公开(公告)号：US07892835B2

公开(公告)日：2011-02-22

申请号：US10593831

申请日：2005-03-23

申请人： Toshihiro Akaike ,  Keiichi Fukuda ,  Masato Nagaoka ,  Uichi Koshimizu

发明人： Toshihiro Akaike ,  Keiichi Fukuda ,  Masato Nagaoka ,  Uichi Koshimizu

IPC分类号： C12N15/09 ,  C12N5/06 ,  C12N5/08

CPC分类号： C12N5/0606 ,  C12N2533/50

摘要： A novel growing method is provided for pluripotent stem cells such as ES cells. The method of the invention is a pluripotent stem cell growing method and gene transfer method in which pluripotent stem cells are cultured under conditions that maintain their undifferentiated state and pluripotency, the method being characterized by using a liquid medium and a culturing vessel having immobilized or coated on a substrate solid phase surface a molecule which is adhesive to the pluripotent stem cells in a fixed concentration, to grow the pluripotent stem cells in a dispersed state while maintaining their undifferentiated state and pluripotency, without using feeder cells, or to transfer and express a gene therein.

摘要翻译： 为多能干细胞（如ES细胞）提供了一种新的生长方法。 本发明的方法是多能干细胞生长方法和基因转移方法，其中多能干细胞在保持其未分化状态和多能性的条件下培养，该方法的特征在于使用液体培养基和具有固定或涂覆的培养容器 在基质固相表面上以固定浓度与多能干细胞粘合的分子，以分散状态生长多能干细胞，同时保持其未分化状态和多能性，而不使用饲养细胞，或转移和表达 基因。

公开(公告)号：US20100189699A1

公开(公告)日：2010-07-29

申请号：US12668136

申请日：2008-07-31

申请人： Fumiyuki Hattori ,  Keiichi Fukuda

发明人： Fumiyuki Hattori ,  Keiichi Fukuda

IPC分类号： A61K35/34 ,  C12N5/071 ,  A61P9/00

CPC分类号： A61K35/34 ,  A61K35/12 ,  A61L27/3826 ,  A61L27/3895 ,  A61L2430/20 ,  C12N5/0657 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/135 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2506/00 ,  C12N2506/03 ,  C12N2506/45

摘要： The object of the present invention is to improve the post-transplantation engraftment rate of cardiomyocytes that have been purified to such an extent that they are free from non-cardiomyocytes and any components derived from other species.To solve this problem, the present inventors studied the possibility of constructing cell masses from the purified cardiomyocytes. As a result, they revealed that the stated problem could be solved by providing a method of preparing cell masses of cardiomyocytes derived from pluripotent stem cells, characterized in that cell masses of aggregated cells containing cardiomyocytes that had been differentiated and induced from pluripotent stem cells were dispersed to single cells to thereby obtain purified cardiomyocytes, which were then cultured in a culture medium under serum-free conditions so that they were reaggregated.

摘要翻译： 本发明的目的是改善已经被纯化到它们不含非心肌细胞和来自其它物种的任何成分的程度的心肌细胞的移植后植入率。 为了解决这个问题，本发明人研究了从纯化的心肌细胞构建细胞团的可能性。 结果表明，通过提供一种制备多能干细胞衍生的心肌细胞群的方法，可以解决上述问题，其特征在于分化并诱导多能干细胞的含有心肌细胞的聚集细胞的细胞团是 分散到单细胞，从而获得纯化的心肌细胞，然后将其在无血清条件下在培养基中培养，使其再聚集。

公开(公告)号：US20100145441A1

公开(公告)日：2010-06-10

申请号：US12067593

申请日：2006-09-15

申请人： Keiichi Fukuda ,  Yuji Hiraki

发明人： Keiichi Fukuda ,  Yuji Hiraki

IPC分类号： A61F2/24 ,  A61K38/17 ,  A61K31/711 ,  A61K49/00 ,  A61P35/04 ,  A61P9/10 ,  A61P27/02 ,  A61P9/00 ,  A01K67/027 ,  C12Q1/02 ,  C12Q1/68 ,  C12N5/071

CPC分类号： A61K48/005 ,  A01K2217/075 ,  A01K2267/03 ,  A61K31/7088 ,  A61K38/00 ,  C07K14/51 ,  G01N33/502 ,  G01N2800/32

摘要： An objective of the present invention is to analyze the function of anti-angiogenic factors in cardiac valves or such to elucidate the developmental mechanism of angiogenesis-induced diseases. A more specific objective is to provide therapeutic agents for angiogenesis-induced diseases such as valvular heart disease, and methods of efficiently screening for the therapeutic agents. The present inventors discovered that chondromodulin-I was markedly expressed in cardiac valves, and plays an important role in maintaining normal functions of the valves by preventing angiogenesis, thickening, and calcification which lead to valvular heart diseases. Chondromodulin-I proteins and substances that activate the expression or function of the proteins are expected to have therapeutic effects against angiogenesis-induced diseases.

