公开(公告)号：US08030461B2

公开(公告)日：2011-10-04

申请号：US10510971

申请日：2003-04-15

申请人： Tetsuo Kojima

发明人： Tetsuo Kojima

IPC分类号： C07H21/04

CPC分类号： C07K16/468 ,  C12N15/1093

摘要： Bispecific diabody libraries can be constructed from scFv libraries efficiently and without troublesome procedures by a single treatment, placing restriction enzyme sites appropriately for antigen-encoding regions.

摘要翻译： 双特异性双抗体文库可以通过单次处理有效地由scFv文库构建而不需要麻烦的程序，为抗原编码区域适当地设置限制酶位点。

公开(公告)号：US07948673B2

公开(公告)日：2011-05-24

申请号：US12233174

申请日：2008-09-18

申请人： Masashi Yoshimura ,  Takatomo Sasaki ,  Yusuke Mori ,  Muneyuki Nishioka ,  Tomotaka Katsura ,  Tetsuo Kojima ,  Junichi Nishimae

发明人： Masashi Yoshimura ,  Takatomo Sasaki ,  Yusuke Mori ,  Muneyuki Nishioka ,  Tomotaka Katsura ,  Tetsuo Kojima ,  Junichi Nishimae

IPC分类号： G02F1/35 ,  H01S3/10

CPC分类号： G02F1/37 ,  G02F1/3551 ,  G02F2001/3505 ,  G02F2001/354

摘要： An optical wavelength conversion element includes a cesium-lithium-borate crystal processed into a 10-mm long optical element cut in an orientation that allows a fourth harmonic of a Nd:YAG laser to be generated. A transmittance (Ta) at 3589 cm−1 in an infrared transmission spectrum of the optical element is used as an index that indicates a content of water impurities in the crystal and is independent of a polarization direction. An actual measurement of the transmittance Ta is at least 1%, without taking into account loss at an optically polished surface of the crystal. A wavelength conversion device, a ultraviolet laser irradiation apparatus, a laser processing system, and a method of manufacturing an optical wavelength conversion element are also described.

摘要翻译： 光波长转换元件包括加工成允许产生Nd：YAG激光器的四次谐波的取向切割的10mm长的光学元件的铯 - 硼酸锂晶体。 光学元件的红外透射光谱中的3589cm -1处的透射率（Ta）被用作指示晶体中的水杂质含量并且与偏振方向无关的指标。 透射率Ta的实际测量值至少为1％，而不考虑在晶体的光学抛光表面处的损耗。 还描述了波长转换装置，紫外激光照射装置，激光加工系统和制造光波长转换元件的方法。

公开(公告)号：US07286282B2

公开(公告)日：2007-10-23

申请号：US10853223

申请日：2004-05-26

申请人： Tetsuo Kojima ,  Susumu Konno ,  Junichi Nishimae ,  Shinsuke Yura ,  Kazutoshi Morikawa ,  Atsuhiro Sono ,  Yukio Sato

发明人： Tetsuo Kojima ,  Susumu Konno ,  Junichi Nishimae ,  Shinsuke Yura ,  Kazutoshi Morikawa ,  Atsuhiro Sono ,  Yukio Sato

IPC分类号： G02F1/35 ,  H01S3/10

CPC分类号： G02F1/353

摘要： A fundamental laser beam is wavelength converted through nonlinear optical crystals by traveling in one direction, sequentially through two nonlinear optical crystals arranged in series. A wavelength-converted laser beam is generated and includes wavelength-converted laser beams having polarized directions differing from each other by angles in a range from 45° to 90°. The two nonlinear optical crystals have crystal orientation axes differing by 45° to 90° when viewed along the optical axis of the laser beam.

