公开(公告)号：US20200232032A1

公开(公告)日：2020-07-23

申请号：US16747737

申请日：2020-01-21

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Katherine Lazaruk ,  Dennis E. Prosen ,  Devon Jensen ,  David Rosenfeld ,  Janice H. Lai ,  James Ghadiali ,  Christina Chang

IPC: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6853

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

公开(公告)号：US20200071691A1

公开(公告)日：2020-03-05

申请号：US16551620

申请日：2019-08-26

Applicant: Cellular Research, Inc.

Inventor： Margaret Nakamoto ,  Eleen Shum

IPC: C12N15/10 ,  C12Q1/6881

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a carbohydrate binding reagent or a cell membrane permeable reagent associated with an oligonucleotide, such as a sample indexing oligonucleotide. Different oligonucleotides can have different sequences. Sample origin of cells can be determined based on the sequences of the oligonucleotides by, for example, barcoding the oligonucleotides.

公开(公告)号：US20190292592A1

公开(公告)日：2019-09-26

申请号：US16374626

申请日：2019-04-03

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn Fu ,  Craig Betts

IPC: C12Q1/6874 ,  G16B25/00 ,  C12N15/10 ,  C12Q1/6806

Abstract: This disclosure provides methods and compositions for removing one or more high abundance species from a plurality of nucleic acid molecules. In some embodiments, the methods and compositions can be used for normalizing nucleic acid libraries. In some embodiments, molecular labels are used in conjunction with the methods and compositions disclosed herein to improve sequencing efficiency.

公开(公告)号：USD851275S1

公开(公告)日：2019-06-11

申请号：US29614032

申请日：2017-08-15

Applicant: Cellular Research, Inc.

Designer： Philipp S. Spuhler ,  Sixing Li ,  Christopher G. Cesar ,  Geoffrey R. Facer ,  Janice H. Lai

公开(公告)号：USD851150S1

公开(公告)日：2019-06-11

申请号：US29614039

申请日：2017-08-15

Applicant: Cellular Research, Inc.

Designer： Philipp S. Spuhler ,  Christopher G. Cesar ,  Janice H. Lai ,  Geoffrey R. Facer ,  Sixing Li

公开(公告)号：US20190119726A1

公开(公告)日：2019-04-25

申请号：US16232287

申请日：2018-12-26

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12Q1/686

Abstract: Disclosed herein are methods and systems for correcting errors in sample amplification, including the errors occurred in determining the number of targets in samples. In some embodiments, the method comprises: stochastically barcoding a plurality of targets in the samples using oligonucleotides comprising stochastic barcodes to generate stochastically barcoded targets; contacting one or more defined barcoded primers with each of the one or more samples; and determining an amplification noise.

公开(公告)号：US20180346969A1

公开(公告)日：2018-12-06

申请号：US16012635

申请日：2018-06-19

Applicant: CELLULAR RESEARCH, INC.

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum

IPC: C12Q1/6811 ,  C12Q1/6806 ,  C12Q1/6816 ,  G01N33/532 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6876 ,  C12Q1/6804 ,  C12Q1/6834

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

公开(公告)号：US10059991B2

公开(公告)日：2018-08-28

申请号：US15217896

申请日：2016-07-22

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Glenn K. Fu

IPC: C12N15/10 ,  G06F19/20 ,  G06F19/22 ,  C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q1/6851

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B25/00 ,  G16B30/00 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US20180200710A1

公开(公告)日：2018-07-19

申请号：US15868811

申请日：2018-01-11

Applicant: Cellular Research, Inc.

Inventor： Philipp Spuhler ,  Sixing Li

IPC: B01L3/00 ,  C12N15/10 ,  G01N15/10

Abstract: Disclosed herein are methods, devices, and systems for efficient loading and retrieval of particles. In some embodiments, a fluidic channel of a flowcell comprises a ceiling, a first sidewall, and a bottom, wherein the contact angle of the ceiling is at least 10 degrees smaller than the contact angle of the first sidewall, and wherein the bottom of the fluidic channel comprises a substrate that comprises a plurality of microwells.

公开(公告)号：US20180037942A1

公开(公告)日：2018-02-08

申请号：US15665906

申请日：2017-08-01

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6874 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2565/514

Abstract: The present disclosure relates to compositions, methods and kits for quantitative analysis of a plurality of nucleic acid target molecules in a sample. In some embodiments, the methods comprise associating molecular label sequences with target nucleic acid molecules without an enzymatic reaction.