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72 条记录 (耗时 0.034 秒)

    Method of identifying a gene product
    61.
    发明授权
    Method of identifying a gene product 有权
    识别基因产物的方法

    公开(公告)号：US06861229B2

    公开(公告)日：2005-03-01

    申请号：US10336702

    申请日：2003-01-03

    申请人： Miguel E. Carrión ,  Imre Kovesdi

    发明人： Miguel E. Carrión ,  Imre Kovesdi

    IPC分类号： C12N15/10 ,  C12N15/861 ,  C12Q1/68 ,  G01N33/53 ,  C12N15/63 ,  C12N15/70 ,  C11N15/861

    CPC分类号： C12N15/86 ,  C12N15/1055 ,  C12N2710/10343 ,  C12Q1/6816 ,  C12Q1/6897 ,  C12Q2563/167

    摘要： The present invention provides a method of identifying a gene product. The method comprises providing a multiplicity of cells comprising a first gene product. Preferably, the first gene product is produced in the multiplicity of cells by expressing a first exogenous nucleic acid sequence encoding the first gene product. A library of second nucleic acid sequences encoding second gene products is then introduced into the multiplicity of cells. The second nucleic acid sequences are expressed in the multiplicity of cells to produce the second gene products such that the first gene product and at least one of the second gene products contact. The method further comprises causing a complex to form between the first gene product, an affinity molecule that binds the first gene product, and at least one of the second gene products, and subsequently retrieving the complex. At least one second gene product of the complex then is identified.

    摘要翻译： 本发明提供鉴定基因产物的方法。 该方法包括提供包含第一基因产物的多个细胞。 优选地，通过表达编码第一基因产物的第一外源核酸序列，在多种细胞中产生第一基因产物。 然后将编码第二基因产物的第二核酸序列的文库导入多个细胞。 第二核酸序列在多重细胞中表达以产生第二基因产物，使得第一基因产物和第二基因产物中的至少一个接触。 所述方法还包括使第一基因产物，结合第一基因产物的亲和分子与至少一种第二基因产物形成复合物，随后检索该复合物。 然后鉴定该复合物的至少一个第二基因产物。

    Method of modulating neovascularization
    62.
    发明授权
    Method of modulating neovascularization 失效
    调节新血管形成的方法

    公开(公告)号：US06753321B2

    公开(公告)日：2004-06-22

    申请号：US09952498

    申请日：2001-09-14

    申请人： Imre Kovesdi

    发明人： Imre Kovesdi

    IPC分类号： A61K4800

    CPC分类号： A61K38/1866 ,  A61K38/179 ,  A61K38/18 ,  A61K38/1891 ,  A61K38/195 ,  A61K48/00 ,  C12N15/86 ,  C12N2710/10343 ,  A61K2300/00

    摘要： The present invention provides a method of modulating neovascularization in an animal. The method comprises administering to the animal two or more nucleic acid sequences, each nucleic acid sequence encoding at least one angiogenesis-modulation factor that acts upon a different angiogenic process, such that the nucleic acid sequences are expressed to produce the angiogenesis-modulation factors to modulate neovascularization in the animal. Modulating neovascularization includes the induction of neovascularization or, in the alternative, the inhibition or reduction of neovascularization.

    摘要翻译： 本发明提供一种调节动物体内新血管形成的方法。 该方法包括向动物施用两个或更多个核酸序列，每个核酸序列编码至少一种血管生成调节因子，其作用于不同的血管生成过程，使得所述核酸序列被表达以产生血管生成调节因子 调节动物的新生血管形成。 调节新血管形成包括新血管形成的诱导，或者替代地，抑制或减少新生血管形成。

    Use of trans-activation and CIS-activation to increase the persistence of a transgene in an at least E4-deficient adenovirus
    65.
    发明授权
    Use of trans-activation and CIS-activation to increase the persistence of a transgene in an at least E4-deficient adenovirus 失效
    使用反式激活和CIS激活增加至少E4缺陷型腺病毒中转基因的持续性

