公开(公告)号：US20070238646A1

公开(公告)日：2007-10-11

申请号：US11765494

申请日：2007-06-20

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

IPC分类号： A61K38/00 ,  C07K1/00

CPC分类号： C07H21/04 ,  C07K14/325

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列的分离的核酸分子及其变体

公开(公告)号：US20070136840A1

公开(公告)日：2007-06-14

申请号：US11605824

申请日：2006-11-29

申请人： Cheryl Peters ,  Jill Hinson ,  Philip Hammer ,  Brian Vande Berg ,  Laura Schouten ,  Brian Carr

发明人： Cheryl Peters ,  Jill Hinson ,  Philip Hammer ,  Brian Vande Berg ,  Laura Schouten ,  Brian Carr

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8275 ,  C07K14/195 ,  C07K14/415 ,  C12N15/8274 ,  C12Q1/42

摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.

摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。 本发明另外提供了使用荧光底物测量酶动力学活性的方法。

公开(公告)号：US20070044178A1

公开(公告)日：2007-02-22

申请号：US11416261

申请日：2006-05-02

申请人： Nadine Carozzi ,  Michael Koziel ,  Tracy Hargiss ,  Nicholas Duck ,  Theodore Kahn

发明人： Nadine Carozzi ,  Michael Koziel ,  Tracy Hargiss ,  Nicholas Duck ,  Theodore Kahn

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04 ,  A01N65/00 ,  C07K14/415 ,  A01H1/00

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,15,17或19所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 3,14,16或18，以及其变体和片段。

公开(公告)号：US20040250311A1

公开(公告)日：2004-12-09

申请号：US10781979

申请日：2004-02-19

申请人： Athenix Corporation

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck ,  Brian Carr

IPC分类号： C12N015/82 ,  C07H021/04 ,  C12N015/87 ,  C12N015/09 ,  C07K014/00 ,  A01H005/10 ,  C12N005/10 ,  C12N015/63 ,  A01H005/00 ,  A61K039/395

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3, 5, and 7, and the nucleotide sequences set forth in SEQ ID NO:1, 2, 4, and 6, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素和δ-内毒素相关多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素和δ-内毒素相关的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,5和7所示氨基酸序列的核苷酸序列，以及SEQ ID NO： 和6，以及其变体和片段。

公开(公告)号：US20040231060A1

公开(公告)日：2004-11-25

申请号：US10795102

申请日：2004-03-05

申请人： Athenix Corporation

发明人： Jill Burdette ,  Brian Vande Berg ,  Brian Carr ,  Nicholas B. Duck ,  Michael G. Koziel ,  Nadine Carozzi ,  Paresma R. Patel

IPC分类号： D06M010/00

CPC分类号： C12P7/10 ,  C08H8/00 ,  C12N9/0006 ,  C12N9/18 ,  C12N9/20 ,  D21C5/005 ,  D21C9/16 ,  D21C11/0007 ,  Y02E50/16

摘要： Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.

摘要翻译： 提供水解木质纤维素的方法，包括使木质纤维素与至少一种化学处理接触。 还提供了用于预处理木质纤维素材料的方法，包括使材料与至少一种化学品接触。 还提供了用于从植物材料释放诸如酶，药物或营养品的物质的方法。 这些方法比目前的方法更有效，更经济，毒性更低。

公开(公告)号：US20040205847A1

公开(公告)日：2004-10-14

申请号：US10796953

申请日：2004-03-10

申请人： Athenix Corporation

发明人： Philip E. Hammer ,  Todd K. Hinson ,  Brian Carr ,  Nicholas B. Duck

IPC分类号： A01H001/00 ,  C12N015/82 ,  C07H021/04 ,  C07K014/435

CPC分类号： C12N15/8275 ,  C12N9/88

摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding an amino acid sequence shown in SEQ ID NO:3, 6, 8, 11, 19, or 21, or a nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 5, 7, 9, 10, 18, or 20, as well as variants and fragments thereof.

