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公开(公告)号：US06756044B1
公开(公告)日：2004-06-29
申请号：US09617569
申请日：2000-07-17
IPC分类号： A61K4500
CPC分类号： C40B40/02 , A61K47/6901 , A61K2039/5256 , A61K2039/5258 , C12N15/1037 , C12N2799/022
摘要： The present invention provides a complex that includes a virion having a ligand that recognizes an epitope present on an immune effector cell surface and at least a first nucleic acid encoding a first non-native antigen. The invention also provides a library including a plurality of such complexes, in which antigens of at least two of the plurality are different. Using such reagents, the invention provides a method of precipitating an immune response within an immune effector cell, wherein such a complex is delivered to the cell under conditions sufficient for the cell to mount an immune response to the antigen. When applied in vivo, the method can serve to immunize an animal from the pathogen. Moreover, using a library including a plurality of complexes, which contains at least one test antigen, the invention provides a method of assessing the antigenicity of the test antigen.
摘要翻译： 本发明提供了一种复合物，其包括具有识别存在于免疫效应细胞表面上的表位的配体和至少编码第一非天然抗原的第一核酸的病毒粒子。 本发明还提供了包含多个这样的复合物的文库，其中至少两个的抗原是不同的。 使用这样的试剂，本发明提供了在免疫效应细胞内沉淀免疫应答的方法，其中这种复合物在足以使细胞对抗原产生免疫应答的条件下递送至细胞。 当应用于体内时，该方法可用于从病原体免疫动物。 此外，使用包含至少一种测试抗原的多个复合物的文库，本发明提供了评估测试抗原的抗原性的方法。
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公开(公告)号：US20140314809A1
公开(公告)日：2014-10-23
申请号：US13866116
申请日：2013-04-19
申请人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
发明人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
IPC分类号： G01N33/50
CPC分类号： G01N33/505
摘要： The invention provides a method of identifying an antigen from a pathogen or a disease antigen comprising the use of an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different antigen of a pathogen. The adenoviral vectors are administered to antigen presenting cells (APCs) in vitro or to an animal in vivo. The immunogenicity of the antigen is measured by screening for an immune response from effector T lymphocytes in vitro and by screening for the absence of pathogen-induced disease onset in vivo.
摘要翻译： 本发明提供了鉴定来自病原体或疾病抗原的抗原的方法，其包括使用包含两种或多种不同腺病毒载体的腺病毒载体阵列，其中每个腺病毒载体包含编码病原体的不同抗原的核酸序列。 腺病毒载体在体外或动物体内施用于抗原呈递细胞（APC）。 通过体外筛选来自效应T淋巴细胞的免疫应答并通过筛选在体内不存在病原体诱发的疾病来测量抗原的免疫原性。
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公开(公告)号：US06455314B1
公开(公告)日：2002-09-24
申请号：US09393627
申请日：1999-09-10
IPC分类号： C12N1586
CPC分类号： C07K14/005 , A61K48/00 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2810/40 , C12N2810/405 , C12N2810/6018 , C12N2830/008
摘要： The present invention provides a recombinant protein having an amino terminus of an adenoviral fiber protein and having a trimerization domain. A fiber incorporating such a protein exhibits reduced affinity for a native substrate than does a wild-type adenoviral fiber trimer. The present invention further provides an adenovirus incorporating the recombinant protein of the present invention.
摘要翻译： 本发明提供具有腺病毒纤维蛋白的氨基末端并具有三聚结构域的重组蛋白质。 与野生型腺病毒纤维三聚体相比，掺入这种蛋白质的纤维对天然底物的亲和性降低。 本发明还提供了并入本发明的重组蛋白的腺病毒。
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4.发明授权Stocks of recombinant, replication-deficient adenovirus free of replication-competent adenovirus 失效具有无复制能力的腺病毒的重组，复制缺陷型腺病毒的库存
公开(公告)号：US5994106A
公开(公告)日：1999-11-30
申请号：US757023
申请日：1996-11-26
IPC分类号： C12N15/09 , A61K35/76 , A61K38/00 , A61K48/00 , A61P43/00 , C07K14/075 , C07K14/47 , C12N5/10 , C12N15/00 , C12N15/12 , C12N15/34 , C12N15/86 , C12N15/861 , C12Q1/68 , C12R1/92 , C12N15/64
CPC分类号： C12N7/00 , C07K14/005 , C07K14/4712 , C12N15/86 , A61K2039/5256 , A61K38/00 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352 , C12N2810/60 , C12N2810/6081 , C12N2830/002 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12N2840/44 , Y10S977/799
摘要： The present invention provides multiply deficient adenoviral vectors and complementing cell lines. Also provided are recombinants of the multiply deficient adenoviral vectors and a therapeutic method, particularly relating to gene therapy, vaccination, and the like, involving the use of such recombinants.
摘要翻译： 本发明提供多重缺陷型腺病毒载体和补体细胞系。 还提供了多重缺陷型腺病毒载体的重组体和涉及使用这种重组体的基因治疗，疫苗接种等的治疗方法。
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公开(公告)号：US06596270B2
公开(公告)日：2003-07-22
申请号：US09835683
申请日：2001-04-16
申请人： Joseph T. Bruder , Imre Kovesdi
发明人： Joseph T. Bruder , Imre Kovesdi
IPC分类号： A61K3500
CPC分类号： A61K48/00 , A61K2039/505 , C07K16/081
摘要： The present invention provides methods for administering an adenoviral gene transfer vector comprising an exogenous gene to an animal. One method involves utilizing systemic neutralizing antibodies to neutralize the adenoviral gene transfer vector outside a targeted muscle. Another method involves the repeat administration of an adenoviral gene transfer vector to a skeletal muscle.
