公开(公告)号：US20120329087A1

公开(公告)日：2012-12-27

申请号：US13583150

申请日：2011-03-10

申请人： Toshie Tsuchiya ,  Koichiro Tsuji ,  Yukio Kato ,  Jin Chang Shao ,  Maiko Hara

发明人： Toshie Tsuchiya ,  Koichiro Tsuji ,  Yukio Kato ,  Jin Chang Shao ,  Maiko Hara

IPC分类号： C12N5/0775 ,  C12Q1/06

CPC分类号： C12N5/0663 ,  A61K35/12 ,  C12N5/0668 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15

摘要： A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium “A” containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A).

摘要翻译： 通过无血清或低血清培养物产生含有维持免疫抑制能力的间充质干细胞的细胞制剂。 一种含有间充质干细胞的细胞制剂的制备方法，包括以下步骤：（A）在含有FGF，PDGF，TGF-β1，HGF，EGF的无血清培养基A中增殖间充质干细胞， 至少一种磷脂和至少一种脂肪酸; 和（B）从步骤（A）中因此增殖的间充质干细胞筛选其免疫抑制能力维持或改善的间充质干细胞。

公开(公告)号：US09394521B2

公开(公告)日：2016-07-19

申请号：US13583150

申请日：2011-03-10

申请人： Toshie Tsuchiya ,  Koichiro Tsuji ,  Yukio Kato ,  Jin Chang Shao ,  Maiko Hara

发明人： Toshie Tsuchiya ,  Koichiro Tsuji ,  Yukio Kato ,  Jin Chang Shao ,  Maiko Hara

IPC分类号： C12N5/0775 ,  C12Q1/06 ,  A61K35/12

CPC分类号： C12N5/0663 ,  A61K35/12 ,  C12N5/0668 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15

摘要： A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium “A” containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A).

摘要翻译： 通过无血清或低血清培养物产生含有维持免疫抑制能力的间充质干细胞的细胞制剂。 一种含有间充质干细胞的细胞制剂的制备方法，包括以下步骤：（A）在含有FGF，PDGF，TGF-βb，HGF，HGF的无血清培养基“A”中增殖间充质干细胞 EGF，至少一种磷脂和至少一种脂肪酸; 和（B）从步骤（A）中因此增殖的间充质干细胞筛选其免疫抑制能力维持或改善的间充质干细胞。

公开(公告)号：US20110212523A1

公开(公告)日：2011-09-01

申请号：US13127774

申请日：2009-10-22

申请人： Yukio Kato ,  Jin Chang Shao ,  Koichiro Tsuji

发明人： Yukio Kato ,  Jin Chang Shao ,  Koichiro Tsuji

IPC分类号： C12N5/077

CPC分类号： C12N5/0654 ,  C12N2500/25 ,  C12N2500/42 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/39 ,  C12N2501/40 ,  C12N2506/1353

摘要： Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and β-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid.

摘要翻译： 本发明提供一种分化诱导培养基添加剂，其用于诱导选自干细胞，牙髓细胞，牙周韧带细胞，胎盘，羊膜和成纤维细胞中的至少一种类型的细胞的骨分化。 无血清条件，以及使用分化诱导培养基添加剂。 用于在无血清条件下诱导干细胞分化的本发明的分化诱导培养基添加剂至少含有至少一种选自EGF，FGF和PDGF的生长因子; 地塞米松 和甘油磷酸酯。 本发明的分化诱导培养基添加剂不需要抗坏血酸2-磷酸酯和ITS，它们通常对骨分化至关重要。 此外，通过添加磷脂可以促进骨分化。

公开(公告)号：US20100279412A1

公开(公告)日：2010-11-04

申请号：US12160481

申请日：2007-01-11

申请人： Yukio Kato ,  Jin Chang Shao ,  Yuki Katsura ,  Koichiro Tsuji

发明人： Yukio Kato ,  Jin Chang Shao ,  Yuki Katsura ,  Koichiro Tsuji

IPC分类号： C12N5/02

CPC分类号： C12N5/0663 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0686 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/10 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/40

摘要： Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.

摘要翻译： 公开了：含有特定生长因子和至少一种磷脂的培养基; 用于制备培养基的组合物; 一套工具 和一种方法。 可以提供一种技术，其使用无血清或低血清培养基，并且与在含血清培养基中培养获得的促进作用相当，对动物细胞的增殖具有促进作用。

公开(公告)号：US09074176B2

公开(公告)日：2015-07-07

申请号：US12160481

申请日：2007-01-11

申请人： Yukio Kato ,  Jin Chang Shao ,  Yuki Katsura ,  Koichiro Tsuji

发明人： Yukio Kato ,  Jin Chang Shao ,  Yuki Katsura ,  Koichiro Tsuji

IPC分类号： C12N5/02 ,  C12N5/00

CPC分类号： C12N5/0663 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0686 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/10 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/40

摘要： Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.

摘要翻译： 公开了：含有特定生长因子和至少一种磷脂的培养基; 用于制备培养基的组合物; 一套工具 和一种方法。 可以提供一种技术，其使用无血清或低血清培养基，并且与在含血清培养基中培养获得的促进作用相当，对动物细胞的增殖具有促进作用。