公开(公告)号：US20120315661A1

公开(公告)日：2012-12-13

申请号：US13471047

申请日：2012-05-14

Applicant: Zhenjun DIWU ,  Jixiang LIU ,  Kyle GEE

Inventor： Zhenjun DIWU ,  Jixiang LIU ,  Kyle GEE

IPC: G01N21/75 ,  C07D491/147 ,  G01N21/64 ,  C07D495/04 ,  C07D495/14 ,  C07D498/14 ,  C07D495/22 ,  C07D491/22

CPC classification number: G01N33/582 ,  C07D471/04 ,  C07D491/04 ,  C07D491/044 ,  C07D491/14 ,  C07D491/147 ,  C07D491/22 ,  C07D495/04 ,  C07D495/14 ,  C07D495/22 ,  C07D498/14 ,  C12Q1/6816 ,  Y10S436/80 ,  Y10T436/141111 ,  Y10T436/143333 ,  Y10T436/145555 ,  C12Q2563/107

Abstract: The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.

Abstract translation: 本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。 本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。 本发明的许多染料是有用的荧光染料。 本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其他分析物中的用途。

公开(公告)号：US08329390B2

公开(公告)日：2012-12-11

申请号：US11212203

申请日：2005-08-25

Applicant: Zhenjun Diwu ,  Jianjun He ,  Yi Tang

Inventor： Zhenjun Diwu ,  Jianjun He ,  Yi Tang

IPC: C12Q1/00 ,  C07D403/00 ,  C07D239/10 ,  C07D239/66

CPC classification number: C07D239/66 ,  G01N33/52

Abstract: The present invention relates generally to the detection and measurement of transmembrane potentials using an N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonol (shown below). In particular, the present invention is directed to compositions and optical methods for determining transmembrane potentials across the plasma membrane of biological cells using a slightly hydrophobic N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonols. The method comprises a slightly hydrophobic N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonol anion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes. wherein R1, R2, and R3 are independently selected from the group consisting of hydrogen, alkyl, haloalkyl and heteroalkyl; n is an integer from 1 to 3; Z is Na, K, ammonium or other biologically acceptable salt.

Abstract translation: 本发明一般涉及使用N，N，N'-三烷基硫代巴比妥酸衍生的聚甲基氧杂多酚（如下所示）检测和测量跨膜电位。 特别地，本发明涉及使用稍微疏水的N，N，N'-三烷基硫代巴比妥酸衍生的聚甲基氧杂多糖测定生物细胞质膜跨膜电位的组合物和光学方法。 该方法包括一种稍微疏水的N，N，N'-三烷基硫代巴比妥酸衍生的聚甲基氧杂氮杂阴离子，其能够响应膜的电位变化而从膜的第一面再分配到膜的第二面。 在一个方面，该方法用于鉴定调节生物膜中膜电位的化合物。 其中R1，R2和R3独立地选自氢，烷基，卤代烷基和杂烷基; n为1〜3的整数， Z是Na，K，铵或其他生物可接受的盐。

公开(公告)号：US08318953B2

公开(公告)日：2012-11-27

申请号：US12822091

申请日：2010-06-23

Applicant: Zhenjun Diwu ,  Chunmei Wei ,  Qinglin Meng ,  Haitao Guo ,  Jingfang Liao

Inventor： Zhenjun Diwu ,  Chunmei Wei ,  Qinglin Meng ,  Haitao Guo ,  Jingfang Liao

IPC: C07D405/12

CPC classification number: C12Q1/04 ,  C07D405/12 ,  C07D405/14 ,  C07D409/14 ,  C07D413/14 ,  C07D417/14 ,  G01N33/5005

Abstract: Chemically reactive 7-hydroxycoumarin derivatives and their application for analyzing cell function, for example in combination with additional fluorescent labels. The coumarin derivatives exhibit a strong absorption at 405 nm and high fluorescence quantum yields.

Abstract translation: 化学反应性7-羟基香豆素衍生物及其用于分析细胞功能的应用，例如与额外的荧光标记组合。 香豆素衍生物在405nm处表现出强吸收和高荧光量子产率。

公开(公告)号：US20120258514A1

公开(公告)日：2012-10-11

申请号：US13374994

申请日：2012-01-27

Applicant: Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

Inventor： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

IPC: C12N9/96 ,  C12N1/20 ,  C07H7/06 ,  C07H21/04 ,  C07K14/00 ,  C12N5/071 ,  C07K2/00

CPC classification number: C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了在1位和3'-位之间分子内交联的化学反应性碳菁染料，它们的生物缀合物及其用途。 1,3'-交联的碳氰酸酯优于光谱相似的1,1'-交联或非交联染料的共轭物。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US20120202220A1

公开(公告)日：2012-08-09

申请号：US13236211

申请日：2011-09-19

Applicant: Rajendra SINGH ,  Ning ZHANG ,  Jae Beom KIM ,  Zhenjun DIWU

Inventor： Rajendra SINGH ,  Ning ZHANG ,  Jae Beom KIM ,  Zhenjun DIWU

IPC: G01N33/567 ,  A61B6/00 ,  C07K14/52 ,  C07H1/00 ,  C07K2/00

CPC classification number: A61K49/0021 ,  A61K49/0032 ,  A61K49/0052 ,  A61K49/0056 ,  G01N33/533 ,  G01N33/582 ,  G01N2333/9027 ,  G01N2400/00

Abstract: Multivalent fluorescent probes and methods of using these multivalent fluorescent probes for in vitro and in vivo imaging are described.

