公开(公告)号：US20050013804A1

公开(公告)日：2005-01-20

申请号：US10380049

申请日：2001-09-12

申请人： Yukio Kato ,  Shinichi Tsutsumi ,  Atsushi Shimazu

发明人： Yukio Kato ,  Shinichi Tsutsumi ,  Atsushi Shimazu

IPC分类号： C12N5/02 ,  C12N5/0775 ,  A61K45/00 ,  C12N5/08

CPC分类号： C12N5/0663 ,  C12N2501/115

摘要： This invention provides a novel method of culturing mesenchymal stem cells whereby a remarkably larger number of mesenchymal stem cells can be obtained compared with the conventional culture methods. This culture method is characterized by adding to a medium a fibroblast growth factor (FGF) as a substance which stimulates the proliferation potency of the mesenchymal stem cells while retaining the pluripotency thereof and prolongs the life. According to the method, mesenchymal stem cells can be cultured at least over 30 generations and, moreover, about 105 to 106 times as much as cell count in the conventional culture methods can be obtained.

摘要翻译： 本发明提供了培养间充质干细胞的新方法，与常规培养方法相比，可以获得显着更多数量的间充质干细胞。 该培养方法的特征在于将培养成纤维细胞生长因子（FGF）作为刺激间充质干细胞增殖能力的物质，同时保留其多能性并延长其寿命的物质。 根据该方法，可以培养至少30代以上的间充质干细胞，此外，可以获得常规培养方法中细胞计数的约10 5〜10 6倍。

公开(公告)号：US5670338A

公开(公告)日：1997-09-23

申请号：US455550

申请日：1995-05-31

申请人： Kazuo Murakami ,  Naoto Ueno ,  Yukio Kato

发明人： Kazuo Murakami ,  Naoto Ueno ,  Yukio Kato

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P3/02 ,  A61P19/10 ,  A61P43/00 ,  C07K14/00 ,  C07K14/51 ,  C12N1/21 ,  C12N15/12 ,  C12N15/16 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91 ,  C12N15/00

CPC分类号： C07K14/51 ,  A61K38/00

摘要： Disclosed are (1) a Xenopus laevis bone morphogenetic protein (BMP), (2) a DNA comprising a DNA segment coding for a Xenopus laevis BMP, (3) a transformant bearing a DNA comprising a DNA segment coding for a Xenopus laevis BMP and (4) a method for preparing the Xenopus laevis BMP which comprises culturing the described in (3), producing and accumulating the protein in a culture, and collecting the protein thus obtained. Cells transinfected or transformed with the DNA allow large amounts of the Xenopus laevis BMP mature peptides to be produced, which causes the advantageous production of the peptides, which promote the synthesis of proteoglycan and can also be utilized for analysis of the mechanism of organism, particularly human bone-cartilage morphogenetic reaction, and as therapeutic agents for osteoporosis.

摘要翻译： 公开了（1）非洲爪蟾骨形态发生蛋白（BMP），（2）包含编码非洲爪蟾BMP的DNA区段的DNA，（3）携带DNA的转化体，其包含编码非洲爪蟾BMP的DNA区段和 （4）一种制备非洲爪蟾BMP的方法，其包括培养（3）中所述的培养物，在培养物中产生和积累蛋白质，并收集由此获得的蛋白质。 用DNA转染或转化的细胞允许产生大量非洲爪蟾成熟肽，这导致肽的有利产生，促进蛋白多糖的合成，并且还可用于分析生物体的机制，特别是 人骨软骨形态发生反应，以及作为骨质疏松症的治疗剂。

公开(公告)号：US5467648A

公开(公告)日：1995-11-21

申请号：US120542

申请日：1993-09-14

申请人： Shinya Igarashi ,  Hiroshi Hirayama ,  Chihiro Kobayashi ,  Mitsukuni Tsutsui ,  Yukio Kato

发明人： Shinya Igarashi ,  Hiroshi Hirayama ,  Chihiro Kobayashi ,  Mitsukuni Tsutsui ,  Yukio Kato

IPC分类号： F02D45/00 ,  G01F1/68 ,  G01F1/684 ,  G01F5/00

CPC分类号： G01F5/00 ,  G01F1/6842

摘要： An air flow rate measuring device for measuring intake air flow rate in an automotive engine, improved to reduce any error attributable to variation in the configurations of air cleaner and/or duct forming air passage upstream of the flow rate measuring device, thus offering high accuracy of the flow rate measurement. In one aspect of the invention, the inlet opening of a sub-air passage is disposed to contain the axis of a main air passage, while the outlet opening of the sub-air passage is disposed in the vicinity of a wall defining the main air passage. In order to cause a local reduction of the pressure of the principal of the air flow at the outlet opening, a protrusion or a flat surface which causes impediment to the flow if air in the main air passage is provided immediately upstream of the outlet opening. In another aspect, the inlet surface in which the inlet of the sub-air passage opens is stepped in the direction of flow of the air so as to enable air to be introduced into air flow rate measuring portion in the sub-air passage from different regions in the main air passage while giving a preselected order of preference to these different regions.

