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    Amylase variants
    71.
    发明申请
    Amylase variants 审中-公开

    公开(公告)号：US20090280527A1

    公开(公告)日：2009-11-12

    申请号：US11235738

    申请日：2005-09-21

    申请人： Henrik Bisgard-Frantzen ,  Allan Svendsen ,  Torben Borchert

    发明人： Henrik Bisgard-Frantzen ,  Allan Svendsen ,  Torben Borchert

    IPC分类号： C12P21/06 ,  C13K1/06 ,  C12P7/08 ,  C12N9/26 ,  C12N1/21 ,  C12N1/19 ,  C12N15/63 ,  C07H21/04

    CPC分类号： C11D3/38618 ,  C11D3/38609 ,  C11D3/38681 ,  C12N9/2417 ,  C12Y302/01001 ,  D06L1/14

    摘要： The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant:(a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.

    Amylases for Pharmaceutical Use
    73.
    发明申请
    Amylases for Pharmaceutical Use 失效
    药用淀粉酶

    公开(公告)号：US20090035293A1

    公开(公告)日：2009-02-05

    申请号：US11917551

    申请日：2006-06-16

    申请人： Allan Svendsen ,  Peter Collin Gregory

    发明人： Allan Svendsen ,  Peter Collin Gregory

    IPC分类号： A61K38/43 ,  C12N9/26 ,  A61P1/00

    CPC分类号： C12N9/2417

    摘要： The pharmaceutical use of amylases related to Bacillus alpha-amylases of SEQ ID NOs: 1-3, optionally in combination with a lipase and/or a protease. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The amylases of SEQ ID NOs: 1-3 are variants of amylases from Bacillus stearothermophilus, Bacillus licheniformis and Bacillus sp. The amylases of the invention have an improved efficacy in vivo, an improved pH-profile, a high specific activity, and/or an improved starch degradation profile.

    摘要翻译： 与SEQ ID NO：1-3的芽孢杆菌α-淀粉酶相关的淀粉酶的药物用途，任选地与脂肪酶和/或蛋白酶组合。 医学适应症的例子有：消化系统疾病，胰腺外分泌功能不全（PEI），胰腺炎，囊性纤维化，I型糖尿病和/或II型糖尿病的治疗。 SEQ ID NO：1-3的淀粉酶是来自嗜热脂肪芽孢杆菌，地衣芽孢杆菌和芽孢杆菌属的淀粉酶的变体。 本发明的淀粉酶在体内具有改善的功效，改善的pH分布，高比活性和/或改进的淀粉降解特征。

    PHYTASE VARIANTS
    74.
    发明申请
    PHYTASE VARIANTS 审中-公开

    公开(公告)号：US20090029004A1

    公开(公告)日：2009-01-29

    申请号：US11753660

    申请日：2007-05-25

    申请人： Tomoko Matsui ,  Claus Crone Fuglsang ,  Allan Svendsen ,  Shiro Fukuyama

    发明人： Tomoko Matsui ,  Claus Crone Fuglsang ,  Allan Svendsen ,  Shiro Fukuyama

    IPC分类号： A23K1/00 ,  C12N9/14 ,  A01H5/00 ,  C12N9/50 ,  C12P21/04 ,  C12N15/11 ,  C12N15/00 ,  C12N5/04

    CPC分类号： C12N9/16 ,  A23K10/14 ,  A23K20/189

    摘要： The present invention relates to phytase variants derived from a parent phytase which is homologous to a Peniophora lycii phytase and comprises at least one substitution in at least one of a number of positions corresponding to a position in the Peniophora lycii phytase. The variants are of improved properties, in particular of a higher specific activity and/or more thermostable than the parent phytase, e.g., of a Tm of up to 82° C. at pH 5.5, as determined by DSC, and/or of a doubted specific activity. The invention also relates to DNA encoding the phytase variants, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及衍生自母本植酸酶的植酸酶变体，所述植酸酶与青i i植酸酶同源，并且包括至少一个位于对应于Peniophora lycii植酸酶中的位置的位置中的至少一个取代。 这些变体具有改进的性质，特别是比母体植酸酶更高的比活性和/或更高的热稳定性，例如，通过DSC测定的在pH5.5达到82℃的Tm，和/或 怀疑具体活动。 本发明还涉及编码植酸酶变体的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

