公开(公告)号：US20230233655A1

公开(公告)日：2023-07-27

申请号：US18194954

申请日：2023-04-03

申请人： Arthrex, Inc.

发明人： Christopher Bare ,  Abigail Nabors ,  Melissa Tucker ,  Robert Harrison

IPC分类号： A61K38/48 ,  B01L3/00 ,  C12N9/74

CPC分类号： A61K38/4833 ,  B01L3/5021 ,  B01L3/50825 ,  C12N9/6429 ,  C12Y304/21005 ,  B01L2300/0609 ,  B01L2300/041 ,  B01L2300/047 ,  B01L2300/026 ,  B01L2200/026 ,  B01L2300/042 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2300/16

摘要： A system for preparing a thrombin serum that can include a containment device, a cage received within the containment device, a cap attachable to the containment device, an inlet port configured to introduce a non-anti-coagulated autologous blood fluid into the containment device, and an outlet port. An activator, such as glass beads, can be present within the containment device.

公开(公告)号：US11519905B2

公开(公告)日：2022-12-06

申请号：US15947644

申请日：2018-04-06

申请人： Massachusetts Institute of Technology

发明人： Sangeeta N. Bhatia ,  Jaideep S. Dudani ,  Ester J. Kwon ,  Andrew David Warren

IPC分类号： C12Q1/68 ,  C12Q1/00 ,  G01N33/574 ,  G01N33/53 ,  G01N33/543 ,  A61K49/00 ,  G01N33/542 ,  C07K19/00 ,  C12Q1/37 ,  G01N33/58 ,  A61K47/68 ,  A61K49/18 ,  C07K14/705 ,  C12N9/74 ,  C12N9/64 ,  G01N33/553 ,  C07K14/47

摘要： Aspects of the disclosure relate to methods and compositions useful for in vivo and/or in vitro enzyme profiling. In some embodiments, the disclosure provides methods of in vivo enzymatic processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. In some embodiments, the disclosure provides compositions and in vitro methods for localization of enzymatic activity in a tissue sample.

公开(公告)号：US20220034853A1

公开(公告)日：2022-02-03

申请号：US17389382

申请日：2021-07-30

申请人： Shandong Institute for Food and Drug Control ,  Shandong University ,  Avanc Pharmaceutical Co.,Ltd

发明人： Ruiqing Xian ,  Feng Shi ,  Liping Gong ,  Lianli Chi ,  Qunye Zhang ,  Weijian Wang ,  Hongming Du ,  Congcong Wang ,  Baojian Hang ,  Fengshan Wang

IPC分类号： G01N30/06 ,  G01N30/72 ,  C12N9/74

摘要： The present invention provides a snake venom thrombin marker peptide of Agkistrodon Halys Pallas and an application of the snake venom thrombin-like enzyme in identifying species of Hemocoagulase for Injection. The application includes the following steps of: dissolving a to-be-detected sample and a reference substance of the marker peptide respectively to prepare a test solution and a reference solution, and conducting alkylation reduction on the test solution and the reference solution with dithiothreitol and iodoacetamide; after diluting products with an ammonium bicarbonate solution, adding enzyme for hydrolysis; and after enzymolysis is finished, conducting centrifugation at a high speed, and injecting a supernatan into a liquid chromatography-mass spectrometer for analysis. This method is simple, convenient and rapid, is strong in specificity, fills the gap in identifying the source of species of the snake venom thrombin-like enzyme of Agkistrodon Halys Pallas and improves the quality control level.

公开(公告)号：US20210268077A1

公开(公告)日：2021-09-02

申请号：US17325358

申请日：2021-05-20

申请人： ARTHREX, INC.

发明人： Christopher BARE ,  Abigail Nabors ,  Melissa Tucker ,  Robert Harrison

IPC分类号： A61K38/48 ,  B01L3/00 ,  C12N9/74

摘要： A system for preparing a thrombin serum that can include a containment device, a cage received within the containment device, a cap attachable to the containment device, an inlet port configured to introduce a non-anti-coagulated autologous blood fluid into the containment device, and an outlet port. An activator, such as glass beads, can be present within the containment device.