摘要翻译： 本发明的目的是分析心脏瓣膜中抗血管生成因子的功能，从而阐明血管生成诱发疾病的发展机制。 更具体的目的是提供血管发生诱导的疾病如瓣膜性心脏病的治疗剂和有效筛选治疗剂的方法。 本发明人发现软骨调节蛋白-1在心脏瓣膜中显着表达，并且通过预防血管生成，增厚和钙化导致瓣膜性心脏病的维持正常功能起重要作用。 软骨调节蛋白-1蛋白质和激活蛋白质表达或功能的物质预计具有对血管生成诱导的疾病的治疗作用。

公开(公告)号：US20090275132A1

公开(公告)日：2009-11-05

申请号：US12162684

申请日：2007-01-31

申请人： Fumiyuki Hattori ,  Keiichi Fukuda

发明人： Fumiyuki Hattori ,  Keiichi Fukuda

IPC分类号： C12N5/06

CPC分类号： C12N5/0657 ,  C12N2500/02 ,  C12N2500/14 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2501/70 ,  C12N2506/02

摘要： An object of the present invention is to develop a method for purify cardiomyocytes at a high degree of purification and at a high yield from a cell mixture comprising cardiomyocytes derived from fetuses and stem cells using various features which have not been previously expected to be used for purification of cardiomyocytes or which are newly found, wherein said method is carried out without undergoing any genetic modification or without adding any special proteins or biologically active agents.The inventors of the present invention found that cardiomyocytes were effectively and highly selected and purified by culturing cardiomyocytes derived from embryonic stem cells in the culture medium under a condition selected from a low-serum-supplemented condition, a low-glucose-supplemented condition, a low-nutritional condition, a low calcium condition, a mildly-acidic pH condition, a lactic acid-supplemented condition, an aspartic acid/glutamic acid-supplemented condition, and/or a pyruvic acid-supplemented condition. The inventors of the present invention further found that the above method invented in relation to embryonic stem cells was applicable to select and purify cardiomyocytes derived from fetuses or adult stem cells.

摘要翻译： 本发明的一个目的是开发一种以高度纯化和高产率从包含来自胎儿和干细胞的心肌细胞的细胞混合物中纯化心肌细胞的方法，该方法使用先前预期不会用于 纯化心肌细胞或新发现的，其中所述方法进行而不经历任何遗传修饰或不添加任何特殊的蛋白质或生物活性剂。 本发明人发现，通过在选自低血清补充条件，低葡萄糖补充条件，细菌培养条件下的培养基中培养来源于培养基中的胚胎干细胞的心肌细胞，有效地和高度地选择和纯化心肌细胞 低营养状态，低钙状态，轻度酸性pH条件，补充乳酸的条件，天冬氨酸/谷氨酸补充条件和/或丙酮酸补充条件。 本发明人进一步发现，关于胚胎干细胞发明的上述方法适用于选择和纯化来源于胎儿或成体干细胞的心肌细胞。

公开(公告)号：US07303337B2

公开(公告)日：2007-12-04

申请号：US10628777

申请日：2003-07-28

申请人： Takeshi Fujimoto ,  Yoshiki Kuhara ,  Hiromi Nakanishi ,  Keiichi Fukuda

发明人： Takeshi Fujimoto ,  Yoshiki Kuhara ,  Hiromi Nakanishi ,  Keiichi Fukuda

IPC分类号： G02B6/36 ,  G02B6/00

CPC分类号： G02B6/4201 ,  G02B6/4256 ,  G02B6/4269 ,  H01L2224/45144 ,  H01L2224/48091 ,  H05K1/0203 ,  H05K1/14 ,  H05K3/0058 ,  H01L2924/00014 ,  H01L2924/00

摘要： In an optical communications module comprising one or more dielectric substrates equipped with an optical transmitter section, an optical receiver section or an optical transceiver section, and a chassis encasing all of them, a metal part is formed on one side of at least one of the dielectric substrates such that the metal part constitutes the whole or a part of the exterior surface of the chassis so that the length of heat conduction from a dielectric substrate with heat-generating components installed thereon can be minimized so as to efficiently release heat through the metal part to outside, curbing a temperature increase within the chassis. Thus, the optical communications module can exhibit excellent performance.

