样品中感兴趣的单链或双链核酸。 本发明提供了其它方法，包括从被分析物或分析物库中除去一部分均聚物序列，例如聚A序列或尾部的方法。 还描述和提供了用于进行这种去除方法的组合物。
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59.发明授权Analyte detection utilizing polynucleotide sequences, composition, process and kit 失效使用多核苷酸序列，组成，过程和试剂盒的分析物检测公开(公告)号：US09134302B2
公开(公告)日：2015-09-15
申请号：US08479995
申请日：1995-06-07
申请人： Robert G. Pergolizzi , Jannis G. Stavrianopoulos , Elazar Rabbani , Dean L. Engelhardt , Stan Kline , Paula J. Olsiewski
发明人： Robert G. Pergolizzi , Jannis G. Stavrianopoulos , Elazar Rabbani , Dean L. Engelhardt , Stan Kline , Paula J. Olsiewski
CPC分类号： G01N33/53 , C12Q1/6813 , C12Q1/6876 , Y10T436/143333
摘要： A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing (B) a molecular bridging entity having thereon: (i) a portion capable of recognizing the molecularly recognizable portion on the analyte; and (ii) a portion comprising a polynucleotide sequence; and (C) a signalling entity having thereon: (i) a polynucleotide portion capable of annealing to the polynucleotide portion of the bridging entity, thereby to form a stable polynucleotide hybrid, and (ii) a signal generating portion; forming a complex comprising: (1) the analyte (A) complexed through its molecularly recognizable portion to (2) the recognizing portion of the entity (B); the entity (B) being complexed through the polynucleotide portion thereon to (3) the polynucleotide portion of the signalling entity; and detecting a signal by means of the signal generating portion present in the complex.
摘要翻译： 在样品中检测其上具有分子可识别部分的分析物（A）的方法，其包括：提供（B）其上具有其上的分子桥接实体：（i）能够识别分析物上的分子识别部分的部分; 和（ii）包含多核苷酸序列的部分; 和（C）其上具有的信令实体：（i）能够退火到所述桥接实体的多核苷酸部分从而形成稳定的多核苷酸杂交体的多核苷酸部分，和（ii）信号产生部分; 形成复合物，其包括：（1）通过其分子可识别部分复合的分析物（A）至（2）实体（B）的识别部分; 实体（B）通过其上的多核苷酸部分复合到（3）信令实体的多核苷酸部分; 以及通过存在于复合体中的信号产生部分来检测信号。
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60.发明授权Nucleic acid sequencing processes using non-radioactive detectable modified or labeled nucleotides or nucleotide analogs, and other processes for nucleic acid detection and chromosomal characterization using such non-radioactive detectable modified or labeled nucleotides or nucleotide analogs 失效使用非放射性可检测的修饰或标记核苷酸或核苷酸类似物的核酸测序方法，以及使用这种非放射性可检测修饰或标记核苷酸或核苷酸类似物的核酸检测和染色体表征的其他过程公开(公告)号：US08097405B1
公开(公告)日：2012-01-17
申请号：US08486069
申请日：1995-06-07
CPC分类号： C12Q1/6816 , A61K47/62 , C07B59/008 , C07H19/04 , C07H19/10 , C07K17/06 , C12Q1/6804 , C12Q2525/117
摘要： A process for determining the sequence of nucleic acids of interest employs nucleotides or nucleotide analogs that have been made detectable by non-radioactive modifying or labeling. Such nucleotides or nucleotide analogs are modified on the sugar moieties, the phosphate moieties or the base moieties, including base analogs. Modified nucleotide analogs can be attached to or coupled to or incorporated into DNA or RNA. The modified or labeled nucleotides or nucleotide analogs are also useful in processes for detecting the presence of nucleic acids of interest and for characterizing chromosomal sequences. Detection processes using the modified or labeled nucleotides or nucleotide analogs extend to the use of a gel for separating or resolving hybrids formed between non-radioactively labeled oligonucleotides or polynucleotides and such nucleic acids of interest.
摘要翻译： 用于确定感兴趣的核酸序列的方法使用已经通过非放射性修饰或标记可检测的核苷酸或核苷酸类似物。 这些核苷酸或核苷酸类似物在糖部分，磷酸酯部分或碱基部分（包括碱基类似物）上被修饰。 修饰的核苷酸类似物可以连接到DNA偶联或并入DNA或RNA中。 修饰或标记的核苷酸或核苷酸类似物也可用于检测感兴趣的核酸的存在和表征染色体序列的方法。 使用经修饰或标记的核苷酸或核苷酸类似物的检测方法延伸到使用凝胶来分离或拆分在非放射性标记的寡核苷酸或多核苷酸与所关注的核酸之间形成的杂交体。
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