公开(公告)号：US20050074454A1

公开(公告)日：2005-04-07

申请号：US10957549

申请日：2004-09-30

Applicant: Junming Le ,  Jan Vilcek ,  Peter Daddona ,  John Ghrayeb ,  David Knight ,  Scott Siegel

Inventor： Junming Le ,  Jan Vilcek ,  Peter Daddona ,  John Ghrayeb ,  David Knight ,  Scott Siegel

IPC: A61K38/00 ,  C07K14/525 ,  C07K16/24 ,  C07K16/30 ,  A61K39/395

CPC classification number: C07K16/241 ,  A61K31/167 ,  A61K31/192 ,  A61K31/196 ,  A61K31/405 ,  A61K31/485 ,  A61K31/573 ,  A61K38/00 ,  A61K39/3955 ,  A61K2039/505 ,  C07K14/525 ,  C07K16/30 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/76 ,  C07K2319/00 ,  A61K2300/00

Abstract: Anti-TNF antibodies, fragments and regions thereof which are specific for human tumor necrosis factor-α (TNFα) and are useful in vivo diagnosis and therapy of a number of TNFα-mediated pathologies and conditions, as well as polynucleotides coding for murine and chimeric antibodies, methods of producing the antibody, methods of use of the anti-TNF antibody, or fragment, region or derivative thereof, in immunoassays and immunotherapeutic approaches are provided.

Abstract translation: 抗TNF抗体，其对人肿瘤坏死因子-α（TNFalpha）特异性的片段和区域，并且可用于许多TNFα介导的病理学和病症的体内诊断和治疗，以及编码鼠和嵌合体的多核苷酸 提供了免疫测定和免疫治疗方法中的抗体，抗体的制备方法，抗TNF抗体的使用方法或其片段，区域或衍生物。

公开(公告)号：US06835823B2

公开(公告)日：2004-12-28

申请号：US09756398

申请日：2001-01-08

Applicant: Jumming Le ,  Jan Vilcek ,  Peter Daddona ,  John Ghrayeb ,  David Knight ,  Scott Siegel

Inventor： Jumming Le ,  Jan Vilcek ,  Peter Daddona ,  John Ghrayeb ,  David Knight ,  Scott Siegel

IPC: C07H2104

CPC classification number: A61K39/3955 ,  A61K31/167 ,  A61K31/192 ,  A61K31/196 ,  A61K31/405 ,  A61K31/485 ,  A61K31/519 ,  A61K31/573 ,  A61K38/00 ,  A61K45/00 ,  A61K45/06 ,  A61K2039/505 ,  C07K14/525 ,  C07K16/241 ,  C07K16/30 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/00 ,  A61K2300/00

Abstract: Anti-TNF antibodies, fragments and regions thereof which are specific for human tumor necrosis factor-&agr; (TNF&agr;) and are useful in vivo diagnosis and therapy of a number of TNF&agr;-mediated pathologies and conditions, as well as polynucleotides coding for murine and chimeric antibodies, methods of producing the antibody, methods of use of the anti-TNF antibody, or fragment, region or derivative thereof, in immunoassays and immunotherapeutic approaches are provided.

Abstract translation: 抗TNF抗体，其对人肿瘤坏死因子-α（TNFalpha）特异性的片段和区域，并且可用于许多TNFα介导的病理学和病症的体内诊断和治疗，以及编码鼠和嵌合体的多核苷酸 提供了免疫测定和免疫治疗方法中的抗体，抗体的制备方法，抗TNF抗体的使用方法或其片段，区域或衍生物。

公开(公告)号：US06284471B1

公开(公告)日：2001-09-04

申请号：US08192093

申请日：1994-02-04

Applicant: Junming Le ,  Jan Vilcek ,  Peter Dadonna ,  John Ghrayeb ,  David Knight ,  Scott A. Siegel

Inventor： Junming Le ,  Jan Vilcek ,  Peter Dadonna ,  John Ghrayeb ,  David Knight ,  Scott A. Siegel

IPC: C07K1624

CPC classification number: C07K14/525 ,  A61K38/00 ,  A61K2039/505 ,  C07K16/241 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/00

Abstract: Anti-TNF antibodies and anti-TNF peptides, specific for tumor necrosis factor (TNF) are useful for in vivo diagnosis and therapy of a number of TNF-mediated pathologies and conditions, as well as polynucleotides coding for anti-TNF murine and chimeric antibodies, peptides, methods of making and using the antibody or peptides in immunoassays and immuno-therapeutic approaches are provided, where the anti-TNF peptide is selected from a soluble portion of TNF receptor, an anti-TNF antibody or structural analog thereof.