摘要翻译： 在包括配备有光发送器部分，光接收器部分或光收发器部分的一个或多个电介质基板的光通信模块以及包含其中的全部的基座的情况下，金属部件形成在至少一个 电介质基板，使得金属部分构成底盘的外表面的全部或一部分，使得可以使来自具有安装在其上的发热部件的电介质基板的热传导的长度最小化，以便有效地通过金属释放热量 部分到外面，遏制底盘内的温度升高。 因此，光通信模块可以表现出优异的性能。

公开(公告)号：US20070134215A1

公开(公告)日：2007-06-14

申请号：US10574530

申请日：2004-10-04

申请人： Keiichi Fukuda ,  Shinsuke Yuasa ,  Hideyuki Okano ,  Takuya Shimazaki ,  Uichi Koshimizu ,  Tomofumi Tanaka ,  Keijiro Sugimura

发明人： Keiichi Fukuda ,  Shinsuke Yuasa ,  Hideyuki Okano ,  Takuya Shimazaki ,  Uichi Koshimizu ,  Tomofumi Tanaka ,  Keijiro Sugimura

IPC分类号： A61K48/00 ,  C12N5/08

CPC分类号： C12N5/0657 ,  C12N2501/155 ,  C12N2506/03

摘要： For a method of inducing differentiation of cardiomyocytes from stem cells, a method is provided to induce efficiently and selectively differentiation of cardiomyocytes by such a method in which the stem cells are cultured to induce differentiation into cardiomyocytes in the presence of a substance that inhibits BMP signaling.

摘要翻译： 为了诱导心肌细胞从干细胞分化的方法，提供了一种通过这样一种方法诱导心肌细胞的有效和选择性分化的方法，其中培养干细胞以在抑制BMP信号的物质存在下诱导分化成心肌细胞 。

公开(公告)号：US20070081970A1

公开(公告)日：2007-04-12

申请号：US10577241

申请日：2004-10-27

申请人： Keiichi Fukuda ,  Jun Fujita

发明人： Keiichi Fukuda ,  Jun Fujita

IPC分类号： A61K38/19

CPC分类号： A61K38/193

摘要： The object of the present invention is to achieve simple recruitment of fibroblasts into wounded tissues and engraftment of the fibroblasts in the wounded tissues, thereby healing the wounds without requiring fibroblast transplantation. Upon G-CSF administration, fibroblasts migrate into myocardial infarct lesions and prevent a reduction in cardiac function, thus alleviating cardiac remodeling.

摘要翻译： 本发明的目的是实现将伤口组织中的成纤维细胞简单地募集到受伤组织中的成纤维细胞，从而使伤口愈合而不需要成纤维细胞移植。 在G-CSF施用后，成纤维细胞迁移到心肌梗塞病变中并防止心脏功能的降低，从而减轻心脏重塑。

公开(公告)号：US20060281173A1

公开(公告)日：2006-12-14

申请号：US10572453

申请日：2004-03-25

申请人： Keiichi Fukuda ,  Yuji Itabashi ,  Kazuo Tsubota

发明人： Keiichi Fukuda ,  Yuji Itabashi ,  Kazuo Tsubota

IPC分类号： C12N5/06 ,  C12N5/00 ,  A61K35/12

CPC分类号： C12N5/0068 ,  C12N2533/56

摘要： Disclosed are a method for manufacturing a cell sheet, comprising culturing cells on a fibrin-coated surface of a substrate until the cells reach confluency, continuing the cultivation of the cells for a sufficient time period to cause the degradation of fibrin at the bottom of the cells, and detaching the cultured cells from the substrate surface in a sheet-like form to give a cell sheet; a substrate for cell sheet preparation, a surface of which is coated with fibrin; and a composition for use in coating with fibrin a surface of a substrate for cell sheet preparation, the composition comprising fibrinogen and thrombin. The invention enables to cell sheets to be manufactured by a simple manipulation using a substrate coated with a commercial, commonly available material of which safety has been confirmed.

