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公开(公告)号：US07485876B2
公开(公告)日：2009-02-03
申请号：US11711407
申请日：2007-02-27
申请人： Masaharu Tomioka , Tatsuo Nakata , Hiroshi Sasaki
发明人： Masaharu Tomioka , Tatsuo Nakata , Hiroshi Sasaki
IPC分类号： G01J1/58
CPC分类号： G02B21/0076 , G02B21/0064 , G02B21/0068
摘要： A laser scanning microscope includes an exciting observation light source, a stimulation light source, a first scanning portion that two-dimensionally scans the exciting observation light across a specimen, a second scanning portion that sets an incident position of the stimulation light on the specimen, an optical path combining portion that brings the exciting observation light and the stimulation light to an identical optical path, an objective lens that irradiates the specimen with the exciting observation light and/or the stimulation light while collecting fluorescence light emitted from the specimen, and a detecting portion that detects the collected fluorescence light, the optical path combining portion being disposed so as to be in an optically conjugate relationship with the first scanning portion and the second scanning portion.
摘要翻译： 激光扫描显微镜包括激发观察光源，刺激光源，二维地扫描穿过样本的激发观察光的第一扫描部分，将刺激光的入射位置设定在样本上的第二扫描部， 将激发观察光和刺激光带入相同的光路的光路组合部，在收集从样本发出的荧光的同时照射具有激发观察光和/或刺激光的样本的物镜， 检测部分，其检测所收集的荧光，所述光路组合部分设置成与所述第一扫描部分和所述第二扫描部分处于光学共轭关系。
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公开(公告)号：US20070205378A1
公开(公告)日：2007-09-06
申请号：US11711407
申请日：2007-02-27
申请人： Masaharu Tomioka , Tatsuo Nakata , Hiroshi Sasaki
发明人： Masaharu Tomioka , Tatsuo Nakata , Hiroshi Sasaki
CPC分类号： G02B21/0076 , G02B21/0064 , G02B21/0068
摘要： A laser scanning microscope provides advantages that replacement of any optical element becomes unnecessary, and ease of operation and positioning repeatability of light stimulation are improved. The laser scanning microscope includes an exciting observation light source, a stimulation light source, a first scanning portion that two-dimensionally scans the exciting observation light across a specimen, a second scanning portion that sets an incident position of the stimulation light on the specimen, an optical path combining portion that brings the exciting observation light and the stimulation light to an identical optical path, an objective lens that irradiates the specimen with the exciting observation light and/or the stimulation light while converging fluorescence light emitted from the specimen, and a detecting portion that detects the converged fluorescence light, the optical path combining portion being disposed so as to be in an optically conjugate relationship with the first scanning portion and the second scanning portion.
摘要翻译： 激光扫描显微镜提供了不需要更换任何光学元件的优点，并且提高了光刺激的操作和定位重复性的容易性。 激光扫描显微镜包括激发观察光源，刺激光源，二维地扫描穿过样本的激发观察光的第一扫描部分，将刺激光的入射位置设定在样本上的第二扫描部， 将激发观察光和刺激光带到相同的光路的光路组合部，使从激光观察光和/或刺激光照射到样本的物镜同时会聚从样本发出的荧光，以及 检测所述会聚的荧光的检测部，所述光路组合部被配置为与所述第一扫描部和所述第二扫描部成为光学共轭关系。
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公开(公告)号：US07456378B2
公开(公告)日：2008-11-25
申请号：US11805378
申请日：2007-05-22
申请人： Hiroshi Sasaki , Eiji Yokoi , Tatsuo Nakata
发明人： Hiroshi Sasaki , Eiji Yokoi , Tatsuo Nakata
CPC分类号： G02B21/16 , G02B21/0032 , G02B21/004
摘要： The invention provides a confocal microscope comprising a light source; a light scanning unit; an array device; a line-beam generating unit for imaging illumination light in the form of a straight line extending, on the array device, in a direction intersecting the scanning direction of the light scanning unit; an objective lens for imaging the illumination light reflected or transmitted at the array device on a specimen; a beamsplitter, between the array device and the light scanning unit, for splitting off from the illumination light detection light from the specimen; a two-dimensional image-acquisition unit for acquiring the split off detection light; and a control unit for controlling the light scanning unit and the array device, wherein the array device is disposed in an optically conjugate positional relationship with a focal plane of the objective lens, and the control unit performs control so as to synchronize the light scanning unit and the array device.
