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51.发明授权Microscope apparatus chronologically storing different types of image information 有权显微镜仪器按时间顺序存储不同类型的图像信息公开(公告)号：US08994806B2
公开(公告)日：2015-03-31
申请号：US12828342
申请日：2010-07-01
申请人： Mitsuhiro Nakano , Tatsuo Nakata
发明人： Mitsuhiro Nakano , Tatsuo Nakata
CPC分类号： G02B21/367
摘要： A microscope apparatus organizes and stores a plurality of types of image information acquired by a plurality of image acquisition methods at different timings. The microscope apparatus includes a time counting unit for counting time, a plurality of different image acquisition units, and a storage unit for storing image information, when acquired by any one of the image acquisition units, and timing information counted by the time counting unit, by having them associated with each other. Even if the image acquisition units acquire different types of image information at different timings, the timing information can be used to call up the image information stored in the storage unit in a chronological order.
摘要翻译： 显微镜装置以不同的定时组织并存储通过多个图像获取方法获取的多种类型的图像信息。 显微镜装置包括用于计数时间的计时单元，多个不同的图像获取单元，以及用于存储图像信息的存储单元，当由图像获取单元中的任何一个获取时，以及由计时单元计数的定时信息， 通过使它们彼此相关联。 即使图像获取单元在不同的定时获取不同类型的图像信息，也可以使用定时信息来按照时间顺序调用存储在存储单元中的图像信息。
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公开(公告)号：US08730574B2
公开(公告)日：2014-05-20
申请号：US12955428
申请日：2010-11-29
申请人： Akinori Araya , Tatsuo Nakata
发明人： Akinori Araya , Tatsuo Nakata
CPC分类号： G02B21/0032 , G02B21/00 , G02B21/0004 , G02B21/0092 , G02B21/06 , G02B21/088 , G02B21/14 , G02B21/26 , G02B21/30 , G02B21/33
摘要： A microscope system includes a culture unit for holding and cultivating a specimen while maintaining constant temperature and humidity; a stage for holding the culture unit; a first light-converging optical system for converging illumination light emitted from a light source on the specimen; a second light-converging optical system for converging transmitted light that has passed through the specimen; a transmitted-light pinhole provided at a position optically conjugate to the light-converging position of illumination light on the specimen to cut off part of transmitted light converged by the second light-converging optical system; a transmitted-light detector that detects transmitted light that has passed through the transmitted-light pinhole; a moving system for moving the first and second light-converging optical systems, the transmitted-light pinhole, and the transmitted-light detector and the stage relative to each other; a housing that encloses these components and cuts off external light; and a control unit.
摘要翻译： 显微镜系统包括用于在保持恒温恒湿的同时保持和培养样本的培养单元; 举办文化单位的舞台; 第一聚光光学系统，用于将从光源发射的照明光聚焦在所述样本上; 用于会聚穿过所述样本的透射光的第二聚光光学系统; 透射光针孔，设置在与所述样本上的照明光的聚光位置光学共轭的位置处，以切断由所述第二聚光光学系统会聚的透射光的一部分; 透射光检测器，其检测穿过透光针孔的透射光; 用于相对于彼此移动第一和第二聚光光学系统，透射光针孔和透射光检测器和台的移动系统; 封闭这些部件并切断外部光的外壳; 和控制单元。
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公开(公告)号：US08159663B2
公开(公告)日：2012-04-17
申请号：US12469007
申请日：2009-05-20
CPC分类号： G01N21/6458 , G01N21/65 , G01N2021/653 , G01N2021/656
摘要： To enable both observations of coherent anti-Stokes Raman scattering light and multiphoton fluorescence in a same apparatus so as to observe a specimen by various observation methods. There is provided a laser microscope apparatus comprising: two optical paths for guiding pulsed laser beams having two different frequencies whose frequency difference is approximately equal to a specific molecular vibration frequency in a specimen; a multiplexer for combining the pulsed laser beams guided through these two optical paths; and a frequency dispersion adjuster which is provided on at least one of these two optical paths, and is capable of adjustment to approximately equalize frequency dispersion quantities of the pulsed laser beams guided through the two optical paths.