摘要翻译： 公开了一种制造细胞片的方法，包括在基质的纤维蛋白包被的表面上培养细胞直到细胞达到融合，继续培养细胞足够的时间以引起纤维蛋白降解在 细胞，并以片状形式将培养的细胞从基底表面分离，得到细胞片; 用于细胞片制备的基底，其表面涂有纤维蛋白; 以及用于细胞片制备用底物的表面纤维蛋白的涂布用组合物，所述组合物含有纤维蛋白原和凝血酶。 本发明使得能够通过简单的操作使用涂覆有已经确认了安全性的商业的通常可获得的材料的基材来制造细胞片。

公开(公告)号：US07049560B2

公开(公告)日：2006-05-23

申请号：US10813170

申请日：2004-03-31

申请人： Keiichi Fukuda ,  Yoshiyuki Motoyoshi

发明人： Keiichi Fukuda ,  Yoshiyuki Motoyoshi

IPC分类号： H05B3/34

CPC分类号： H05B3/34 ,  H05B2203/017

摘要： A tape heater includes an electric heating wire disposed on a surface of a heat-resistant, flexible substrate strip. The electric heating wire and the substrate strip are wrapped with a heat-resistant resin wrap.

摘要翻译： 带式加热器包括设置在耐热柔性衬底条的表面上的电加热线。 电热丝和基材条用耐热树脂包裹包裹。

公开(公告)号：US20050067038A1

公开(公告)日：2005-03-31

申请号：US10949520

申请日：2004-09-27

申请人： Tsuyoshi Kobayashi ,  Yoshiyuki Motoyoshi ,  Keiichi Fukuda

发明人： Tsuyoshi Kobayashi ,  Yoshiyuki Motoyoshi ,  Keiichi Fukuda

IPC分类号： F16L59/147 ,  F16L59/02 ,  F16L59/14 ,  H05B3/58 ,  F16L9/14

CPC分类号： F16L59/022 ,  F16L59/026 ,  H05B3/56 ,  H05B3/565 ,  Y02B30/26

摘要： There are provided a heat insulating tool kit for piping, which can easily be attached to and detached from a piping in a limited space repeatedly and fixed in a stable form, can be attached so that a heating surface is in close contact with the outer peripheral surface of piping so that heat is conducted efficiently, which results in no overheating of heated portion, can be subjected to temperature control stably, and can be used in a clean room etc. because dust particles scarcely occur, and a heat insulating construction using the heat insulating tool kit. The heat insulating tool kit includes a piping; a band-shaped heat insulating unit which includes at least a heat insulating material and preferably a heating element and is wrapped with a heat-resistant resin film; a support cover consisting of a tubular element of a C-shaped cross section having a slit-shaped opening over the total length in the axial direction of the tubular element; and preferably an external cover of almost the same shape as that of the support cover. The heat insulating construction is formed as described below. The heat insulating unit is housed so that the non-heating surface of the heat insulating unit comes into contact with the tubular element inside wall of the support cover 14, and in a state in which ear portions at both ends of a heat insulating portion are pulled out of the slit-shaped opening, the butting portion at both ends of the heat insulating portion, that is, the slit-shaped opening of support cover is pressed on the piping, by which the piping is covered with the heat insulating portion and the support cover. Next, the opening portion of external cover in a widened state is pressed on the slit-shaped opening side of the support cover, by which the heat insulating unit and the support cover are collectively covered with the external cover 15.

摘要翻译： 提供了一种用于管道的隔热工具套件，其可以容易地在有限的空间中被重新连接并且以稳定的形式固定在管道上并从其中分离，可以附接到加热表面与外周边紧密接触 管道的表面使得热量有效地传导，这导致加热部分不会过热，可以稳定地进行温度控制，并且可以用于洁净室等，因为几乎不发生粉尘颗粒，并且使用 隔热工具套件 隔热工具套件包括管道; 一种带状绝热单元，其至少包括隔热材料，优选为加热元件，并且用耐热树脂膜包裹; 支撑盖，其由在管状元件的轴向方向上的总长度上具有狭缝状开口的C形横截面的管状元件构成; 并且优选地具有与支撑盖几乎相同形状的外盖。 绝热结构如下所述形成。 收纳隔热单元，使绝热单元的非加热面与支承罩14的管状元件内壁接触，在绝热部的两端的耳部为 拉出狭缝状的开口部，将绝热部的两端的对接部分即支撑盖的狭缝状的开口按压在管道上，管道被绝热部分覆盖， 支撑盖。 接下来，加宽状态的外盖的开口部被按压在支撑罩的狭缝状的开口侧，隔热单元和支撑罩被外部盖15共同地覆盖。