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112 条记录 (耗时 0.036 秒)

    Method for the enzyme-catalysed hydrolysis of polyacrylic acid esters, and esterases used therefor
    64.
    发明授权
    Method for the enzyme-catalysed hydrolysis of polyacrylic acid esters, and esterases used therefor 失效
    聚丙烯酸酯的酶催化水解方法和用于其的酯酶的方法

    公开(公告)号：US08617858B2

    公开(公告)日：2013-12-31

    申请号：US13003597

    申请日：2009-07-17

    申请人： Bernhard Hauer ,  Hans Wolfgang Höffken ,  Cecilia Kvarnström Branneby ,  Helmut Schwab ,  Sabine Feichtenhofer ,  Marlene Buchebner ,  Brigitte Pohn

    发明人： Bernhard Hauer ,  Hans Wolfgang Höffken ,  Cecilia Kvarnström Branneby ,  Helmut Schwab ,  Sabine Feichtenhofer ,  Marlene Buchebner ,  Brigitte Pohn

    IPC分类号： C12P7/52

    CPC分类号： C12P7/62 ,  C08F8/12 ,  C12N9/16 ,  C12N9/18 ,  C12Y301/01001 ,  C08F120/18

    摘要： The invention relates to a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. According to the method, at least one polyacrylic acid ester is provided and incubated with at least one enzyme selected from enzymes (EC 3.1) acting on ester bindings, until the ester groups contained in the polyacrylic acid ester are partially or fully hydrolytically split, and optionally the modified polymer obtained thereby is isolated. The invention also relates to the enzymes used and mutants thereof, nucleic acids coding for the enzymes, vectors comprising the nucleic acids, micro-organisms comprising the vectors, and the use of the enzymes, the vectors or the micro-organisms for carrying out a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. The present application also relates to polymer reaction products that can be obtained by the method, and methods for producing esterases.

    摘要翻译： 本发明涉及聚丙烯酸酯的酶催化水解方法。 根据该方法，提供至少一种聚丙烯酸酯，并与至少一种选自作用于酯结合的酶（EC 3.1）的酶进行温育，直到聚丙烯酸酯中所含的酯基部分或完全水解分裂为止，以及 由此分离获得的改性聚合物。 本发明还涉及使用的酶及其突变体，编码酶的核酸，包含核酸的载体，包含载体的微生物，以及使用酶，载体或微生物进行酶 聚丙烯酸酯酶催化水解的方法。 本申请还涉及可以通过该方法获得的聚合物反应产物和生产酯酶的方法。

    METHOD FOR ISOLATING AN ALKANOL FROM AN AQUEOUS BIOTRANSFORMATION MIXTURE
    65.
    发明申请
    METHOD FOR ISOLATING AN ALKANOL FROM AN AQUEOUS BIOTRANSFORMATION MIXTURE 审中-公开
    从水溶性生物混合物中分离出烷基醇的方法

    公开(公告)号：US20120285816A1

    公开(公告)日：2012-11-15

    申请号：US13511594

    申请日：2010-11-24

    申请人： Jürgen Däuwel ,  Michael Breuer ,  Bernhard Hauer

    发明人： Jürgen Däuwel ,  Michael Breuer ,  Bernhard Hauer

    IPC分类号： B01D3/36

    CPC分类号： C07C29/82 ,  C07B2200/07 ,  C07C29/80 ,  C07C29/86 ,  C07C31/12 ,  C07C31/125

    摘要： The invention relates to the isolation of an alkanol from an aqueous biotransformation mixture, in that a) a first alkanol phase is obtained by means of distilling out an alkanol-water azeotrope from the aqueous biotransformation mixture and, if the azeotrope is a heteroazeotrope, phase separating the azeotrope and separating out an aqueous phase, b) a second alkanol phase is obtained by (i) liquid/liquid extracting the first alkanol phase using a solvent as an extracting agent, or (ii) azeotropic drying the first alkanol phase in the presence of the solvent as a carrier agent, and c) the second alkanol phase is fractionally distilled, producing a pure alkanol fraction. The biotransformation mixture is obtained, for example, by means of reducing an alkanol in the presence of an alcohol dehydrogenase. The method is adapted to the severe dilution of the products of value in the biotransformation mixture and works without long phase separation times when extracting by means of organic solvents.

    摘要翻译： 本发明涉及从水生物转化混合物中分离链烷醇，其中a）通过从水生物转化混合物中蒸馏出链烷醇 - 水共沸物获得第一链烷醇相，如果共沸物是杂合物，则相 分离共沸物并分离水相，b）通过以下步骤获得第二链烷醇相：（i）使用溶剂作为萃取剂液/液萃取第一链烷醇相，或（ii）共沸干燥第一链烷醇相 作为载体剂的溶剂的存在，和c）分馏第二链烷醇相，产生纯的链烷醇级分。 生物转化混合物例如通过在醇脱氢酶的存在下还原链烷醇获得。 该方法适用于在生物转化混合物中严重稀释有价值的产物，并在通过有机溶剂萃取时无长时间分离时间。

    Process for esterifying or transesterifying side chains in polymers
    67.
    发明授权
    Process for esterifying or transesterifying side chains in polymers 失效
    在聚合物中酯化或酯交换侧链的方法

    公开(公告)号：US08273555B2

    公开(公告)日：2012-09-25

    申请号：US12095068

    申请日：2006-11-24

    申请人： Dietmar Haering ,  Lidcay Herrera Taboada ,  Bernhard Hauer ,  Thomas Friedrich ,  Alexander Goethlich

    发明人： Dietmar Haering ,  Lidcay Herrera Taboada ,  Bernhard Hauer ,  Thomas Friedrich ,  Alexander Goethlich

    IPC分类号： C12P7/64 ,  C12N9/00 ,  C12N9/16 ,  C12N9/20 ,  C07H21/04

    CPC分类号： C08F220/18 ,  C08F8/14 ,  C08F220/62 ,  C08F220/00

    摘要： Provided is a process for esterifying or transesterifying side chains in polymers, which involves esterifying or transesterifying the carboxyl group having the R4 radical of a copolymer obtained by polymerizing a (meth)acrylate of formula (I), and a monomer of formula (II): wherein R1 and R3 are independently H or CH3, R2 and R4 are independently H or an alkyl radical which is optionally substituted by functional groups such as acryloyl, ether, amino, epoxy, halogen or sulfonic acid groups, and n is from 1 to 40, with an alcohol in the presence of an enzyme which catalyzes the esterifying or transesterifying. Further provided is the esterified or transesterified polymer product and its use.

    摘要翻译： 本发明提供聚合物中侧链的酯化或酯交换的方法，该方法包括使通过聚合式（I）的（甲基）丙烯酸酯得到的共聚物的R4基团的羧基和式（II）的单体酯化或酯交换， ：其中R 1和R 3独立地是H或CH 3，R 2和R 4独立地是H或任选被官能团如丙烯酰基，醚，氨基，环氧基，卤素或磺酸基取代的烷基，n是1〜 40，在催化酯化或酯交换的酶存在下用醇。 进一步提供酯化或酯交换聚合物产品及其用途。