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    Preparation of beta-amino acids
    4.
    发明授权
    Preparation of beta-amino acids 有权
    β-氨基酸的制备

    公开(公告)号：US09096841B2

    公开(公告)日：2015-08-04

    申请号：US13496086

    申请日：2010-09-15

    申请人： Nina Schneider ,  Bernhard Hauer ,  Klaus Ditrich ,  Maeve O'Neil ,  Nick Turner

    发明人： Nina Schneider ,  Bernhard Hauer ,  Klaus Ditrich ,  Maeve O'Neil ,  Nick Turner

    IPC分类号： C12P13/04 ,  C12N9/86

    CPC分类号： C12N9/86 ,  C12P13/04

    摘要： The present invention relates to a process for the biocatalytic, enantioselective production of a β-amino acid precursor from an optionally substituted dihydrouracil using a hydantoinase and/or a dihydropyrimidinase, a process for producing a β-amino acid from said precursor, a hydantoinase and its use in said process for the biocatalytic production of a β-amino acid precursor or a β-amino acid, and a method for obtaining said hydantoinase.

    摘要翻译： 本发明涉及使用乙内酰脲酶和/或二氢嘧啶苷从任选取代的二氢尿嘧啶生物催化，对映选择性地生产“氨基酸”前体的方法，一种从所述前体制备一种氨基酸的方法， 乙内酰脲及其用于生物催化生产“氨基酸”前体或“氨基酸”的所述方法中的应用，以及用于获得所述乙内酰脲酶的方法。

    Process for the enzymatic reduction of enoates
    5.
    发明授权
    Process for the enzymatic reduction of enoates 有权
    烯酸酶的还原方法

    公开(公告)号：US08709767B2

    公开(公告)日：2014-04-29

    申请号：US13658328

    申请日：2012-10-23

    申请人： Stephan Maurer ,  Bernhard Hauer ,  Melanie Bonnekessel ,  Kurt Faber ,  Clemens Stückler

    发明人： Stephan Maurer ,  Bernhard Hauer ,  Melanie Bonnekessel ,  Kurt Faber ,  Clemens Stückler

    IPC分类号： C12P7/24 ,  C12P7/40 ,  C12P7/26

    CPC分类号： C12P7/24 ,  C12N9/001 ,  C12P7/22 ,  C12P7/26 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/62 ,  C12P17/10 ,  C12Y103/01031

    摘要： A process for the enzymatic reduction of an enoate (1) wherein the C═C bond of the enoate (1) is stereoselectively hydrogenated in the presence of an enoate-reductase and an oxidizable co-substrate (2) in a system which is free of NAD(P)H, in which A is a ketone radical (—CRO), an aldehyde radical (—CHO), a carboxyl radical (—COOR), with R═H or optionally substituted C1-C6-alkyl radical, R1, R2 and R3 are independently of one another H, —O—C1-C6-alkyl, —O—W with W=a hydroxyl protecting group, C1-C6-alkyl, which can be substituted, C2-C6-alkenyl, carboxyl, or an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or one of R1, R2 and R3 is a —OH radical, or R1 is linked to R3 so as to become part of a 4-8-membered cycle, or R1 is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R1, R2 and R3 may not be identical.

    摘要翻译： 在酸酐（1）的酶促还原方法中，其中将enoate（1）的C = C键在烯醇还原酶和可氧化共底物（2）存在下立体选择性氢化， 的NAD（P）H，其中A是酮基（-CRO），醛基（-CHO），羧基（-COOR），R = H或任选取代的C 1 -C 6 - 烷基，R 1 ，R2和R3彼此独立地为H，-O-C1-C6-烷基，-O-W，其中W为羟基保护基，可被取代的C1-C6-烷基，C2-C6-烯基，羧基 或任选取代的碳 - 或杂环芳族或非芳族基团，或者R 1，R 2和R 3中的一个是-OH基团，或R 1与R 3连接，以便成为4-8元环的一部分，或 R1与R连接成为4-8元环的一部分，条件是R1，R2和R3可能不相同。

    Modified nitrilases and their use in methods for the production of carboxylic acids
    9.
    发明授权
    Modified nitrilases and their use in methods for the production of carboxylic acids 有权
    改性腈水解酶及其在羧酸生产方法中的应用

    公开(公告)号：US07985572B2

    公开(公告)日：2011-07-26

    申请号：US10546611

    申请日：2004-02-24

    申请人： Thomas Zelinski ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich

    发明人： Thomas Zelinski ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich

    IPC分类号： C12N9/00 ,  C12N9/78 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/80 ,  C12N9/78 ,  C12N15/8274 ,  C12P7/40 ,  C12P7/42

    摘要： The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

    摘要翻译： 本发明涉及具有改进的基板接受性的新腈水解酶，获得它们的方法和使用上述腈水解酶。 所述腈水解酶由多肽序列编码，其在与野生型产碱杆菌粪便中的位置296相对应的位置包含不是酪氨酸的氨基酸。 本发明还涉及编码所述腈水解酶的核酸序列和氨基酸序列，包含所述核酸序列，包含核酸序列或exPression构建体的载体的表达构建体，包含核酸序列，表达构建体或生物体 向量 本发明还涉及从外消旋腈制备羧酸，优选取代的手性羧酸的方法。

    Processes for enzymatic synthesis of poly(oxyalkylene)acrylamides
    10.
    发明授权
    Processes for enzymatic synthesis of poly(oxyalkylene)acrylamides 有权
    用于酶合成聚（氧化烯）丙烯酰胺的方法

    公开(公告)号：US07906596B2

    公开(公告)日：2011-03-15

    申请号：US11912203

    申请日：2006-04-20

    申请人： Dietmar Häring ,  Bernhard Hauer ,  Stefan Becker

    发明人： Dietmar Häring ,  Bernhard Hauer ,  Stefan Becker

    IPC分类号： C12P13/02

    CPC分类号： C12P13/02 ,  C08F220/58 ,  C08F283/06 ,  C08G65/332 ,  C08G65/33324

    摘要： Processes for enzymatic synthesis comprising: (a) providing an aliphatic poly(oxyalkylene)amine; and (b) reacting the aliphatic poly(oxyalkylene)amine with a reactant selected from the group consisting of acrylic acid compounds and alkyl esters thereof, in the presence of a hydrolase in bulk or in a liquid reaction medium comprising an organic solvent to for a poly(oxyalkylene)acrylamide.

    摘要翻译： 用于酶促合成的方法包括：（a）提供脂族聚（氧亚烷基）胺; 和（b）使脂族聚（氧化烯）胺与选自丙烯酸化合物及其烷基酯的反应物在本体水解酶存在下或在包含有机溶剂的液体反应介质中反应，以使 聚（氧化烯）丙烯酰胺。