公开(公告)号：US20230212649A1

公开(公告)日：2023-07-06

申请号：US18064956

申请日：2022-12-13

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N2458/10

Abstract: Methods of analyzing nucleic acids of a cell are provided.

公开(公告)号：US11566276B2

公开(公告)日：2023-01-31

申请号：US17366151

申请日：2021-07-02

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837 ,  G01N33/53

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US20220403313A1

公开(公告)日：2022-12-22

申请号：US17820174

申请日：2022-08-16

Applicant: President and Fellows of Harvard College

Inventor： Antonio Varone ,  Norman Wen ,  Daniel Levner ,  Richard Novak ,  Lori McPartlin ,  Donald E. Ingber ,  Youngjae Choe ,  Lian Leng ,  Justin K. Nguyen

IPC: C12M3/06 ,  C12M3/00 ,  C12M1/12 ,  C12N5/071 ,  C12N5/077

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

公开(公告)号：US20220145365A1

公开(公告)日：2022-05-12

申请号：US17584959

申请日：2022-01-26

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12P19/34

Abstract: Methods of analyzing nucleic acids of a cell are provided.

公开(公告)号：US11293054B2

公开(公告)日：2022-04-05

申请号：US16393215

申请日：2019-04-24

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Je-hyuk Lee ,  George M. Church ,  Michael Super

IPC: C12Q1/6804 ,  C12Q1/6816 ,  G01N33/53 ,  C12Q1/6837

Abstract: The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.

公开(公告)号：US20210332415A1

公开(公告)日：2021-10-28

申请号：US17366151

申请日：2021-07-02

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12P19/34 ,  C12Q1/6874

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US20210332414A1

公开(公告)日：2021-10-28

申请号：US17366127

申请日：2021-07-02

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12P19/34 ,  C12Q1/6874

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US11085911B2

公开(公告)日：2021-08-10

申请号：US16534255

申请日：2019-08-07

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Christopher David Hinojosa ,  Andries D. van der Meer ,  Marinke van der Helm ,  Abhishek Jain ,  Donald Elliot Ingber ,  Marjon Zamani

IPC: G01N33/483 ,  G01N33/50 ,  B01L3/00 ,  C12M1/00 ,  G01N15/08 ,  G01N15/00

Abstract: Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

公开(公告)号：US10814323B2

公开(公告)日：2020-10-27

申请号：US16379443

申请日：2019-04-09

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Daniel Levner ,  Guy Thompson, II ,  Christopher David Hinojosa

IPC: A61J1/06 ,  B01L3/00 ,  G01N21/00 ,  G01N27/00

Abstract: According to aspects of the present invention, a cartridge assembly for transporting fluid into or out of one or more fluidic devices includes a first layer and a second layer. The first layer includes a first surface. The first surface includes at least one partial channel disposed thereon. The second layer abuts the first surface, thereby forming a channel from the at least one partial channel. At least one of the first layer and the second layer is a resilient layer formed from a pliable material. At least one of the first layer and the second layer includes a via hole. The via hole is aligned with the channel to pass fluid thereto. The via hole is configured to pass fluid through the first layer or the second layer substantially perpendicularly to the channel. Embossments are also used to define aspects of a fluidic channel.

公开(公告)号：US10465158B2

公开(公告)日：2019-11-05

申请号：US14904640

申请日：2014-07-11

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Daniel Levner ,  Guy Thompson, II ,  Jose Fernandez-Alcon ,  Christopher David Hinojosa

IPC: G01N1/00 ,  G01N1/10 ,  B01L3/00 ,  C12M1/00 ,  C12M3/06 ,  C12M3/00 ,  C12M1/12 ,  C12M1/36 ,  B01L3/02

Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.