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    POLYANION FOR IMPROVED NUCLEIC ACID AMPLIFICATION
    53.
    发明申请
    POLYANION FOR IMPROVED NUCLEIC ACID AMPLIFICATION 有权
    改善核酸扩增的多糖

    公开(公告)号：US20100255547A1

    公开(公告)日：2010-10-07

    申请号：US12547013

    申请日：2009-08-25

    申请人： Waltraud Ankenbauer ,  Dieter Heindl ,  Frank Laue ,  Eva Walter ,  Renate Kolb

    发明人： Waltraud Ankenbauer ,  Dieter Heindl ,  Frank Laue ,  Eva Walter ,  Renate Kolb

    IPC分类号： C12P19/34 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C12Q1/6848 ,  C12Q2527/125

    摘要： The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3≦m≦6, 30≦n≦60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an —OH group or a phosphate group, and further provided that the terminal Y can also be an —OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids.

    摘要翻译： 本发明涉及一种包含结构[Xx-（CH2）m-磷酸-Yy] n结构的新化合物，其特征在于，3和nlE; m＆nlE; 6,30＆lt; n; n＆nlE; 60，每个x和y各自独立地表示 其他0或1，每个X和Y彼此独立地具有任何光度测量实体; 条件是末端X也可以是-OH基或磷酸基，并且进一步规定，末端Y也可以是-OH基。 这种化合物可以用作基于PCR扩增核酸的合适的热启动添加剂。

    Polynucleotide labeling reagent
    54.
    发明授权
    Polynucleotide labeling reagent 有权
    多核苷酸标记试剂

    公开(公告)号：US07795423B2

    公开(公告)日：2010-09-14

    申请号：US12123656

    申请日：2008-05-20

    申请人： Dieter Heindl

    发明人： Dieter Heindl

    IPC分类号： C07H21/00

    CPC分类号： C07H21/00 ,  Y02P20/55

    摘要： The present invention provides a new labeling reagent for preparing modified oligonucleotides and processes for their production wherein these oligonucleotides contain at least once the structure P═N—SO2-benzole-L-M-X, characterized in that L is either —(CH2)n- or polyethylene glycol, M is selected from a group consisting of —NH—, —O—, —S—, and —COO—, and X is either a protecting group or a detectable unit. L is preferably either —(CH2)n- or polyethylene glycol.

    摘要翻译： 本发明提供了用于制备修饰的寡核苷酸的新标记试剂及其制备方法，其中这些寡核苷酸至少含有一种结构P = N-SO 2 - 苯并-MXX，其特征在于L为 - （CH 2）n - 或聚乙烯 乙二醇，M选自-NH-，-O-，-S-和-COO-，X是保护基或可检测单元。 L优选为 - （CH 2）n - 或聚乙二醇。

    Fret process
    58.
    发明申请
    Fret process 审中-公开
    反应过程

    公开(公告)号：US20070184453A1

    公开(公告)日：2007-08-09

    申请号：US10678440

    申请日：2003-10-01

    申请人： Gregor Sagner ,  Dieter Heindl ,  Ingrid Bechler ,  Christina Krause

    发明人： Gregor Sagner ,  Dieter Heindl ,  Ingrid Bechler ,  Christina Krause

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6818

    摘要： The present invention is directed to hybridization probes hybridizing adjacently to another at a target nucleic acid sequence, wherein one member of said hybridization probes comprises (i) a nucleotide sequence entity which is substantially complementary to the sequence of the target nucleic acid, (ii) a fluorescent entity being either a FRET donor entity or a FRET acceptor entity, and (iii) a spacer entity connecting the nucleotide sequence entity and the fluorescent entity.

    摘要翻译： 本发明涉及在目标核酸序列上与另一种相邻地杂交的杂交探针，其中所述杂交探针的一个成员包含（i）与靶核酸序列基本上互补的核苷酸序列实体，（ii） 荧光实体是FRET供体实体或FRET受体实体，和（iii）连接核苷酸序列实体和荧光实体的间隔实体。

    Labeling reagent
    59.
    发明申请
    Labeling reagent 有权
    标记试剂

    公开(公告)号：US20060216736A1

    公开(公告)日：2006-09-28

    申请号：US11370527

    申请日：2006-03-08

    申请人： Dieter Heindl ,  Thomas Froehlich ,  Heribert Maerz ,  Monika Seller

    发明人： Dieter Heindl ,  Thomas Froehlich ,  Heribert Maerz ,  Monika Seller

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C12P19/34 ,  C07H19/22

    CPC分类号： C07H19/23 ,  C07H21/00

    摘要： The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of, e.g., optimized hybridization probes, simple probes, TAQMAN-probes or molecular beacon probes.

    摘要翻译： 本发明将取代的硝基吲哚核苷作为两个末端以及用于核酸检测，分析和定量的标记的寡核苷酸探针的内部构建块。 取代基包括接头和可检测的基团或接头以及用于后合成偶联的反应性基团。 这些修饰的核苷允许进入广泛的应用领域。 这些新的取代的硝基吲哚核苷可以用作易于制备例如优化的杂交探针，简单探针，TAQMAN探针或分子信标探针的标记试剂。