Abstract translation: 特异于肿瘤坏死因子（TNF）的抗TNF抗体和抗TNF肽可用于许多TNF介导的病理学和病症的体内诊断和治疗，以及编码抗TNF鼠和嵌合抗体的多核苷酸 提供肽，免疫测定法和免疫治疗方法中制备和使用抗体或肽的方法，其中抗TNF肽选自TNF受体的可溶性部分，抗TNF抗体或其结构类似物。

公开(公告)号：US5063053A

公开(公告)日：1991-11-05

申请号：US556999

申请日：1990-07-25

Applicant: Flossie Wong-Stal ,  Pranab K. Chanda ,  John Ghrayeb

Inventor： Flossie Wong-Stal ,  Pranab K. Chanda ,  John Ghrayeb

IPC: A61K38/00 ,  C07K14/16 ,  C07K16/10

CPC classification number: C07K14/005 ,  C07K16/10 ,  A61K38/00 ,  C12N2740/16322

Abstract: The isolation and purification of a newly discovered gene of the AIDS virus, HTLV-III, which encodes a portein which is immunogenic and recognized by sera of some HTLV-III seropositive people. Furthermore, the gene is highly conserved among all known HTLV-III isolates and exhibits a polymorphism at the 3' end which distinguishes molecular clones of the HTLV-IIIB cell line from viral genomes of related viruses.

Abstract translation: 分离和纯化新发现的艾滋病病毒基因HTLV-III，其编码一种免疫原性并被一些HTLV-III血清阳性人的血清识别的portein。 此外，该基因在所有已知的HTLV-III分离物中是高度保守的，并且在3'末端显示出多态性，其区分HTLV-IIIB细胞系的分子克隆与相关病毒的病毒基因组。

公开(公告)号：US4910132A

公开(公告)日：1990-03-20

申请号：US49051

申请日：1987-05-12

Applicant: David M. Knight ,  John Ghrayeb

Inventor： David M. Knight ,  John Ghrayeb

IPC: G01N33/569

CPC classification number: G01N33/56988

Abstract: A virus-free assay to identify agents that interfere with the life cycle of HIV which comprises: (1) expressing in mammalian cells in the presence or absence of one or more of said agents, HIV gp120, the gene product of HIV tat, the gene product of HIV art/trs, all under the control of an HIV LTR promoter, and a control protein, not under the control of the HIV LTR promoter, such that infectious HIV particles are not produced; (2) quantitating the amount of the gp120 produced relative to the amount of control protein produced; and (3) comparing the relative amount of the gp120 produced in the presence of said one or more agents to the relative amount of the gp120 produced in the absence of said one or more agents. A plasmid capable of expressing the gene product of HIV art/trs in mammalian cells such that infectious HIV particles are not produced, which includes: (1) a DNA fragment comprising an HIV LTR promoter, and (2) and HIV cDNA restriction fragment carrying the art/trs gene but not the ATG start codon of the first coding exon for tat.

Abstract translation: 用于鉴定干扰HIV生命周期的试剂的无病毒测定法，其包括：（1）在存在或不存在一种或多种所述药物HIV gp120，HIV tat的基因产物， 全部由HIV LTR启动子控制的HIV技术/ trs的基因产物和不在HIV LTR启动子控制下的对照蛋白，使得不产生感染性HIV颗粒; （2）定量相对于产生的对照蛋白的量产生的gp120的量; 和（3）将所述一种或多种试剂存在下产生的gp120的相对量与在所述一种或多种试剂不存在下产生的gp120的相对量进行比较。 能够在哺乳动物细胞中表达HIV art / trs的基因产物的质粒，使得不产生感染性HIV颗粒，其包括：（1）包含HIV LTR启动子的DNA片段，和（2）携带 艺术/ trs基因，但不是第一个编码外显子的ATG起始密码子。

公开(公告)号：US4808536A

公开(公告)日：1989-02-28

申请号：US834212

申请日：1986-02-27

Applicant: Nancy T. Chang ,  John Ghrayeb

Inventor： Nancy T. Chang ,  John Ghrayeb

IPC: A61K38/00 ,  A61K39/00 ,  A61K39/21 ,  C07K14/00 ,  C07K14/005 ,  C07K14/16 ,  C07K14/195 ,  C07K16/00 ,  C07K19/00 ,  C12N15/09 ,  C12P21/00 ,  C12R1/19 ,  G01N33/569 ,  G01N33/545 ,  B65D69/00 ,  C12Q1/70

CPC classification number: C07K14/005 ,  G01N33/56988 ,  A61K38/00 ,  C12N2740/16222 ,  Y10S435/81 ,  Y10S435/974 ,  Y10S436/808 ,  Y10S436/811 ,  Y10S530/826 ,  Y10S930/221

Abstract: Immunochemical assays for detection of antibodies against HTLV-III core proteins are described. The assays are based upon recombinant HTLV-III core proteins expressed by cloned DNA segments of the gag region of the HTLV-III genome. Immunoreactive, chimeric HTLV-III core proteins and methods of producing these proteins are also described.

Abstract translation: 描述了用于检测针对HTLV-III核心蛋白的抗体的免疫化学测定。 该测定基于由HTLV-III基因组的gag区域的克隆DNA片段表达的重组HTLV-III核心蛋白。 还描述了免疫反应性，嵌合HTLV-III核心蛋白质和产生这些蛋白质的方法。