摘要翻译： 本发明提供一种包括光源的共焦显微镜; 光扫描单元; 阵列器件; 一个线束产生单元，用于在阵列装置上沿与光扫描单元的扫描方向相交的方向成直线的形式的照明光; 用于对在样品上的阵列装置反射或透射的照明光进行成像的物镜; 阵列器件和光扫描单元之间的分束器，用于从来自样本的照明光检测光分离; 用于获取分离检测光的二维图像获取单元; 以及控制单元，用于控制光扫描单元和阵列器件，其中阵列器件与物镜的焦平面以光学共轭位置关系设置，并且控制单元执行控制以使光扫描单元 和阵列设备。
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公开(公告)号：US20070272843A1
公开(公告)日：2007-11-29
申请号：US11805378
申请日：2007-05-22
申请人： Hiroshi Sasaki , Eiji Yokoi , Tatsuo Nakata
发明人： Hiroshi Sasaki , Eiji Yokoi , Tatsuo Nakata
CPC分类号： G02B21/16 , G02B21/0032 , G02B21/004
摘要： The invention provides a confocal microscope comprising a light source; a light scanning unit; an array device; a line-beam generating unit for imaging illumination light in the form of a straight line extending, on the array device, in a direction intersecting the scanning direction of the light scanning unit; an objective lens for imaging the illumination light reflected or transmitted at the array device on a specimen; a beamsplitter, between the array device and the light scanning unit, for splitting off from the illumination light detection light from the specimen; a two-dimensional image-acquisition unit for acquiring the split off detection light; and a control unit for controlling the light scanning unit and the array device, wherein the array device is disposed in an optically conjugate positional relationship with a focal plane of the objective lens, and the control unit performs control so as to synchronize the light scanning unit and the array device.
摘要翻译： 本发明提供一种包括光源的共焦显微镜; 光扫描单元; 阵列器件; 一个线束产生单元，用于在阵列装置上沿与光扫描单元的扫描方向相交的方向成直线的形式的照明光; 用于对在样品上的阵列装置反射或透射的照明光进行成像的物镜; 阵列器件和光扫描单元之间的分束器，用于从来自样本的照明光检测光分离; 用于获取分离检测光的二维图像获取单元; 以及控制单元，用于控制光扫描单元和阵列器件，其中阵列器件与物镜的焦平面以光学共轭位置关系设置，并且控制单元执行控制以使光扫描单元 和阵列设备。
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公开(公告)号：US20070109634A1
公开(公告)日：2007-05-17
申请号：US11593842
申请日：2006-11-07
申请人： Makoto Araki , Hiroshi Sasaki , Tatsuo Nakata , Makio Ueno
发明人： Makoto Araki , Hiroshi Sasaki , Tatsuo Nakata , Makio Ueno
IPC分类号： G02B21/06
CPC分类号： G02B21/0032 , G01N21/6458 , G01N2021/6439 , G02B21/0044 , G02B21/0076 , G02B21/0084 , G02B21/16 , G02B21/32
摘要： A microscope apparatus that can observe into the interior of a specimen and that can apply an optical stimulus over a wide area within a short period of time is provided. The microscope apparatus comprises at least one observation scanning optical system including a laser light source for emitting observation laser light, an objective lens, and a scanning optical system for two-dimensionally scanning the observation laser light in a predetermined examination plane of the specimen via the objective lens; and at least one stimulus optical system which includes a lamp light source for emitting light having a wavelength used for optical stimulation and which irradiates the specimen with the light emitted from the lamp light source.