摘要翻译： 一种用于测量汽车发动机中的进气流量的空气流量测量装置，其改进以减少由于流量测量装置上游空气滤清器和/或导管形成空气通道的结构变化引起的任何误差，从而提供高精度 的流量测量。 在本发明的一个方面，副空气通道的入口设置成容纳主空气通道的轴线，而副空气通道的出口设置在限定主空气的壁的附近 通道。 为了使出口开口处的空气流的本征压力局部地减小，突起或平坦表面在紧邻导出口的上游处设置有主空气通道中的空气，从而阻碍流动。 另一方面，副空气通路的入口打开的入口面沿着空气的流动方向阶梯状，以使得空气能够从不同的空气通道引入到副空气通道中的空气流量测量部分 主空气通道中的区域，同时给予这些不同区域的预选顺序。

公开(公告)号：US5453419A

公开(公告)日：1995-09-26

申请号：US56564

申请日：1993-04-30

申请人： Kazuo Murakami ,  Naoto Ueno ,  Yukio Kato

发明人： Kazuo Murakami ,  Naoto Ueno ,  Yukio Kato

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P3/02 ,  A61P19/10 ,  A61P43/00 ,  C07K14/00 ,  C07K14/51 ,  C12N1/21 ,  C12N15/12 ,  C12N15/16 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91 ,  A61K38/17 ,  C07K14/435

CPC分类号： C07K14/51 ,  A61K38/00

摘要： Disclosed are (1) a Xenopus laevis bone morphogenetic protein (BMP), (2) a DNA comprising a DNA segment coding for a Xenopus laevis BMP, (3) a transformant bearing a DNA comprising a DNA segment coding for a Xenopus laevis BMP and (4) a method for preparing the Xenopus laevis BMP which comprises culturing the described in (3), producing and accumulating the protein in a culture, and collecting the protein thus obtained. Cells transfected or transformed with the DNA allow large amounts of the Xenopus laevis BMP mature peptides to be produced, which causes the advantageous production of the peptides, which promote the synthesis of proteoglycan and can also be utilized for analysis of the mechanism of organism, particularly human bone-cartilage morphogenetic reaction, and as therapeutic agents for osteoporosis.

摘要翻译： 公开了（1）非洲爪蟾骨形态发生蛋白（BMP），（2）包含编码非洲爪蟾BMP的DNA区段的DNA，（3）携带DNA的转化体，其包含编码非洲爪蟾BMP的DNA区段和 （4）一种制备非洲爪蟾BMP的方法，其包括培养（3）中所述的培养物，在培养物中产生和积累蛋白质，并收集由此获得的蛋白质。 用DNA转染或转化的细胞允许产生大量的非洲爪蟾成熟肽，这导致肽的有利生产，其促进蛋白多糖的合成，并且还可以用于分析生物体的机制，特别是 人骨软骨形态发生反应，以及作为骨质疏松症的治疗剂。

公开(公告)号：US4247748A

公开(公告)日：1981-01-27

申请号：US954643

申请日：1978-10-25

申请人： Kunihiko Sato ,  Tetsuro Urata ,  Kobun Asakawa ,  Haruo Taguchi ,  Yukio Kato ,  Takeo Uebayashi

发明人： Kunihiko Sato ,  Tetsuro Urata ,  Kobun Asakawa ,  Haruo Taguchi ,  Yukio Kato ,  Takeo Uebayashi

IPC分类号： B21B27/00 ,  B23H9/04

CPC分类号： B23H9/04

摘要： There is disclosed a method for giving by electrical discharge machining a uniform satin finish to the roll surface of a work in roll form in a short period of time. To prevent the occurrence of any streaky pattern on the satin finish work surface, a plurality of divided electrodes, which are divided and arranged at predetermined spaces, are each fed traversely a distance corresponding to the electrode mounting pitch while maintaining the predetermined spacing between the electrodes. To improve the working efficiency, a plurality of rows of divided electrodes are provided, and by virtue of this improved working efficiency, the required satinizing electrical discharge machining of two or four rolls for use on a rolling mill can be completed within the life time of the satinized rolls being used on the rolling mill.

