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145 条记录 (耗时 0.047 秒)

    Insecticidal bacteria, and methods for making and using them
    51.
    发明授权
    Insecticidal bacteria, and methods for making and using them 失效
    杀虫细菌，以及制造和使用它们的方法

    公开(公告)号：US06720167B1

    公开(公告)日：2004-04-13

    申请号：US09639576

    申请日：2000-08-14

    申请人： Brian A. Federici ,  Dennis K. Bideshi ,  Hyun-Woo Park ,  Margaret C. Wirth

    发明人： Brian A. Federici ,  Dennis K. Bideshi ,  Hyun-Woo Park ,  Margaret C. Wirth

    IPC分类号： C12P2102

    CPC分类号： A01N63/00 ,  A01N63/02

    摘要： The invention relates to the discovery that nucleic acid sequences comprising a BtI or BtII promoter, or a combination of a BtI and a BtII promoter, a bacterial STAB-SD sequence, and a sequence encoding proteins of the B. sphaericus (“Bs”) binary toxin and expressed in B. thuringiensis (“Bt”) or Bs cells results in production of Bs binary toxin at least 10 times that of untransformed Bs cells. The invention provides nucleic acid sequences, expression vectors, host cells, and methods of increasing the toxicity of an insecticidal bacterium by transforming the bacterium with a nucleic acid sequence of the invention. Further, the invention relates to the discovery that the Cyt1Aa1 protein of Bt subspecies israelensis (“Bti”) reverses resistance to Bs binary toxin in Bs-resistant mosquitoes. The invention provides Bs cells expressing Bti Cyt1Aa1 protein, and methods of reversing resistance to Bs binary toxin by co-administering the Cyt1Aa1 protein with Bs binary toxin.

    摘要翻译： 本发明涉及包含BtI或BtII启动子或BtI和BtII启动子的组合，细菌STAB-SD序列以及编码球形芽孢杆菌（“Bs”）的蛋白质的序列的核酸序列的发现。 二元毒素在苏云金芽孢杆菌（Bt）或Bs细胞中表达，导致Bs二元毒素的产生至少是未转化的Bs细胞的10倍。 本发明通过用本发明的核酸序列转化细菌来提供核酸序列，表达载体，宿主细胞和增加杀虫细菌的毒性的方法。 此外，本发明涉及以下发现：Bt亚种的以色列的Cyt1Aa1蛋白（“Bti”）逆转耐Bs抗性蚊子中Bs二元毒素的抗性。 本发明提供了表达Bti Cyt1Aa1蛋白的Bs细胞，以及通过将Cyt1Aa1蛋白与Bs二元毒素共同施用来逆转Bs二元毒素的抗性的方法。

    Polynucleotides encoding neutrokine-&agr;
    54.
    发明授权
    Polynucleotides encoding neutrokine-&agr; 失效
    编码中性粒细胞α的多核苷酸

    公开(公告)号：US06689579B1

    公开(公告)日：2004-02-10

    申请号：US09005874

    申请日：1998-01-12

    申请人： Guo-Liang Yu ,  Reinhard Ebner ,  Jian Ni

    发明人： Guo-Liang Yu ,  Reinhard Ebner ,  Jian Ni

    IPC分类号： C12P2102

    CPC分类号： C07H21/04 ,  A61K38/00 ,  C07K14/52

    摘要： The present invention relates to a novel Neutrokine-&agr; protein which is a member of the TNF protein family. In particular, isolated nucleic acid molecules are provided encoding the human Neutrokine-&agr; protein including soluble forms of the extracellular domain. Neutrokine-&agr; polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of Neutrokine-&agr; activity. Also provided are diagnostic methods for detecting immune system-related disorders and therapeutic methods for treating immune system-related disorders.

    摘要翻译： 本发明涉及作为TNF蛋白家族成员的新型中枢神经素α蛋白。 特别地，提供编码人Neutrokine-α蛋白的分离的核酸分子，包括细胞外结构域的可溶形式。 还提供了中和型α多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定中枢神经素-α活性的激动剂和拮抗剂的筛选方法。 还提供了用于检测免疫系统相关疾病的诊断方法和用于治疗免疫系统相关疾病的治疗方法。

    Mutant E. coli strains, and their use for producing recombinant polypeptides
    55.
    发明授权
    Mutant E. coli strains, and their use for producing recombinant polypeptides 有权
    突变大肠杆菌菌株及其用于产生重组多肽的用途

    公开(公告)号：US06632639B1

    公开(公告)日：2003-10-14

    申请号：US09762481

    申请日：2001-05-17

    申请人： Marc Dreyfus ,  Pascal Lopez

    发明人： Marc Dreyfus ,  Pascal Lopez

    IPC分类号： C12P2102

    CPC分类号： C12N9/22 ,  C12N15/70

    摘要： The invention concerns the use of Escherichia coli (E. coli) strains whereof the gene coding for the Rnase E comprises a mutation such that the enzyme produced when said mutated gene is expressed no longer has a degrading activity on mRNA, said mutation more significantly not affecting the growth of E. coli strains, for implementing a method for producing specific exogenous recombinant polypeptides.