摘要翻译： 提供了可以观察到样本内部并且可以在短时间内在广泛区域上施加光学刺激的显微镜装置。 显微镜装置包括至少一个观察扫描光学系统，其包括用于发射观察激光的激光光源，物镜和用于在样本的预定检查平面中二维扫描观察激光的扫描光学系统 物镜; 以及至少一个刺激光学系统，其包括用于发射具有用于光学刺激的波长的光的灯光源，并且从灯光源发射的光照射样本。
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公开(公告)号：US07176428B2
公开(公告)日：2007-02-13
申请号：US11070191
申请日：2005-03-03
CPC分类号： G01N21/6486 , G01N21/6456
摘要： The invention provides a multiphoton-excitation-type examination apparatus that efficiently generates a multiphoton-excitation effect, that makes the measurement head compact, and that can be easily adjusted when the measurement head is replaced. The multiphoton-excitation-type examination apparatus comprises a laser light source that oscillates ultrashort pulsed laser light; an optical fiber that transmits the ultrashort pulsed laser light from the laser light source; a support member; a measurement head supported on the support member so as to be movable upwards and downwards and at an angle, and having an optical system that irradiates a specimen with the ultrashort pulsed laser light transmitted by the optical fiber that measures fluorescence or reflected light coming from the specimen; and a dispersion-compensating member, in the measurement head, that compensates for group velocity dispersion of the ultrashort pulsed laser light irradiated onto the specimen.
摘要翻译： 本发明提供了一种多光子激发型检查装置，其有效地产生使得测量头紧凑，并且在更换测量头时可以容易地调节的多光子激发效果。 多光子激发型检查装置包括：振荡超短脉冲激光的激光光源; 从激光光源发射超短脉冲激光的光纤; 支撑构件; 测量头支撑在支撑构件上，以便可上下移动一定角度，并且具有光学系统，该光学系统用由光纤透射的超短脉冲激光照射样本，该光纤测量荧光或来自 标本; 以及在测量头中补偿照射到样本上的超短脉冲激光的组速度色散的色散补偿部件。
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公开(公告)号：US08154796B2
公开(公告)日：2012-04-10
申请号：US11593842
申请日：2006-11-07
申请人： Makoto Araki , Hiroshi Sasaki , Tatsuo Nakata , Makio Ueno
发明人： Makoto Araki , Hiroshi Sasaki , Tatsuo Nakata , Makio Ueno
IPC分类号： G02B21/06
CPC分类号： G02B21/0032 , G01N21/6458 , G01N2021/6439 , G02B21/0044 , G02B21/0076 , G02B21/0084 , G02B21/16 , G02B21/32
摘要： A microscope apparatus that can observe into the interior of a specimen and that can apply an optical stimulus over a wide area within a short period of time is provided. The microscope apparatus comprises at least one observation scanning optical system including a laser light source for emitting observation laser light, an objective lens, and a scanning optical system for two-dimensionally scanning the observation laser light in a predetermined examination plane of the specimen via the objective lens; and at least one stimulus optical system which includes a lamp light source for emitting light having a wavelength used for optical stimulation and which irradiates the specimen with the light emitted from the lamp light source.
