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    METHOD OF EXTRACTION OF AN ENZYME FROM PLANT OR ANIMAL TISSUE
    51.
    发明申请
    METHOD OF EXTRACTION OF AN ENZYME FROM PLANT OR ANIMAL TISSUE 有权
    从植物或动物组织中提取酶的方法

    公开(公告)号：US20140234871A1

    公开(公告)日：2014-08-21

    申请号：US13768164

    申请日：2013-02-15

    申请人： SYNGENTA PARTICIPATIONS AG

    发明人： Mahathelge Dilip Dias

    IPC分类号： C12N9/24

    CPC分类号： C12N9/2422 ,  C12Y302/01001

    摘要： This invention provides a method for the extraction and detection of a peptide from transgenic plant tissues wherein a non-immunogenic solubility-promoting compound is used to release the enzyme into the solution fraction during the purification process. In some embodiments, this invention provides a method for the extraction and detection of the enzyme Amy797E, which is a heterologous thermo-tolerant α-amylase, from the tissues of corn event 3272 using a non-immunogenic amylase during the purification process. This invention allows for a limit of detection of 1:1000 of Amy797E in an enzyme-linked immunosorbent assay (ELISA).

    摘要翻译： 本发明提供了一种从转基因植物组织提取和检测肽的方法，其中使用非免疫原性溶解度促进化合物在纯化过程中将酶释放到溶液级分中。 在一些实施方案中，本发明提供了在纯化过程中使用非免疫原性淀粉酶从玉米事件3272的组织中提取和检测作为异源耐热α-淀粉酶的酶Amy797E的方法。 本发明允许在酶联免疫吸附测定（ELISA）中检测1×1000的Amy797E的检测限。

    Corn event 3272 and methods of detection thereof
    53.
    发明授权
    Corn event 3272 and methods of detection thereof 有权
    玉米事件3272及其检测方法

    公开(公告)号：US08093453B2

    公开(公告)日：2012-01-10

    申请号：US12608017

    申请日：2009-10-29

    申请人： Brian Johnson ,  Tanya Markham ,  Vladimir Samoylov ,  Kenneth Dallmier

    发明人： Brian Johnson ,  Tanya Markham ,  Vladimir Samoylov ,  Kenneth Dallmier

    IPC分类号： A01H5/00 ,  A01H4/00 ,  C12N15/00 ,  C12N15/05

    CPC分类号： C12N9/2411 ,  A01H5/10 ,  C12N9/2414 ,  C12N9/2422 ,  C12N9/90 ,  C12N15/8242 ,  C12N15/8245 ,  C12Q1/6895 ,  C12Q2600/156

    摘要： A novel transgenic corn event designated 3272, is disclosed. The invention relates to DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the 3272 event and of genomic sequences flanking the insertion sites as well as to assays for detecting the presence of the 3272 event based on these novel sequences. The invention further relates to seeds of corn plants comprising the 3272 genotype, to corn plants comprising the genotype of 3272 and to methods for producing a corn plant by crossing a corn plant comprising the 3272 genotype with itself or another corn variety.

    摘要翻译： 公开了一种名为3272的新型转基因玉米事件。 本发明涉及插入到导致3272事件的玉米基因组中的重组构建体和插入位点侧翼的基因组序列的DNA序列以及基于这些新序列检测3272事件的存在的测定法。 本发明还涉及包含3272基因型的玉米植物的种子，包含3272的基因型的玉米植物，以及通过将包含3272基因型的玉米植物与其本身或另一种玉米品种杂交的玉米植物的生产方法。

    Identification and characterization of a novel alpha-amylase from maize endosperm
    57.
    发明申请
    Identification and characterization of a novel alpha-amylase from maize endosperm 失效
    来自玉米胚乳的新型α-淀粉酶的鉴定和表征

    公开(公告)号：US20080134363A1

    公开(公告)日：2008-06-05

    申请号：US11891589

    申请日：2007-08-10

    申请人： Martha G. James ,  Alan M. Myers ,  Christophe Colleoni ,  Kevin D. Stokes

    发明人： Martha G. James ,  Alan M. Myers ,  Christophe Colleoni ,  Kevin D. Stokes

    IPC分类号： A01H5/00 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06

    CPC分类号： C12N9/2422 ,  C12N15/8245 ,  C12P19/14

    摘要： SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.

    摘要翻译： SHE，在玉米胚乳（Zea mays）中活性的淀粉水解酶，以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而，SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻，拟南芥，苹果和马铃薯中的同源等同物可用于淀粉加工中，用于产生不同的，例如较大尺寸的工业用α限制糊精，与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外，在转基因玉米植物或其他转基因生物（包括细菌，酵母和其他植物物种）中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。

    DNA sequences capable of expressing foreign proteins and metabolites in dicotyledonous plants and cell culture
    60.
    发明授权
    DNA sequences capable of expressing foreign proteins and metabolites in dicotyledonous plants and cell culture 失效
    能够在双子叶植物和细胞培养物中表达外源蛋白质和代谢物的DNA序列

    公开(公告)号：US07045681B2

    公开(公告)日：2006-05-16

    申请号：US10274748

    申请日：2002-10-18

    申请人： Raymond L. Rodriguez ,  Bruce R. Thomas

    发明人： Raymond L. Rodriguez ,  Bruce R. Thomas

    IPC分类号： C12N15/82 ,  C12N15/90 ,  C12N15/29

    CPC分类号： C12N9/2422 ,  C12N15/8234

    摘要： The present invention relates to a method for producing a gene product by expressing a gene encoding said gene product in angiosperm host cells, which method comprises: a) constructing a vector expressible in angiosperm host cells, said vector comprising a promoter region derived from an amylase gene selected from SBAmyA, SBAmyB, SBAmyC genes or SBAmyD of the sugar beet and a gene encoding a desired gene product; b) transforming a compatible angiosperm host cell with said vector; c) cultivating the resulting transformant host cell to a sugar-depleted or sugar-free condition to promote the expression of said gene under the control of such promoter region; and d) recovering the product of the expressed gene. The sugar beet gene when incorporated into a dicot seed or plant has improved biological properties. The gene sequences and the products thereof are claimed.

    摘要翻译： 本发明涉及通过在被子植物宿主细胞中表达编码所述基因产物的基因来生产基因产物的方法，该方法包括：a）构建在被子植物宿主细胞中可表达的载体，所述载体包含衍生自淀粉酶的启动子区 选自SBAmyA，SBAmyB，SBAmyC基因或甜菜的SBAmyD的基因和编码所需基因产物的基因; b）用所述载体转化相容的被子植物宿主细胞; c）将所得的转化体宿主细胞培养至无糖或无糖的条件，以促进所述基因在该启动子区的控制下的表达; 和d）回收表达基因的产物。 掺入双子叶植物种子或植物时的甜菜基因具有改善的生物学性质。 要求保护基因序列及其产物。