公开(公告)号：US11110456B2

公开(公告)日：2021-09-07

申请号：US16099246

申请日：2016-07-12

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Pavel Kornilovich ,  Alexander Govyadinov ,  David P. Markel ,  Erik D. Torniainen

IPC: B01L3/00 ,  G01N30/56 ,  G01N30/10 ,  B01D15/08 ,  G01N30/40 ,  G01N30/60 ,  B81C1/00

Abstract: A microfluidic bead-packing method includes activating a first micropump to transfer active microbeads through an inlet microchannel from a bead suspension reservoir to an adsorbing channel; packing the microbeads in the adsorbing channel; and activating a second micropump to reverse flow through at least a portion of the inlet microchannel and to transfer a sample fluid through the inlet microchannel from a sample reservoir to the adsorbing channel such that the sample fluid interacts with the packed microbeads.

公开(公告)号：US20210245153A1

公开(公告)日：2021-08-12

申请号：US17049142

申请日：2018-07-09

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Pavel Kornilovich ,  Daniel Curthoys ,  Hilary Ely ,  Alexander Govyadinov

IPC: B01L3/00 ,  G01N33/487

Abstract: In one example in accordance with the present disclosure, an analyte capturing device is described. The analyte capturing device includes a first substrate having microfluidic channels disposed therein and a second substrate disposed on top of the first substrate. A chamber is disposed through the second substrate and captures beads therein, which beads adsorb analytes. The analyte capturing device includes at least one fluid ejection device disposed in the first substrate to draw an analyte-containing solution through the beads disposed within the chamber.

公开(公告)号：US11059231B2

公开(公告)日：2021-07-13

申请号：US16870132

申请日：2020-05-08

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alejandro Manuel De Pena ,  Pavel Kornilovich ,  David H. Donovan

IPC: B29C64/393 ,  B33Y30/00 ,  B29C64/40 ,  B29C64/165 ,  B29C64/386 ,  B29C64/00 ,  B33Y10/00 ,  B33Y50/02 ,  B29K105/00

Abstract: A three-dimensional object may be generated. Coalescing agent may be selectively delivered on a portion of a first layer of build material on a support member or previous layer. Energy may be applied to the first layer to cause the portion of the first layer to coalesce and solidify. A second layer of the build material may be provided on the first layer. While the second layer does not have coalescing agent delivered thereon, energy may be applied to the second layer such that energy may propagate through the second layer to the first layer to cause the portion of the first layer to coalesce and further solidify.

公开(公告)号：US10882045B2

公开(公告)日：2021-01-05

申请号：US15748975

申请日：2016-01-08

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Erik D. Torniainen ,  Alexander Govyadinov ,  Pavel Kornilovich ,  David P. Markel

IPC: B01L7/00 ,  B01L3/02 ,  B01L3/00 ,  C12M1/42

Abstract: Examples include polymerase chain reaction (PCR) devices. Example PCR devices comprise a fluid input, ejection nozzles, and a set of microfluidic channels that fluidly connect the fluid input and the ejection nozzles. Each microfluidic channel comprises a reaction chamber, and examples further comprise at least one heating element, where the at least one heating element is positioned in the reaction chamber of each microfluidic channel. The at least one heating element is to heat fluid in the reaction chamber of each fluid channel. The device may eject fluid via the ejection nozzles.

公开(公告)号：US10759085B2

公开(公告)日：2020-09-01

申请号：US15763193

申请日：2015-11-20

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Vladek Kasperchik ,  Michael G. Monroe ,  Pavel Kornilovich ,  David A. Champion

IPC: B28B1/00 ,  B33Y30/00 ,  B33Y10/00 ,  C04B35/64 ,  C04B35/63 ,  C04B35/636 ,  C04B35/638 ,  B33Y50/02 ,  B28B7/46 ,  C04B35/634 ,  B29C64/165 ,  B33Y70/00

Abstract: In a three-dimensional printing method example, build material granules are applied. Each of the build material granules includes uncoated, primary ceramic particles agglomerated together by a binder that is soluble in a primary solvent of a fusing agent. The fusing agent is selectively applied on at least a portion of the build material granules. The binder dissolves and a green body including a slurry of the uncoated, ceramic particles is created.