摘要翻译： 通过沿一个方向行进，通过非线性光学晶体波长转换基本激光束，依次通过串联布置的两个非线性光学晶体。 产生波长转换的激光束，并且包括波长转换的激光束，其具有彼此相差45°至90°的角度的偏振方向。 当沿着激光束的光轴观察时，两个非线性光学晶体具有45°至90°的晶体取向轴。

公开(公告)号：US20070087381A1

公开(公告)日：2007-04-19

申请号：US10510971

申请日：2003-04-15

申请人： Tetsuo Kojima

发明人： Tetsuo Kojima

IPC分类号： C40B40/10 ,  C40B50/06 ,  C07K16/18 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06

CPC分类号： C07K16/468 ,  C12N15/1093

摘要： Bispecific diabody libraries can be constructed from scFv libraries efficiently and without troublesome procedures by a single treatment, placing restriction enzyme sites appropriately for antigen-encoding regions.

摘要翻译： 双特异性双抗体文库可以通过单次处理有效地由scFv文库构建而不需要麻烦的程序，为抗原编码区域适当地设置限制酶位点。

公开(公告)号：US20060269989A1

公开(公告)日：2006-11-30

申请号：US10560098

申请日：2004-06-11

申请人： Taro Miyazaki ,  Tetsuo Kojima

发明人： Taro Miyazaki ,  Tetsuo Kojima

IPC分类号： C12P21/06 ,  C07H21/04 ,  C07K16/18 ,  C12N5/06

CPC分类号： C07K16/468 ,  C07K16/00 ,  C07K16/2866 ,  C07K2317/626 ,  C12N2510/02

摘要： Focusing on the fact that antibody molecules with one H chain are not secreted when using the “knobs-into-holes” method, the present inventors revealed that desired bispecific antibodies can be preferentially formed by first expressing the H and L chains of one arm, and then suppressing their expression, followed by expressing the H and L chains of the other arm so that first desired HL molecules (HaLa and HbLb) are constructed, and then the H chains are paired with each other (H2L2). The present invention was thus completed.

摘要翻译： 关注当使用“旋钮孔”方法时，不分泌具有一个H链的抗体分子的事实，本发明人揭示了可以通过首先表达一个臂的H和L链来优先形成所需的双特异性抗体， 然后抑制其表达，随后表达另一臂的H和L链，使得构建第一所需的HL分子（HaLa和HbLb），然后H链彼此配对（H 2' SUB＆gt; 2＆lt; 2＆gt;）。 从而完成了本发明。

公开(公告)号：US20060204496A1

公开(公告)日：2006-09-14

申请号：US10540643

申请日：2003-11-28

申请人： Tetsuo Kojima ,  Chiaki Senoo ,  Osamu Natori ,  Keiko Kasutani ,  Shiny Ishi

发明人： Tetsuo Kojima ,  Chiaki Senoo ,  Osamu Natori ,  Keiko Kasutani ,  Shiny Ishi

IPC分类号： A61K39/395 ,  C07K16/28

CPC分类号： C07K16/2866 ,  C07K2317/31 ,  C07K2317/622

摘要： Animals were immunized with either the A or B chain of a receptor, and then mRNA was extracted from the spleen cells of the animals, and the variable regions of the L and H chains were isolated by RT-PCR using primers for variable regions comprising CDRs. Single-chain Fv was synthesized by assembly PCR to construct a phage library. Clones for antigen-bound antibodies were concentrated and cloned by panning. An expression vector for scFv-CH1-Fc was prepared by inserting a single-chain variable region between CH1-hinge-CH2-CH3 and the signal sequence for animal cells. Various combinations of such expression vectors were introduced into cells to express antibodies, and antibody clones exhibiting ligand-like activity were selected.

摘要翻译： 用受体的A或B链免疫动物，然后从动物的脾细胞中提取mRNA，并且通过RT-PCR分离L和H链的可变区，使用包含CDR的可变区的引物 。 通过装配PCR合成单链Fv构建噬菌体文库。 通过淘选浓缩抗原结合抗体的克隆并克隆。 通过在CH1铰链-CH2-CH3和动物细胞的信号序列之间插入单链可变区来制备scFv-CH1-Fc的表达载体。 将这些表达载体的各种组合引入细胞中以表达抗体，并选择显示配体样活性的抗体克隆。

公开(公告)号：US20050225837A1

公开(公告)日：2005-10-13

申请号：US10511170

申请日：2001-12-12

申请人： Tetsuo Kojima ,  Susumu Konno ,  Shuichi Fujikawa ,  Koji Yasui ,  Takatomo Sasaki ,  Yusuke Mori ,  Masashi Yoshimura