摘要翻译： 为了使相同装置中的相干反斯托克斯拉曼散射光和多光子荧光观察到，可以通过各种观察方法观察样品。 提供了一种激光显微镜装置，包括：两个光路，用于引导具有两个不同频率的脉冲激光束，其频率差近似等于样本中的特定分子振动频率; 用于组合通过这两个光路引导的脉冲激光束的多路复用器; 以及频率分散调整器，其被设置在这两个光路中的至少一个上，并且能够调节近似均衡通过两个光路引导的脉冲激光束的频率分散量。
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公开(公告)号：US07969653B2
公开(公告)日：2011-06-28
申请号：US12002508
申请日：2007-12-17
申请人： Akinori Araya , Tatsuo Nakata , Makio Ueno
发明人： Akinori Araya , Tatsuo Nakata , Makio Ueno
CPC分类号： G02B21/0036
摘要： It is possible to achieve a required field number and numerical aperture for microscope observation at a scanning speed equal to video-rate or higher and also to change the scanning speed with a simple configuration. The invention provides a laser microscope including a laser light source; a scanning unit configured to scan a specimen with laser light emitted from the laser light source; and an objective lens configured to focus the laser light scanned by the scanning unit on the specimen. The scanning unit is provided with an electro-optical deflecting element including an electro-optical crystal in which a refractive index gradient is induced by injecting electric current.
摘要翻译： 可以以等于视频速率或更高的扫描速度实现用于显微镜观察的所需场数和数值孔径，并且还可以以简单的配置改变扫描速度。 本发明提供一种包括激光光源的激光显微镜; 扫描单元，被配置为用从所述激光光源发射的激光扫描样本; 以及配置为将由扫描单元扫描的激光聚焦在样本上的物镜。 扫描单元设置有电光偏转元件，其包括通过注入电流而引起折射率梯度的电光晶体。
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公开(公告)号：US20110134516A1
公开(公告)日：2011-06-09
申请号：US12955428
申请日：2010-11-29
申请人： Akinori ARAYA , Tatsuo Nakata
发明人： Akinori ARAYA , Tatsuo Nakata
CPC分类号： G02B21/0032 , G02B21/00 , G02B21/0004 , G02B21/0092 , G02B21/06 , G02B21/088 , G02B21/14 , G02B21/26 , G02B21/30 , G02B21/33
摘要： Disclosed herein is a microscope system. The microscope system includes a culture unit for holding and cultivating a specimen while maintaining constant temperature and humidity; a stage for holding the culture unit; a first light-converging optical system for converging illumination light emitted from a light source on the specimen; a second light-converging optical system for converging transmitted light that has passed through the specimen; a transmitted-light pinhole provided at a position optically conjugate to the light-converging position of illumination light on the specimen to cut off part of transmitted light converged by the second light-converging optical system; a transmitted-light detector that detects transmitted light that has passed through the transmitted-light pinhole; a moving system for moving the first and second light-converging optical systems, the transmitted-light pinhole, and the transmitted-light detector and the stage relative to each other; a housing that encloses these components and cuts off external light; and a temperature control unit for controlling temperature in the housing.