    公开(公告)号：US06660521B2

    公开(公告)日：2003-12-09

    申请号：US09771832

    申请日：2001-01-29

    申请人： Douglas E. Brough ,  Imre Kovesdi

    发明人： Douglas E. Brough ,  Imre Kovesdi

    IPC分类号： C12N1500

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N15/63 ,  C12N2710/10322 ,  C12N2710/10345 ,  C12N2710/16622 ,  C12N2830/60 ,  C12N2840/60

    摘要： The present invention provides a method of modulating the persistence of expression of a transgene in an at least E4&Dgr; adenoviral vector in a cell. In one embodiment, the method comprises contacting the cell with an at least E4&Dgr; adenoviral vector comprising (i) a transgene and (ii) a gene encoding a trans-acting factor, which is not from the E4 region of an adenovirus and which modulates the persistence of expression of the transgene. In another embodiment, the method comprises contacting the cell simultaneously or sequentially with (i) an at least E4&Dgr; adenoviral vector comprising a transgene and (ii) a viral vector comprising a gene encoding a trans-acting factor, which is not from the E4 region of an adenovirus and which modulates the persistence of expression of the transgene. In addition, the present invention provides a recombinant at least E4&Dgr; adenoviral vector for use in the method and a composition comprising the vector and a carrier therefor. Also provided by the present invention is a system for modulation of a recombinant at least E4&Dgr; adenoviral vector for use in the method and a composition comprising the system and a carrier therefor.

    摘要翻译： 本发明提供调节细胞中至少E4Delta腺病毒载体中转基因表达的持续性的方法。 在一个实施方案中，该方法包括使细胞与至少E4Delta腺病毒载体接触，所述至少E4Delta腺病毒载体包含（i）转基因和（ii）编码反式作用因子的基因，其不是来自腺病毒的E4区域，并且其调节 持续表达的转基因。 在另一个实施方案中，该方法包括将细胞同时或顺序地与（i）包含转基因的至少E4Delta腺病毒载体接触，和（ii）包含编码反作用因子的基因的病毒载体，其不是来自E4区 的腺病毒，并且其调节转基因表达的持续性。 此外，本发明提供了用于该方法的重组至少E4Delta腺病毒载体和包含载体及其载体的组合物。 本发明还提供了一种用于调节用于该方法的重组至少E4Delta腺病毒载体的系统和包含该系统和其载体的组合物。

    Efficient purification of adenovirus
    67.
    发明授权
    Efficient purification of adenovirus 有权

    公开(公告)号：US06586226B2

    公开(公告)日：2003-07-01

    申请号：US09997909

    申请日：2001-11-30

    申请人： Miguel E. Carrión ,  Marilyn Menger ,  Imre Kovesdi

    发明人： Miguel E. Carrión ,  Marilyn Menger ,  Imre Kovesdi

    IPC分类号： C12N702

    CPC分类号： C07K14/005 ,  B01D15/345 ,  B01D15/363 ,  B01J41/20 ,  C12N7/00 ,  C12N2710/10322 ,  C12N2710/10351 ,  C12Q1/06 ,  G01N30/06 ,  G01N30/96 ,  G01N2030/625 ,  G01N2030/8813 ,  G01N2333/075

    摘要： A method of enriching a solution of an adenovirus comprising applying a mixed solution comprising an adenovirus and at least one undesired type of biomolecule to an anion exchange chromatography resin containing a binding moiety selected from the group consisting of dimethylaminopropyl, dimethylaminobutyl, dimethylaminoisobutyl, and dimethylaminopentyl and eluting the adenovirus from the chromatography resin. Also provided is a method of purifying an adenovirus from adenovirus-infected cells comprising lysing such cells, applying the lysate to a single chromatography resin, eluting the adenovirus from the chromatography resin, and collecting a fraction containing adenovirus that is substantially as pure as triple CsCl density gradient-purified adenovirus. The present method further provides a method of accurately quantifying the number of adenoviral particles in a solution of adenovirus comprising applying to and eluting from an anion exchange chromatography resin a sample solution of adenovirus, comparing the absorbance of the sample solution of adenovirus and the absorbance of a standard solution of adenovirus, and quantifying the number of adenoviral particles in the sample solution.