公开(公告)号：US10696939B2

公开(公告)日：2020-06-30

申请号：US16079482

申请日：2016-04-22

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alexander Govyadinov ,  Erik D. Torniainen ,  David P. Markel ,  Pavel Kornilovich

IPC: B01L3/00 ,  C12M1/00 ,  C12N1/06

Abstract: In an example implementation, a method of cell lysis includes moving cell fluid from a first reservoir through a microfluidic channel toward a second reservoir, activating a lysing element multiple times as a cell from the cell fluid passes through the microfluidic channel, and moving lysate fluid that results from the activating through the microfluidic channel and into the second reservoir.

公开(公告)号：US20200147605A1

公开(公告)日：2020-05-14

申请号：US16616947

申请日：2017-07-18

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Jeremy Harlan Donaldson ,  Stephen Lee Frey ,  Pavel Kornilovich ,  Alexander Govyadinov

IPC: B01L3/00

Abstract: A sample test device is provided that includes a body having an insertion surface spaced apart from a distal end portion and a fluid manipulating assembly disposed in the distal end portion. A mixing receptacle is defined in the fluid manipulating assembly and provides a volume to mix a test mixture. A plunger is disposed in the body and creates a positive air pressure in the mixing receptacle when inserted into the body. A test die is disposed in the fluid manipulation assembly and a fluid path extends from the mixing receptacle to the test die. Activation of the plunger creates a positive pressure in the mixing receptacle to force the test mixture to flow from the mixing receptacle to the test die.

公开(公告)号：US20190291102A1

公开(公告)日：2019-09-26

申请号：US16317429

申请日：2016-09-23

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Pavel Kornilovich ,  Alexander N. GOVYADINOV

IPC: B01L3/00

Abstract: A microfluidic device may include at least four interconnected microfluidic channels and a set of fluid actuators. The set of fluid actuators may include a fluid actuator asymmetrically located within at least two of the at least four interconnected microfluidic channels. Each of the at least four interconnected microfluidic channels may be activated to a fluid inputting state, a fluid outputting state and a fluid blocking state in response to selective actuation of different combinations of fluid actuators of the set.

公开(公告)号：US20180230518A1

公开(公告)日：2018-08-16

申请号：US15955364

申请日：2018-04-17

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Pavel Kornilovich ,  Erik D. Tornianinen ,  Alexander Govyadinov ,  David P. Markel

IPC: C12Q1/686

CPC classification number: C12Q1/686 ,  B01L3/50273 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/1827

Abstract: In one embodiment, a polymerase chain reaction (PCR) system includes a mixture chamber, a denature chamber, an annealing chamber, an extension chamber, and a product chamber, that are fluidically coupled to one another through a plurality of microfluidic channels. An inertial pump is associated with each microfluidic channel, and each inertial pump includes a fluid actuator integrated asymmetrically within its associated microfluidic channel. The fluid actuators are capable of selective activation to circulate fluid between the chambers in a controlled cycle.

公开(公告)号：US20180214866A1

公开(公告)日：2018-08-02

申请号：US15748456

申请日：2016-01-08

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Erik D Torniainen ,  Alexander Govyadinov ,  Pavel Kornilovich ,  David P Markel

IPC: B01L3/00 ,  C12M3/06 ,  C12M1/34

CPC classification number: B01L3/50273 ,  B01L3/502784 ,  B01L7/52 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0896 ,  B01L2300/1827 ,  B01L2400/0442 ,  B01L2400/0481 ,  C12M23/16 ,  C12M41/12 ,  C12Q1/686

Abstract: Examples include polymerase chain reaction (PCR) devices. Example PCR devices comprise a fluid input, a fluid output, and a set of microfluidic channels that fluidly connect the fluid input and the fluid output. Each microfluidic channel comprises a reaction chamber, and examples further comprise at least one heating element, where the at least one heating element is positioned in the reaction chamber of each microfluidic channel. The at least one heating element is to heat fluid in the reaction chamber of each fluid channel, and the at least one heating element is to pump fluid to the reaction chamber and from the reaction chamber of each microfluidic channel.