摘要翻译： 提供了可以观察到样本内部并且可以在短时间内在广泛区域上施加光学刺激的显微镜装置。 显微镜装置包括至少一个观察扫描光学系统，其包括用于发射观察激光的激光光源，物镜和用于在样本的预定检查平面中二维扫描观察激光的扫描光学系统 物镜; 以及至少一个刺激光学系统，其包括用于发射具有用于光学刺激的波长的光的灯光源，并且从灯光源发射的光照射样本。
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公开(公告)号：US07268344B2
公开(公告)日：2007-09-11
申请号：US11447761
申请日：2006-06-06
IPC分类号： G01J1/32
CPC分类号： G02B21/008 , G01N21/6458
摘要： A scanning laser microscope that can quantitatively display a laser irradiation power on a display unit and that can suppress fluctuations in the laser irradiation power is provided. The scanning laser microscope includes a light source configured to emit laser light, a scanning unit configured to scan the laser light emitted from the light source on a sample, a photodetector configured to detect light from the sample, a processing unit configured to convert a signal from the photodetector into an image signal of the sample and to output the image signal, a reference light detector configured to detect part of the laser light emitted from the light source as a reference light signal, a laser light control unit configured to control the power of the laser light emitted from the light source, an irradiation-power calculation unit configured to calculate an irradiation power value at the sample on the basis of the reference light signal detected by the reference light detector, and a display unit configured to display the calculated irradiation power value at the sample.
摘要翻译： 提供了一种可以在显示单元上定量显示激光照射功率并且可以抑制激光照射功率的波动的扫描激光显微镜。 该扫描激光显微镜包括配置为发射激光的光源，被配置为扫描从样品上的光源发射的激光的扫描单元，被配置为检测来自样品的光的光电检测器，被配置为将信号 从光电检测器到样品的图像信号并输出图像信号;参考光检测器，被配置为检测从光源发射的激光的一部分作为参考光信号;激光控制单元，被配置为控制功率 照射功率计算单元，被配置为基于由参考光检测器检测的参考光信号计算样本处的照射功率值;以及显示单元，被配置为显示所计算的 样品照射功率值。
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公开(公告)号：US20070120070A1
公开(公告)日：2007-05-31
申请号：US11647031
申请日：2006-12-27
IPC分类号： G01J1/58
CPC分类号： G01N21/6486 , G01N21/6456
摘要： The invention provides a multiphoton-excitation-type examination apparatus that efficiently generates a multiphoton-excitation effect, that makes the measurement head compact, and that can be easily adjusted when the measurement head is replaced. The multiphoton-excitation-type examination apparatus comprises a laser light source that oscillates ultrashort pulsed laser light; an optical fiber that transmits the ultrashort pulsed laser light from the laser light source; a support member; a measurement head supported on the support member so as to be movable upwards and downwards and at an angle, and having an optical system that irradiates a specimen with the ultrashort pulsed laser light transmitted by the optical fiber that measures fluorescence or reflected light coming from the specimen; and a dispersion-compensating member, in the measurement head, that compensates for group velocity dispersion of the ultrashort pulsed laser light irradiated onto the specimen.
摘要翻译： 本发明提供了一种多光子激发型检查装置，其有效地产生使得测量头紧凑，并且在更换测量头时可以容易地调节的多光子激发效果。 多光子激发型检查装置包括：振荡超短脉冲激光的激光光源; 从激光光源发射超短脉冲激光的光纤; 支撑构件; 测量头支撑在支撑构件上，以便可上下移动一定角度，并且具有光学系统，该光学系统用由光纤透射的超短脉冲激光照射样本，该光纤测量荧光或来自 标本; 以及在测量头中补偿照射到样本上的超短脉冲激光的组速度色散的色散补偿部件。
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公开(公告)号：US20050179903A1
公开(公告)日：2005-08-18
申请号：US11050883
申请日：2005-02-04
CPC分类号： G01N21/648 , G01N21/6458 , G02B21/0088 , G02B21/16
摘要： A total internal reflection fluorescence microscope includes a light source which generates laser light having a plurality of wavelengths, a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination, a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination, an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen, and a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched.
摘要翻译： 全内反射荧光显微镜包括产生具有多个波长的激光的光源，通过物镜以预定的入射角将来自光源的激光照射在样本上并产生ev逝照明的聚焦光学系统 观察由于ev逝照明而产生的荧光的荧光观察单元，调整照射在样本上的激光的入射角的入射角度调节器，以及控制入射角度调节器的控制器， 当切换来自激光光源的激光的波长时，渐逝光的透过深度相同。
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