    Polynucleotide encoding chimeric protein and related vector, cell, and method of expression thereof
    68.
    发明授权
    Polynucleotide encoding chimeric protein and related vector, cell, and method of expression thereof 有权
    编码嵌合蛋白和相关载体的多核苷酸，细胞及其表达方法

    公开(公告)号：US06472176B2

    公开(公告)日：2002-10-29

    申请号：US09736743

    申请日：2000-12-14

    申请人： Imre Kovesdi ,  Joseph T. Bruder

    发明人： Imre Kovesdi ,  Joseph T. Bruder

    IPC分类号： C12P2100

    CPC分类号： C12N15/625 ,  A61K47/646 ,  C07K2319/04 ,  C07K2319/09 ,  C07K2319/10 ,  C07K2319/71 ,  C07K2319/75 ,  C07K2319/80

    摘要： The invention pertains to a polynucleotide encoding a chimeric protein comprising an endoplasmic reticulum localization signal peptide, a transport moiety, and a moiety of interest, wherein the endoplasmic reticulum localization signal peptide, the transport moiety, and the moiety of interest are operably linked with each other, a vector comprising the polynucleotide, a cell comprising such a vector, and a method of expressing a protein comprising the transport moiety and the moiety of interest.

    摘要翻译： 本发明涉及编码包含内质网定位信号肽，转运部分和感兴趣部分的嵌合蛋白的多核苷酸，其中内质网定位信号肽，转运部分和感兴趣的部分与每个 另一方面，包含多核苷酸的载体，包含这种载体的细胞，以及表达包含转运部分和感兴趣部分的蛋白质的方法。

    Targetting adenovirus with use of constrained peptide motifs
    69.
    发明授权
    Targetting adenovirus with use of constrained peptide motifs 失效
    用限制肽基序靶向腺病毒

    公开(公告)号：US06329190B1

    公开(公告)日：2001-12-11

    申请号：US09455061

    申请日：1999-12-06

    申请人： Thomas J. Wickham ,  Petrus W. Roelvink ,  Imre Kovesdi

    发明人： Thomas J. Wickham ,  Petrus W. Roelvink ,  Imre Kovesdi

    IPC分类号： C07K14075

    CPC分类号： C12N7/00 ,  A61K38/00 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/40 ,  C12N2810/405 ,  C12N2810/60

    摘要： The present invention provides a chimeric adenovirus fiber protein, which differs from the wild-type coat protein by the introduction of a nonnative amino acid sequence in a conformationally-restrained manner. Such a chimeric adenovirus fiber protein according to the invention is able to direct entry into cells of a vector comprising the chimeric fiber protein that is more efficient than entry into cells of a vector that is identical except for comprising a wild-type adenovirus fiber protein rather than the chimeric adenovirus fiber protein. The nonnative amino acid sequences encodes a peptide motif that comprises an epitope for an antibody, or a ligand for a cell surface receptor, that can be employed in cell targeting. The present invention also pertains to vectors comprising such a chimeric adenovirus fiber protein, and to methods of using such vectors.

    摘要翻译： 本发明提供了一种嵌合腺病毒纤维蛋白，其通过以构象约束的方式引入非氨基酸序列而与野生型外壳蛋白不同。 根据本发明的这种嵌合腺病毒纤维蛋白能够直接进入包含嵌合纤维蛋白质的载体的细胞，该载体比除了包含野生型腺病毒纤维蛋白的载体的细胞进入更有效，而不是包含野生型腺病毒纤维蛋白 比嵌合腺病毒纤维蛋白。 非氨基酸序列编码包含可用于细胞靶向的抗体表位或细胞表面受体配体的肽基序。 本发明还涉及包含这种嵌合腺病毒纤维蛋白的载体，以及使用这些载体的方法。