公开(公告)号：US20220145342A1

公开(公告)日：2022-05-12

申请号：US17434967

申请日：2020-02-28

申请人： Chr Hansen HMO GmbH

发明人： Stefan JENNEWEIN ,  Dirk WARTENBERG

IPC分类号： C12P19/02 ,  C12P19/04 ,  C12P19/18

摘要： Disclosed are genetically engineered microbial cells for the production of a carbohydrate of interest, wherein the microbial cells possess an increased intracellular availability of at least one sugar phosphate, and produce the carbohydrate of interest when being cultivated in a culture medium containing a mixed monosaccharide feedstock as main carbon and energy source. Also disclosed is a fermentative method of producing a carbohydrate of interest by cultivating said genetically engineered microbial cell in the presence of a mixed monosaccharide feedstock as main carbon and energy source.

公开(公告)号：US11326194B2

公开(公告)日：2022-05-10

申请号：US17091763

申请日：2020-11-06

申请人： DAESANG CORPORATION

发明人： Kyoung Hee Cho ,  Ki Chan Kim ,  Kyoung Ok Park ,  Hak Jun Kim

IPC分类号： C12P19/02 ,  C12P19/22 ,  C13K1/08 ,  C13K7/00

摘要： The present invention provides a method for producing dietary fiber that has a mild sweet taste by reducing sugars and provides by-products having high added values, and dietary fiber produced by the method. The dietary fiber may be produced by including: liquefying roasted dextrin; and adding enzymes including α-amylase, β-amylase, and maltogenic amylase to the liquefied roasted dextrin, and saccharifying the same.

公开(公告)号：US11319560B2

公开(公告)日：2022-05-03

申请号：US17023501

申请日：2020-09-17

申请人： DSM IP ASSETS B.V.

发明人： Johannes Petrus Smits ,  Elisabeth Maria Gierveld

IPC分类号： C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C13K13/00 ,  C13K1/02 ,  C02F3/28 ,  C02F3/34 ,  C12P7/14

摘要： The invention relates to a process for the preparation of a sugar and/or fermentation product from lignocellulosic material.

公开(公告)号：US20220127653A1

公开(公告)日：2022-04-28

申请号：US17429104

申请日：2020-02-11

申请人： BONUMOSE, INC.

发明人： Daniel Joseph WICHELECKI

IPC分类号： C12P19/02 ,  A23L33/125

摘要： Disclosed herein are improved processes for making mannose including the step of converting the M6P to mannose, catalyzed by a M6PP, using enzymes with higher activities compared to M6PPs previously used in a process to produce mannose.

公开(公告)号：US11306300B2

公开(公告)日：2022-04-19

申请号：US16607768

申请日：2018-05-10

申请人： Kansai Chemical Engineering Co., Ltd. ,  Bio-energy Corporation

发明人： Hideo Noda ,  Shinji Hama

IPC分类号： C12N9/40 ,  C12N15/81 ,  C12P7/06 ,  C12P7/56 ,  C12P19/02 ,  C12P19/14

摘要： The present invention provides a transformed microorganism capable of displaying α-galactosidase on its surface layer. Also provided is a method for producing an alcohol, which includes the step of culturing the transformed microorganism in a culture medium containing a material that contains an oligosaccharide α-1,6 linked α-galactose. Also provided is a method for producing lactic acid using such a transformed microorganism together with a material that contains an oligosaccharide α-1,6 linked α-galactose. According to the present invention, a microorganism can be provided, which can degrade an oligosaccharide containing α-1,6 linked α-galactose, which may occur in soybean molasses.

公开(公告)号：US20220112532A1

公开(公告)日：2022-04-14

申请号：US17263401

申请日：2019-07-26

申请人： Global Bioenergies

发明人： Alfredo Alarcon Yanez ,  Samia Boudah ,  Romain Chayot

IPC分类号： C12P19/02 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90

摘要： Described is a method for the production of fructose-6-phosphate (F6P) from dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P) comprising the steps of: (a) enzymatically converting dihydroxyacetone phosphate (DHAP) into dihydroxyacetone (DHA); and (b) enzymatically converting the thus produced dihydroxyacetone (DHA) and glyceraldehyde-3-phosphate (G3P) into fructose-6-phosphate (F6P); or comprising the steps of: (a′) enzymatically converting glyceraldehyde-3-phosphate (G3P) into glyceraldehyde; and (b′) enzymatically converting the thus produced glyceraldehyde together with dihydroxyacetone phosphate (DHAP) into fructose-1-phosphate (F1P); and (c′) enzymatically converting the thus produced fructose-1-phosphate (F1P) into fructose-6-phosphate (F6P).

公开(公告)号：US20220090156A1

公开(公告)日：2022-03-24

申请号：US17465382

申请日：2021-09-02

申请人： Sweetwater Energy, Inc.

发明人： Sarad Parekh ,  Carl P. Felice

IPC分类号： C12P19/14 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00 ,  C12P7/10

摘要： Provided are methods and compositions for high yields while using reduced enzyme loads in saccharification and fermentation processes. These methods increase the efficiency of enzymes and result in improved yields and composition of saccharification and fermentation end products.

公开(公告)号：US11279921B2

公开(公告)日：2022-03-22

申请号：US16603639

申请日：2018-04-10

申请人： NOVOZYMES A/S

发明人： Tomoko Namoto ,  Noriko Tsutsumi ,  Keiichi Ayabe ,  Zhengfang Kang

IPC分类号： C12N9/34 ,  C12P19/02 ,  C12P19/14

摘要： The present invention relates to glucoamylase variants having an increase in raw starch activity compared to the guy-coamylase disclosed as SEQ ID NO: 2, comprising one or more modifications in the catalytic domain and/or one or more modifications in the starch binding domain selected from: a) at least one, preferably at least two, preferably at least three, preferably at least four of: V18M, T43K, N112L, T116R, A117Q, G120S, A271F, Y295W, Q318Y; and/or b) Introducing at least three, preferably at least four substitutions selected from the group: S458C, S458SCGG, S458SGGC, A493V, A518K, E520Q, N527M, S540K, R, S(G)546P, T(V)549W, N503R, N539R+I541Y+T543V+A545S+S546GCGV+G547S+S548T+T549A. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US11274325B2

公开(公告)日：2022-03-15

申请号：US16221193

申请日：2018-12-14

申请人： Glycosyn LLC

发明人： Srikrishnan Mallipeddi ,  Matthew Ian Heidtman ,  Massimo Merighi ,  John M. McCoy

IPC分类号： C12P19/02 ,  C12N15/00 ,  C12N9/24 ,  C12N9/40 ,  C12N9/10 ,  C07K14/245 ,  C12N9/38 ,  C12N9/12

摘要： Provided herein, inter alia, are methods, bacteria, nucleic acids, and polypeptides for producing sialylated oligosaccharides.

公开(公告)号：US11268114B2

公开(公告)日：2022-03-08

申请号：US16467129

申请日：2017-11-24

申请人： Korea University Research and Business Foundation

发明人： Kyoung Heon Kim ,  Sang-Hyun Lee ,  Dong-Hyun Kim ,  Eun-Ju Yun

IPC分类号： C12P19/14 ,  C07H3/04 ,  C12P19/02

摘要： The present invention relates to a method for producing anhydrous galactose derived from seaweed, and provides an improved production yield rate of 3,6-anhydro-L-galactose by using microorganisms during acid treatment, neutralization, and purification after enzymatic hydrolysis, of agarose or agar.