摘要翻译： 本文公开的是显微镜系统。 显微镜系统包括用于在保持恒温恒湿的同时保持和培养样本的培养单元; 举办文化单位的舞台; 第一聚光光学系统，用于将从光源发射的照明光聚焦在所述样本上; 用于会聚穿过所述样本的透射光的第二聚光光学系统; 透射光针孔，设置在与所述样本上的照明光的聚光位置光学共轭的位置处，以切断由所述第二聚光光学系统会聚的透射光的一部分; 透射光检测器，其检测穿过透光针孔的透射光; 用于相对于彼此移动第一和第二聚光光学系统，透射光针孔和透射光检测器和台的移动系统; 封闭这些部件并切断外部光的外壳; 以及用于控制壳体中的温度的温度控制单元。
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公开(公告)号：US20100294949A1
公开(公告)日：2010-11-25
申请号：US12784643
申请日：2010-05-21
申请人： Hiroshi SASAKI , Tatsuo Nakata
发明人： Hiroshi SASAKI , Tatsuo Nakata
CPC分类号： G02B21/0076
摘要： Spectrum detection is performed at a high S/N ratio, high sensitivity, and high speed. Provided is a scanning microscope device that includes a light source that irradiates a sample with laser light; an X-Y galvanometer mirror that scans the laser light from the light source on the sample; an objective lens that irradiates the sample with the laser light scanned by the X-Y galvanometer mirror and collects fluorescence generated at an irradiated position of the laser light; a non-descan-detection excitation DM that is disposed between the X-Y galvanometer mirror and the objective lens and separates the laser light and the fluorescence from each other; an fiber for epi-fluorescence that receives the separated fluorescence through an entrance end thereof, guides the fluorescence, and emits the fluorescence from an exit end thereof that is formed in a substantially linear shape; a diffraction grating that disperses the fluorescence emitted from the exit end of the fiber for epi-fluorescence in a direction orthogonal to the longitudinal direction of the exit end; and a multi-anode PMT having a plurality of cells arrayed in the dispersing direction of the fluorescence dispersed by the diffraction grating.
摘要翻译： 频谱检测以高S / N比，高灵敏度和高速进行。 提供了一种扫描显微镜装置，其包括用激光照射样品的光源; 用于扫描来自样品上的光源的激光的X-Y电流计镜; 物镜，用X-Y电流计镜扫描的激光照射样品，并收集在激光的照射位置产生的荧光; 设置在X-Y电流计反射镜和物镜之间并将激光和荧光彼此分离的非除数检测激励DM; 通过其入射端接收分离的荧光的外荧光纤维引导荧光，并从形成为大致线形的出射端发射荧光; 衍射光栅，其在与所述出射端的纵向方向正交的方向上分散从所述光纤的出射端发射的荧光以进行荧光荧光; 以及多个阳极PMT，其具有以由衍射光栅分散的荧光的分散方向排列的多个单元。
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公开(公告)号：US07485876B2
公开(公告)日：2009-02-03
申请号：US11711407
申请日：2007-02-27
申请人： Masaharu Tomioka , Tatsuo Nakata , Hiroshi Sasaki
发明人： Masaharu Tomioka , Tatsuo Nakata , Hiroshi Sasaki
IPC分类号： G01J1/58
CPC分类号： G02B21/0076 , G02B21/0064 , G02B21/0068
摘要： A laser scanning microscope includes an exciting observation light source, a stimulation light source, a first scanning portion that two-dimensionally scans the exciting observation light across a specimen, a second scanning portion that sets an incident position of the stimulation light on the specimen, an optical path combining portion that brings the exciting observation light and the stimulation light to an identical optical path, an objective lens that irradiates the specimen with the exciting observation light and/or the stimulation light while collecting fluorescence light emitted from the specimen, and a detecting portion that detects the collected fluorescence light, the optical path combining portion being disposed so as to be in an optically conjugate relationship with the first scanning portion and the second scanning portion.
摘要翻译： 激光扫描显微镜包括激发观察光源，刺激光源，二维地扫描穿过样本的激发观察光的第一扫描部分，将刺激光的入射位置设定在样本上的第二扫描部， 将激发观察光和刺激光带入相同的光路的光路组合部，在收集从样本发出的荧光的同时照射具有激发观察光和/或刺激光的样本的物镜， 检测部分，其检测所收集的荧光，所述光路组合部分设置成与所述第一扫描部分和所述第二扫描部分处于光学共轭关系。
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公开(公告)号：US07456378B2
公开(公告)日：2008-11-25
申请号：US11805378
申请日：2007-05-22
申请人： Hiroshi Sasaki , Eiji Yokoi , Tatsuo Nakata
发明人： Hiroshi Sasaki , Eiji Yokoi , Tatsuo Nakata
CPC分类号： G02B21/16 , G02B21/0032 , G02B21/004
摘要： The invention provides a confocal microscope comprising a light source; a light scanning unit; an array device; a line-beam generating unit for imaging illumination light in the form of a straight line extending, on the array device, in a direction intersecting the scanning direction of the light scanning unit; an objective lens for imaging the illumination light reflected or transmitted at the array device on a specimen; a beamsplitter, between the array device and the light scanning unit, for splitting off from the illumination light detection light from the specimen; a two-dimensional image-acquisition unit for acquiring the split off detection light; and a control unit for controlling the light scanning unit and the array device, wherein the array device is disposed in an optically conjugate positional relationship with a focal plane of the objective lens, and the control unit performs control so as to synchronize the light scanning unit and the array device.