Abstract translation: 描述了多价荧光探针和使用这些多价荧光探针用于体外和体内成像的方法。

公开(公告)号：US20120183954A1

公开(公告)日：2012-07-19

申请号：US13181107

申请日：2011-07-12

Applicant: Zhenjun Diwu ,  Qinglin Meng ,  Jinfang Liao ,  Haitao Guo ,  Timothy Dubrovsky ,  Barnaby Abrams

Inventor： Zhenjun Diwu ,  Qinglin Meng ,  Jinfang Liao ,  Haitao Guo ,  Timothy Dubrovsky ,  Barnaby Abrams

IPC: G01N21/64 ,  C07D498/16 ,  C07F5/00 ,  C12N9/96 ,  C07D403/14 ,  C07K17/02 ,  C08B37/02 ,  C07H19/00 ,  C07K5/00 ,  C07D487/14

CPC classification number: C09B23/083 ,  C09B11/08 ,  C09B11/24 ,  C09B11/26 ,  C09B57/00 ,  C09B57/10 ,  G01N33/533

Abstract: Chemically reactive dyes that are intramolecularly crosslinked with a water-soluble bridge, their bioconjugates and their uses are described. Reactive fluorescent dyes that have a water-soluble bridge are superior to those of conjugates of spectrally non-crosslinked dyes or the dyes that are crosslinked with a hydrophobic bridge. The invention includes reactive fluorescent dyes, their biological conjugates and uses.

Abstract translation: 描述了与水溶性桥分子内交联的化学活性染料及其生物共轭物及其用途。 具有水溶性桥梁的活性荧光染料优于光谱非交联染料或与疏水桥交联的染料的共轭物。 本发明包括反应性荧光染料，它们的生物共轭物和用途。

公开(公告)号：US08183375B2

公开(公告)日：2012-05-22

申请号：US12901713

申请日：2010-10-11

Applicant: Zhenjun Diwu ,  Jixiang Liu ,  Kyle Gee

Inventor： Zhenjun Diwu ,  Jixiang Liu ,  Kyle Gee

IPC: C07D471/04 ,  C07D471/06 ,  G01N1/30 ,  G01N33/48 ,  G01N33/50

CPC classification number: G01N33/582 ,  C07D471/04 ,  C07D491/04 ,  C07D491/044 ,  C07D491/14 ,  C07D491/147 ,  C07D491/22 ,  C07D495/04 ,  C07D495/14 ,  C07D495/22 ,  C07D498/14 ,  C12Q1/6816 ,  Y10S436/80 ,  Y10T436/141111 ,  Y10T436/143333 ,  Y10T436/145555 ,  C12Q2563/107

Abstract: The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.

Abstract translation: 本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。 本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。 本发明的许多染料是有用的荧光染料。 本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其他分析物中的用途。

公开(公告)号：US08093404B2

公开(公告)日：2012-01-10

申请号：US12583513

申请日：2009-08-20

Applicant: Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

Inventor： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

IPC: C07D403/06

CPC classification number: C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了在1位和3'-位之间分子内交联的化学反应性碳菁染料，它们的生物缀合物及其用途。 1,3'-交联的碳氰酸酯优于光谱相似的1,1'-交联或非交联染料的共轭物。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US07985552B2

公开(公告)日：2011-07-26

申请号：US12807268

申请日：2010-09-01

Applicant: Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

Inventor： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang ,  Xiang Guobing

IPC: C12Q1/68 ,  C12Q1/70 ,  G01N33/53 ,  C07D225/00 ,  C07D209/56

CPC classification number: C09B23/06 ,  C07D487/14 ,  C07D487/22 ,  C07D498/22 ,  C09B23/0066 ,  C09B23/02 ,  G01N33/52 ,  G01N33/533 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了在1位和3'-位之间分子内交联的化学反应性碳菁染料，它们的生物缀合物及其用途。 1,3'-交联的碳氰酸酯优于光谱相似的1,1'-交联或非交联染料的共轭物。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US07910753B2

公开(公告)日：2011-03-22

申请号：US11222049

申请日：2005-09-07

Applicant: Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang

Inventor： Zhenjun Diwu ,  Jianheng Zhang ,  Yi Tang

IPC: C07D209/46 ,  C07D209/02

CPC classification number: C07D409/14 ,  C07D209/60 ,  C07D403/14 ,  C07D409/04 ,  C07D409/06 ,  C07D417/14 ,  C07D471/04 ,  C07H21/04 ,  C07J43/00 ,  G01N33/542 ,  G01N33/582

Abstract: The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.

Abstract translation: 本发明的淬灭化合物是被一个或多个杂芳族猝灭部分取代的弱发光花青素。 本发明的淬灭化合物表现出很少或不可观察到的发光，并有效地淬灭广谱的发光化合物。 化学反应猝灭化合物具有用于标记各种物质（包括生物分子）的效用。 这些标记的物质对于各种能量转移测定和应用是非常有用的。