    Proteases for Pharmaceutical Use
    75.
    发明申请
    Proteases for Pharmaceutical Use 审中-公开
    药用蛋白酶

    公开(公告)号：US20080317726A1

    公开(公告)日：2008-12-25

    申请号：US11917595

    申请日：2006-06-16

    申请人： Allan Svendsen ,  Claus Crone Fuglsang ,  Peter Colin Gregory

    发明人： Allan Svendsen ,  Claus Crone Fuglsang ,  Peter Colin Gregory

    IPC分类号： C12N9/50 ,  A61K38/48 ,  A61P3/10 ,  A61P1/18 ,  A61P11/00 ,  A61K38/54

    CPC分类号： C12N9/54

    摘要： The pharmaceutical use of proteases related to amino acids 1-274 of SEQ ID NO: 2, the serine protease derived from Bacillus licheniformis, which is also designated subtilisin Carlsberg, optionally in combination with a lipase and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II.

    摘要翻译： 与SEQ ID NO：2的氨基酸1-274相关的蛋白酶的药物用途，来自地衣芽孢杆菌的丝氨酸蛋白酶，其也被指定为枯草杆菌蛋白酶卡尔斯伯格，任选与脂肪酶和/或淀粉酶组合。 医学适应症的例子有：消化系统疾病，胰腺外分泌功能不全（PEI），胰腺炎，囊性纤维化，I型糖尿病和/或II型糖尿病的治疗。

    Enzymatic Enantioselective Ester or Amide Hydrolysis or Synthesis
    77.
    发明申请
    Enzymatic Enantioselective Ester or Amide Hydrolysis or Synthesis 审中-公开
    酶对映选择性酯或酰胺水解或合成

    公开(公告)号：US20080138856A1

    公开(公告)日：2008-06-12

    申请号：US11813614

    申请日：2006-02-10

    申请人： Allan Svendsen ,  Jesper Vind ,  Jesper Brask ,  Leonardo De Maria

    发明人： Allan Svendsen ,  Jesper Vind ,  Jesper Brask ,  Leonardo De Maria

    IPC分类号： C12P21/06 ,  C12P7/62

    CPC分类号： C12N9/20 ,  C07K2299/00 ,  C12P7/02 ,  C12P7/40 ,  C12P7/62 ,  C12P13/02 ,  C12P41/004 ,  C12P41/007

    摘要： The enantioselectivity of fungal lipolytic enzymes can be altered by substituting a suitably selected amino acid residue. The residue to be substituted is selected from its location in the 3D structure of the enzyme and an ester substrate (or a substrate analogue). A residue in the lid may be selected if it is located close to the acid part or close to the alcohol part of an ester substrate. A residue outside the lid region may be selected if it is located close to the active site or close to the substrate.

    摘要翻译： 真菌脂肪分解酶的对映体选择性可以通过替代适当选择的氨基酸残基来改变。 待取代的残基选自其酶的位置和酯底物（或底物类似物）的3D结构。 如果位于靠近酸性部分或靠近酯基材的醇部分的位置，则可以选择盖子中的残余物。 如果位于靠近活性部位或靠近基底的位置，则可以选择盖子外部的残余物。

    Starch debranching enzymes
    78.
    发明授权
    Starch debranching enzymes 失效
    淀粉脱支酶

    公开(公告)号：US07374922B2

    公开(公告)日：2008-05-20

    申请号：US10375720

    申请日：2003-02-26

    申请人： Henrik Bisgard-Frantzen ,  Allan Svendsen

    发明人： Henrik Bisgard-Frantzen ,  Allan Svendsen

    IPC分类号： C12N9/44 ,  C12N15/00 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C12N9/2457 ,  C12N9/2451 ,  C12N9/246 ,  C12P19/16 ,  C12Y302/01041 ,  C12Y302/01068

    摘要： The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.

    摘要翻译： 本发明涉及母体淀粉脱支酶的遗传工程改造，即支链淀粉酶或淀粉酶，所述酶变体在与亲本酶相比在4-6的范围内具有改善的热稳定性和/或增加的活性 对于支链淀粉和/或糖原，与制备具有改进的热稳定性和/或改变的底物特异性的淀粉脱支酶变体的方法以及使用至少一种这样的酶变体将淀粉转化为一种或多种糖的方法 。