POLYMERASE STABILIZATION
    79.
    发明申请
    POLYMERASE STABILIZATION 审中-公开
    聚合物稳定

    公开(公告)号:US20100159528A1

    公开(公告)日:2010-06-24

    申请号:US12601102

    申请日:2008-06-12

    IPC分类号: C12P19/34 C12N9/96

    CPC分类号: C12N9/96 C12N9/1252

    摘要: The present invention relates to methods and compositions for providing purified thermostable enzymes, particularly thermostable DNA polymerases, that are free of exogenous detergents. The present invention also provides methods for providing such purified thermostable DNA polymerases to assays in an active form by adding one or more detergents. The present invention further provides compositions and kits comprising purified thermostable DNA polymerases for use in a variety of applications, including amplification and sequencing of nucleic acids.

    摘要翻译: 本发明涉及用于提供不含外源洗涤剂的纯化的热稳定酶,特别是耐热性DNA聚合酶的方法和组合物。 本发明还提供了通过添加一种或多种洗涤剂来提供这种纯化的热稳定性DNA聚合酶以活性形式的测定法的方法。 本发明进一步提供了包含用于各种应用的纯化的热稳定性DNA聚合酶的组合物和试剂盒,包括核酸的扩增和测序。