摘要翻译： 本发明提供一种包括光源的共焦显微镜; 光扫描单元; 阵列器件; 一个线束产生单元，用于在阵列装置上沿与光扫描单元的扫描方向相交的方向成直线的形式的照明光; 用于对在样品上的阵列装置反射或透射的照明光进行成像的物镜; 阵列器件和光扫描单元之间的分束器，用于从来自样本的照明光检测光分离; 用于获取分离检测光的二维图像获取单元; 以及控制单元，用于控制光扫描单元和阵列器件，其中阵列器件与物镜的焦平面以光学共轭位置关系设置，并且控制单元执行控制以使光扫描单元 和阵列设备。
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公开(公告)号：US20080252967A1
公开(公告)日：2008-10-16
申请号：US12060994
申请日：2008-04-02
IPC分类号： G02B21/34
CPC分类号： G02B21/33
摘要： An image can be prevented from becoming unclear over time during long-term observation. The invention provides a microscope apparatus including a specimen container for containing a specimen; an objective lens disposed opposite the specimen container for collecting light from the specimen in the specimen container; an immersion-liquid supplying unit for supplying immersion liquid to a space between the objective lens and the specimen container; and an immersion-liquid removing unit for removing the immersion liquid from the space between the objective lens and the specimen container. The immersion-liquid removing unit includes a nozzle for ejecting compressed air to the space between the objective lens and the specimen container.
摘要翻译： 在长期观察期间，可以防止图像变得不清楚。 本发明提供了一种显微镜装置，其包括：容纳样本的检体容器; 与样本容器相对设置的用于收集来自样本容器中的样本的光的物镜; 浸没液供给单元，其向所述物镜和所述检体容器之间的空间供给浸渍液; 以及用于从物镜和样本容器之间的空间中去除浸液的浸液清除单元。 浸液清除单元包括用于将压缩空气喷射到物镜和样本容器之间的空间的喷嘴。
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公开(公告)号：US20080247038A1
公开(公告)日：2008-10-09
申请号：US12058956
申请日：2008-03-31
申请人： Hiroshi SASAKI , Tatsuo Nakata
发明人： Hiroshi SASAKI , Tatsuo Nakata
IPC分类号： G02B21/26
CPC分类号： G02B21/0088 , G02B21/0024 , G02B21/26 , G02B21/30 , G02B21/33 , G02B21/34
摘要： A scanning confocal microscope can provide an image while preventing a decrease in brightness and blurring due to strain caused in optical and mechanical systems by thermal effects in a high-temperature, high-humidity incubation container. This scanning confocal microscope includes an incubation container that has a space in which a specimen is disposed and that can maintain an internal environment thereof at a predetermined temperature and high humidity and an optical system space adjacent to the incubation container and separated therefrom based on humidity. The optical system space accommodates a light-scanning unit and a scanner optical system, an objective lens, a confocal pinhole, and a focus adjustment mechanism. The optical system space further accommodates a temperature-maintaining unit for the optical system space to maintain the optical system space at a temperature substantially equal to the temperature in the incubation container.
摘要翻译： 扫描共焦显微镜可以提供图像，同时防止在高温，高湿度孵育容器中通过热效应在光学和机械系统中引起的应变引起的亮度降低和模糊。 该扫描共聚焦显微镜包括具有放置试样的空间并且能够将其内部环境保持在预定温度和高湿度的空间以及与培养容器相邻的光学系统空间并基于湿度而分离的孵育容器。 光学系统空间容纳光扫描单元和扫描仪光学系统，物镜，共焦针孔和焦点调节机构。 光学系统空间还容纳用于光学系统空间的温度保持单元，以将光学系统空间保持在基本上等于孵化容器中的温度的温度。
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