摘要翻译： 提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。 提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的植物，植物细胞，组织和种子。 特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,6,8,11,19或21所示氨基酸序列的核苷酸序列或SEQ ID NO： 1，2，4，5，7，9，10，18或20，以及其变体和片段。

公开(公告)号：US5119494A

公开(公告)日：1992-06-02

申请号：US551592

申请日：1990-07-10

申请人： Jonathan D. Garman

发明人： Jonathan D. Garman

IPC分类号： G06F3/14 ,  G09G5/14 ,  G09G5/36 ,  G09G5/39 ,  G09G5/393

CPC分类号： G09G5/14 ,  G06F3/1423 ,  G09G5/363 ,  G09G2360/121 ,  G09G5/393

摘要： In accordance with the present invention, an address mapper is provided between the CPU running a MS-DOS application program and a frame buffer, so as to allow the frame buffer to be mapped into the CPU address space expected by a MS-DOS application program. A write operation by the MS-DOS application program into the CPU's display address space is translated by the address mapper to be performed in the address space of the frame buffer, providing to the user the appearance as if the frame buffer is part of the CPU's address space ("Virtual MS-DOS display"). This frame buffer may have an address space larger than that used by the MS-DOS application program for writing to the display. This additional space can be allocated to other processes, which are each protected from inadvertently reading or writing into another process' allocated memory space.

摘要翻译： 根据本发明，在运行MS-DOS应用程序的CPU与帧缓冲器之间提供地址映射器，以便将帧缓冲器映射到MS-DOS应用程序期望的CPU地址空间 。 MS-DOS应用程序对CPU显示地址空间的写操作由地址映射器进行转换，以在帧缓冲区的地址空间中执行，向用户提供外观，就像帧缓冲区是CPU的一部分 地址空间（“虚拟MS-DOS显示”）。 该帧缓冲器可能具有比用于写入显示器的MS-DOS应用程序使用的地址空间更大的地址空间。 这个额外的空间可以被分配给其他进程，每个进程都被保护，以免无意中读取或写入另一个进程的分配的内存空间。

公开(公告)号：US08829279B2

公开(公告)日：2014-09-09

申请号：US13168454

申请日：2011-06-24

申请人： Nadine Carozzi ,  Michael G. Koziel ,  Nicholas Duck ,  Nalini M. Desai ,  Rong Guo ,  Daniel John Tomso ,  Rebekah Deter ,  Tracy Hargiss

发明人： Nadine Carozzi ,  Michael G. Koziel ,  Nicholas Duck ,  Nalini M. Desai ,  Rong Guo ,  Daniel John Tomso ,  Rebekah Deter ,  Tracy Hargiss

IPC分类号： A01H5/00 ,  C12N15/82 ,  C07K14/325 ,  A01N37/18

CPC分类号： C12N15/8286 ,  A01N37/44 ,  C07K14/325 ,  C12P21/02 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。

公开(公告)号：US08563808B2

公开(公告)日：2013-10-22

申请号：US11765491

申请日：2007-06-20

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck ,  Brian Carr

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N1/21 ,  C12N15/32

CPC分类号： C07H21/04 ,  C07K14/325

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.

公开(公告)号：US20130167264A1

公开(公告)日：2013-06-27

申请号：US13779524

申请日：2013-02-27

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso ,  Rong Guo

IPC分类号： C07K14/195 ,  C12N15/82

CPC分类号： C12N15/8286 ,  C07K14/195 ,  C07K14/325 ,  C07K14/345 ,  Y02A40/162

摘要： Compositions and methods for conferring insecticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for a Brevibacillus-derived delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 7, or 10, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 6, 8, 9, 11, 12, 13, 14, or 15, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含短杆菌属来源的δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了具有编码SEQ ID NO：2,4,7或10所示的氨基酸序列的核苷酸序列的分离的核酸分子或者SEQ ID NO： 5，6，8，9，11，12，13，14或15，以及其变体和片段。