发明人： Tetsuo Kojima ,  Susumu Konno ,  Shuichi Fujikawa ,  Koji Yasui ,  Takatomo Sasaki ,  Yusuke Mori ,  Masashi Yoshimura

IPC分类号： G02F1/35 ,  G02F2/02

CPC分类号： G02F1/3501 ,  G02F2001/3505

摘要： In a wavelength converting method, an ambient that is in contact with a surface of a non-linear optical crystal from which wavelength-converted light is outputted is a gas that is lower in content of nitrogen than air. A wavelength converting device includes a device for controlling the ambient in contact with a surface of the non-linear optical crystal from which the wavelength-converted light is outputted so the ambient is lower in nitrogen than air. A laser machining device includes the wavelength converting device.

摘要翻译： 在波长转换方法中，与输出波长转换光的非线性光学晶体的表面接触的环境是氮含量低于空气的气体。 波长转换装置包括用于控制与非线性光学晶体的表面接触的环境的装置，从该波长转换光输出波长转换的光，使得氮气中的环境比空气低。 激光加工装置包括波长转换装置。

公开(公告)号：US5889808A

公开(公告)日：1999-03-30

申请号：US825048

申请日：1997-03-27

申请人： Tetsuo Kojima ,  Koji Yasui

发明人： Tetsuo Kojima ,  Koji Yasui

IPC分类号： H01S3/042 ,  H01S3/04 ,  H01S3/094 ,  H01S3/0941

CPC分类号： H01S3/0941 ,  H01S3/0407 ,  H01S3/042 ,  H01S3/094057

摘要： To inexpensively and in a simple configuration provide a solid laser amplifier and a solid laser unit capable of generating a high output and high quality laser beam, the unit includes a solid laser medium 5A containing active solid media; a flow tube 14 or the like allowing a cooling medium 24 for cooling the solid laser medium 5A; and a laser array 12 so controlled in temperature as to emit an excitation light 9 for exciting the solid laser medium 5A, the wavelength of which lies within the absorption spectra of a solid laser medium 5A yet does not coincide with that of the absorption spectrum peak of a solid laser medium 5A.

摘要翻译： 为了廉价并且以简单的配置提供了能够产生高输出和高质量激光束的固体激光放大器和固体激光器单元，该单元包括含有活性固体介质的固体激光介质5A; 流动管14等，用于冷却固体激光介质5A的冷却介质24; 以及如此控制的激光阵列12，以发射用于激发固体激光介质5A的激发光9，其激光位于固体激光介质5A的吸收光谱内，但与吸收光谱峰的波长不一致 的固体激光介质5A。

公开(公告)号：US5351259A

公开(公告)日：1994-09-27

申请号：US965716

申请日：1992-10-23

申请人： Akira Ishimori ,  Takashi Yamamoto ,  Tetsuo Kojima

发明人： Akira Ishimori ,  Takashi Yamamoto ,  Tetsuo Kojima

IPC分类号： H01S3/06 ,  H01S3/08 ,  H01S3/0941 ,  H01S5/40 ,  H01S3/07 ,  H01S3/094 ,  H01S3/16

CPC分类号： H01S3/09415 ,  H01S3/0606 ,  H01S3/08072 ,  H01S3/0941 ,  H01S3/2383 ,  H01S5/4025

摘要： A semiconductor-laser-pumped solid-state laser apparatus having a semiconductor laser device, a solid state laser medium, and laser resonators. The semiconductor laser device has a plurality of emission points which are arranged on a straight line and from which pump light is generated. The laser resonators are disposed in correspondence with the emission points. A plurality of solid state laser beams can be generated by using one solid state laser medium.

摘要翻译： 具有半导体激光器件，固态激光介质和激光谐振器的半导体激光泵浦固态激光器件。 半导体激光装置具有多个发射点，其布置在直线上并从其产生泵浦光。 激光谐振器与发射点对应地设置。 通过使用一个固态激光介质可以产生多个固态激光束。