    摘要翻译： 本发明涉及使用编码Rnase E的基因包含一个或多个氨基酸序列的大肠杆菌（Escherichia coli），大肠杆菌（Escherichia coli），大肠杆菌（Escherichia coli） 突变，使得当所述突变基因被表达时产生的酶不再具有对mRNA的降解活性，所述突变更显着地不影响 E的生长。 大肠杆菌 菌株，用于实施用于产生特异性外源重组多肽的方法。

    Factor IX antihemophilic factor with increased clotting activity
    56.
    发明授权
    Factor IX antihemophilic factor with increased clotting activity 失效
    因子IX抗血友病因子具有增加的凝血活性

    公开(公告)号：US06531298B2

    公开(公告)日：2003-03-11

    申请号：US09118748

    申请日：1998-07-17

    申请人： Darrel W. Stafford ,  Jin Li Chang

    发明人： Darrel W. Stafford ,  Jin Li Chang

    IPC分类号： C12P2102

    CPC分类号： C12N9/644 ,  A61K38/00 ,  C12Y304/21022

    摘要： A non-naturally occuring Factor IX protein having an amino acid substitution at amino acid position 338 is provided. Preferred substitutions include the substitution of analanine, leucine, or valine for the arginine at amino acid position 338. Factor IX of the present invention is non-naturally occuring (e.g., does not contain only an arginine to proline substitution at amino acid position 338). Factor IX proteins of the invention are useful for facilitating blood clotting in subjects in need thereof, such as subjects afflicted with hemophilia B. Pharmaceutical formulations comprising Factor IX of the invention are provided, along with nucleic acids encoding the factor and vectors containing such nucleic acids.

    摘要翻译： 提供了在氨基酸位置338具有氨基酸取代的非天然存在的因子IX蛋白质。 优选的取代包括在氨基酸位置338将精氨酸替换为鸟嘌呤，亮氨酸或缬氨酸。本发明的因子IX是非天然存在的（例如，在氨基酸位置338处不含有精氨酸至脯氨酸取代） 。 本发明的因子IX蛋白质可用于促进有需要的受试者例如患有血友病B的受试者的血液凝固。提供包含本发明的因子IX的药物制剂以及编码所述因子的核酸和含有该核酸的载体 。

    Cloning and recombinant production of CRF receptor(s)
    59.
    发明授权
    Cloning and recombinant production of CRF receptor(s) 失效
    CRF受体的克隆和重组产生

    公开(公告)号：US06495343B1

    公开(公告)日：2002-12-17

    申请号：US08374009

    申请日：1995-01-17

    申请人： Marilyn H. Perrin ,  Ruoping Chen ,  Kathy A. Lewis ,  Wylie W. Vale, Jr. ,  Cynthia J. Donaldson ,  Paul Sawchenko

    发明人： Marilyn H. Perrin ,  Ruoping Chen ,  Kathy A. Lewis ,  Wylie W. Vale, Jr. ,  Cynthia J. Donaldson ,  Paul Sawchenko

    IPC分类号： C12P2102

    CPC分类号： C07K14/723 ,  A61K38/00 ,  C07K14/72

    摘要： In accordance with the present invention, there are provided novel G-protein-coupled receptor proteins (CRF-R) characterized by having sufficient binding affinity for corticotropin releasing factor (CRF) such that concentrations of ≦10 nM of CRF occupy ≧50% of the binding sites of said receptor protein. Nucleic acid sequences encoding such receptors, assays employing same, as well as antibodies derived therefrom, are also disclosed. Invention CRF-Rs can be employed in a variety of ways, such as, for example, in bioassays, for production of antibodies thereto, in therapeutic compositions containing such proteins and/or antibodies.

    摘要翻译： 根据本发明，提供了新颖的G蛋白偶联受体蛋白（CRF-R），其特征在于对促皮质激素释放因子（CRF）具有足够的结合亲和力，使得CRF的<= 10nM的浓度占据≥50 所述受体蛋白的结合位点的百分比。 还公开了编码这种受体的核酸序列，使用它们的测定法，以及由其衍生的抗体。 本发明CRF-Rs可以以各种方式使用，例如在生物测定中用于生产抗体，在含有这种蛋白质和/或抗体的治疗组合物中。

    Apoptosis inducing molecule II
    60.
    发明授权
    Apoptosis inducing molecule II 失效
    凋亡诱导分子II

    公开(公告)号：US06479254B2

    公开(公告)日：2002-11-12

    申请号：US09027287

    申请日：1998-02-20

    申请人： Reinhard Ebner ,  Guo-Liang Yu ,  Steven M. Ruben

    发明人： Reinhard Ebner ,  Guo-Liang Yu ,  Steven M. Ruben

    IPC分类号： C12P2102

    CPC分类号： C07K14/70575 ,  A61K38/00 ,  C07K2319/00 ,  C12N2799/026 ,  Y02A50/411 ,  Y10S930/144

    摘要： The present invention relates to a novel member of the TNF-Ligand superfamily, Apoptosis Inducing Molecule II (AIM II). In particular, isolated nucleic acid molecules are provided encoding the human AIM II protein. AIM II polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of AIM II activity. Also provided are therapeutic methods for treating lymphadenopathy, autoimmune disease, graft versus host disease, and to inhibit neoplasia, such as tumor cell growth.

    摘要翻译： 本发明涉及TNF-配体超家族，细胞凋亡诱导分子II（AIM II）的新成员。 特别地，提供编码人AIM II蛋白质的分离的核酸分子。 还提供AIM II多肽，载体，宿主细胞以及用于制备其的重组方法。 本发明还涉及用于鉴定AIM II活性的激动剂和拮抗剂的筛选方法。 还提供了用于治疗淋巴结病，自身免疫疾病，移植物抗宿主病和抑制肿瘤形成如肿瘤细胞生长的治疗方法。