公开(公告)号：US20210102263A1

公开(公告)日：2021-04-08

申请号：US17120500

申请日：2020-12-14

Applicant: Mayo Foundation for Medical Education and Research ,  Exact Sciences Development Company, LLC

Inventor： David A. Ahlquist ,  William R. Taylor ,  Douglas W. Mahoney ,  Graham P. Lidgard ,  Hatim T. Allawi

IPC: C12Q1/6886

Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as colorectal cancer.

公开(公告)号：US20200299778A1

公开(公告)日：2020-09-24

申请号：US16782841

申请日：2020-02-05

Applicant: Mayo Foundation for Medical Education and Research ,  Exact Sciences Development Company, LLC

Inventor： David A. Ahlquist ,  William R. Taylor ,  John B. Kisiel ,  Tracy C. Yab ,  Douglas W. Mahoney ,  Hatim T. Allawi

IPC: C12Q1/6886

Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as gastric cancer.

公开(公告)号：US20200248233A1

公开(公告)日：2020-08-06

申请号：US16789279

申请日：2020-02-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Hatim T. Allawi ,  William G. Weisburg ,  Graham P. Lidgard ,  Michael W. Kaiser ,  Abram M. Vaccaro ,  Gracie Shea

IPC: C12Q1/686 ,  C12Q1/6827 ,  C12N15/65

Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

公开(公告)号：US20200216913A1

公开(公告)日：2020-07-09

申请号：US16810385

申请日：2020-03-05

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim T. Allawi ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12Q1/6881

Abstract: Provided herein is technology relating compositions and methods for detecting tissue cell-specific DNA, such as epithelial cell-specific DNA, in blood or blood products from a subject. The technology also relates to use of tissue cell-specific DNAs as internal controls for methylation assays.

公开(公告)号：US10704083B2

公开(公告)日：2020-07-07

申请号：US16164373

申请日：2018-10-18

Applicant: Exact Sciences Development Company, LLC

Inventor： Michael J. Domanico ,  Hatim Allawi ,  Graham P. Lidgard ,  Brian Aizenstein ,  Oliver Hunt ,  Tobias Charles Zutz

IPC: C12Q1/6806 ,  C12Q1/6851

Abstract: Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.

公开(公告)号：US20200040377A1

公开(公告)日：2020-02-06

申请号：US16318578

申请日：2017-07-19

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim T. Allawi ,  Graham P. Lidgard ,  Brian Aizenstein ,  Melissa M. Gray

IPC: C12Q1/6806 ,  C12N15/10

Abstract: The present invention provides synthetic DNA strands that find use as process controls in DNA processing and nucleic acid testing methods. In particular, provided herein are synthetic methylated DNA strands of known composition for use as control molecules in DNA testing, e.g., of mutations and/or methylation of DNA isolated from non-fish samples, such as human samples.

公开(公告)号：US20190276900A1

公开(公告)日：2019-09-12

申请号：US16421407

申请日：2019-05-23

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Joost Louwagie

IPC: C12Q1/6886 ,  G01N33/574

Abstract: A method of processing a fecal sample from a human subject comprising removing a portion of a collected fecal sample and adding the removed portion of the sample to a buffer that prevents denaturation or degradation of blood proteins found in the sample, and detecting the presence of human blood in the removed portion of the fecal sample. The method further comprises stabilizing the remaining portion of the fecal sample.

公开(公告)号：US20180346993A1

公开(公告)日：2018-12-06

申请号：US15987793

申请日：2018-05-23

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/6886 ,  C12Q1/6881 ,  C12Q1/6858

CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2525/301 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3′ terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3′ terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.

公开(公告)号：US10138524B2

公开(公告)日：2018-11-27

申请号：US15105178

申请日：2014-12-19

Applicant: Exact Sciences Development Company, LLC

Inventor： Ilse A. Tyler ,  Keith Yaeger ,  Michael J. Domanico ,  Hatim Allawi ,  Graham P. Lidgard

IPC: C12N15/11 ,  C12Q1/6886 ,  C12Q1/6876 ,  C12Q1/68

Abstract: The present invention provides synthetic DNA strands that find use as controls or in nucleic acid testing methods. In particular, provided herein are synthetic DNA strands of known composition for use as control molecules in stool DNA testing, e.g., of mutations and/or methylation of DNA isolated from stool samples.

公开(公告)号：US10106844B2

公开(公告)日：2018-10-23

申请号：US14939651

申请日：2015-11-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6851

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.