摘要翻译： 公开了一种在短时间内通过放电加工均匀的缎面精加工到卷筒形式的工件的辊表面的方法。 为了防止在缎面加工表面上出现任何条纹图案，以预定间隔分割和布置的多个分割电极分别被馈送到与电极安装间距相对应的距离，同时保持电极之间的预定间隔 。 为了提高工作效率，提供了多排分隔电极，并且由于这种提高的工作效率，在轧机上使用的两辊或四辊所需的缎光放电加工可在 在轧机上使用缎纹辊。

公开(公告)号：US4067809A

公开(公告)日：1978-01-10

申请号：US681041

申请日：1976-04-28

申请人： Yukio Kato

发明人： Yukio Kato

IPC分类号： A01K63/04 ,  E04H3/20

CPC分类号： A01K63/045

摘要： A filtering system is disclosed for effectively filtering the water of aquariums for tropical or salt-water fish, the water of fish-farming ponds, and/or any other type of fish-farming water. The system includes a filtration tank partitioned into a storage chamber, a filtration chamber, and a discharge chamber, and the fish-farming water is successively passed through the storage and filtration chambers so as to be purified, and is discharged along the water surface by means of a discharge mechanism within the discharge chamber. A lift pipe is provided at one end of the storage chamber, and filters and vacancies are alternately arranged in a parallel manner within the filtration chamber, with an open spaced defined thereabove. The water level of the filtration tank is maintained lower than the top of the filters, and the filter layers may be sets of two water-passing plates, such as, for example, multipore plates, net-type plates, or the like, with the filter medium, such as, for example, glass wool, sand, or the like, packed therebetween or attached thereto. The discharge chamber is equipped with a discharge pipe which has an opening directed along the surface of the water, and in this manner, the fish-farming water is prepurified in the storage chamber, further purified in the filtration chamber, and discharged along the water surface so as to be uniformly circulated in an installation for fish-farming. The water is constantly maintained in an aerobic state and an extremely high purification efficiency is obtained.

摘要翻译： 公开了一种过滤系统，用于有效过滤热带或咸水鱼，养鱼池塘的水和/或任何其他类型的养殖水的水族箱的水。 该系统包括分隔成储存室，过滤室和排出室的过滤罐，并且将养殖水依次通过储存和过滤室，以便净化，并且沿着水面通过 在放电室内的放电机构的装置。 提升管设置在储存室的一端，并且过滤器和空位在过滤室内以平行的方式交替布置，并且在其上方具有开口间隔。 过滤罐的水位保持低于过滤器的顶部，过滤层可以是两个水通过板的组，例如多孔板，网状板等，其中 过滤介质，例如玻璃棉，沙子等，在其间填充或附着于其上。 排气室配有排水管，排水管具有沿水面的开口，以这种方式，在储存室中预先烹制鱼养水，在过滤室中进一步净化，并沿着水排出 以便在用于养鱼的装置中均匀地循环。 水经常保持在好氧状态，并且获得极高的净化效率。

公开(公告)号：US08349935B2

公开(公告)日：2013-01-08

申请号：US10551872

申请日：2004-04-02

申请人： Akihiro Ohashi ,  Kazuya Tanaka ,  Yukio Kato ,  Jun Takagi ,  Shinichiro Yamada ,  Hiroyuki Mori ,  Yuko Fujihira

发明人： Akihiro Ohashi ,  Kazuya Tanaka ,  Yukio Kato ,  Jun Takagi ,  Shinichiro Yamada ,  Hiroyuki Mori ,  Yuko Fujihira

IPC分类号： C08K3/22

CPC分类号： C08K3/22 ,  C08K3/013 ,  C08K9/00 ,  C08L67/02 ,  C08L67/04 ,  Y10T428/2991 ,  Y10T428/2998 ,  C08L2666/18

摘要： To obtain injection molded objects having biodegradability and excellent flame retardance and mechanical strength, the injection molded object includes a lactic acid based resin; and a metal hydroxide in a ratio of 5 to 40 mass parts per 100 mass parts of the lactic acid based resin. The metal hydroxide is surface-treated, and Na2O (w-Na2O) present on a surface of grains of the metal hydroxide is 0.1 mass % or less based on the total mass of the metal hydroxide. Preferably, the injection molded object contains an aliphatic polyester other than the lactic acid based resin or an aromatic-aliphatic polyester in a ratio of 20 to 80 mass parts per 100 mass parts of the lactic acid based resin.