公开(公告)号：US10760068B2

公开(公告)日：2020-09-01

申请号：US16320164

申请日：2017-07-20

申请人： QGEL SA

发明人： Simone Rizzi ,  Jeremy Touati

IPC分类号： C12N9/74 ,  C12N5/00

摘要： A hydrogel precursor formulation which is in the form of an unreacted powder. The formulation comprises an activating enzyme, preferably thrombin, a cross-linking enzyme, preferably a transglutaminase, and more preferably factor XIII transglutaminase. The cross-linking enzyme is activatable by the activating enzyme in water with or without a buffer, and at least one structural compound A. The structural compound is crosslinkable by a selective reaction mediated by the crosslinking enzyme to form a hydrogel, wherein the cross-linking enzyme is activated.

公开(公告)号：US20200172915A1

公开(公告)日：2020-06-04

申请号：US16790430

申请日：2020-02-13

申请人： AbSci LLC

发明人： Sean McClain ,  Mark Valasek

IPC分类号： C12N15/70 ,  C12P21/02 ,  C07K16/24 ,  C07K16/28 ,  C12N9/74

摘要： The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.

公开(公告)号：US20200157153A1

公开(公告)日：2020-05-21

申请号：US16632941

申请日：2018-07-24

申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA

发明人： Claudia Schmidt-Dannert ,  Maureen B. Quin ,  Guoqiang Zhang ,  Kelly Kristen Wallin

IPC分类号： C07K14/255 ,  C12N15/62 ,  C12N9/74 ,  C12N9/96 ,  C12N9/06 ,  C07K14/32

摘要： A protein scaffold includes a plurality of EutM subunits and a multi-enzyme cascade. The multi-enzyme cascade includes a first enzyme attached to the first EutM subunit and a second enzyme attached to the second EutM subunit. The scaffold may be formed by a method that generally includes incubating a plurality of EutM subunits under conditions allowing the EutM subunits to self-assemble into a protein scaffold, attaching a first enzyme of a multi-enzyme cascade to a first EutM subunit, and attaching a second enzyme of the multi-enzyme cascade to a second EutM subunit. The scaffold may be self-assembled in vivo or in vitro. Each enzyme may be, independently of any other enzyme, attached to its EutM subunit in vivo or in vitro. Each enzyme may be, independently of any other enzyme, attached to its EutM subunit before or after the scaffold is assembled.

公开(公告)号：US20190153419A1

公开(公告)日：2019-05-23

申请号：US16097485

申请日：2017-04-28

申请人： Saint Louis University

发明人： Enrico Di Cera ,  Sergio Barranco-Medina ,  Nicola Pozzi

IPC分类号： C12N9/74 ,  C07K14/745 ,  A61K45/06 ,  A61K38/48 ,  A61K38/36 ,  A61K9/00 ,  A61P7/02

摘要： Compositions and methods for regulating the blood coagulation pathway are disclosed. More particularly, the present disclosure relates to thrombin-thrombomodulin fusion proteins and their use as blood anticoagulants and for treating sepsis.

公开(公告)号：US20190105370A1

公开(公告)日：2019-04-11

申请号：US16170131

申请日：2018-10-25

申请人： GLADIATOR BIOSCIENCES, INC.

发明人： Maxine BAUZON ,  Terry HERMISTON

IPC分类号： A61K38/17 ,  C12N9/64 ,  A61N5/10 ,  C07K14/745 ,  C07K14/485 ,  A61K38/16 ,  A61K38/18 ,  A61K45/06 ,  A61K47/64 ,  C12N9/74

摘要： The disclosure relates to the recombinant Gla domain proteins and their use targeting phosphatidylserine (PtdS) moieties on the surface of cells, particularly those expressing elevated levels of PtdS, such as cells undergoing apoptosis.

公开(公告)号：US10131932B2

公开(公告)日：2018-11-20

申请号：US15066509

申请日：2016-03-10

申请人： Shengjun Qiao ,  Peter Gross

发明人： Shengjun Qiao ,  Peter Gross

IPC分类号： C12N9/00 ,  C12Q1/56 ,  C12Q1/37 ,  G01N33/542 ,  C12N9/12 ,  C12N9/64 ,  C12N9/74 ,  G01N21/64 ,  G01N33/58

摘要： A method of determining generation of an activated protease in a biological sample is provided. The method comprises the steps of exposing a biological sample to a substrate for the activated protease, wherein the substrate comprises a detectable label linked to a cleavage sequence for the activated protease by C-terminal and N-terminal spacers that form a beta-sheet, and wherein the detectable label emits a first signal associated with the substrate and second signal associated with a cleaved product; and determining the generation of the activated protease by measuring the change in the first or second signal over time.