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公开(公告)号：US06576456B2
公开(公告)日：2003-06-10
申请号：US09326447
申请日：1999-06-04
申请人： Thomas J. Wickham , Erik Falck-Pedersen , Petrus W. Roelvink , Joseph T. Bruder , Jason Gall , Imre Kovesdi
发明人： Thomas J. Wickham , Erik Falck-Pedersen , Petrus W. Roelvink , Joseph T. Bruder , Jason Gall , Imre Kovesdi
IPC分类号： C12N701
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C07K2319/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2810/405 , C12N2810/6018 , C12N2830/00 , C12N2830/60
摘要： The invention provides a chimeric adenovirus fiber protein including a nonnative amino acid sequence, and a chimeric adenovirus fiber protein lacking a native amino acid receptor-binding sequence. The chimeric protein trimerizes when produced in a mammalian cell.
摘要翻译： 本发明提供了包含非氨基酸序列的嵌合腺病毒纤维蛋白和缺乏天然氨基酸受体结合序列的嵌合腺病毒纤维蛋白。 当在哺乳动物细胞中产生时，嵌合蛋白质三聚体。
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公开(公告)号：US06482616B1
公开(公告)日：2002-11-19
申请号：US09321797
申请日：1999-05-27
IPC分类号： C12N510
CPC分类号： C12N7/00 , A61K38/00 , A61K48/00 , A61K2039/5256 , C07K14/005 , C07K14/4712 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352 , C12N2810/60 , C12N2810/6081 , C12N2830/002 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12N2840/44 , Y10S977/799
摘要： The present invention provides multiply deficient adenoviral vectors and complementing cell lines. Also provided are recombinants of the multiply deficient adenoviral vectors and a therapeutic method, particularly relating to gene therapy, vaccination, and the like, involving the use of such recombinants.
摘要翻译： 本发明提供多重缺陷型腺病毒载体和补体细胞系。 还提供了多重缺陷型腺病毒载体的重组体和涉及使用这种重组体的基因治疗，疫苗接种等的治疗方法。
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公开(公告)号：US5731190A
公开(公告)日：1998-03-24
申请号：US709515
申请日：1996-09-06
IPC分类号： C12N15/09 , A61K35/76 , A61K38/00 , A61K48/00 , C07K14/075 , C07K14/705 , C12N7/00 , C12N7/01 , C12N15/861 , C12N15/866 , C12N15/87 , C12P21/04 , C12N15/86 , C12N15/34 , C12N15/62
CPC分类号： C12N15/87 , C07K14/005 , C07K14/70546 , C12N15/86 , C12N7/00 , A61K48/00 , C07K2319/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2710/14043 , C12N2710/14143 , C12N2810/40 , C12N2810/405 , C12N2810/60 , C12N2810/6018 , C12N2810/80 , C12N2810/855 , C12N2810/856 , C12N2810/859
摘要： A recombinant adenovirus comprising a chimeric penton base protein, which includes a nonpenton base sequence, and a therapeutic gene, a method of gene therapy involving the use of such adenovirus, and adenoviral transfer vectors for the generation of such recombinant adenovirus are provided.
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公开(公告)号：US09651543B2
公开(公告)日：2017-05-16
申请号：US13866116
申请日：2013-04-19
申请人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
发明人： Joseph T. Bruder , Imre Kovesdi , Duncan L. McVey , Douglas E. Brough , C. Richter King , Denise Louise Doolan , Joao Carlos Aguair , Daniel John Carucci , Martha Sedegah , Walter R. Weiss , Keith Limbach
IPC分类号： G01N33/50
CPC分类号： G01N33/505
摘要： The invention provides a method of identifying an antigen from a pathogen or a disease antigen comprising the use of an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different antigen of a pathogen. The adenoviral vectors are administered to antigen presenting cells (APCs) in vitro or to an animal in vivo. The immunogenicity of the antigen is measured by screening for an immune response from effector T lymphocytes in vitro and by screening for the absence of pathogen-induced disease onset in vivo.
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公开(公告)号：US08765146B2
公开(公告)日：2014-07-01
申请号：US12064554
申请日：2006-08-31
申请人： Joseph T. Bruder , Imre Kovesdi , C. Richter King , Duncan L. McVey , Damodar R. Ettyreddy , Denise Louise Doolan , Daniel John Carucci
发明人： Joseph T. Bruder , Imre Kovesdi , C. Richter King , Duncan L. McVey , Damodar R. Ettyreddy , Denise Louise Doolan , Daniel John Carucci
IPC分类号： A61K39/00 , A61K39/002 , A61K39/015 , A61K39/38 , A61K45/00
CPC分类号： C07K14/445 , A61K39/015 , A61K2039/5256 , A61K2039/53 , A61K2039/545 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2830/00 , C12N2830/002 , C12N2830/008 , Y02A50/412
摘要： The invention provides adenoviral vectors comprising an adenoviral genome comprising heterologous antigen-encoding nucleic acid sequences, such as Plasmodium nucleic acid sequences, operably linked to promoters. The invention further provides a method of inducing an immune response against malaria in a mammal comprising administering the adenoviral vectors to the mammal.
摘要翻译： 本发明提供了包含腺病毒基因组的腺病毒载体，其包含可操作地连接于启动子的异源抗原编码核酸序列，例如疟原虫核酸序列。 本发明进一步提供了一种在哺乳动物中诱导针对疟疾的免疫应答的方法，包括向哺乳动物施用腺病毒载体。
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