摘要翻译： 为了获得具有生物降解性和优异的阻燃性和机械强度的注射成型体，注射成型物体包括乳酸类树脂; 和相对于100质量份乳酸类树脂为5〜40质量份的金属氢氧化物。 对金属氢氧化物进行表面处理，以金属氢氧化物的总质量计，存在于金属氢氧化物粒子表面的Na 2 O（w-Na 2 O）为0.1质量％以下。 优选地，注射成型体中含有乳酸类树脂以外的脂肪族聚酯或芳族 - 脂肪族聚酯，相对于100质量份乳酸类树脂为20〜80质量份。

公开(公告)号：US20120329087A1

公开(公告)日：2012-12-27

申请号：US13583150

申请日：2011-03-10

申请人： Toshie Tsuchiya ,  Koichiro Tsuji ,  Yukio Kato ,  Jin Chang Shao ,  Maiko Hara

发明人： Toshie Tsuchiya ,  Koichiro Tsuji ,  Yukio Kato ,  Jin Chang Shao ,  Maiko Hara

IPC分类号： C12N5/0775 ,  C12Q1/06

CPC分类号： C12N5/0663 ,  A61K35/12 ,  C12N5/0668 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15

摘要： A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium “A” containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A).

摘要翻译： 通过无血清或低血清培养物产生含有维持免疫抑制能力的间充质干细胞的细胞制剂。 一种含有间充质干细胞的细胞制剂的制备方法，包括以下步骤：（A）在含有FGF，PDGF，TGF-β1，HGF，EGF的无血清培养基A中增殖间充质干细胞， 至少一种磷脂和至少一种脂肪酸; 和（B）从步骤（A）中因此增殖的间充质干细胞筛选其免疫抑制能力维持或改善的间充质干细胞。

公开(公告)号：US08116176B2

公开(公告)日：2012-02-14

申请号：US12950146

申请日：2010-11-19

申请人： Yukio Kato ,  Irizo Naniwa

发明人： Yukio Kato ,  Irizo Naniwa

IPC分类号： G11B11/00

CPC分类号： G11B5/314 ,  G11B5/1278 ,  G11B5/6088 ,  G11B2005/0005 ,  G11B2005/0021

摘要： A thermally assisted magnetic head-slider includes an air-bearing slider, a metal film, and a semiconductor laser unit. The metal film having an aperture in a part through which light from the semiconductor laser unit passes is disposed between a surface opposite to an air-bearing surface of the air-bearing slider and the semiconductor laser unit, and a material to adjust refractive index is provided in the aperture. A bottom surface of the metal film including the material to adjust refractive index is disposed to be in close contact with a surface opposite to the air-bearing surface side of the air-bearing slider, and the semiconductor laser unit is disposed to be in close contact with a top surface of the metal film including the material to adjust refractive index.

摘要翻译： 热辅助磁头滑块包括空气轴承滑块，金属薄膜和半导体激光单元。 在半导体激光单元的光通过的部分中具有孔的金属膜设置在与空气轴承滑块的空气轴承表面相对的表面和半导体激光单元之间，并且调节折射率的材料是 提供在孔中。 包括调整折射率的材料的金属膜的底面设置成与空气轴承滑块的与空气轴承表面侧相对的表面紧密接触，并且半导体激光单元被设置为接近 与包括该材料的金属膜的顶表面接触以调节折射率。

公开(公告)号：US20110242954A1

公开(公告)日：2011-10-06

申请号：US13078580

申请日：2011-04-01

申请人： MASARU FURUKAWA ,  Yukio Kato ,  Junguo Xu

发明人： MASARU FURUKAWA ,  Yukio Kato ,  Junguo Xu

IPC分类号： G11B27/36

CPC分类号： G11B5/6076 ,  G11B5/455

摘要： A magnetic head slider inspection device including a motor unit for revolving a disk medium, a slider unit provided with a reproducing head, which is levitated from a surface of the disk medium under force generated by the revolving disk medium, a magnetic field generator positioned at an opposite side of the disk medium with respect to the slider unit for generating a magnetic field substantially perpendicular to the disk medium surface, and a circuit unit for generating the magnetic field from the magnetic field generator, and measuring a resistance value of the reproducing head levitated from the disk medium surface.

摘要翻译： 一种磁头滑动器检查装置，包括用于旋转盘介质的马达单元，设置有再现头的滑块单元，其由旋转盘介质产生的力从盘介质的表面浮动;磁场发生器，位于 磁盘介质的相对于用于产生基本上垂直于盘介质表面的磁场的滑块单元的相对侧;以及电路单元，用于从磁场发生器产生磁场，并测量再现头的电阻值 从磁盘介质表面浮起。